A simple quantitative method for the determination of small amounts of amino-acids

The Analyst ◽  
1967 ◽  
Vol 92 (1099) ◽  
pp. 627 ◽  
Author(s):  
J. G. Heathcote ◽  
R. J. Washington
Keyword(s):  
Amino Acids ◽  
Quantitative Method

scholarly journals A colorimetric micro method for the determination of formyl groups

1969 ◽  
Vol 111 (4) ◽  
pp. 401-406 ◽  
Author(s):  
S. Usha Lakshmi ◽  
L. K. Ramachandran
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Chromatographic Separation ◽  
Quantitative Method ◽  
Standard Procedure ◽  
Formyl Group ◽  
Alumina Column ◽  
Purple Colour ◽  
Paper Chromatographic Separation

The characteristic purple colour formed by N-formyl-N′-2,4-dinitrophenyl-hydrazine in the presence of piperidine and acetone was made the basis of a new quantitative method for the determination of formyl groups. Samples containing N-formyl groups (up to 0·4μmole) are hydrazinolysed at 97–98° for 1hr. and are dinitrophenylated after the removal of excess of hydrazine. Interference from 2,4-dinitrophenylhydrazine is eliminated by subjecting the dinitrophenylated samples to chromatography on an alumina column. Interference arising from the formation of N-acetyl-N′-2,4-dinitrophenylhydrazine, when determining formyl groups in samples containing acetyl, can be avoided by a paper-chromatographic separation before analysis. A standard procedure is described. The method gives satisfactory results when applied to N-formyl-amino acids. Gramicidin, when analysed by this method, was found to contain 0·89 mole of formyl group/mole for a molecular weight of 1880. The method indicated the absence of formyl groups from lysozyme, a protein known not to contain such groups. Generally, the analytical values obtained by the method are within 100±4% of theory.

AN ULTRAMICRO METHOD FOR QUANTITATIVE DETERMINATION OF AMINO ACIDS

1952 ◽  
Vol 30 (8) ◽  
pp. 581-591 ◽  
Author(s):  
Eunice F. Wellington
Keyword(s):  
Amino Acids ◽  
Quantitative Method ◽  
Environmental Temperature ◽  
Complete Oxidation ◽  
Two Dimensional ◽  
Color Intensity ◽  
Color Development ◽  
Concentration Time

A simple, quantitative method for determination of amino acids in about 300 µgm. protein is described. The amino acids are separated by two-dimensional paper chromatography. The chromatograms are sprayed with 2% ninhydrin and the color is developed under controlled and standardized conditions. The colored areas are cut out, eluted, and the color intensity is read in a photometer. The following factors have been shown to affect the color reaction of ninhydrin with amino acids on paper chromatograms: the quality of the ninhydrin; its concentration; time of color development; and the environmental temperature and humidity. Complete oxidation of the hydrolyzate is necessary for accurate quantitative determinations of cysteine, cystine, glutamic acid, aspartic acid, methionine, and valine. Strict standardization of the length of the solvent run is also important for reproducibility in the analyses of some amino acids.Bovine serum albumin has been analyzed by this method and the results found to be in excellent agreement with previously published values.

PROFITABILITY ANALYSIS OF TERUBUK FARMING (SACCHARUM EDULE HASSKARL) IN SUBDISTRICT TOSA DISTRICT OF EAST TIDORE OF TIDORE ISLAND

2018 ◽  
Author(s):  
Suwandi S. Sangadji
Keyword(s):  
Data Collection ◽  
Research Method ◽  
Quantitative Method ◽  
Secondary Data ◽  
Primary Data ◽  
Profitability Analysis ◽  
Sample Method ◽  
Document Review ◽  
Revenue Production

The purpose of this researchment is to ascertain how wide the farming of species Saccharun Edule Hasskarl (terubuk) in sub district Tosa, district of East Tidore of Tidore Island through the indicator of the value revenue, production and selling prices so that the farmers will achieve The Break Event Point (BEP). The research method was used a quantitative method with the number of samples of 30 people. The determination of the sample method is using the census method or involving all members of the population into a sample of researchment. The secondary data collection was done by using library literature in the form of document review and relevant references to research object while primary data collection was done by using questionnaire. The data is using equation R /C Ratio, BEP Revenue, BEP Price, and BEP Production. Therefore from the results of the researchment it can be explained that the two of the thirty farmers come through the break event point, while the other twenty-eight farmers declared having a business that worth to be develop or experiencing profit, because the R/C ratio is above 1.0 with average profit reach Rp. 989.000, - per production / farmer.

scholarly journals Rapid Organocatalytic Chirality Analysis of Amines, Amino Acids, Alcohols, Amino Alcohols and Diols with Achiral Iso(thio)cyanate Probes

2021 ◽  
Author(s):  
Eryn Nelson ◽  
Jeffrey S. S. K. Formen ◽  
Christian Wolf
Keyword(s):  
Amino Acids ◽  
Absolute Configuration ◽  
Rapid Determination ◽  
Amino Alcohols ◽  
Enantioselective Synthesis ◽  
Widespread Occurrence ◽  
Chiral Compounds ◽  
New Methods ◽  
The Absolute

The widespread occurrence and significance of chiral compounds does not only require new methods for their enantioselective synthesis but also efficient tools that allow rapid determination of the absolute configuration,...

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