A proposed nucleotide sequence for the 5S ribosomal ribonucleic acid of rainbow trout (Salmo gairdneri)

1978 ◽  
Vol 56 (1) ◽  
pp. 60-65 ◽  
Author(s):  
Kenneth L. Roy
Keyword(s):  
Rainbow Trout ◽  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Gel Electrophoresis ◽  
Inorganic Phosphate ◽  
Polyacrylamide Gel Electrophoresis ◽  
Salmo Gairdneri ◽  
Ribosomal Ribonucleic Acid ◽  
Complete Digestion ◽  
Pancreatic Rnase

Rainbow trout cell cultures have been exposed to 32P-labelled inorganic phosphate and the labelled RNA has been isolated. The 5S ribosomal ribonucleic acid (5S rRNA) was purified by polyacrylamide gel electrophoresis, then digested with RNase T1 or pancreatic RNase. The products of complete digestion were separated and their sequences determined. These analyses have allowed a sequence to be proposed which differs in eight positions from that of mammalian 5S rRNAs.

scholarly journals The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Products of complete digestion with pancreatic ribonuclease and ribonuclease T1

1976 ◽  
Vol 153 (2) ◽  
pp. 437-446 ◽  
Author(s):  
N J Holness ◽  
G Atfield
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Brief Treatment ◽  
Ribonuclease T1 ◽  
Pancreatic Ribonuclease ◽  
Adenylic Acid ◽  
Complete Digestion ◽  
Conventional Methods

1. The nucleotide chain of tRNA Cys from baker's yeast was readily split at the anticolon into two large fragments by brief treatment with ribonuclease T1.2. The whole molecule and the two derived large fragments were completely digested with (a) pancreatic ribonuclease and (b) ribonuclease T1. The fragments present in each of the digests were separated and sequenced by conventional methods. 3. The groups of fragments derived from the two methods of digestion were entirely compatible with each other. 4. The molecule is 75 nucleotides long, but, as isolated, lacks the terminal adenosine and the neighboring cytidylic acid residue. The minor nucleotides 1-methyladenylic acid, 7-methylguanylic acid, 5-methylcytidylic acid and N6 (γγ-dimethylallyl)adenylic acid (isopentenyladenylic acid) were identified.

The soluble adenosine triphosphatase of Thiobacillus ferrooxidans

1975 ◽  
Vol 21 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Catherine Adapoe ◽  
Marvin Silver
Keyword(s):  
Enzyme Activity ◽  
Atpase Activity ◽  
Gel Electrophoresis ◽  
Inorganic Phosphate ◽  
Adenosine Triphosphatase ◽  
Thiobacillus Ferrooxidans ◽  
Polyacrylamide Gel Electrophoresis ◽  
Histochemical Analysis ◽  
Ph Optimum ◽  
Major Band

Adenosine triphosphatase (ATPase) from Thiobacilhis ferrooxidans was purified 55-fold. Polyacrylamide gel electrophoresis of the most purified fraction showed only one major band; histochemical analysis showed that the ATPase activity was associated with this band. The pH optimum is 9–10. The enzyme hydrolyzed ATP stoichiometrically to ADP and inorganic phosphate, the Km for this substrate being 7.75 × 10−3 M. GTP and ITP are alternate substrates, the Km values for these being 6.71 × 10−3 M and 3.12 × 10−3 M, respectively. ADP is slightly hydrolyzed. Magnesium, manganese, and calcium can serve as cofactors; Km values for these are 2.0 × 10−3 M, 9.4 × 10−4 M, and 8.0 × 10−4 M, respectively. The enzyme activity was not activated by either sodium or potassium, but a combination of the two ions were inhibitory. Azide and p-hydroxymercuribenzoate strongly inhibited the enzyme activity, whereas cyanide, dinitrophenol, and N, N′-dicyclohexylcarbodiimide (DCCD) were without effect. The enzyme was cold labile at 0 °C, but was more stable at 18–24 °C.

The nucleotide sequence of dolphin and bovine 5S ribosomal ribonucleic acid

FEBS Letters ◽  
1975 ◽  
Vol 54 (2) ◽  
pp. 241-244 ◽  
Author(s):  
T.A. Walker ◽  
J.L. Betz ◽  
J. Olah ◽  
N.R. Pace
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Ribosomal Ribonucleic Acid

scholarly journals Nucleotide sequence of 5S ribosomal ribonucleic acid from a sulfate-reducing bacterium, Desulfovibrio vulgaris.

1986 ◽  
Vol 34 (10) ◽  
pp. 4190-4194 ◽  
Author(s):  
KAZUNOBU MIURA ◽  
JUNJI KAKUCHI ◽  
ERIKO ENDO ◽  
TOHRU UEDA ◽  
KUNIHIKO KOBAYASHI ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Desulfovibrio Vulgaris ◽  
Sulfate Reducing ◽  
Ribosomal Ribonucleic Acid

Nucleotide sequence of the Dictyostelium discoideum small-subunit ribosomal ribonucleic acid inferred from the gene sequence: evolutionary implications

Biochemistry ◽  
1983 ◽  
Vol 22 (25) ◽  
pp. 5858-5868 ◽  
Author(s):  
Robert McCarroll ◽  
Gary J. Olsen ◽  
Yvonne D. Stahl ◽  
Carl R. Woese ◽  
Mitchell L. Sogin
Keyword(s):  
Nucleotide Sequence ◽  
Dictyostelium Discoideum ◽  
Ribonucleic Acid ◽  
Gene Sequence ◽  
Small Subunit ◽  
Ribosomal Ribonucleic Acid
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