scholarly journals The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Products of complete digestion with pancreatic ribonuclease and ribonuclease T1

1976 ◽  
Vol 153 (2) ◽  
pp. 437-446 ◽  
Author(s):  
N J Holness ◽  
G Atfield
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Brief Treatment ◽  
Ribonuclease T1 ◽  
Pancreatic Ribonuclease ◽  
Adenylic Acid ◽  
Complete Digestion ◽  
Conventional Methods

1. The nucleotide chain of tRNA Cys from baker's yeast was readily split at the anticolon into two large fragments by brief treatment with ribonuclease T1.2. The whole molecule and the two derived large fragments were completely digested with (a) pancreatic ribonuclease and (b) ribonuclease T1. The fragments present in each of the digests were separated and sequenced by conventional methods. 3. The groups of fragments derived from the two methods of digestion were entirely compatible with each other. 4. The molecule is 75 nucleotides long, but, as isolated, lacks the terminal adenosine and the neighboring cytidylic acid residue. The minor nucleotides 1-methyladenylic acid, 7-methylguanylic acid, 5-methylcytidylic acid and N6 (γγ-dimethylallyl)adenylic acid (isopentenyladenylic acid) were identified.

scholarly journals The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Identification of the products from partial degradation of the molecule and derivation of the complete sequence

1976 ◽  
Vol 153 (2) ◽  
pp. 447-454 ◽  
Author(s):  
N J Holness ◽  
G Atfield
Keyword(s):  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Complete Sequence ◽  
Baker’S Yeast ◽  
Baker's Yeast ◽  
Brief Treatment ◽  
Yeast Identification ◽  
Pancreatic Ribonuclease ◽  
Correct Sequence ◽  
Partial Degradation

1. A series of large oligonucleotide fragments derived from tRNA Cys, were separated chromatographically and the sequence of each was deduced by examination of the products of digestion with pancreatic and T1 ribonucleases. 2. The location of the specific cleavage points in the nucleotide chain was similar to that produced by brief treatment with pancreatic ribonuclease. 3. The fragments could be arranged into two alternative sequences. The correct sequence was deduced by the sequential removal and identification of the first nine nucleotides from the 3′-end of the terminal half of the molecules.

The corrected nucleotide séquence of valine tRNA from baker's yeast

Biochimie ◽  
1974 ◽  
Vol 56 (9) ◽  
pp. 1211-1213 ◽  
Author(s):  
J. Bonnet ◽  
J.P. Ebel ◽  
G. Dirheimer ◽  
L.P. Shershneva ◽  
A.I. Krutlina ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Baker’S Yeast ◽  
Baker's Yeast

Isolation of Seryl and Phenylalanyl Ribonucleic Acid Synthetases from Baker's Yeast*

Biochemistry ◽  
1965 ◽  
Vol 4 (7) ◽  
pp. 1434-1442 ◽  
Author(s):  
Maynard H. Makman ◽  
Giulio L. Cantoni
Keyword(s):  
Ribonucleic Acid ◽  
Baker’S Yeast ◽  
Baker's Yeast

A proposed nucleotide sequence for the 5S ribosomal ribonucleic acid of rainbow trout (Salmo gairdneri)

1978 ◽  
Vol 56 (1) ◽  
pp. 60-65 ◽  
Author(s):  
Kenneth L. Roy
Keyword(s):  
Rainbow Trout ◽  
Nucleotide Sequence ◽  
Ribonucleic Acid ◽  
Gel Electrophoresis ◽  
Inorganic Phosphate ◽  
Polyacrylamide Gel Electrophoresis ◽  
Salmo Gairdneri ◽  
Ribosomal Ribonucleic Acid ◽  
Complete Digestion ◽  
Pancreatic Rnase

Rainbow trout cell cultures have been exposed to 32P-labelled inorganic phosphate and the labelled RNA has been isolated. The 5S ribosomal ribonucleic acid (5S rRNA) was purified by polyacrylamide gel electrophoresis, then digested with RNase T1 or pancreatic RNase. The products of complete digestion were separated and their sequences determined. These analyses have allowed a sequence to be proposed which differs in eight positions from that of mammalian 5S rRNAs.

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