Nucleotide sequence of the Dictyostelium discoideum small-subunit ribosomal ribonucleic acid inferred from the gene sequence: evolutionary implications

Biochemistry ◽  
1983 ◽  
Vol 22 (25) ◽  
pp. 5858-5868 ◽  
Author(s):  
Robert McCarroll ◽  
Gary J. Olsen ◽  
Yvonne D. Stahl ◽  
Carl R. Woese ◽  
Mitchell L. Sogin
FEBS Letters ◽  
1975 ◽  
Vol 54 (2) ◽  
pp. 241-244 ◽  
Author(s):  
T.A. Walker ◽  
J.L. Betz ◽  
J. Olah ◽  
N.R. Pace

1986 ◽  
Vol 34 (10) ◽  
pp. 4190-4194 ◽  
Author(s):  
KAZUNOBU MIURA ◽  
JUNJI KAKUCHI ◽  
ERIKO ENDO ◽  
TOHRU UEDA ◽  
KUNIHIKO KOBAYASHI ◽  
...  

1978 ◽  
Vol 56 (1) ◽  
pp. 60-65 ◽  
Author(s):  
Kenneth L. Roy

Rainbow trout cell cultures have been exposed to 32P-labelled inorganic phosphate and the labelled RNA has been isolated. The 5S ribosomal ribonucleic acid (5S rRNA) was purified by polyacrylamide gel electrophoresis, then digested with RNase T1 or pancreatic RNase. The products of complete digestion were separated and their sequences determined. These analyses have allowed a sequence to be proposed which differs in eight positions from that of mammalian 5S rRNAs.


1981 ◽  
Vol 1 (1) ◽  
pp. 35-42 ◽  
Author(s):  
G Mangiarotti ◽  
F Altruda ◽  
H F Lodish

Synthesis of ribosomes and ribosomal ribonucleic acid (RNA) continued during differentiation of Dictyostelium discoideum concurrently with extensive turnover of ribosomes synthesized during both growth and developmental stages. We show here that the rate of synthesis of 26S and 17S ribosomal RNA during differentiation was less than 15% of that in growing cells, and by the time of sorocarp formation only about 25% of the cellular ribosomes had been synthesized during differentiation. Ribosomes synthesized during growth and differentiation were utilized in messenger RNA translation to the same extent; about 50% of each class were on polyribosomes. Ribosome degradation is apparently an all-or-nothing process, since virtually all 80S monosomes present in developing cells could be incorporated into polysomes when growth conditions were restored. By several criteria, ribosomes synthesized during growth and differentiation were functionally indistinguishable. Our data, together with previously published information on changes in the messenger RNA population during differentiation, indicate that synthesis of new ribosomes is not necessary for translation of developmentally regulated messenger RNA. We also establish that the overall rate of messenger RNA synthesis during differentiation is less than 15% of that in growing cells.


1980 ◽  
Vol 8 (21) ◽  
pp. 4911-4917 ◽  
Author(s):  
Ron M. MacKay ◽  
Michael W. Gray ◽  
W.Ford Doolittle

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