The Hepatic Chalone. I. Assay Method for the Hormone and Purification of the Rabbit Liver Chalone

1971 ◽  
Vol 49 (12) ◽  
pp. 1376-1383 ◽  
Author(s):  
W. G. Verly ◽  
Y. Deschamps ◽  
J. Pushpathadam ◽  
M. Desrosiers
Keyword(s):  
Molecular Weight ◽  
Dna Synthesis ◽  
Rabbit Liver ◽  
Dose Effect ◽  
Assay Method ◽  
Small Molecular Weight ◽  
Tissue Specific ◽  
Effect Relationship ◽  
Receptor Sites ◽  
Species Specific

Chalone from rabbit liver has been purified 450-fold; it is a polypeptide of small molecular weight. Its action on liver DNA synthesis is tissue-specific but not species-specific. The dose–effect relationship is in agreement with the theory of receptor sites in the hepatocytes with a low affinity for this hormone.

scholarly journals Inhibition of Growth of Hair Follicles by a Lectin-like Substance from Rat Skin

1983 ◽  
Vol 36 (4) ◽  
pp. 411 ◽  
Author(s):  
R Frater
Keyword(s):  
Molecular Weight ◽  
Epithelial Cells ◽  
Dna Synthesis ◽  
Affinity Chromatography ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hair Follicles ◽  
Rat Skin ◽  
Small Molecular Weight ◽  
Inhibition Of Growth

A small molecular weight (5000--10000) substance has been isolated from rat skin by affinity chromatography on a column of acid-hydrolysed Sepharose. The substance agglutinates rabbit red blood cells, inhibits DNA synthesis in rat hair follicles, and causes the appearance of autophagic vacuoles in the epithelial cells of the lower follicle bulb.

Tissue and species-specific effects of small molecular weight nuclear RNA's on transcription in isolated mammalian nuclei

1981 ◽  
Vol 59 (5) ◽  
pp. 343-352 ◽  
Author(s):  
Wing-Cheong Liu ◽  
Roseline Godbout ◽  
Ernest Jay ◽  
Kenneth K.-Y. Yu ◽  
Margarida O. Krause
Keyword(s):  
Molecular Weight ◽  
Human Cells ◽  
Kidney Cells ◽  
Small Molecular Weight ◽  
Isolated Nuclei ◽  
Specific Effects ◽  
Specific Manner ◽  
Normal Human ◽  
Species Specific ◽  
Slab Gels

Small molecular weight nuclear RNA's (SnRNA's) purified from the 0.35 M NaCl extract of chromatin from human and monkey tissues have been found to stimulate transcription of chromatin in isolated nuclei in a tissue- and species-specific manner. While SnRNA from normal human cells (WI38 fibroblasts and placenta) stimulates homologous transcription to some extent, it has a greater activity on transcription of heterologous tissue of the same species and no activity on heterologous tissue of a different species (monkey kidney cells). Likewise SnRNA from monkey cells stimulates transcription of homologous chromatin but has no effect on human cells. Fractionation of the RNA's in polyacrylamide gradient slab gels revealed that in all cases the active RNA was 160–175 nucleotides in length. Our results are compatible with the hypothesis that the active RNA's are involved in the determination and maintenance of tissue differentiation by recognizing promoter or regulator sequences in the DNA and act at the level of the nucleosome to induce tissue-specific genes.

Hemostatically potent small molecular weight serine protease from Maclura spinosa (Roxb. ex Willd.) accelerates healing of subcutaneous dermal wounds in Swiss albino mice

Biologia ◽  
2019 ◽  
Vol 75 (1) ◽  
pp. 139-149
Author(s):  
Venkatesh Bommalapura Kulkarni ◽  
Raghu Ram Achar ◽  
Maheshwari Mahadevappa ◽  
Dinesh Sosalagere Manjegowda ◽  
Priya Babu Shubha ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Serine Protease ◽  
Small Molecular Weight ◽  
Swiss Albino Mice ◽  
Albino Mice

Pronucleus formation, DNA synthesis and metaphase entry in porcine oocytes following intracytoplasmic injection of murine spermatozoa

Zygote ◽  
2003 ◽  
Vol 11 (3) ◽  
pp. 261-270 ◽  
Author(s):  
Bong-Ki Kim ◽  
Sun Hong Cheon ◽  
Youn Jeong Lee ◽  
Sun Ho Choi ◽  
Xiang Shun Cui ◽  
...  
Keyword(s):  
Dna Synthesis ◽  
Normal Cell ◽  
S Phase ◽  
Normal Male ◽  
Ultrastructural Observation ◽  
Intracytoplasmic Injection ◽  
Paternal Factors ◽  
The Mean ◽  
Species Specific ◽  
Mean Time

The onset of pronucleus formation and DNA synthesis in porcine oocytes following the injection of porcine or murine sperm was determined in order to obtain insights into species-specific paternal factors that contribute to fertilisation. Similar frequencies of oocytes with female pronuclei were observed after injection with porcine sperm or with murine sperm. In contrast, male pronuclei formed 8-9 h following the injection of porcine sperm, and 6-8 h following the injection of murine sperm. After pronucleus formation maternally derived microtubules were assembled and appeared to move both male and female pronuclei to the oocyte centre. A few porcine oocytes entered metaphase 22 h after the injection of murine sperm, but normal cell division was not observed. The mean time of onset of S-phase in male pronuclei was 9.7 h following porcine sperm injection and 7.4 h following mouse sperm injection. Ultrastructural observation revealed that male pronuclei derived from murine sperm in porcine oocytes are morphologically similar to normal male pronuclei in porcine zygotes. These results suggest that species-specific paternal factors influence the onset of pronucleus formation and DNA synthesis. However, normal nuclear cytoplasmic interactions were observed in porcine S-phase oocytes following murine sperm injection.

Biosynthesis of the Diguanosine Nucleotides. I. Purification and Properties of an Enzyme from Yolk Platelets of Brine Shrimp Embryos

1974 ◽  
Vol 52 (3) ◽  
pp. 231-240 ◽  
Author(s):  
A. H. Warner ◽  
P. C. Beers ◽  
F. L. Huang
Keyword(s):  
Molecular Weight ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Temperature Optimum ◽  
Small Molecular Weight ◽  
Ph Optimum ◽  
Optimal Activity ◽  
Purification And Properties

An enzyme that catalyzes the synthesis of P1P4-diguanosine 5′-tetraphosphate (Gp4G) has been isolated and purified from yolk platelets of encysted embryos of the brine shrimp, Artemia salina. The enzyme GTP:GTP guanylyltransferase (Gp4G synthetase) utilizes GTP as substrate, has a pH optimum of 5.9–6.0, a temperature optimum of 40–42 °C, and requires Mg2+ and dithiothreitol for optimal activity. The synthesis of Gp4G is inhibited markedly by pyrophosphate, whereas orthophosphate has no effect on the reaction. In the presence of GDP the enzyme also catalyzes the synthesis of P1,P3-diguanosine 5′-triphosphate (Gp3G), but the rate of synthesis is low compared with Gp4G synthesis and dependent upon other small molecular weight components of yolk platelets.

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