scholarly journals Inhibition of Growth of Hair Follicles by a Lectin-like Substance from Rat Skin

1983 ◽  
Vol 36 (4) ◽  
pp. 411 ◽  
Author(s):  
R Frater
Keyword(s):  
Molecular Weight ◽  
Epithelial Cells ◽  
Dna Synthesis ◽  
Affinity Chromatography ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hair Follicles ◽  
Rat Skin ◽  
Small Molecular Weight ◽  
Inhibition Of Growth

A small molecular weight (5000--10000) substance has been isolated from rat skin by affinity chromatography on a column of acid-hydrolysed Sepharose. The substance agglutinates rabbit red blood cells, inhibits DNA synthesis in rat hair follicles, and causes the appearance of autophagic vacuoles in the epithelial cells of the lower follicle bulb.

scholarly journals Cold Steeping Infusion, a Novel Lectin Extraction Technique for the Isolation, Purification and Partial Characterization of Lectins from the Green Venezuelan Marine Alga Caulerpa serrulata

2018 ◽  
Vol 13 (12) ◽  
pp. 1934578X1801301
Author(s):  
Pablo Djabayan-Djibeyan ◽  
Brian Carpenter ◽  
Gerardo Medina-Ramírez ◽  
Felix Andueza-Leal ◽  
Andrés León-Leal ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Red Blood Cells ◽  
Ammonium Sulfate ◽  
Bioactive Compounds ◽  
Blood Cells ◽  
Marine Alga ◽  
Continuous Production ◽  
Isotonic Saline ◽  
Size Exclusion ◽  
Extraction Technique

A lectin from the green Venezuelan marine alga Caulerpa serrulata was extracted with phosphate buffered saline (PBS) using cold steeping infusion (CSI) and by grinding with liquid nitrogen (GLN). The proteins were precipitated using solid ammonium sulfate. Both the crude extracts and ammonium sulfate precipitated proteins were tested for hemagglutinins using native and papain-treated human red blood cell suspensions in isotonic saline solution. Purification of lectins was achieved using affinity chromatography-sugar-epoxy-sepharose 6B and molecular weight was assessed by size exclusion chromatography using Bio-gel® P-100 and SDS-PAGE with 2-mercaptoethanol. IEF-urea 8M was also evaluated. Using CSI it was shown that the marine alga released hemagglutinating compounds into the solutions; the same hemagglutinating compounds were also obtained by GLN. Ammonium sulfate precipitated proteins exhibited agglutinating activity against native and papain-treated human red blood cells. Temperature and EDTA were shown to affect dramatically the lectin activity towards red blood cells. A lectin was purified efficiently and the molecular weight calculated as approximately 78,000 Daltons. The CSI technique demonstrated that the alga could be returned to an active metabolic state by immersion in a simple buffer after having been kept dormant by freezing at −20°C for long periods. It was also shown that the alga was releasing bioactive compounds into the solutions and, therefore, this procedure is being suggested as a good, gentle, non-disruptive extraction technique and we postulate CSI as a possible bioreactor for the continuous production of bioactive compounds from green marine algae.

The Hepatic Chalone. I. Assay Method for the Hormone and Purification of the Rabbit Liver Chalone

1971 ◽  
Vol 49 (12) ◽  
pp. 1376-1383 ◽  
Author(s):  
W. G. Verly ◽  
Y. Deschamps ◽  
J. Pushpathadam ◽  
M. Desrosiers
Keyword(s):  
Molecular Weight ◽  
Dna Synthesis ◽  
Rabbit Liver ◽  
Dose Effect ◽  
Assay Method ◽  
Small Molecular Weight ◽  
Tissue Specific ◽  
Effect Relationship ◽  
Receptor Sites ◽  
Species Specific

Chalone from rabbit liver has been purified 450-fold; it is a polypeptide of small molecular weight. Its action on liver DNA synthesis is tissue-specific but not species-specific. The dose–effect relationship is in agreement with the theory of receptor sites in the hepatocytes with a low affinity for this hormone.

Chloride and taurine effluxes occur by different pathways in skate erythrocytes

1996 ◽  
Vol 271 (6) ◽  
pp. R1544-R1549 ◽  
Author(s):  
E. M. Davis-Amaral ◽  
M. W. Musch ◽  
L. Goldstein
Keyword(s):  
Epithelial Cells ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Disulfonic Acid ◽  
Raja Erinacea ◽  
Western Blots ◽  
Cl Channel ◽  
Cl Channels

The aim of this study was to determine whether volume-activated taurine and Cl- effluxes occur via the same system in skate (Raja erinacea) red blood cells (RBC). The effluxes were measured in isotonic and hypotonic elasmobranch Ringer solutions, in which NaCl was replaced by mannitol and the remaining exchangeable anions with gluconate. Methazolamide (0.1 mM) was added to minimize HCO3- formation. RBC Cl- content fell approximately 50%/h in both isotonic and hypotonic media, with no detectable K- loss in either medium. The observed Cl- loss was accompanied by an increase in pH. Both the Cl- loss and pH rise were inhibited by 4,4'- diisothiocyanostilbene-2,2'-disulfonic acid (0.1 mM), suggesting that Cl- efflux was due to H(+)-Cl- cotransport. 36Cl- effluxes in isotonic and hypotonic media were (means +/- SE, n = 11) 2.8 +/- 0.6 and 3.5 +/- 0.9 mumol.g dry wt RBC-1.min-1, respectively, whereas [3H]taurine effluxes in the same media were 0.045 +/- 0.02 and 2.1 +/- 0.05 mumol.g dry wt RBC-1.min-1, respectively (n = 6). These results indicate that taurine and Cl- effluxes occur via different pathways in skate RBC. In addition, the swelling-activated Cl- channel reported in epithelial cells does not appear to be present in skate RBC. This conclusion was confirmed by Western blots with an antibody to swelling-activated Cl- channels. Taurine and Cl- fluxes are apparently under different pathway influences in these RBC: taurine diffuses via a channel, whereas Cl- is transported by cotransporters.

scholarly journals Histological investigation on Ancyrocephalus paradoxus (Dactylogyridea: Ancyrocephalidae) infection causing mortalities in an intensively cultured pikeperch [Sander lucioperca (L.)] stock

2016 ◽  
Vol 64 (2) ◽  
pp. 201-212
Author(s):  
Kálmán Molnár ◽  
Gábor Szilágyi ◽  
Gábor Mosonyi ◽  
Ádám Varga ◽  
Csaba Székely
Keyword(s):  
Epithelial Cells ◽  
Connective Tissue ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Histological Investigation ◽  
Pathological Changes ◽  
Sander Lucioperca ◽  
Gill Parasite ◽  
Attachment Sites ◽  
Gill Filaments

In a cultured pikeperch (Sander lucioperca) stock the monopisthocotylean monogenean gill parasite Ancyrocephalus paradoxus caused heavy infection and mortalities. The gills of the affected fish specimens were infected by 50 to 800 monogenean parasites. Severe pathological changes were found in areas where the worms attached to the gills. At the attachment sites the haptoral discs of the worms formed a deep depression in the epithelium of the filaments, and the anchors pierced into and fixed themselves to the connective tissue of the cartilaginous gill rays. At these attachment sites red blood cells released from injured capillaries were found among the damaged epithelial cells. Around the hooks, anchors and body sections coming into contact with the gill filaments a proliferative tissue developed in which only a remnant of the damaged lamellae was found. Due to the damage caused by the worms the tips of the heavily infected gill filaments fused, formed clubs and were composed of epitheloid-type regeneration tissue lacking respiratory lamellae. In the basal parts of the filaments, where most of the worms attached to the gill, only denuded filaments deprived of lamellae were observed among the cross-sectioned worms in histological sections.

scholarly journals Pneumatic tube transportation of urine samples

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Eline Sandvig Andersen ◽  
Ivan Brandslund
Keyword(s):  
Epithelial Cells ◽  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Urine Samples ◽  
Tube System ◽  
Pneumatic Tube ◽  
Local Quality ◽  
Renal Tubular ◽  
Pneumatic Tube System

AbstractObjectivesPneumatic tube transportation of samples is an effective way of reducing turn-around-time, but evidence of the effect of pneumatic tube transportation on urine samples is lacking. We thus wished to investigate the effect of pneumatic tube transportation on various components in urine, in order to determine if pneumatic tube transportation of these samples is feasible.MethodsOne-hundred fresh urine samples were collected in outpatient clinics and partitioned with one partition being carried by courier to the laboratory, while the other was sent by pneumatic tube system (Tempus600). Both partitions were then analysed for soluble components and particles, and the resulting mean difference and limits of agreement were calculated.ResultsAlbumin, urea nitrogen, creatinine, protein and squamous epithelial cells were unaffected by transportation in the Tempus600 system, while bacteria, renal tubular epithelial cells, white blood cells and red blood cells were affected and potassium and sodium may have been affected.ConclusionsThough pneumatic tube transportation did affect some of the investigated components, in most cases the changes induced were clinically acceptable, and hence samples could be safely transported by the Tempus600 pneumatic tube system. For bacteria, white blood cells and red blood cells local quality demands will determine if pneumatic tube transportation is appropriate.

scholarly journals A simple method for the determination of bacterial sensitivity to sulphonamides by the use of blotting-paper disks

1948 ◽  
Vol 46 (4) ◽  
pp. 422-425 ◽  
Author(s):  
R. J. Evans
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Nutrient Agar ◽  
Zone Of Inhibition ◽  
Simple Method ◽  
Blotting Paper ◽  
Inhibition Of Growth ◽  
Bacterial Sensitivity ◽  
Horse Red Blood Cells

A description is given of a simple method of determining the sensitivity of bacteria to sulphonamides by means of impregnated blotting-paper disks applied to nutrient agar prepared from broth freed from sulphonamide antagonists by incubation with lysed horse red blood cells.With organisms sensitive to the selected sulphonamide, a zone of inhibition of growth will occur, the diameter of which is related to the concentration of the sulphonamide necessary to inhibit growth. Sensitivity to several sulphonamides can be determined simultaneously.The impregnated disks can be stored without loss of potency.

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