Oxidation processes in relation to the induction of enzymes in Jerusalem artichoke tuber slices

1970 ◽  
Vol 48 (3) ◽  
pp. 316-321 ◽  
Author(s):  
Marcel Bastin ◽  
Huguette Dijkmans
Keyword(s):  
Oxygen Uptake ◽  
Jerusalem Artichoke ◽  
Phenol Oxidase ◽  
Carbonyl Groups ◽  
Oxidation Processes ◽  
Jerusalem Artichoke Tuber ◽  
Induction Of Enzymes ◽  
Ethylenediamine Tetraacetate

The effect of diethyldithiocarbamate (DIECA) and ethylenediamine tetraacetate (EDTA) on the wounding-dependent induction of phenol oxidase and peroxidase in Jerusalem artichoke tuber slices has been investigated. DIECA which is a good inhibitor of oxidase reactions inhibits the induction of enzymes. EDTA which does not affect these reactions prevents the induction only at a concentration of 5 μmoles/ml. At a concentration of 1 μmole/ml, however, which stimulates the rate of oxygen uptake by the slices, EDTA also stimulates the induction of enzymes.The induction of enzymes was enhanced when slices were cultured in the presence of compounds having two neighboring carbonyl groups (dehydroascorbate, pyruvate, and oxalate).The rate of both induction and oxygen uptake increased with increasing intensity of slicing.

Study on the inactivation of enzymes in Jerusalem artichoke tuber slices

1972 ◽  
Vol 50 (4) ◽  
pp. 727-731 ◽  
Author(s):  
Marcel Bastin ◽  
Osman Ünlüer
Keyword(s):  
Half Life ◽  
Jerusalem Artichoke ◽  
Phenol Oxidase ◽  
Enzyme Synthesis ◽  
Strong Inhibitor ◽  
First Order ◽  
Jerusalem Artichoke Tuber ◽  
Enzyme Degradation ◽  
Inhibitor Of Protein Synthesis ◽  
Stimulation Of

The formation of both peroxidase and phenol oxidase was induced by culturing slices of Jerusalem artichoke tubers under aerobic conditions at 30 °C. The rate of enzyme degradation in the tuber slices was measured after applying cycloheximide, a strong inhibitor of protein synthesis, or by culturing the tissues under anaerobic conditions. Peroxidase decayed according to a first order process with a half-life of 8.3 h while phenol oxidase was found to be more stable (half-life about 70 h). The increase in the level of enzymes after the slicing treatment was achieved through a stimulation of the rate of enzyme synthesis rather than a decrease in the rate of destruction.

Action des rayons gamma du Cobalt 60 sur la teneur en acides nucléiques et l'histogenèse des tissus de tubercule de topinambour cultivés in vitro

1983 ◽  
Vol 61 (5) ◽  
pp. 1448-1455 ◽  
Author(s):  
Janine Schaeverbeke-Sacré ◽  
Béatrice Matheron
Keyword(s):  
Radiation Dose ◽  
Gamma Irradiation ◽  
Gamma Rays ◽  
Cytological Study ◽  
Jerusalem Artichoke ◽  
Dna And Rna ◽  
Jerusalem Artichoke Tuber ◽  
Outer Area

DNA and RNA contents are studied in Jerusalem artichoke tuber explants cultured in vitro after gamma irradiation (0–5 × 105 rads (1 rad = 10 mGy)). The lower part of the explants is stimulated as soon as in contact with the medium. This stimulated area is still able to synthesize DNA and RNA up to 104 rads. An histological and cytological study shows that tissue neoformations can be observed up to 6000 rads in this outer area and that gamma rays seem to keep the cells in a "premitotic" state for a longer or shorter period according to the applied radiation dose.

Effects of 2,4- and 3,5-dichlorophenoxyacetic acids on Jerusalem artichoke tuber tissue disks

1969 ◽  
Vol 8 (10) ◽  
pp. 1859-1866 ◽  
Author(s):  
P.P. Rutherford ◽  
E.W. Weston ◽  
A.E. Flood
Keyword(s):  
Jerusalem Artichoke ◽  
Tuber Tissue ◽  
Jerusalem Artichoke Tuber
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