Ribosome metabolism in hormone-treated Jerusalem artichoke tuber slices in the absence and presence of 5-fluorouracil

Planta ◽  
1977 ◽  
Vol 135 (3) ◽  
pp. 267-273 ◽  
Author(s):  
J. Sparkuhl ◽  
G. Setterfield
Keyword(s):  
Jerusalem Artichoke ◽  
Jerusalem Artichoke Tuber

Action des rayons gamma du Cobalt 60 sur la teneur en acides nucléiques et l'histogenèse des tissus de tubercule de topinambour cultivés in vitro

1983 ◽  
Vol 61 (5) ◽  
pp. 1448-1455 ◽  
Author(s):  
Janine Schaeverbeke-Sacré ◽  
Béatrice Matheron
Keyword(s):  
Radiation Dose ◽  
Gamma Irradiation ◽  
Gamma Rays ◽  
Cytological Study ◽  
Jerusalem Artichoke ◽  
Dna And Rna ◽  
Jerusalem Artichoke Tuber ◽  
Outer Area

DNA and RNA contents are studied in Jerusalem artichoke tuber explants cultured in vitro after gamma irradiation (0–5 × 105 rads (1 rad = 10 mGy)). The lower part of the explants is stimulated as soon as in contact with the medium. This stimulated area is still able to synthesize DNA and RNA up to 104 rads. An histological and cytological study shows that tissue neoformations can be observed up to 6000 rads in this outer area and that gamma rays seem to keep the cells in a "premitotic" state for a longer or shorter period according to the applied radiation dose.

Effects of 2,4- and 3,5-dichlorophenoxyacetic acids on Jerusalem artichoke tuber tissue disks

1969 ◽  
Vol 8 (10) ◽  
pp. 1859-1866 ◽  
Author(s):  
P.P. Rutherford ◽  
E.W. Weston ◽  
A.E. Flood
Keyword(s):  
Jerusalem Artichoke ◽  
Tuber Tissue ◽  
Jerusalem Artichoke Tuber

Bilans nutritifs glucidique et azoté de tissus de tubercules de topinambour cultivés in vitro après irradiation gamma

1982 ◽  
Vol 60 (11) ◽  
pp. 2215-2218
Author(s):  
Janine Schaeverbeke-Sacré ◽  
Béatrice Matheron
Keyword(s):  
Amino Acid ◽  
Gamma Irradiation ◽  
Culture Medium ◽  
Reducing Sugars ◽  
Acid Leaching ◽  
Jerusalem Artichoke ◽  
Protein Nitrogen ◽  
Nitrogen Budgets ◽  
Jerusalem Artichoke Tuber

Carbohydrate and nitrogen budgets were studied in Jerusalem artichoke tuber explants cultured in vitro after gamma irradiation (0 to 106 rads (1 rad = 10−2 J/kg)). A certain level of reducing sugars is reached and retained in all the explants and, in all cases, an increase of protein nitrogen is observed. The highly irradiated tissues have a very disturbed metabolism that involves in particular, amino acid leaching into the culture medium.

β-1,3-Glucans may be associated with cell plate formation during cytokinesis

1976 ◽  
Vol 54 (5-6) ◽  
pp. 539-542 ◽  
Author(s):  
R. G. Fulcher ◽  
M. E. McCully ◽  
G. Setterfield ◽  
J. Sutherland
Keyword(s):  
Cell Walls ◽  
Lateral Root ◽  
Jerusalem Artichoke ◽  
Cell Plate ◽  
Rapid Freezing ◽  
Chemical Fixation ◽  
Cell Plate Formation ◽  
Jerusalem Artichoke Tuber ◽  
Plate Formation ◽  
Sieve Plates

Sections of young lateral-root meristems of corn seedlings and Jerusalem artichoke tuber slices treated to induce cell divisions were stained with aniline blue and examined by fluorescence microscopy. In both tissues intense yellow fluorescence, similar to that given by callose in sieve plates, was observed in cell plates of cells undergoing cytokinesis and in recently formed transverse cell walls of newly divided cells. Staining was found after both chemical fixation and rapid freezing and was removed by prior β-1,3-glucanase treatment. The staining was not present in older walls. The results suggest that a unique polysaccharide, probably a β-1,3-glucan, is associated with the early stages of cell wall formation in cytokinesis. The role and fate of the polysaccharide are unknown.

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