Disc electrophoresis of snake venoms. I. A qualitative comparison of the protein patterns from the species of Crotalidae and Elapidae

1969 ◽  
Vol 47 (8) ◽  
pp. 807-810 ◽  
Author(s):  
Arun S. Basu ◽  
Reno Parker ◽  
Rod O'Connor
Keyword(s):  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Snake Venoms ◽  
Protein Patterns ◽  
Qualitative Comparison ◽  
Venomous Snakes ◽  
Electrophoretic Patterns ◽  
Ph Conditions ◽  
Venom Proteins

The electrophoretic patterns of venom proteins of different species of snakes from the families of Crotalidae and Elapidae were studied by disc electrophoresis using different concentrations of polyacrylamide gel and different pH conditions. A qualitative comparison of the electropherograms showed variations in the distribution of proteins among different species and subspecies of snakes. It is suggested that the classification of venomous snakes from the standpoint of venom compositions is of fundamental importance in snakebite pathology.

SEED PROTEIN HOMOLOGIES AND THE EVOLUTION OF POLYPLOIDY IN AVENA

1972 ◽  
Vol 14 (4) ◽  
pp. 875-888 ◽  
Author(s):  
G. Ladizinsky ◽  
B. L. Johnson
Keyword(s):  
Optical Density ◽  
Seed Protein ◽  
Correlation Coefficients ◽  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Individual Species ◽  
Protein Patterns ◽  
Independent Origin ◽  
Pattern Variability ◽  
Tetraploid Parent

Crude seed protein of the nine oat species was fractionated by disc electrophoresis on polyacrylamide gel. Correlation coefficients calculated from optical density curves obtained from the stained gels showed that individual species possessed characteristic, albeit variable, profiles. Pattern variability among and within species was greater in the case of the diploids than in the case of the polyploids. The very few profile types in A. strigosa 4x and in the hexaploid A. sativa suggested that the variation in these polyploids is due more to independent origin of the types than to differentiation following polyploidization. Virtual identity between individual A. strigosa 2x and 4x profiles suggested a strict autopolyploid origin for some 4x types while complementing pairs of A. strigosa 2x profiles indicated an intervarietal origin for other A. strigosa 4x types. A diagnostic band at 11.0 cm in the profiles of A. magna and A. murphyi suggested that these species rather than A. strigosa 4x had functioned as the tetraploid parent of the hexaploid A. sativa. The profile of A. murphyi complemented by a specific A. strigosa 2x profile simulated a specific A. sativa type. The adaptive success of the genus is assessed in the light of variation and homologies in the seed protein patterns of the various species.

scholarly journals Classification of Lipoprotein Profile by Polyacrylamide Gel Disc Electrophoresis

2003 ◽  
Vol 42 (3) ◽  
pp. 244-249 ◽  
Author(s):  
Akihiro YOSHIDA ◽  
Michiteru KODAMA ◽  
Hideki NOMURA ◽  
Michitaka NAITO
Keyword(s):  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Lipoprotein Profile

Electrophoretic patterns of soluble fungal proteins and their possible use as taxonomic criteria in the genus Fusarium

1969 ◽  
Vol 47 (12) ◽  
pp. 1823-1831 ◽  
Author(s):  
A. N. Glynn ◽  
J. Reid
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Species ◽  
Soluble Proteins ◽  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Current Concept ◽  
Formae Speciales ◽  
Genus Fusarium ◽  
Electrophoretic Patterns ◽  
Taxonomic Criterion

Buffer-soluble proteins extracted from 33 isolates of various Fusarium oxysporum formae speciales and 13 other Fusarium species were separated on polyacrylamide gel disc electrophoresis. The resulting patterns were compared and their use as a taxonomic criterion assessed. The results obtained do not support the current concept that this method is useful as a taxonomic tool.

Electrophoretic patterns of globulins and albumins in conifer seeds

1970 ◽  
Vol 48 (11) ◽  
pp. 1911-1912 ◽  
Author(s):  
Pei-Show Juo ◽  
G. Stotzky
Keyword(s):  
Disc Electrophoresis ◽  
Protein Patterns ◽  
Electrophoretic Patterns ◽  
Conifer Seeds ◽  
Degree Of Similarity

The globulins and albumins in seeds of nine species of conifers were distinguished by disc electrophoresis. On the basis of their higher stain intensity, the slower moving proteins, located in the upper half of the separation gels, appeared to be dominant. Only one globulin and one albumin band were homologous in all species examined, although several globulin and albumin bands appeared to be common to many species. The degree of similarity in protein patterns between species classified within the same genus and the degree of difference between genera were not as great as those reported for seeds of some angiosperms.

The Effect of Antithrombin III on the Activity of the Coagulation Factors VII, IX and X

1976 ◽  
Vol 35 (02) ◽  
pp. 295-304 ◽  
Author(s):  
B Østerud ◽  
M Miller-Andersson ◽  
U Abildgaard ◽  
H Prydz
Keyword(s):  
Antithrombin Iii ◽  
Factor Xa ◽  
Coagulation Factors ◽  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Factor Vii ◽  
Native Form ◽  
Factor Ixa ◽  
Plasma Factor

SummaryAntithrombin III, purified to homogeneity according to Polyacrylamide gel disc electrophoresis and immunoelectrophoresis, inhibited the activity of purified factor IXa and Xa, whereas factor VII was not inhibited either in the active or in the native form.Antithrombin III is the single most important inhibitor of factor Xa in plasma. Factor Xa does not, however, reduce the activity of antithrombin III against thrombin.

IDENTIFICATION OF CULTIVARS OF FORAGE LEGUME (Desmodium ovalifolium GUILL ET PERR.) BY THEIR ELECTROPHORETIC PATTERNS

1987 ◽  
Vol 67 (3) ◽  
pp. 713-717 ◽  
Author(s):  
A. HUSSAIN ◽  
W. BUSHUK ◽  
H. RAMIREZ ◽  
W. ROCA
Keyword(s):  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Polyacrylamide Gel Electrophoresis ◽  
Seed Proteins ◽  
Polyacrylamide Gel ◽  
Forage Legume ◽  
Desmodium Ovalifolium ◽  
Electrophoretic Patterns ◽  
Electrophoretic Procedure

An electrophoretic procedure was developed for discriminating cultivars of Desmodium ovalifolium on the basis of patterns of partially purified seed proteins. Electrophoresis was done on uniform 15% polycrylamide gels in basic (8.9) pH. The method produced satisfactory discrimination of eight cultivars used in its initial evaluation.Key words: Forage legume, Desmodium ovalifolium Guill et Perr., cultivar identification, polyacrylamide gel electrophoresis

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