Electrophoretic patterns of globulins and albumins in conifer seeds

1970 ◽  
Vol 48 (11) ◽  
pp. 1911-1912 ◽  
Author(s):  
Pei-Show Juo ◽  
G. Stotzky
Keyword(s):  
Disc Electrophoresis ◽  
Protein Patterns ◽  
Electrophoretic Patterns ◽  
Conifer Seeds ◽  
Degree Of Similarity

The globulins and albumins in seeds of nine species of conifers were distinguished by disc electrophoresis. On the basis of their higher stain intensity, the slower moving proteins, located in the upper half of the separation gels, appeared to be dominant. Only one globulin and one albumin band were homologous in all species examined, although several globulin and albumin bands appeared to be common to many species. The degree of similarity in protein patterns between species classified within the same genus and the degree of difference between genera were not as great as those reported for seeds of some angiosperms.

Disc electrophoresis of snake venoms. I. A qualitative comparison of the protein patterns from the species of Crotalidae and Elapidae

1969 ◽  
Vol 47 (8) ◽  
pp. 807-810 ◽  
Author(s):  
Arun S. Basu ◽  
Reno Parker ◽  
Rod O'Connor
Keyword(s):  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Snake Venoms ◽  
Protein Patterns ◽  
Qualitative Comparison ◽  
Venomous Snakes ◽  
Electrophoretic Patterns ◽  
Ph Conditions ◽  
Venom Proteins

The electrophoretic patterns of venom proteins of different species of snakes from the families of Crotalidae and Elapidae were studied by disc electrophoresis using different concentrations of polyacrylamide gel and different pH conditions. A qualitative comparison of the electropherograms showed variations in the distribution of proteins among different species and subspecies of snakes. It is suggested that the classification of venomous snakes from the standpoint of venom compositions is of fundamental importance in snakebite pathology.

Electrophoretic Patterns of Soluble Proteins and Isoenzymes of Gaeumannomyces graminis

1975 ◽  
Vol 23 (1) ◽  
pp. 1 ◽  
Author(s):  
LK Abbott ◽  
AA Holland
Keyword(s):  
Isoelectric Focusing ◽  
Gel Electrophoresis ◽  
Morphological Characters ◽  
Disc Electrophoresis ◽  
Gaeumannomyces Graminis ◽  
Protein Patterns ◽  
Peroxidase Isoenzyme ◽  
Gradient Gel Electrophoresis ◽  
Kikuyu Grass ◽  
Take All

The wheat and oat take-all fungi show a close serological relationship with Gaeumannomyces graminis var. graminis isolated from kikuyu grass, supporting the conclusions of Walker, who recognized the three fungal groups as varieties of G. graminis based on morphological characters. Three techniques for studying proteins (disc and gradient gel electrophoresis and isoelectric focusing) were assessed as possible aids in identifying and classifying isolates of G. graminis. Protein patterns were affected by the age of the mycelium and the composition of the growth medium used. Patterns prepared by disc electrophoresis were helpful for identifying isolates of G. graminis, but not for separating the varieties. Gradient gel electrophoresis patterns were sufficient for distin- guishing the kikuyu grass isolate from take-all fungi isolated from wheat or oats, but the differences between the patterns of the wheat and oat take-all fungi were too slight to aid in identifying these varieties. Esterase and peroxidase isoenzyme patterns of the isolates from the three varieties were too variable to assist in identifying isolates of G. graminis at either the species or the variety level. Isoelectric focusing patterns of the proteins for each variety were useful for distinguishing the kikuyu grass isolate from the wheat and oat take-all fungi. The protein patterns illustrate a closer relationship between the wheat and oat take-all fungi than either has with the variety of G. graminis from kikuyu grass. None of the methods gave a convenient means of separating the wheat and oat take-all fungi.

Quantitative Electrophoresis of Serum Proteins on Paper

1956 ◽  
Vol 2 (5) ◽  
pp. 303-319 ◽  
Author(s):  
Moses Wurm ◽  
Frederick H Epstein
Keyword(s):  
Protein Concentration ◽  
Moving Boundary ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Bromphenol Blue ◽  
Good Resolution ◽  
Confidence Limits ◽  
Protein Patterns ◽  
Electrophoretic Patterns ◽  
Normal Human

Abstract 1. A procedure for paper electrophoresis has been described which gives highly reproducible protein patterns with good resolution and freedom from distortions. 2. Densitometry of protein bands on paper stained with bromphenol blue or Amidoschwarz 10B reveals that the logarithm of protein concentration is proportional to optical density and that Beer's law does not apply. Electrophoretic patterns of normal human serum evaluated in this manner give values in close agreement with those obtained by moving-boundary electrophoresis. 3. Confidence limits were determined for both methods.

scholarly journals Variety-specificity of soluble proteins of potato tubers

2015 ◽  
Vol 43 (4) ◽  
pp. 491-498 ◽  
Author(s):  
Marta Czupryn ◽  
Kazimierz Toczko
Keyword(s):  
Potato Tuber ◽  
Genetic Research ◽  
Electrophoretic Pattern ◽  
Electrophoretic Analysis ◽  
Disc Electrophoresis ◽  
The Other ◽  
Potato Tubers ◽  
Genetic Origin ◽  
Electrophoretic Patterns ◽  
Taxonomic Studies

Separation of soluble tuber proteins from six potato clones and twelve varieties cultivated in Poland has been accomplished by disc electrophoresis. It was found that electrophoretic pattern was unique for a given clone or variety. Data obtained confirm results of the other authors for the other varieties and indicate that electrophoretic analysis of potato tuber proteins can be a useful method for taxonomic studies. Such analysis however cannot be used for genetic research since no correlations were found between electrophoretic patterns and genetic origin of respective clones and varieties.

scholarly journals Metabolism of excised embryos of Lupinus luteus L. VI. An electrophoretic analysis of some dehydrogenases in cultured embryos as compared with the normal seedling axes

2015 ◽  
Vol 43 (1) ◽  
pp. 117-127 ◽  
Author(s):  
J. Czosnowski
Keyword(s):  
Lactate Dehydrogenase ◽  
Glutamate Dehydrogenase ◽  
Electrophoretic Analysis ◽  
Disc Electrophoresis ◽  
Lupinus Luteus ◽  
The Third ◽  
Electrophoretic Patterns ◽  
Normal Seedling ◽  
Axial Organs ◽  
Excised Embryos

The electrophoretic patterns (disc electrophoresis) of the studied dehydrogenases: glucose-6-phosphate - (A), malate - (B), glutamate - (C), alcohol - (D) and lactate dehydrogenase (E), in the axial organs of isolated <i>Lupinus luteus</i> embryos and seedlings cultivated over 12 days are characterized by great similarities. With time, after the third day of cultivation the patterns begin to become less deyeloped. Analyses performed during the first 10 hours of imbibition of seed parts indicate that the maximal development of isozyme patterns occurs during the third hour after which the patterns become poorer. The most uniform type of pattern. and the lowest number of isozymes was shown by glutamate dehydrogenase, the richest pattern was shown by malate dehydrogenase. No band common for a 11 the 27 experimental elements was found.

scholarly journals Heterogeneity of the acid phosphatase and ribonuclease from protocorms of the orchids Cymbidium Sw. and changes occurring after treatment with streptomycin

2015 ◽  
Vol 42 (1) ◽  
pp. 133-141 ◽  
Author(s):  
B. Morawiecka ◽  
A. Kubicz ◽  
K. Kukułczanka ◽  
A. Koch ◽  
E. Markefka
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Culture Medium ◽  
Disc Electrophoresis ◽  
Molecular Forms ◽  
Stages Of Development ◽  
Early Stages ◽  
Electrophoretic Patterns ◽  
Culture Growth

The molecular forms of the acid phosphatase and RNase in protocorms of <i>Cymbidium</i> Sw. were studied by disc electrophoresis. The effect of streptomycin added to the culture medium on both enzymes was investigated. Significant changes in enzyme activity and electrophoretic patterns occured after addition of streptomycin at the beginning of culture growth. This indicates that the enzymes are affected by streptomycin in early stages of development of the protocorms.

Control of protein synthesis during the development of Acetabularia

Development ◽  
1971 ◽  
Vol 26 (2) ◽  
pp. 323-338
Author(s):  
Gabriel Ceron ◽  
E. Marshall Johnson
Keyword(s):  
Protein Synthesis ◽  
Membrane Fraction ◽  
Developmental Stages ◽  
Soluble Fraction ◽  
Protein Species ◽  
General Phenomenon ◽  
Protein Patterns ◽  
Electrophoretic Patterns ◽  
Zone Electrophoresis ◽  
Autoradiographic Analysis

Proteins from the soluble, chloroplastic and cell membrane fractions of axenically grown Acetabularia were analysed by zone electrophoresis. Incorporation of [14C]leucine into different proteins was measured by autoradiographic analysis of the electrophoretic patterns. The protein patterns from the soluble fraction remain constant with respect to the number of detectable bands but change with respect to the relative synthetic rates at various developmental stages. The protein patterns from the membrane fraction change with respect to both the number of protein species and the relative synthetic rates. The analysis of the synthetic performance of enucleated cells revealed that most of the proteins from the soluble and the membrane fractions continue to be synthesized in the absence of the nucleus and that the changes that normally occur in the protein patterns of the membrane fraction at the time of cap formation also take place in enucleated cells. This is taken as an indication that the control of the synthesis of the proteins studied is of extranuclear nature. It was also found that chloroplasts are capable of synthesizing all the components of the chloroplastic protein spectrum at least 4 weeks after enucleation. Some of the chloroplastic proteins can also be synthesized by purified chloroplasts in extracellular conditions. The possibility of extranuclear control of protein synthesis being a rather general phenomenon during the development of Acetabularia is discussed.

An electrophoretic comparison of the venoms of a colubrid and various viperid snakes from Turkey and Cyprus, with some taxonomic and phylogenetic implications

Zootaxa ◽  
2005 ◽  
Vol 1038 (1) ◽  
pp. 1 ◽  
Author(s):  
HÜSEYIN ARIKAN ◽  
BAYRAM GÖÇMEN ◽  
AHMET MERMER ◽  
HASAN BAHAR
Keyword(s):  
Total Protein ◽  
Disc Electrophoresis ◽  
Venom Protein ◽  
Electrophoretic Patterns ◽  
Phylogenetic Implications ◽  
The Family ◽  
Electrophoretic Data ◽  
Densitometry Analysis ◽  
Macrovipera Lebetina Obtusa ◽  
Vipera Ammodytes

Venom extracts obtained from a colubrid snake [Malpolon monspessulanus (Hermann)] and eight viperids [Vipera xanthina (Gray), Vipera ammodytes (Linnaeus), Vipera kaznakovi (Nikolsky), Vipera eriwanensis (Bonaparte), Vipera wagneri Nilson &Andrén, Vipera barani Böhme & Joger, Macrovipera lebetina lebetina (Linnaeus), Macrovipera lebetina obtusa Dwigubsky] distributed in Turkey and Cyprus were compared using polyacrylamide gel disc electrophoresis and densitometry analysis methods. The electrophoretic patterns of the examined snakes were demonstrated. The obtained electropherograms of the examined venom protein samples showed important qualitative differences between the colubrid snake, Malpolon monspessulanus and the viperid snakes; in the colubrid sample the total protein fraction number was 8, while in viperid samples they number between 10 and 14, indicating the venom complexity in viperids is higher than that of colubrid snakes. Electrophoretic data support the phylogenetic argument previously outlined of the family Viperidae. Moreover, in the light of the differences of the venom electrophoretic patterns, it is concluded that the southern Anatolian population of Macrovipera lebetina should not be identified as the nominate subspecies M. l. lebetina, which lives in Cyprus.

SOLUBLE PROTEINS AND COLD HARDINESS OF TWO WOODY SPECIES

1969 ◽  
Vol 49 (3) ◽  
pp. 279-286 ◽  
Author(s):  
L. E. Craker ◽  
L. V. Gusta ◽  
C. J. Weiser
Keyword(s):  
Low Temperature ◽  
Moisture Content ◽  
Total Nitrogen ◽  
Cold Hardiness ◽  
Morphological Changes ◽  
Woody Species ◽  
Soluble Proteins ◽  
Disc Electrophoresis ◽  
Ionic Surfactant ◽  
Electrophoretic Patterns

A simplified, highly reproducible procedure is outlined for the extraction and polyacrylamide gel disc electrophoresis of acidic soluble proteins from apple bark and arborvitae foliage. The procedure includes low temperature maceration, short extraction time, and an extraction solution which contains polyol and phenolic complexers, a reducing agent, and a non-ionic surfactant. Electrophoretic patterns, total nitrogen, moisture content, minimum survival temperatures, and environmental and morphological changes were examined during the natural dehardening of apple and the controlled hardening of arborvitae. Qualitative protein changes, as evidenced by the appearance and disappearance of specific bands, occurred at times when changes in hardiness were taking place.

Sign in / Sign up

Export Citation Format

Share Document