SEED PROTEIN HOMOLOGIES AND THE EVOLUTION OF POLYPLOIDY IN AVENA

1972 ◽  
Vol 14 (4) ◽  
pp. 875-888 ◽  
Author(s):  
G. Ladizinsky ◽  
B. L. Johnson
Keyword(s):  
Optical Density ◽  
Seed Protein ◽  
Correlation Coefficients ◽  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Individual Species ◽  
Protein Patterns ◽  
Independent Origin ◽  
Pattern Variability ◽  
Tetraploid Parent

Crude seed protein of the nine oat species was fractionated by disc electrophoresis on polyacrylamide gel. Correlation coefficients calculated from optical density curves obtained from the stained gels showed that individual species possessed characteristic, albeit variable, profiles. Pattern variability among and within species was greater in the case of the diploids than in the case of the polyploids. The very few profile types in A. strigosa 4x and in the hexaploid A. sativa suggested that the variation in these polyploids is due more to independent origin of the types than to differentiation following polyploidization. Virtual identity between individual A. strigosa 2x and 4x profiles suggested a strict autopolyploid origin for some 4x types while complementing pairs of A. strigosa 2x profiles indicated an intervarietal origin for other A. strigosa 4x types. A diagnostic band at 11.0 cm in the profiles of A. magna and A. murphyi suggested that these species rather than A. strigosa 4x had functioned as the tetraploid parent of the hexaploid A. sativa. The profile of A. murphyi complemented by a specific A. strigosa 2x profile simulated a specific A. sativa type. The adaptive success of the genus is assessed in the light of variation and homologies in the seed protein patterns of the various species.

The comparison of seed protein patterns within the genusArachis by polyacrylamide gel electrophoresis

1983 ◽  
Vol 25 (4) ◽  
pp. 266-273 ◽  
Author(s):  
Eva Klozová ◽  
Jiřina Švachulová ◽  
J. Smartt ◽  
E. Hadač ◽  
Věra Turková ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Seed Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Protein Patterns

Disc electrophoresis of snake venoms. I. A qualitative comparison of the protein patterns from the species of Crotalidae and Elapidae

1969 ◽  
Vol 47 (8) ◽  
pp. 807-810 ◽  
Author(s):  
Arun S. Basu ◽  
Reno Parker ◽  
Rod O'Connor
Keyword(s):  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Snake Venoms ◽  
Protein Patterns ◽  
Qualitative Comparison ◽  
Venomous Snakes ◽  
Electrophoretic Patterns ◽  
Ph Conditions ◽  
Venom Proteins

The electrophoretic patterns of venom proteins of different species of snakes from the families of Crotalidae and Elapidae were studied by disc electrophoresis using different concentrations of polyacrylamide gel and different pH conditions. A qualitative comparison of the electropherograms showed variations in the distribution of proteins among different species and subspecies of snakes. It is suggested that the classification of venomous snakes from the standpoint of venom compositions is of fundamental importance in snakebite pathology.

The Effect of Antithrombin III on the Activity of the Coagulation Factors VII, IX and X

1976 ◽  
Vol 35 (02) ◽  
pp. 295-304 ◽  
Author(s):  
B Østerud ◽  
M Miller-Andersson ◽  
U Abildgaard ◽  
H Prydz
Keyword(s):  
Antithrombin Iii ◽  
Factor Xa ◽  
Coagulation Factors ◽  
Disc Electrophoresis ◽  
Polyacrylamide Gel ◽  
Factor Vii ◽  
Native Form ◽  
Factor Ixa ◽  
Plasma Factor

SummaryAntithrombin III, purified to homogeneity according to Polyacrylamide gel disc electrophoresis and immunoelectrophoresis, inhibited the activity of purified factor IXa and Xa, whereas factor VII was not inhibited either in the active or in the native form.Antithrombin III is the single most important inhibitor of factor Xa in plasma. Factor Xa does not, however, reduce the activity of antithrombin III against thrombin.

Fibrinogen Montreal

1973 ◽  
Vol 29 (03) ◽  
pp. 536-546 ◽  
Author(s):  
M Lacombe ◽  
J Soria ◽  
C Soria ◽  
G d’Angelo ◽  
R Lavallee ◽  
...  
Keyword(s):  
Optical Density ◽  
Polyacrylamide Gel ◽  
Clotting Time ◽  
Functional Defect ◽  
A Chain ◽  
Fibrin Monomers

SummaryA new case of congenital dysfibrinogenemia characterized by a prolonged thrombin clotting time and a low optical density of the polymerization curve has been discovered in Montreal. The functional defect is due to an abnormal aggregation of fibrin monomers.The characteristics of this abnormal fibrinogen are serum gélification (Paracoagulation) at 37°, 22° and 4° C, a normal immuno-electrophoretic and electrofocusing pattern, a slight increase in the mobility in the α (A) chain by electrophoresis of the dissociated chains in polyacrylamide gel. However, no abnormality was found in the α (A) chain of the disulphide knot.

scholarly journals Simultaneous absolute quantification and sequencing of fish environmental DNA in a mesocosm by quantitative sequencing technique

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tatsuhiko Hoshino ◽  
Ryohei Nakao ◽  
Hideyuki Doi ◽  
Toshifumi Minamoto
Keyword(s):  
Fish Species ◽  
High Throughput Sequencing ◽  
Correlation Coefficients ◽  
Environmental Dna ◽  
Digital Pcr ◽  
Absolute Quantification ◽  
Taqman Probe ◽  
Individual Species ◽  
Natural Environments ◽  
Target Sequence

AbstractThe combination of high-throughput sequencing technology and environmental DNA (eDNA) analysis has the potential to be a powerful tool for comprehensive, non-invasive monitoring of species in the environment. To understand the correlation between the abundance of eDNA and that of species in natural environments, we have to obtain quantitative eDNA data, usually via individual assays for each species. The recently developed quantitative sequencing (qSeq) technique enables simultaneous phylogenetic identification and quantification of individual species by counting random tags added to the 5′ end of the target sequence during the first DNA synthesis. Here, we applied qSeq to eDNA analysis to test its effectiveness in biodiversity monitoring. eDNA was extracted from water samples taken over 4 days from aquaria containing five fish species (Hemigrammocypris neglectus, Candidia temminckii, Oryzias latipes, Rhinogobius flumineus, and Misgurnus anguillicaudatus), and quantified by qSeq and microfluidic digital PCR (dPCR) using a TaqMan probe. The eDNA abundance quantified by qSeq was consistent with that quantified by dPCR for each fish species at each sampling time. The correlation coefficients between qSeq and dPCR were 0.643, 0.859, and 0.786 for H. neglectus, O. latipes, and M. anguillicaudatus, respectively, indicating that qSeq accurately quantifies fish eDNA.

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