FRACTIONATION AND COMPOSITION OF RIBONUCLEIC ACIDS FROM SCHIZOSACCHAROMYCES POMBE

1967 ◽  
Vol 45 (6) ◽  
pp. 973-978 ◽  
Author(s):  
E. Katona
Keyword(s):  
Schizosaccharomyces Pombe ◽  
Protein Content ◽  
Base Composition ◽  
P Values ◽  
Ribonucleic Acids ◽  
Analytical Results

A slightly modified variation of the Crestfield detergent method was used for isolation of the ribonucleic acids from Schizosaccharomyces pombe. A detailed description of the method is given. Nine fractions were prepared and analyzed for RNA, DNA, and protein content. These analytical results, together with yields, ε(P) values, and detailed results of the base composition of the fractions are presented.

scholarly journals EXPERIMENTALLY INDUCED CHANGES IN THE BASE COMPOSITION OF THE RIBONUCLEIC ACIDS OF ISOLATED NERVE CELLS AND THEIR OLIGODENDROGLIAL CELLS

1961 ◽  
Vol 10 (3) ◽  
pp. 403-410 ◽  
Author(s):  
Endre Egyházi ◽  
Holger Hydén
Keyword(s):  
Protein Content ◽  
Nerve Cell ◽  
Glial Cells ◽  
Base Composition ◽  
Functional Relationship ◽  
Nerve Cells ◽  
Ribonucleic Acids ◽  
Cent Increase ◽  
The Difference ◽  
Induced Changes

The effect of tricyano-amino-propene, a dimer of malononitrile, on the base composition of the RNA in isolated Deiters' nerve cells and their oligodendroglial cells has been studied using a microelectrophoretic method. Tri-a-p in a dose of 20 mg/kg has the effect of increasing the RNA and protein content per nerve cell by 25 per cent and decreasing the glia RNA by 45 per cent. The RNA base composition of the nerve cells from the control animals differs from that of their glial cells. The guanine of the nerve cell is significantly higher than that of the glia, but the content of cytosine is higher in the glia than in the RNA of nerve cell. The cytosine of nerve cells decreased significantly after tri-a-p administration. In the glial cells the cytosine showed a 20 per cent increase, and the guanine a 25 per cent decrease. Tri-a-p sharpened the difference in RNA composition already existing between the control nerve cells and their glial cells by almost 300 per cent for the guanine and by 400 per cent for the cytosine. The chemical and functional relationship between the nerve cell and its oligodendroglial cells is discussed.

scholarly journals Usefulness of the prep statistic as an alternative to null-hypotheses significance testing using p values: Comparisons of analytical results based on prep with p

2008 ◽  
Vol 6 (2) ◽  
pp. 23-26
Author(s):  
Manabu Ichihara ◽  
Tomoko Sugimura ◽  
Yasunao Otsubo ◽  
Masayuki Kurokawa ◽  
Ikuo Sasayama ◽  
...  
Keyword(s):  
Significance Testing ◽  
P Values ◽  
Analytical Results

The base composition of ribonucleic acids from alfalfa mosaic virus components

Virology ◽  
1964 ◽  
Vol 23 (2) ◽  
pp. 283-286 ◽  
Author(s):  
A.G. Rauws ◽  
E.M.J. Jaspars ◽  
H. Veldstra
Keyword(s):  
Mosaic Virus ◽  
Base Composition ◽  
Alfalfa Mosaic Virus ◽  
Ribonucleic Acids

De novo biosynthesis and base composition of total and ribosomal ribonucleic acids during early development in Artemia sp.

FEBS Letters ◽  
1989 ◽  
Vol 255 (2) ◽  
pp. 358-360 ◽  
Author(s):  
Antonio Liras ◽  
M.C.Hernández Cruz ◽  
Pedro Rotllán ◽  
Luisa Argomaniz ◽  
Pilar Llorente
Keyword(s):  
Early Development ◽  
Base Composition ◽  
De Novo ◽  
Ribonucleic Acids ◽  
De Novo Biosynthesis

Protein synthesis and its relation to the DNA-division cycle in the fission yeast Schizosaccharomyces pombe

1984 ◽  
Vol 69 (1) ◽  
pp. 199-210
Author(s):  
J. Creanor ◽  
J.M. Mitchison
Keyword(s):  
Cell Cycle ◽  
Protein Synthesis ◽  
Schizosaccharomyces Pombe ◽  
Protein Content ◽  
Fission Yeast ◽  
Nuclear Division ◽  
Restrictive Temperature ◽  
Tentative Conclusion ◽  
Synchronous Cultures

The rate of protein synthesis has been measured with pulse labels of [3H]tryptophan in synchronous and asynchronous cultures of cdc mutants of Schizosaccharomyces pombe shifted up to the restrictive temperature. The cell cycle related fluctuations in rate that occur in normal synchronous cultures vanish when nuclear division is blocked in synchronous cultures of cdc2 and cdc10. But they persist in cdc11 where nuclear division continues and cleavage is stopped. We conclude that nuclear division affects the rate of synthesis and that this effect is inhibitory and probably persists for the last 40% of the cycle. When nuclear division has been blocked, the rate of synthesis continues to increase until a plateau is reached where the rate remains constant. Three size mutants of cdc2 reach the plateau at the same average protein content per cell although their initial protein contents vary over a threefold range. Comparison of these results with those from cdc10 leads to the tentative conclusion that the plateau starts when the cells reach a critical protein/DNA ratio.

The purification and properties of valine tRNAs of Drosophila melanogaster

1978 ◽  
Vol 56 (6) ◽  
pp. 618-623 ◽  
Author(s):  
Robert Dunn ◽  
W. R. Addison ◽  
I. C. Gillam ◽  
G. M. Tener
Keyword(s):  
Drosophila Melanogaster ◽  
Column Chromatography ◽  
Base Composition ◽  
The Other ◽  
Ribonucleic Acids ◽  
Purification And Properties ◽  
Major Species

The valine transfer ribonucleic acids of Drosophila melanogaster have been purified by column chromatography on BD-cellulose, Sepharose 6B, and RPC-5. Three major species were analyzed for base composition and coding properties. [Formula: see text] binds strongly to ribosomes in the presence of GUA and to a lesser extent with GUU and GUG. [Formula: see text] binds strongly in the presence of GUG and very poorly if at all with the other three triplets whereas [Formula: see text], which contains inosine, binds strongly in the presence of GUU, GUC, and GUA and weakly with GUG.

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