The purification and properties of valine tRNAs of Drosophila melanogaster

1978 ◽  
Vol 56 (6) ◽  
pp. 618-623 ◽  
Author(s):  
Robert Dunn ◽  
W. R. Addison ◽  
I. C. Gillam ◽  
G. M. Tener
Keyword(s):  
Drosophila Melanogaster ◽  
Column Chromatography ◽  
Base Composition ◽  
The Other ◽  
Ribonucleic Acids ◽  
Purification And Properties ◽  
Major Species

The valine transfer ribonucleic acids of Drosophila melanogaster have been purified by column chromatography on BD-cellulose, Sepharose 6B, and RPC-5. Three major species were analyzed for base composition and coding properties. [Formula: see text] binds strongly to ribosomes in the presence of GUA and to a lesser extent with GUU and GUG. [Formula: see text] binds strongly in the presence of GUG and very poorly if at all with the other three triplets whereas [Formula: see text], which contains inosine, binds strongly in the presence of GUU, GUC, and GUA and weakly with GUG.

scholarly journals Transvection at the End of the Truncated Chromosome in Drosophila melanogaster

Genetics ◽  
2003 ◽  
Vol 163 (4) ◽  
pp. 1375-1387
Author(s):  
Mikhail Savitsky ◽  
Tatyana Kahn ◽  
Ekaterina Pomerantseva ◽  
Pavel Georgiev
Keyword(s):  
Drosophila Melanogaster ◽  
Homologous Chromosome ◽  
Regulatory Region ◽  
Proximal Region ◽  
The Other ◽  
Upstream Region ◽  
Upstream Sequence ◽  
Transcription Start ◽  
Promoter Proximal Region

Abstract The phenomenon of transvection is well known for the Drosophila yellow locus. Thus enhancers of a promoterless yellow locus in one homologous chromosome can activate the yellow promoter in the other chromosome where the enhancers are inactive or deleted. In this report, we examined the requirements for trans-activation of the yellow promoter at the end of the deficient chromosome. A number of truncated chromosomes ending in different areas of the yellow regulatory region were examined in combination with the promoterless y alleles. We found that trans-activation of the yellow promoter at the end of a deficient chromosome required ∼6 kb of an additional upstream sequence. The nature of upstream sequences affected the strength of transvection: addition of gypsy sequences induced stronger trans-activation than addition of HeT-A or yellow sequences. Only the promoter proximal region (within -158 bp of the yellow transcription start) was essential for trans-activation; i.e., transvection did not require extensive homology in the yellow upstream region. Finally, the yellow enhancers located on the two pairing chromosomes could cooperatively activate one yellow promoter.

scholarly journals Genetics of Differences in Pheromonal Hydrocarbons Between Drosophila melanogaster and D. simulans

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 353-364 ◽  
Author(s):  
Jerry A Coyne
Keyword(s):  
Drosophila Melanogaster ◽  
Sexual Dimorphism ◽  
Genetic Analysis ◽  
Species Difference ◽  
The Other ◽  
Chromosome 3 ◽  
Apparent Effect ◽  
The Third ◽  
Hydrocarbon Profile ◽  
The Right

Abstract Females of Drosophila melanogaster and its sibling species D. simulans have very different cuticular hydrocarbons, with the former bearing predominantly 7,11-heptacosadiene and the latter 7-tricosene. This difference contributes to reproductive isolation between the species. Genetic analysis shows that this difference maps to only the third chromosome, with the other three chromosomes having no apparent effect. The D. simulans alleles on the left arm of chromosome 3 are largely recessive, allowing us to search for the relevant regions using D. melanogaster deficiencies. At least four nonoverlapping regions of this arm have large effects on the hydrocarbon profile, implying that several genes on this arm are responsible for the species difference. Because the right arm of chromosome 3 also affects the hydrocarbon profile, a minimum of five genes appear to be involved. The large effect of the third chromosome on hydrocarbons has also been reported in the hybridization between D. simulans and its closer relative D. sechellia, implying either an evolutionaly convergence or the retention in D. sechllia of an ancestral sexual dimorphism.

scholarly journals Rex-induced recombination implies bipolar organization of the ribosomal RNA genes of Drosophila melanogaster.

Genetics ◽  
1988 ◽  
Vol 120 (4) ◽  
pp. 1053-1059
Author(s):  
L G Robbins ◽  
E E Swanson
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Mitotic Recombination ◽  
Ribosomal Rna Genes ◽  
The Other ◽  
Other Orientation ◽  
Rna Genes ◽  
Nucleolus Organizers

Abstract Rex-induced mitotic recombination was used to determine whether nucleolus organizers can pair in both inverted and noninverted orientations. Two target chromosomes, each duplicated for the rDNA region, were exposed to maternal Rex activity. Recombination in one orientation should yield deletion of the material between the two nucleolus organizers, recombination in the other orientation should yield inversion of the same material. Both products were recovered from both target chromosomes. The generality of using Rex-mediated recombination for analysis of the rDNA is considered.

scholarly journals MULTIPLE MEIOTIC DRIVE SYSTEMS IN THE DROSOPHILA MELANOGASTER MALE

Genetics ◽  
1972 ◽  
Vol 72 (1) ◽  
pp. 105-115
Author(s):  
George L Gabor Miklos ◽  
Armon F Yanders ◽  
W J Peacock
Keyword(s):  
Drosophila Melanogaster ◽  
Survival Probability ◽  
Sex Chromosome ◽  
Meiotic Drive ◽  
The Other ◽  
Segregation Distorter ◽  
Two Systems ◽  
Combined Action ◽  
Drive Systems

ABSTRACT The behaviour of two "meiotic drive" systems, Segregation-Distorter (SD) and the sex chromosome sc4sc8 has been examined in the same meiocyte. It has been found that the two systems interact in a specific way. When the distorting effects of SD and sc4sc8 are against each other, there is no detectable interaction. Each system is apparently oblivious to the presence of the other, gametes being produced according to independence expectations. However when the affected chromosomes are at the same meiotic pole an interaction occurs; the survival probability of the gamete containing both distorted chromosomal products is increased, rather than being decreased by the combined action of two systems.

scholarly journals Inhibition and complexation of activated protein C by two major inhibitors in plasma

Blood ◽  
1989 ◽  
Vol 73 (2) ◽  
pp. 446-454 ◽  
Author(s):  
MJ Heeb ◽  
F Espana ◽  
JH Griffin
Keyword(s):  
Molecular Weight ◽  
Column Chromatography ◽  
Protein C ◽  
Activated Protein C ◽  
Plasma Formation ◽  
The Other ◽  
Amidolytic Activity

Abstract To determine the major physiologic inhibitors of activated protein C (APC), plasma was incubated with APC or with Protac C and subjected to immunoblotting. APC:inhibitor complexes gave two major bands reacting with antiprotein C antibodies when immunoblotted on nondenaturing gels, and additional minor bands that varied between serum and plasma. Formation of one of the two major bands of APC:inhibitor complex, but not the other, was stimulated by heparin and only this band reacted with antibodies to the previously described APC inhibitor that is here designated PCI-1. Plasma immunodepleted of PCI-1 formed complexes with APC as visualized with antiprotein C but not anti-PCI-1 antibodies, and exhibited heparin-independent inhibition of APC activity, providing evidence for the existence of a second major physiologic APC inhibitor, PCI-2. Formation of APC:PCI-2 complexes in PCI-1-depleted plasma paralleled inhibition of APC amidolytic activity. PCI-2 was separated from PCI-1 and partially purified using column chromatography. PCI-2 formed inactive complexes of approximately 110,000 molecular weight (mol wt) with APC suggesting PCI-2 has an approximate mol wt of 50,000. Thus, inhibition of APC in plasma involves two major distinct 50,000 mol wt inhibitors, the heparin-dependent PCI-1 and the heparin- independent PCI-2.

UV Determination of Sulfoxide in Formulations

1965 ◽  
Vol 48 (3) ◽  
pp. 569-573
Author(s):  
J B Haus ◽  
L Manolev ◽  
R W Price
Keyword(s):  
Silicic Acid ◽  
Column Chromatography ◽  
The Other ◽  
Ultraviolet Spectrophotometry

Abstract A method for the assay of sulfoxide in insectieidal formulations involves separation of the sulfoxide from the other components by column chromatography on silicic acid with three successive eluting solutions: chloroform; 2% acetone in chloroform; and 10% acetone in chloroform. Sulfoxide is removed in the last eluate and is determined by ultraviolet spectrophotometry. Solvents, emulsifiers, pyrethrins, compounds related to sulfoxide, and other insecticides tested are separated by the column.

scholarly journals Purification and properties of a new testosterone 17β-dehydrogenase (NADP+) from guinea-pig liver

1977 ◽  
Vol 163 (3) ◽  
pp. 401-407 ◽  
Author(s):  
E Kaguera ◽  
S Toki
Keyword(s):  
Guinea Pig ◽  
Column Chromatography ◽  
Gel Filtration ◽  
Deae Cellulose ◽  
Supernatant Fraction ◽  
Pig Liver ◽  
Ring Junction ◽  
Purification And Properties ◽  
Androstane Derivatives ◽  
Guinea Pig Liver

As a result of studies of guinea-pig live testosterone 17beta-dehydrogenase (NADP+) (EC 1.1.1.64), a new testosterone 17beta-dehydrogenase was discovered. The new enzyme was purified to a single homogeneous protein from the 105 000 g-supernatant fraction of guinea-pig liver by (NH4)2SO4 fractional precipitation and two gel-filtration stages, DEAE-cellulose column chromatography and hydroxyapatite column chromatography. It was characterized by many properties. The enzyme has almost the same properties as the classical testosterone 17beta-dehydrogenase (NADP+) (EC 1.1.1.64), with respect to cofactor requirement, pH optima for dehydrogenation, effect of phosphate ion on the NAD+-dependent reaction and molecular weight, but characteristic differences were observed in substrate-specificity between the two dehydrogenases. With various androstane derivatives, the configuration of the A/B-ring junction was closely connected with enzyme activity. 5alpha-Androstanes, such as 5alpha-androstane-3alpha,17beta-diol, 5alpha-androstane-3beta,17beta-diol and 17beta-hydroxy-5alpha-androstan-3-one, and 5beta-congeners, such as 5beta-androstane-3alpha,17beta-diol, 5beta-androstane-3beta,17beta-diol and 17beta-hydroxy-5beta-androstan-3-one, served as substrates for both the EC 1.1.1.64 enzyme and the new enzyme. The EC 1.1.1.64 enzyme oxidized testosterone more rapidly than did the new enzyme. These comparisons were based on the relative activities, apparent Km values and apparent Vmax values.

Resolution of ribonucleic acids by Sepharose 4B column chromatography

Biochemistry ◽  
1977 ◽  
Vol 16 (7) ◽  
pp. 1378-1382 ◽  
Author(s):  
Margarita Zeichner ◽  
Robert Stern
Keyword(s):  
Column Chromatography ◽  
Ribonucleic Acids ◽  
Sepharose 4B
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