INCORPORATION OF 14C INTO TWO PROTEINS FROM RAPESEED (BRASSICA NAPUS L.)

1966 ◽  
Vol 44 (7) ◽  
pp. 997-1004 ◽  
Author(s):  
A. J. Finlayson
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Brassica Napus ◽  
Sodium Acetate ◽  
Water Extract ◽  
Seed Meal ◽  
Brassica Napus L ◽  
Specific Activities ◽  
Considerable Period

Four groups of five rapeseed plants (Brassica napus L.) were injected with sodium acetate-2-14C at intervals between flowering and maturity. Two 14C-labeled proteins were isolated from a water extract of the oil-free seed meal. The specific activities of the two proteins and of some amino acids isolated from them suggested rate differences in protein synthesis, or, alternatively, conversion of one protein into another. The results also showed that synthesis of these proteins occurs over a considerable period in the plant's development.

Induction of microspore embryogenesis in Brassica napus L. is accompanied by specific changes in protein synthesis

Planta ◽  
1994 ◽  
Vol 195 (1) ◽  
pp. 50-56 ◽  
Author(s):  
Jan H. G. Cordewener ◽  
Ronald Busink ◽  
Jan A. Traas ◽  
Jan B. M. Custers ◽  
Hans J. M. Dons ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Brassica Napus ◽  
Microspore Embryogenesis ◽  
Brassica Napus L

Analysis of embryo, cytoplasmic and maternal genetic correlations for seven essential amino acids in rapeseed meal (Brassica napus L.)

2011 ◽  
Vol 90 (1) ◽  
pp. 67-74 ◽  
Author(s):  
GUO LIN CHEN ◽  
JIAN GUO WU ◽  
MURALI-TOTTEKKAAD VARIATH ◽  
ZHONG WEI YANG ◽  
CHUN HAI SHI
Keyword(s):  
Amino Acids ◽  
Brassica Napus ◽  
Genetic Correlations ◽  
Rapeseed Meal ◽  
Essential Amino Acids ◽  
Brassica Napus L

Species and varietal differences in the proteins of rapeseed

1969 ◽  
Vol 47 (5) ◽  
pp. 679-685 ◽  
Author(s):  
A. J. Finlayson ◽  
R. S. Bhatty ◽  
C. M. Christ
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Brassica Napus ◽  
Amino Acid Composition ◽  
Protein Content ◽  
Acid Composition ◽  
Brassica Campestris ◽  
Sulfur Deficiency ◽  
Brassica Napus L ◽  
Sulfur Containing

Two proteins, previously described by the authors as BI (S020, buffer 12 S) and AIVS (S020, w 1.7 S) have been isolated from eight varieties of rapeseed belonging to Brassica campestris L. and Brassica napus L. species. These proteins have similar chromatographic and electrophoretic characteristics but differ in amino acid composition, particularly with regard to the sulfur-containing amino acids. One of the rapeseed samples was obtained from plants grown on sulfur-deficient soil. The sulfur deficiency produced, in the mature seed, a much reduced protein content and appeared to affect the structure of the protein BI.

Effect of time of sowing on oil content, erucic acid and glucosinolate contents in rapeseed (Brassica napus L. cv. Marnoo)

1986 ◽  
Vol 26 (5) ◽  
pp. 607 ◽  
Author(s):  
JP Sang ◽  
CA Bluett ◽  
BR Elliott ◽  
RJW Truscott
Keyword(s):  
Brassica Napus ◽  
Erucic Acid ◽  
Oil Content ◽  
Seed Meal ◽  
Glucosinolate Content ◽  
Brassica Napus L ◽  
Sowing Time ◽  
Meal Quality

The effect of time of sowing on the oil and meal quality of rapeseed (Brassica napus L. cv. Marnoo) was investigated at Werribee, Victoria, during 198 1 and 1982. In both years oil content declined with later sowing but the proportion of erucic acid in the oil was unaffected by sowing time. In contrast, later sowing increased the glucosinolate content of the seed meal, with July-September sowings exceeding the Canola standard. This increase was due primarily to the increased content of 2-hydroxybut-3- enyl glucosinolate (progoitrin).

Effects of applied sulfur on glucosinolate and oil concentrations in the seeds of rape (Brassica napus L.) and turnip rape (Brassica rapa L. var. silvestris (Lam.) Briggs)

1989 ◽  
Vol 40 (3) ◽  
pp. 617 ◽  
Author(s):  
RJ Mailer
Keyword(s):  
Brassica Napus ◽  
Brassica Rapa ◽  
Seed Oil ◽  
New South ◽  
Field Trials ◽  
Seed Meal ◽  
Limiting Factor ◽  
Brassica Napus L ◽  
Turnip Rape ◽  
Glucosinolate Concentration

The effect of sulfur availability on glucosinolate concentration in seed meal of glasshouse grown Brassica nupus cv. Wesbrook and Brassica rapa cv. Runyip was studied. In addition, field trials were evaluated to determine the degree of variability of glucosinolate concentrations in the seed and of sulfur in the plants of rapeseed grown at a number of sites throughout New South Wales.Glucosinolate concentration in seeds grown in the glasshouse increased (P < 0.01) in both cultivars with increasing sulfur application, ranging from an average of 5 8mol at 4 8g g-1 to 55 8mol at 100 8g g-1 sulfur. Bunyip containcd significantly higher concentrations (Pt0-01) than Wesbrook. Increased rates of sulfur application resulted in increased (P < 0.01) seed oil concentrations (from 28.7 to 37.6%), yield (3.1-27.1 g) and 1000-grain weights (2.1-2.9 g). Field trials showed site (P < 0.01) and cultivar (P<0.01) variation in glucosinolate levels. However, sulfur did not appear to be an independently limiting factor in field-grown plants.

scholarly journals The formation of sulphur-containing amino acids in germinating seeds of rape (Brassica napus L.)

1972 ◽  
Vol 126 (4) ◽  
pp. 985-991 ◽  
Author(s):  
T. T. Ngo ◽  
P. D. Shargool
Keyword(s):  
Amino Acids ◽  
Brassica Napus ◽  
Specific Radioactivity ◽  
Total Radioactivity ◽  
Brassica Napus L ◽  
Free Sulphur ◽  
Germinating Seeds

Sodium [35S]sulphide was fed to batches of germinating rapeseed, in some instances with the addition of unlabelled cysteine. Both the total radioactivity and specific radioactivity of the free sulphur-containing amino acids were examined. Cysteine and homocysteine were rapidly labelled; label subsequently appeared in cystathionine and methionine. The results obtained indicated that both the sulphydration and trans-sulphuration pathways were operating. This conclusion was reinforced by the results of experiments in which batches of rapeseed were incubated with l-[14C]homoserine. These showed the formation of labelled homocysteine, cystathione and methionine. It was thought the trans-sulphuration pathway was making the greater contribution to the biosynthesis of methionine in germinating rapeseed.

Rapeseed meal autolysis. Formation of diastereomeric (2R)-1-cyano-2-hydroxy-3,4-epithiobutanes from progoitrin

1967 ◽  
Vol 45 (17) ◽  
pp. 1971-1974 ◽  
Author(s):  
M. E. Daxenbichler ◽  
C. H. VanEtten ◽  
W. H. Tallent ◽  
I. A. Wolff
Keyword(s):  
Brassica Napus ◽  
Rapeseed Meal ◽  
Chiral Center ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Seed Meal ◽  
Optical Rotatory ◽  
Brassica Napus L ◽  
Crambe Abyssinica ◽  
Dispersion Data

When defatted seed from Brassica napus L. var. Regina II (a rapeseed) was autolyzed, its major thioglucoside (progoitrin) underwent degradation analogous to that for epiprogoitrin in Crambe abyssinica seed meal. Depending on the conditions, (R)-1-cyano-2-hydroxy-3-butene and the diastereomeric forms of (2R)-1-cyano-2-hydroxy-3,4-epithiobutane were formed in autolyzed B. napus meal instead of (S)-goitrin. The configuration at the chiral center containing the hydroxyl is assigned on the basis of known data. The configuration at carbon 3 of the episulfides is predicted on the basis of the optical rotatory dispersion data.

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