scholarly journals The formation of sulphur-containing amino acids in germinating seeds of rape (Brassica napus L.)

1972 ◽  
Vol 126 (4) ◽  
pp. 985-991 ◽  
Author(s):  
T. T. Ngo ◽  
P. D. Shargool
Keyword(s):  
Amino Acids ◽  
Brassica Napus ◽  
Specific Radioactivity ◽  
Total Radioactivity ◽  
Brassica Napus L ◽  
Free Sulphur ◽  
Germinating Seeds

Sodium [35S]sulphide was fed to batches of germinating rapeseed, in some instances with the addition of unlabelled cysteine. Both the total radioactivity and specific radioactivity of the free sulphur-containing amino acids were examined. Cysteine and homocysteine were rapidly labelled; label subsequently appeared in cystathionine and methionine. The results obtained indicated that both the sulphydration and trans-sulphuration pathways were operating. This conclusion was reinforced by the results of experiments in which batches of rapeseed were incubated with l-[14C]homoserine. These showed the formation of labelled homocysteine, cystathione and methionine. It was thought the trans-sulphuration pathway was making the greater contribution to the biosynthesis of methionine in germinating rapeseed.

Analysis of embryo, cytoplasmic and maternal genetic correlations for seven essential amino acids in rapeseed meal (Brassica napus L.)

2011 ◽  
Vol 90 (1) ◽  
pp. 67-74 ◽  
Author(s):  
GUO LIN CHEN ◽  
JIAN GUO WU ◽  
MURALI-TOTTEKKAAD VARIATH ◽  
ZHONG WEI YANG ◽  
CHUN HAI SHI
Keyword(s):  
Amino Acids ◽  
Brassica Napus ◽  
Genetic Correlations ◽  
Rapeseed Meal ◽  
Essential Amino Acids ◽  
Brassica Napus L

Species and varietal differences in the proteins of rapeseed

1969 ◽  
Vol 47 (5) ◽  
pp. 679-685 ◽  
Author(s):  
A. J. Finlayson ◽  
R. S. Bhatty ◽  
C. M. Christ
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Brassica Napus ◽  
Amino Acid Composition ◽  
Protein Content ◽  
Acid Composition ◽  
Brassica Campestris ◽  
Sulfur Deficiency ◽  
Brassica Napus L ◽  
Sulfur Containing

Two proteins, previously described by the authors as BI (S020, buffer 12 S) and AIVS (S020, w 1.7 S) have been isolated from eight varieties of rapeseed belonging to Brassica campestris L. and Brassica napus L. species. These proteins have similar chromatographic and electrophoretic characteristics but differ in amino acid composition, particularly with regard to the sulfur-containing amino acids. One of the rapeseed samples was obtained from plants grown on sulfur-deficient soil. The sulfur deficiency produced, in the mature seed, a much reduced protein content and appeared to affect the structure of the protein BI.

INCORPORATION OF 14C INTO TWO PROTEINS FROM RAPESEED (BRASSICA NAPUS L.)

1966 ◽  
Vol 44 (7) ◽  
pp. 997-1004 ◽  
Author(s):  
A. J. Finlayson
Keyword(s):  
Amino Acids ◽  
Protein Synthesis ◽  
Brassica Napus ◽  
Sodium Acetate ◽  
Water Extract ◽  
Seed Meal ◽  
Brassica Napus L ◽  
Specific Activities ◽  
Considerable Period

Four groups of five rapeseed plants (Brassica napus L.) were injected with sodium acetate-2-14C at intervals between flowering and maturity. Two 14C-labeled proteins were isolated from a water extract of the oil-free seed meal. The specific activities of the two proteins and of some amino acids isolated from them suggested rate differences in protein synthesis, or, alternatively, conversion of one protein into another. The results also showed that synthesis of these proteins occurs over a considerable period in the plant's development.

Para-Fluorophenylalanine-induced chromosome number changes in higher plants. II. Tomato and rape

Genome ◽  
1991 ◽  
Vol 34 (6) ◽  
pp. 929-934 ◽  
Author(s):  
P. Banks ◽  
E. J. Britten ◽  
G. H. Gordon
Keyword(s):  
Brassica Napus ◽  
Chromosome Number ◽  
Lycopersicon Esculentum ◽  
Chromosome Numbers ◽  
Higher Plants ◽  
Morphological Changes ◽  
Brassica Napus L ◽  
Tomato Seedlings ◽  
Lycopersicon Esculentum Mill ◽  
Germinating Seeds

Germinating seeds of rape (Brassica napus L.) and of tomato (Lycopersicon esculentum Mill.), and 1-month-old tomato seedlings, were treated with para-fluorophenylalanine to change chromosome numbers. A number of morphological aberrations were found in treated plants of both species, some of which were also observed in progeny of treated tomatoes. Aneuploids and polyploids were confirmed cytologically in treated plants of both species. The investigations confirm the suggestion previously made for maize, that para-fluorophenylalanine is capable of changing chromosome numbers and the resulting morphology of plants of widely different phylogenetic categories.Key words: Para-fluorophenylalanine, chromosome number changes, plants, tomato, rape, aneuploids, polyploids, morphological changes.

scholarly journals Metabotyping: A New Approach to Investigate Rapeseed (Brassica napus L.) Genetic Diversity in the Metabolic Response to Clubroot Infection

2012 ◽  
Vol 25 (11) ◽  
pp. 1478-1491 ◽  
Author(s):  
Geoffrey Wagner ◽  
Sophie Charton ◽  
Christine Lariagon ◽  
Anne Laperche ◽  
Raphaël Lugan ◽  
...  
Keyword(s):  
Amino Acids ◽  
Brassica Napus ◽  
Root System ◽  
Metabolic Response ◽  
Plasmodiophora Brassicae ◽  
Late Response ◽  
Primary Metabolism ◽  
Brassica Napus L ◽  
Clubroot Disease ◽  
Metabolic Sink

Clubroot disease affects all Brassicaceae spp. and is caused by the obligate biotroph pathogen Plasmodiophora brassicae. The development of galls on the root system is associated with the establishment of a new carbon metabolic sink. Here, we aimed to deepen our knowledge of the involvement of primary metabolism in the Brassica napus response to clubroot infection. We studied the dynamics and the diversity of the metabolic responses to the infection. Root system metabotyping was carried out for 18 rapeseed genotypes displaying different degrees of symptom severity, under inoculated and noninoculated conditions at 42 days postinoculation (dpi). Clubroot susceptibility was positively correlated with clubroot-induced accumulation of several amino acids. Although glucose and fructose accumulated in some genotypes with minor symptoms, their levels were negatively correlated to the disease index across the whole set of genotypes. The dynamics of the metabolic response were studied for the susceptible genotype ‘Yudal,’ which allowed an “early” metabolic response (established from 14 to 28 dpi) to be differentiated from a “late” response (from 35 dpi). We discuss the early accumulation of amino acids in the context of the establishment of a nitrogen metabolic sink and the hypothetical biological role of the accumulation of glutathione and S-methylcysteine.

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