STUDIES ON THE IN VITRO ANTITUMOR ACTIVITY OF FATTY ACIDS: IV. THE ESTERS OF ACIDS CLOSELY RELATED TO 10-HYDROXY- 2-DECENOIC ACID FROM ROYAL JELLY AGAINST TRANSPLANTABLE MOUSE LEUKEMIA

1961 ◽  
Vol 39 (11) ◽  
pp. 1765-1770 ◽  
Author(s):  
Gordon F. Townsend ◽  
William H. Brown ◽  
Ethel E. Felauer ◽  
Barbara Hazlett
Keyword(s):  
Fatty Acids ◽  
Royal Jelly ◽  
Dicarboxylic Acids ◽  
Leukemic Cells ◽  
Straight Chain ◽  
Decenoic Acid ◽  
Monocarboxylic Acids ◽  
Mouse Leukemia ◽  
Related Compounds

Previous studies have shown that whole royal jelly, a fraction from royal jelly (10-hydroxy-2-decenoic acid), and certain closely related dicarboxylic acids, some of which are also found in royal jelly, will inhibit the development of transplantable AKR leukemia when the pH is below 5.6.The ester of 10-hydroxy-2-decenoic acid from royal jelly was found to be just as effective against AKR leukemic cells as the acid itself, with the added advantage that it could be used at neutrality.Through the testing of a series of mono- and di-carboxylic acids, as well as other closely related compounds, the activity has been shown to be associated mainly with 9- and 10-carbon straight chain monocarboxylic acids either saturated or unsaturated. Slight variations in the structure either reduce or destroy the activity.

STUDIES ON THE IN VITRO ANTITUMOR ACTIVITY OF FATTY ACIDS: II. SATURATED DICARBOXYLIC ACIDS

1960 ◽  
Vol 38 (6) ◽  
pp. 597-603 ◽  
Author(s):  
Joseph F. Morgan ◽  
Susan Tolnai ◽  
Gordon F. Townsend
Keyword(s):  
Fatty Acids ◽  
Antitumor Activity ◽  
Royal Jelly ◽  
Dicarboxylic Acids ◽  
Ehrlich Carcinoma ◽  
Decenoic Acid ◽  
Fatty Acid Chain ◽  
Transplantable Leukemia ◽  
Akr Mice

Previous studies, which showed that 10-hydroxy-2-decenoic acid from royal jelly possessed in vitro antitumor activity, have been extended to saturated dicarboxylic fatty acids. Seven of eight compounds tested in a series of chain length from C3 to C10 completely prevented the development of the ascites forms of the 6C3HED lymphosarcoma, the Ehrlich carcinoma, and the TA3 mammary carcinoma, as well as the transplantable leukemia of AKR mice. This in vitro antitumor activity could be demonstrated only at pH values below 5.0 and required admixture of the tumor cells and test compounds prior to inoculation of the mice. In general, the antitumor activity of the saturated dicarboxylic acids was found to increase progressively with increasing length of the fatty acid chain.

STUDIES ON THE IN VITRO ANTITUMOR ACTIVITY OF FATTY ACIDS: II. SATURATED DICARBOXYLIC ACIDS

1960 ◽  
Vol 38 (1) ◽  
pp. 597-603 ◽  
Author(s):  
Joseph F. Morgan ◽  
Susan Tolnai ◽  
Gordon F. Townsend
Keyword(s):  
Fatty Acids ◽  
Antitumor Activity ◽  
Royal Jelly ◽  
Dicarboxylic Acids ◽  
Ehrlich Carcinoma ◽  
Decenoic Acid ◽  
Fatty Acid Chain ◽  
Transplantable Leukemia ◽  
Akr Mice

Previous studies, which showed that 10-hydroxy-2-decenoic acid from royal jelly possessed in vitro antitumor activity, have been extended to saturated dicarboxylic fatty acids. Seven of eight compounds tested in a series of chain length from C3 to C10 completely prevented the development of the ascites forms of the 6C3HED lymphosarcoma, the Ehrlich carcinoma, and the TA3 mammary carcinoma, as well as the transplantable leukemia of AKR mice. This in vitro antitumor activity could be demonstrated only at pH values below 5.0 and required admixture of the tumor cells and test compounds prior to inoculation of the mice. In general, the antitumor activity of the saturated dicarboxylic acids was found to increase progressively with increasing length of the fatty acid chain.

STUDIES ON THE IN VITRO ANTITUMOR ACTIVITY OF FATTY ACIDS: III. SATURATED MONOCARBOXYLIC ACIDS

1961 ◽  
Vol 39 (4) ◽  
pp. 713-719 ◽  
Author(s):  
Susan Tolnai ◽  
Joseph F. Morgan
Keyword(s):  
Fatty Acids ◽  
Antitumor Activity ◽  
Royal Jelly ◽  
Decanoic Acid ◽  
Carbon Chain ◽  
Ehrlich Carcinoma ◽  
Carbon Chain Length ◽  
Monocarboxylic Acids ◽  
Ph Conditions

Previous studies on the in vitro antitumor activity of 10-hydroxy-2-decenoic acid from royal jelly and of saturated dibasic fatty acids have been extended to a series of saturated monocarboxylic acids ranging from C3to C18. A widespread ability to inhibit the development of the ascites forms of the 6C3HED lymphosarcoma, Ehrlich carcinoma, and TA3mammary carcinoma has been found under acid pH conditions. In general, the antitumor activity of the saturated monocarboxylic acids was most pronounced in compounds with a carbon chain length of C8to C14. One member of this series, capric (decanoic) acid, was found to possess marked antitumor activity at physiological pH.

TetR-family transcriptional repressor Thermus thermophilus FadR controls fatty acid degradation

Microbiology ◽  
2011 ◽  
Vol 157 (6) ◽  
pp. 1589-1601 ◽  
Author(s):  
Yoshihiro Agari ◽  
Kazuko Agari ◽  
Keiko Sakamoto ◽  
Seiki Kuramitsu ◽  
Akeo Shinkai
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Target Genes ◽  
Thermus Thermophilus ◽  
Acyl Chain ◽  
Metabolic Energy ◽  
Straight Chain ◽  
Fatty Acid Degradation ◽  
Acid Degradation

In the extremely thermophilic bacterium Thermus thermophilus HB8, one of the four TetR-family transcriptional regulators, which we named T. thermophilus FadR, negatively regulated the expression of several genes, including those involved in fatty acid degradation, both in vivo and in vitro. T. thermophilus FadR repressed the expression of the target genes by binding pseudopalindromic sequences covering the predicted −10 hexamers of their promoters, and medium-to-long straight-chain (C10–18) fatty acyl-CoA molecules were effective for transcriptional derepression. An X-ray crystal structure analysis revealed that T. thermophilus FadR bound one lauroyl (C12)-CoA molecule per FadR monomer, with its acyl chain moiety in the centre of the FadR molecule, enclosed within a tunnel-like substrate-binding pocket surrounded by hydrophobic residues, and the CoA moiety interacting with basic residues on the protein surface. The growth of T. thermophilus HB8, with palmitic acid as the sole carbon source, increased the expression of FadR-regulated genes. These results indicate that in T. thermophilus HB8, medium-to-long straight-chain fatty acids can be used for metabolic energy under the control of FadR, although the major fatty acids found in this strain are iso- and anteiso-branched-chain (C15 and 17) fatty acids.

scholarly journals Royal Jelly Aliphatic Acids Contribute to Antimicrobial Activity of Honey

2018 ◽  
Vol 62 (1) ◽  
pp. 111-123 ◽  
Author(s):  
Walerij Isidorow ◽  
Stanisław Witkowski ◽  
Piotr Iwaniuk ◽  
Monika Zambrzycka ◽  
Izabela Swiecicka
Keyword(s):  
Fatty Acids ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Royal Jelly ◽  
Dicarboxylic Acids ◽  
Antibacterial Action ◽  
Larval Food ◽  
Nutrient Media ◽  
Minimal Inhibitory Concentrations ◽  
Aliphatic Acids

Abstract Honey is valued for its therapeutic qualities which are attributed among others to its antibacterial multifactorial properties. However, all the factors that influence these properties have not been identified. The present study is focused on the antibacterial action of fatty acids originating from royal jelly, the larval food of honeybees. Aliphatic C8-C12 acids characteristic of this bee product had previously been identified in more than fifty different samples of honey originating from seven countries and in eleven samples of Polish herbhoney. Experiments were performed to ascertain the influence of acidity on the antimicrobial activity of the acids. In acidic nutrient media all tested aliphatic hydroxyacids and unsaturated dicarboxylic acids demonstrated antibacterial action against different microbes with minimal inhibitory concentrations between 0.048 and 3.125 mM. Our results confirm that part of the antibacterial activity of honey contributes to these compounds of bee origin.

The Effect of Fatty Acids upon Tumor Cell Respiration and Transplantability

1963 ◽  
Vol 9 (5) ◽  
pp. 530-543 ◽  
Author(s):  
Bernard J Katchman ◽  
Robert E Zipf ◽  
James P F Murphy
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Tumor Cells ◽  
Chemical Structure ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Leukemic Cells ◽  
Endogenous Respiration ◽  
Low Concentrations

Abstract The kinetic effect of palmitate, stearate, oleate, linoleate, and linolenate upon in vitro endogenous respiration of rat chloromyeloid leukemic cells has been investigated. Inhibition of respiration has been correlated with the ability of fatty acids to cause decreased cell viability and cell count; in the bioassay of fatty acid-treated tumor inocula, the increase in animal life span is correlated to the degree of dilution of the inocula due to cell lysis. The degree of lysis is dependent upon the chemical structure of the fatty acid, concentration, and duration of contact; unsaturated fatty acids are more effective than saturated fatty acids. Tumor cells, when incubated at low concentrations of fatty acids, show stimulation of O2 uptake; however, in the bioassay these fatty acid-treated inocula showed no loss in tumor activity. The nature of the physiochemical interaction between fatty acids and tumor cells is discussed.

Fatty acids isolated from royal jelly modulate dendritic cell-mediated immune response in vitro

2007 ◽  
Vol 7 (9) ◽  
pp. 1211-1220 ◽  
Author(s):  
Dragana Vucevic ◽  
Eleni Melliou ◽  
Sasa Vasilijic ◽  
Sonja Gasic ◽  
Petar Ivanovski ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Immune Response ◽  
Dendritic Cell ◽  
Royal Jelly ◽  
Cell Mediated Immune Response ◽  
Immune Response In Vitro

scholarly journals In VitroAnti-Inflammatory Effects of Three Fatty Acids from Royal Jelly

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Yi-Fan Chen ◽  
Kai Wang ◽  
Yan-Zheng Zhang ◽  
Yu-Fei Zheng ◽  
Fu-Liang Hu
Keyword(s):  
Fatty Acids ◽  
Royal Jelly ◽  
Inflammatory Diseases ◽  
Sebacic Acid ◽  
Interleukin 10 ◽  
Raw 264.7 Macrophages ◽  
Anti Inflammatory ◽  
Underlying Mechanisms ◽  
Dose Dependent

Trans-10-hydroxy-2-decenoic acid (10-H2DA), 10-hydroxydecanoic acid (10-HDAA), and sebacic acid (SEA) are the three major fatty acids in royal jelly (RJ). Previous studies have revealed several pharmacological activities of 10-H2DA and 10-HDAA, although the anti-inflammatory effects and underlying mechanisms by which SEA acts are poorly understood. In the present study, we evaluated and compared thein vitroanti-inflammatory effects of these RJ fatty acids in lipopolysaccharide-stimulated RAW 264.7 macrophages. The results showed that 10-H2DA, 10-HDAA, and SEA had potent, dose-dependent inhibitory effects on the release of the major inflammatory-mediators, nitric oxide, and interleukin-10, and only SEA decreased TNF-αproduction. Several key inflammatory genes have also been modulated by these RJ fatty acids, with 10-H2DA showing distinct modulating effects as compared to the other two FAs. Furthermore, we found that these three FAs regulated several proteins involved in MAPK and NF-κB signaling pathways. Taken together, these findings provide additional references for using RJ against inflammatory diseases.

scholarly journals Molecular composition of dicarboxylic acids, ketocarboxylic acids, α-dicarbonyls and fatty acids in atmospheric aerosols from Tanzania, East Africa during wet and dry seasons

2013 ◽  
Vol 13 (4) ◽  
pp. 2235-2251 ◽  
Author(s):  
S. L. Mkoma ◽  
K. Kawamura
Keyword(s):  
Fatty Acids ◽  
Dicarboxylic Acids ◽  
Dry Season ◽  
Water Soluble ◽  
Molecular Composition ◽  
Wet Season ◽  
Dry Seasons ◽  
Pm2.5 And Pm10 ◽  
Related Compounds ◽  
Wet And Dry Seasons

Abstract. Atmospheric aerosol samples of PM2.5 and PM10 were collected during the wet and dry seasons in 2011 from a rural site in Tanzania and analysed for water-soluble dicarboxylic acids, ketocarboxylic acids, α-dicarbonyls, and fatty acids using a gas chromatography/flame ionization detector (GC/FID) and GC/mass spectrometry. Here we report the molecular composition and sources of diacids and related compounds for wet and dry seasons. Oxalic acid (C2) was found as the most abundant diacid species followed by succinic and/or malonic acids whereas glyoxylic acid and glyoxal were the dominant ketoacid and α-dicarbonyl, respectively in both seasons in PM2.5 and PM10. Mean concentration of C2 in PM2.5 (121 ± 47 ng m−3) was lower in wet season than dry season (258 ± 69 ng m−3). Similarly, PM10 samples showed lower concentration of C2 (169 ± 42 ng m−3) in wet season than dry season (292 ± 165 ng m−3). Relative abundances of C2 in total diacids were 65% and 67% in PM2.5 and 65% and 64% in PM10 in the wet and dry seasons, respectively. Total concentrations of diacids (289–362 ng m−3), ketoacids (37.8–53.7 ng m−3), and α-dicarbonyls (5.7–7.8 ng m−3) in Tanzania are higher than those reported at a rural background site in Nylsvley (South Africa) but comparable or lower than those reported from sites in Asia and Europe. Diacids and ketoacids were found to be present mainly in PM2.5 in both seasons (total α-dicarbonyls in the dry season), suggesting a production of organic acids from pyrogenic sources and photochemical oxidations. Averaged contributions of total diacids to aerosol total carbon were 1.4% in PM2.5 and 2.1% in PM10 during wet season and 3.3% in PM2.5 and 3.9% in PM10 during dry season whereas those to water-soluble organic carbon were 2.2% and 4.7% in PM2.5 during wet season and 3.1% and 5.8% in PM10 during dry season. The higher ratios in dry season suggest an enhanced photochemical oxidation of organic precursors probably via heterogeneous reactions on aerosols under strong solar radiation. Strong positive correlations were found among diacids and related compounds as well as good relations to source tracers in both seasons, suggesting a mixed source from natural biogenic emissions, biomass burning, biofuel combustion, and photochemical production.

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