Low molecular weight complexed poly(3-hydroxybutyrate): a dynamic and versatile molecule in vivo

1995 ◽  
Vol 41 (13) ◽  
pp. 50-54 ◽  
Author(s):  
Rosetta N. Reusch
Keyword(s):  
Molecular Weight ◽  
Chemical Properties ◽  
Low Molecular Weight ◽  
Physical And Chemical Properties ◽  
Biological Cells ◽  
Ion Conducting ◽  
Physical And Chemical ◽  
Electron Donating Groups ◽  
And Chemical Properties

It is increasingly clear that poly(3-hydroxybutyrate) (PHB) is not just an inert storage polymer, confined to certain bacteria, but a ubiquitous, interactive, solvating biopolymer involved in important physiological functions. Low molecular weight PHB, complexed to other macromolecules (c-PHB), is widely distributed in biological cells, being found in representative organisms of nearly all phyla. Complexation modifies the physical and chemical properties of c-PHB, allowing it to pervade aqueous as well as hydrophobic regions of the cell, and as a result c-PHB can be found in cytoplasm and intracellular fluids as well as in membranes and lipoproteins. The lipidic homopolymer associates with other macromolecules primarily via its ester carbonyl oxygens, which can act as hydrogen-bond acceptors or as ligands for coordinate bonds to cations. The spacing of the electron-donating groups along the flexible backbone allows for multiple bonding interactions, and forms the basis for the ability of c-PHB to bind to proteins, or to form ion-conducting complexes with salts. The singular ability of c-PHB to dissolve salts and facilitate their transfer across hydrophobic barriers defines a potential physiological niche for c-PHB in cell metabolism.Key words: polyhydroxybutyrate, polyphosphate, polymer electrolyte, ion transport.

Isolation and identification of the high molecular weight saturated fatty acids of butterfat

1959 ◽  
Vol 26 (2) ◽  
pp. 190-195 ◽  
Author(s):  
R. P. Hansen ◽  
F. B. Shorland ◽  
N. June Cooke
Keyword(s):  
Molecular Weight ◽  
Saturated Fatty Acids ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Octadecanoic Acid ◽  
Isolation And Identification ◽  
Docosanoic Acid ◽  
Physical And Chemical ◽  
Tetracosanoic Acid ◽  
And Chemical Properties

Butterfat has been shown to contain the normal odd-numbered saturated acids n-nonadecanoic acid (C19), n-heneicosanoic acid (C21), and n-tricosanoic acids (C23).The presence of the normal even-numbered acids n-octadecanoic acid (C18), n-docosanoic acid (C22), n-tetracosanoic acid (C24) and n-hexacosanoic acid (C26) is conclusively established.n-Eicosanoic acid (C20) formerly assumed to be present in butterfat has been isolated and identified by its physical and chemical properties.

scholarly journals Physico-Chemical Properties of Purified Carboxylesterase from the Seeds of Tamarindus indica

2021 ◽  
pp. 42-58
Author(s):  
S. Kantharaju ◽  
M. Mylarappa
Keyword(s):  
Molecular Weight ◽  
Gel Filtration ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Tamarindus Indica ◽  
Physico Chemical ◽  
Sds Page ◽  
Physical And Chemical ◽  
Naphthyl Acetate ◽  
And Chemical Properties

The present work is focus on physical and chemical properties of purified Carboxylesterase using the Seeds of Tamarindus Indica.The esterases are extracted from the germinating tamarind seeds using 50 mM phosphate buffer, pH 7 and purified. The Km with α-naphthyl acetate, β-naphthyl acetate and α-naphthyl butyrate as the substrates is 28.6 μM, 22.2 μM and 26.7 μM respectively. The Vmax for the same substrates is 7.1 x 10-3 µmole/min, 7.41 x 10-3 µmole/min and 8.00 x 10-3 µmole/min respectively. The enzymes optimally active at pH 7.0 and are stable between pH 5.0 to 8.0. The optimum temperature of esterase activity is 40˚C. The molecular weight of 27.5 kD as determined by SDS-PAGE, both in the presence and absence of β-mercaptothanol and is in close agreement with the molecular weight determined by gel-filtration on Sephadex G-100 (26.9 kD).

The Kinetics and Occurrence of Optimum Vulcanization

1956 ◽  
Vol 29 (2) ◽  
pp. 555-567
Author(s):  
B. A. Dogadkin
Keyword(s):  
Molecular Weight ◽  
Tensile Strength ◽  
Structure Formation ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Smooth Curves ◽  
Intermolecular Reaction ◽  
Physical And Chemical ◽  
Kinetics Of ◽  
And Chemical Properties

Abstract The fundamental reaction of vulcanization is the combination of a vulcanizing agent with rubber. The kinetics of this reaction is expressed by smooth curves. Simultaneously with the combining of the vulcanizing agent, in fact as a result of it, changes take place in a number of physical and chemical properties of rubber—solubility, modulus, tensile strength, and other indexes. Unlike the kinetics of combination of the vulcanizing agent, the changes in these properties are most often represented by curves having a maximum or minimum which characterizes the phenomenon of optimum vulcanization. The extreme form which curves of changes of physical and chemical properties of rubber assume during vulcanization can be explained, in our opinion, by the fact that, during vulcanization, there is a competition between opposing reactions, of which one set are reactions of structure formation (i.e., increase of the molecular weight and the intensity of intermolecular reaction), and the others are destruction reactions. Thus, during vulcanization under factory conditions, at least two reactions take place: (1) the reaction between rubber and sulfur, and (2) the reaction between rubber and molecular oxygen introduced into the vulcanization mix by milling with the ingredients. The amount of oxvgen present here in moles approaches the molar concentration of sulfur.

Efficacy, safety, and physicochemical properties of a flowable hemostatic agent made from absorbable gelatin sponge via vacuum pressure steam sterilization

2020 ◽  
pp. 088532822095089
Author(s):  
Yuanxing Zhou ◽  
Xiaochi Ma ◽  
Zhonghai Li ◽  
Bo Wang
Keyword(s):  
Chemical Properties ◽  
Erythrocyte Aggregation ◽  
Hemostatic Agent ◽  
Gelatin Sponge ◽  
Toxicity Tests ◽  
Physical And Chemical Properties ◽  
Physical And Chemical ◽  
And Chemical Properties

An effective and viable hemostatic agent is important for stopping bleeding during surgery. However, it is difficult to achieve hemostasis at uneven or deep bleeding sites using a gelatin sponge. A flowable hemostatic agent has therefore been developed by processing and improving gelatin sponge, to address bleeding under these conditions. In this study, we evaluated the efficacy, safety, and physical and chemical properties of this flowable hemostatic agent in various experiments. We examined its efficacy for stopping bleeding in a rabbit model of liver abrasion in vivo, and compared its efficacy in dynamic coagulation and erythrocyte aggregation tests with gelatin sponge in vitro. We also investigated its safety in rat histocompatibility and acute systemic toxicity tests in mice in vivo, and in hemolysis tests in vitro, to determine if the flowable hemostatic agent induced any pathological reactions or adverse events. In terms of its physical and chemical properties, we analyzed the morphology and chemical bonds of the flowable hemostatic agent by optical and electron microscopy and infrared spectroscopy, and its absorbency and density. The flowable hemostatic agent resulted in a shorter mean bleeding time, less bleeding, greater likelihood of successful hemostasis, and reduced clotting time compared with gelatin sponge. The flowable agent produced some changes in physical morphology, but no pathological changes or undesirable outcomes were detected. This flowable topical hemostatic agent thus provides a safe and more effective hemostatic method than gelatin sponge, and more promising results for intraoperative hemostasis, especially on uneven or deep bleeding surfaces.

scholarly journals Zirconia Based Dental Biomaterials: Structure, Mechanical Properties, Biocompatibility, Surface Modification, and Applications as Implant

2021 ◽  
Vol 2 ◽  
Author(s):  
Hua Lin ◽  
Cuilan Yin ◽  
Anchun Mo
Keyword(s):  
Mechanical Properties ◽  
Adverse Reactions ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Zirconia Ceramics ◽  
Innovative Strategies ◽  
Physical And Chemical ◽  
Orthopedic Applications ◽  
And Chemical Properties

Zirconia, with its excellent mechanical properties, chemical stability, biocompatibility, and negligible thermal conductivity, is ideal for dental and orthopedic applications. In addition, the biocompatibility of zirconia has been studied in vivo, and no adverse reactions were observed when zirconia samples were inserted into bone. However, their use is controversial among dentists and researchers, especially when compared with mature implants made of titanium alloy. The advantages and limitations of zirconia as biomaterials, such as implant materials, need to be carefully studied, and the design, manufacture, and clinical operation guidelines are urgently required. In this review, the special components, microstructure, mechanical strength, biocompatibility, and the application of zirconia ceramics in biomaterials are detailly introduced. The review highlights discussions on how to implement innovative strategies to design the physical and chemical properties of zirconia so that the treated zirconia can provide better osteointegration after implantation.

scholarly journals Comparative Study of the Mucoadhesive Properties of Polymers for Pharmaceutical Use

2020 ◽  
Vol 8 (A) ◽  
pp. 639-645
Author(s):  
Elena Bakhrushina ◽  
Maria Anurova ◽  
Natalia Demina ◽  
Alena Kashperko ◽  
Olga Rastopchina ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Xanthan Gum ◽  
Average Molecular Weight ◽  
Negative Relationship ◽  
Chemical Properties ◽  
Drug Formulation ◽  
Movement Speed ◽  
Physical And Chemical Properties ◽  
Physical And Chemical ◽  
And Chemical Properties

BACKGROUND: In recent years, mucoadhesive dosage forms due to their advantages have attracted the interest of researchers and developers. Polymeric excipients are included into the drug composition to give adhesion to the mucous membrane. AIM: The aim of this research was to select a specific brand of pharmaceutical quality polymer that is promising for inclusion in the drug formulation. METHODS: The article presents the results of studying the mucoadhesive properties of polymers on two models using mucin: By measuring the amount of adhesion and by the evaluation the sample movement speed. RESULTS: According to the combination of two indicators, the highest mucoadhesive properties were shown by the brands of hydroxypropylmethylcellulose and xanthan gum. In addition, it was noted that hydroxyethylcellulose (HEC), sodium alginate, and hydroxypropylmethylcellulose (HPMC) also have good mucoadhesive properties. Polyethylene glycols proved to have the weakest mucoadhesive properties. The negative relationship between the average molecular weight and the sample movement speed of the HEC and HPMC was established. Obtained data showed no direct influence of the polymer average molecular weight on the amount of adhesion. It was also noted that there is no strong correlation between the amount of adhesion and the sample movement speed of the experimental samples. CONCLUSION: Based on the results of the study, it was suggested that the complex influences of the physical and chemical properties of the polymer on its mucoadhesive properties.

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