Study of the antigenic relationships between strains of Bacteroides. intermedius, B. melaninogenicus, B. corporis, and B. denticola revealed by immunoblotting with rabbit antisera

1990 ◽  
Vol 36 (9) ◽  
pp. 637-648 ◽  
Author(s):  
G. H. Bowden ◽  
N. Nolette
Keyword(s):  
Key Words ◽  
Porphyromonas Gingivalis ◽  
Western Blotting ◽  
Homology Groups ◽  
Homologous Strain ◽  
Specific Antigens ◽  
Common Antigens ◽  
Species Specific ◽  
Humoral Responses ◽  
Antigenic Relationships

Antigen profiles of saccharolytic oral black-pigmented Bacteroides have been developed by Western blotting. Visual comparisons indicated extensive cross-reactions between B. intermedius, B. melaninogenicus, B. denticola, and B. corporis. Porphyromonas gingivalis, P. asaccharolyticus, and B. buccae showed less cross-reaction. Quantitation of antigenic similarity was made from densitometric scans. Calculation of the Jackard coefficient gave results of 33–72% similarity among the saccharolytic pigmented species, with the two homology groups of B. intermedius separated at 53%. Species were separated below 70%. Subtraction of the profile of a cross-reacting strain from that of the homologous strain also allowed quantitation of similarities. These similarities were lower; the range between species was 4–62%, although the two homology groups of B. intermedius still separated at 50 and 58%. Species were separated below 63%. Sera absorbed with a cross-reacting strain gave reduced reactions with the homologous strain and cross-reacting strains, indicating several common antigens among the four species. The species-specific antigens demonstrated by sera absorbed with cells of cross-reacting species were relatively few (3–6) compared with cross-reacting antigens detected by non-absorbed sera (18–28). The method appears useful to quantitate antigenic similarities among Bacteroides species and strains and allows analysis and quantitation of individual humoral responses in animals to these bacteria. Key words: oral Bacteroides, Porphyromonas, antigenic similarities, immunoblotting.

The participation of common and species-"specific" antigens of mycobacteria in the tuberculin skin reaction

1975 ◽  
Vol 21 (6) ◽  
pp. 774-783 ◽  
Author(s):  
Raymond Turcotte
Keyword(s):  
Skin Reaction ◽  
Guinea Pigs ◽  
Cutaneous Reaction ◽  
Tuberculin Reaction ◽  
Cross Absorption ◽  
Specific Antigens ◽  
The Common ◽  
Common Antigens ◽  
Species Specific

Protoplasmic extracts isolated from four different species of mycobacteria contained common and species-specific antigens. Both the common and the specific antigens were involved in the elicitation of the tuberculin reaction in sensitized guinea pigs. The elimination of the common antigens from the extracts by means of cross absorption with heterologous mycobacterial antibodies led to preparations which, at the doses used in this study, elicited a cutaneous reaction in animals sensitized with the corresponding strains only. Moreover, the tuberculin activity of the common antigens was about the same in animals sensitized either with homologous or heterologous strains.

scholarly journals Trehalose-containing lipooligosaccharides. A new class of species-specific antigens from Mycobacterium.

1983 ◽  
Vol 258 (17) ◽  
pp. 10481-10487 ◽  
Author(s):  
S W Hunter ◽  
R C Murphy ◽  
K Clay ◽  
M B Goren ◽  
P J Brennan
Keyword(s):  
New Class ◽  
Specific Antigens ◽  
Species Specific

scholarly journals Antigenic diversity and seroprevalences of Torque teno viruses in children and adults by ORF2-based immunoassays

2013 ◽  
Vol 94 (2) ◽  
pp. 409-417 ◽  
Author(s):  
Tingting Chen ◽  
Elina Väisänen ◽  
Petri S. Mattila ◽  
Klaus Hedman ◽  
Maria Söderlund-Venermo
Keyword(s):  
Serum Samples ◽  
Phylogenetic Groups ◽  
Single Strain ◽  
New Approach ◽  
Antiviral Antibody ◽  
Specific Igg ◽  
Species Specific ◽  
Multiple Strains ◽  
Antigenic Relationships

Torque teno viruses (TTVs) circulate widely among humans, causing persistent viraemia in healthy individuals. Numerous TTV isolates with high genetic variability have been identified and segregated into 29 species of five major phylogenetic groups. To date, the diversity of TTV sequences, challenges in protein expression and the subsequent lack of serological assays have hampered TTV seroprevalence studies. Moreover, the antigenic relationships of different TTVs and their specific seroprevalences in humans remain unknown. For five TTV strains – belonging to different species of four genogroups – we developed, using recombinant glutathione S-transferase (GST)-fused TTV ORF2 proteins, glutathione–GST capture enzyme immunoassays (EIAs) detecting antibodies towards conformational epitopes. We then analysed serum samples from 178 healthy adults and 108 children; IgG reactivities were observed either towards a single strain or towards multiple strains, which pointed to antigenic distinction of TTV species. The overall seroprevalence for the five TTVs peaked at 43 % (18 of 42) in children 2–4 years of age, subsequently declined, and again reached 42 % (74 of 178) among adults. TTV6 species-specific IgG predominated in children, whereas that for TTV13 predominated in adults. During a 3 year follow-up of the same children, both species-specific seroconversions and seroreversions occurred. This is the first EIA-based study of different TTVs, providing a new approach for seroepidemiology and diagnosis of TTV infections. Our data suggest that different TTVs in humans may differ in antiviral antibody profiles, infection patterns and epidemiology.

scholarly journals Importance of serological cross-reactivity amongToxoplasma gondii, Hammondiaspp.,Neosporaspp.,Sarcocystisspp. andBesnoitia besnoiti

Parasitology ◽  
2017 ◽  
Vol 144 (7) ◽  
pp. 851-868 ◽  
Author(s):  
LUÍS F. P. GONDIM ◽  
JOSÉ R. MINEO ◽  
GEREON SCHARES
Keyword(s):  
Toxoplasma Gondii ◽  
Neospora Caninum ◽  
Cross Reactivity ◽  
Besnoitia Besnoiti ◽  
Cross Reactions ◽  
Epidemiological Surveys ◽  
Specific Proteins ◽  
Specific Antigens ◽  
Species Specific

SUMMARYToxoplasma gondii, Neosporaspp.,Sarcocystisspp.,Hammondiaspp. andBesnoitia besnoitiare genetically related cyst-forming coccidia. Serology is frequently used for the identification ofT. gondii, Neosporaspp. andB. besnoiti-exposed individuals. Serologic cross-reactions occur in different tests among animals infected withT. gondiiandH. hammondi,as well as among animals infected byT. gondiiandN. caninum. Infections caused byN. caninumandN. hughesiare almost indistinguishable by serology.Neospora caninum, B. besnoitiandSarcocystisspp. infections in cattle show some degree of serologic cross-reactivity. Antibody cross-reactivity betweenNeosporaspp. andH. heydorni-infected animals is suspected, but not proven to occur. We review serologic cross-reactivity among animals and/or humans infected withT. gondii, Neosporaspp.,Sarcocystisspp.,Hammondiaspp. andB. besnoiti. Emphasis is laid upon antigens and serological methods forN. caninumdiagnosis which were tested for cross-reactivity with related protozoa. Species-specific antigens, as well as stage-specific proteins have been identified in some of these parasites and have promising use for diagnosis and epidemiological surveys.

scholarly journals Mutualistic Biofilm Communities Develop with Porphyromonas gingivalis and Initial, Early, and Late Colonizers of Enamel

2009 ◽  
Vol 191 (22) ◽  
pp. 6804-6811 ◽  
Author(s):  
Saravanan Periasamy ◽  
Paul E. Kolenbrander
Keyword(s):  
Porphyromonas Gingivalis ◽  
Dental Plaque ◽  
Fusobacterium Nucleatum ◽  
Single Species ◽  
Biofilm Growth ◽  
Flow Cells ◽  
Tooth Cleaning ◽  
Plaque Development ◽  
Species Specific

ABSTRACT Porphyromonas gingivalis is present in dental plaque as early as 4 h after tooth cleaning, but it is also associated with periodontal disease, a late-developing event in the microbial successions that characterize daily plaque development. We report here that P. gingivalis ATCC 33277 is remarkable in its ability to interact with a variety of initial, early, middle, and late colonizers growing solely on saliva. Integration of P. gingivalis into multispecies communities was investigated by using two in vitro biofilm models. In flow cells, bacterial growth was quantified using fluorescently conjugated antibodies against each species, and static biofilm growth on saliva-submerged polystyrene pegs was analyzed by quantitative real-time PCR using species-specific primers. P. gingivalis could not grow as a single species or together with initial colonizer Streptococcus oralis but showed mutualistic growth when paired with two other initial colonizers, Streptococcus gordonii and Actinomyces oris, as well as with Veillonella sp. (early colonizer), Fusobacterium nucleatum (middle colonizer), and Aggregatibacter actinomycetemcomitans (late colonizer). In three-species flow cells, P. gingivalis grew with Veillonella sp. and A. actinomycetemcomitans but not with S. oralis and A. actinomycetemcomitans. Also, it grew with Veillonella sp. and F. nucleatum but not with S. oralis and F. nucleatum, indicating that P. gingivalis and S. oralis are not compatible. However, P. gingivalis grew in combination with S. gordonii and S. oralis, demonstrating its ability to overcome the incompatibility when cultured with a second initially colonizing species. Collectively, these data help explain the observed presence of P. gingivalis at all stages of dental plaque development.

Gene cloning of Porphyromonas gingivalis specific antigens recognized by serum of adult periodontitis patient

1992 ◽  
Vol 24 (6) ◽  
pp. 945-950 ◽  
Author(s):  
M. Hayakawa ◽  
Y. Abiko ◽  
T. Ito ◽  
H. Sasahara ◽  
H. Yamano ◽  
...  
Keyword(s):  
Gene Cloning ◽  
Porphyromonas Gingivalis ◽  
Periodontitis Patient ◽  
Specific Antigens
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