scholarly journals Antioxidant system blood enzymes activity in patients with echinococcosis

2014 ◽  
Vol 95 (4) ◽  
pp. 531-533 ◽  
Author(s):  
N A Maslennikova ◽  
E Yu Sergeeva ◽  
E P Tikhonova ◽  
A Yu Burakov ◽  
N M Titova ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Catalase Activity ◽  
Treatment Options ◽  
The Body ◽  
Superoxide Dismutase Activity ◽  
Control Group ◽  
Lipid Peroxidation Product ◽  
Gradual Development ◽  
Peroxidation Product ◽  
New Treatment

Aim. To study the activity of antioxidant enzymes and concentration of malondialdehyde - lipid peroxidation product - in patients with echinococcosis. Methods. 22 patients aged 32 to 74 years [females - 9 (40%), males - 13 (60%), mean age 37.6 years] with established diagnosis of liver echinococcosis confirmed by ELISA, were examined. Mean disease duration was 10 years. 12 patients, in whom the diagnosis of liver echinococcosis was excluded, were included in the control group. Superoxide dismutase activity was determined based on adrenaline auto-oxidation inhibition, as well as catalase activity and malondialdehyde level were determined. Results. Statistically significant decrease in the malondialdehyde concentration from 5.9 [5.6÷6.6] to 3.7 [3.3÷4.1] mmol/g of hemoglobin (p 0.001), superoxide dismutase activity - from 764 [744÷796] to 666 [627÷695] i.u./min.×g of hemoglobin (p 0.001) was revealed in patients with echinococcosis. Catalase activity did not change significantly (maintained at the level of control group), which may indicate a temporary activation of the compensatory abilities of the body. Conclusion. Revealed data suggest that gradual development of oxidative stress and decrease in body adaptive abilities play a significant role in the pathogenesis of liver echinococcosis which should be considered while working out the new treatment options for the disease.

scholarly journals In VivoAntioxidant Activity of Deacetylasperulosidic Acid in Noni

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
De-Lu Ma ◽  
Mai Chen ◽  
Chen X. Su ◽  
Brett J. West
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Antioxidant Properties ◽  
Superoxide Dismutase Activity ◽  
Morinda Citrifolia ◽  
Control Group ◽  
Dose Dependent

Deacetylasperulosidic acid (DAA) is a major phytochemical constituent ofMorinda citrifolia(noni) fruit. Noni juice has demonstrated antioxidant activityin vivoand in human trials. To evaluate the role of DAA in this antioxidant activity, Wistar rats were fed 0 (control group), 15, 30, or 60 mg/kg body weight per day for 7 days. Afterwards, serum malondialdehyde concentration and superoxide dismutase and glutathione peroxidase activities were measured and compared among groups. A dose-dependent reduction in malondialdehyde was evident as well as a dose-dependent increase in superoxide dismutase activity. DAA ingestion did not influence serum glutathione peroxidase activity. These results suggest that DAA contributes to the antioxidant activity of noni juice by increasing superoxide dismutase activity. The fact that malondialdehyde concentrations declined with increased DAA dose, despite the lack of glutathione peroxidase-inducing activity, suggests that DAA may also increase catalase activity. It has been previously reported that noni juice increases catalase activityin vivobut additional research is required to confirm the effect of DAA on catalase. Even so, the current findings do explain a possible mechanism of action for the antioxidant properties of noni juice that have been observed in human clinical trials.

scholarly journals Activity of antioxidant enzymes and processes of free radical oxidation in experimental hypothyroidism and correction of thyroid shifts with iodized polysaccharide complexes

2012 ◽  
Vol 93 (1) ◽  
pp. 116-119
Author(s):  
F Kh Kamilov ◽  
A N Mamtsev ◽  
V N Kozlov ◽  
G M Abdullina ◽  
O V Lobyreva
Keyword(s):  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Glutathione Peroxidase ◽  
Catalase Activity ◽  
Activity Level ◽  
Biologically Active ◽  
Superoxide Dismutase Activity ◽  
Control Group ◽  
Intragastric Administration ◽  
Standard Diet

Aim. To determine the activity of antioxidant enzymes in rat liver tissue on the background of thiamazole hypothyroidism, and also after its correction with iodine-containing organo-mineral complexes. Methods. Studies were conducted on rats, which were divided into four groups: the first group - the control, in animals of the second, third and fourth groups hypothyroidism was induced by daily intragastric administration of thiamazole at a dose 2.5 mg per 100 g body weight for the duration of 3 weeks. Beginning from the 22-day of the experiment the animals of the fourth group for the duration of a month received a biologically active additive in a dose that provides the daily requirement of iodine in rats, while the animals of the third group were on the standard diet of the vivarium. The activity of superoxide dismutase and glutathione peroxidase was determined using a set of reagents «RANSOD Randox» manufactured by «Laboratories Ltd.», the catalase activity was determined by the method of M.A. Korolyuk. Results. Experimental hypothyroidism in rats was characterized by a decrease in the concentrations of free thyroxine, an increase in the content of total triiodothyronine and thyroid-stimulating hormone. In animals treated with tiamazol, the superoxide dismutase activity was 85.6% of the activity level of the control animals, glutathione activity - 77.3% of the level of intact animals. The catalase activity in hypothyroidism decreased significantly - down to 40% of the control level (p ≤0.001). In the liver homogenate of rats, treated for 1 month with «iodine biopolymer» after hypothyroidism induction, the superoxide dismutase activity almost reached the control values and accounted for 95.5% of the activity of intact animals. The activity of glutathione peroxidase and catalase, was even slightly higher than the control values, reaching 115.6 and 112.7% of levels of activity in the control group, respectively (p ≤0,05). At the same time in the animals, which were on a standard diet, the activity of the studied enzymes remained below the control values, with the catalase activity - significantly lower (49.9% of the control, p ≤0,001). Conclusion. The introduction of an iodine-containing biologically active compound on the background of hypothyroidism made it possible to restore the activity of thyroid-dependant antioxidant enzymes, to normalize the functional state of the pituitary-thyroid system and to inhibit the processes of lipid peroxidation in the liver the of animals.

Proteomic Analysis of the Vitreous Body in Proliferative and Non-Proliferative Diabetic Retinopathy

2020 ◽  
Vol 17 ◽  
Author(s):  
Van-An Duong ◽  
Jeeyun Ahn ◽  
Na-Young Han ◽  
Jong-Moon Park ◽  
Jeong-Hun Mok ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Microvascular Complications ◽  
Treatment Options ◽  
Vitreous Body ◽  
Control Group ◽  
Diabetic Patients ◽  
Protein Protein Interaction ◽  
Alpha Beta ◽  
New Treatment

Background: Diabetic Retinopathy (DR), one of the major microvascular complications commonly occurring in diabetic patients, can be classified into Proliferative Diabetic Retinopathy (PDR) and Non-Proliferative Diabetic Retinopathy (NPDR). Currently available therapies are only targeted for later stages of the disease in which some pathologic changes may be irreversible. Thus, there is a need to develop new treatment options for earlier stages of DR through revealing pathological mechanisms of PDR and NPDR. Objective: The purpose of this study was to characterize proteomes of diabetic through quantitative analysis of PDR and NPDR. Methods: Vitreous body was collected from three groups: control (non-diabetes mellitus), NPDR, and PDR. Vitreous proteins were digested to peptide mixtures and analyzed using LC-MS/MS. MaxQuant was used to search against the database and statistical analyses were performed using Perseus. Gene ontology analysis, related-disease identification, and protein-protein interaction were performed using the differential expressed proteins. Results: Twenty proteins were identified as critical in PDR and NPDR. The NPDR group showed different expressions of kininogen-1, serotransferrin, ribonuclease pancreatic, osteopontin, keratin type II cytoskeletal 2 epidermal, and transthyretin. Also, prothrombin, signal transducer and activator of transcription 4, hemoglobin subunit alpha, beta, and delta were particularly up-regulated proteins for PDR group. The up-regulated proteins related to complement and coagulation cascades. Statherin was down-regulated in PDR and NPDR compared with the control group. Transthyretin was the unique protein that increased its abundance in NPDR compared with the PDR and control group. Conclusion: This study confirmed the different expressions of some proteins in PDR and NPDR. Additionally, we revealed uniquely expressed proteins of PDR and NPDR, which would be differential biomarkers: prothrombin, alpha-2-HS-glycoprotein, hemoglobin subunit alpha, beta, and transthyretin.

Immunotherapy and potential predictive biomarkers in the treatment of neuroendocrine neoplasia

2020 ◽  
Author(s):  
Guanghui Xu ◽  
Yuhao Wang ◽  
Hushan Zhang ◽  
Xueke She ◽  
Jianjun Yang
Keyword(s):  
Current Knowledge ◽  
Checkpoint Inhibitors ◽  
Treatment Options ◽  
Predictive Biomarkers ◽  
The Body ◽  
Low Grade ◽  
Ablative Therapies ◽  
Cancer Types ◽  
New Treatment ◽  
Well Differentiated

Neuroendocrine neoplasias (NENs) are a heterogeneous group of rare tumors scattered throughout the body. Surgery, locoregional or ablative therapies as well as maintenance treatments are applied in well-differentiated, low-grade NENs, whereas cytotoxic chemotherapy is usually applied in high-grade neuroendocrine carcinomas. However, treatment options for patients with advanced or metastatic NENs are limited. Immunotherapy has provided new treatment approaches for many cancer types, including neuroendocrine tumors, but predictive biomarkers of immune checkpoint inhibitors (ICIs) in the treatment of NENs have not been fully reported. By reviewing the literature and international congress abstracts, we summarize the current knowledge of ICIs, potential predicative biomarkers in the treatment of NENs, implications and efficacy of ICIs as well as biomarkers for NENs of gastroenteropancreatic system, lung NENs and Merkel cell carcinoma in clinical practice.

scholarly journals Effect of catalase inactivation on levels of inorganic peroxides, superoxide dismutase, glutathione, oxygen consumption and life span in adult houseflies (Musca domestica)

1983 ◽  
Vol 216 (2) ◽  
pp. 503-506 ◽  
Author(s):  
R G Allen ◽  
K J Farmer ◽  
R S Sohal
Keyword(s):  
Superoxide Dismutase ◽  
Oxygen Consumption ◽  
Life Span ◽  
Musca Domestica ◽  
Catalase Activity ◽  
Reduced Glutathione ◽  
Superoxide Dismutase Activity ◽  
Adaptive Responses ◽  
Total Inhibition ◽  
Rate Of Oxygen Consumption

The effects of total inhibition of catalase, induced by 3-amino-1,2,4-triazole, on the adult housefly (Musca domestica) were examined. The lack of catalase activity had no effect on the longevity of the houseflies. Inorganic-peroxide concentration was elevated at younger ages, but declined in older flies. The rate of oxygen consumption by the flies was greatly decreased and the levels of oxidized as well as reduced glutathione were augmented. Superoxide dismutase activity showed a slight increase. This study suggests that loss of catalase activity does not affect survival of houseflies due to adaptive responses.

scholarly journals OPERATION STRESS AND ITS INFLUENCE ON THE SYSTEM CHANGES IN CASE OF ABDOMINAL COMBINED TRAUMA (EXPERIMENTAL RESEARCH)

2018 ◽  
Vol 17 (2) ◽  
pp. 31-36
Author(s):  
V. O. Krylyuk ◽  
V. K. Hrodetsʹkyy ◽  
S. O. Sokolʹnyk ◽  
O. V. Fedosyeyeva ◽  
H. Yu. Tsymbalyuk
Keyword(s):  
Superoxide Dismutase ◽  
Abdominal Cavity ◽  
The Body ◽  
Control Group ◽  
Systemic Response ◽  
Antioxidant Protection ◽  
Operational Stress ◽  
Experimental Animals ◽  
Ischemic Reperfusion ◽  
The Right

The aim of the study was to study the possibility of using a hemostatic biological haemostatic to stop bleeding from the parenchymal organs of the abdominal cavity using the surgical method of Damage control. The experiment was performed on laboratory rats that were divided into three groups: a control group, a group where, after injury, the liver wound was sutured with standard sutures and a group where the wound was plastered with a biological hemostatic agent. In order to determine the operational stress, we studied the enzymatic link of antioxidant protection - the level of catalase and superoxide dismutase. To study the indices in all experimental animals, the following tissues were taken: the lower lobe of the right lung, the proportion of the liver where the wounds were applied, the gastrocnemius muscle below the harness and the right kidney. The control points of the study were 1, 3 and 7 days after injury. During working with experimental animals, they adhered to all ethical norms established by international rules. The effect of operational stress on the enzymatic component of antioxidant protection in the tissues of the kidneys, liver, lungs and muscles was studied in the study of the combined trauma of the abdominal cavity complicated by massive bleeding and ischemic-reperfusion syndrome of the extremity. The changes in the indices of superoxide dismutase and catalase were studied. The following result was obtained: tamponing the liver wound with chitosan was a quick and effective means of temporary bleeding stop. At the same time, the duration of the operation was reduced from 19.8 to 12.5 minutes (by 36.8%) (p <0.05). Also, the level of catalase and superoxide dismutase was significantly lower in the group where the chitosan of the liver injury was used in comparison with the study group where standard stitches were applied. The deviations, which are determined in lung, liver, kidney and muscle tissues in RG-1 are evidence of the depth of their lesion and dysfunction, is a favorable factor for the further development of the triggering mechanisms of the systemic response of the body to inflammation and multiple organ failure. In contrast to the RG-2 rapid development of the systemic response was avoided. We can assume that ischemic-reperfusion syndrome is a stimulating factor in the development of systemic changes, although this statement requires further proof. It is also important to further prospective study of this topic in terms of the following questions: what effect has chitosan on liver tissue with prolonged exposure (more than 2 days) and what effect does additional injection of infusion solutions have as an agent for the treatment of hypovolemic shock.

scholarly journals Effects of Three Types of Massage on Serum Levels of Malondialdehyde, Superoxide Dismutase and Glutathione Peroxidase After One Session of Exhaustive Exercise in Female Futsal Players

2021 ◽  
Vol 10 (4) ◽  
pp. 328-339
Author(s):  
Bahare Heydari ◽  
◽  
Mohsen Ghofrani ◽  
Mohammad Ebrahim Bahram ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Recovery Period ◽  
Serum Levels ◽  
The Body ◽  
Exhaustive Exercise ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Significant Difference

Objective: The production of reactive oxygen species in exercise causes oxidative stress which disturbs the balance of oxidants and antioxidants, causing destructive effects on cells. The present study aims to investigate the effect of three types of massage (Swedish, Russian, Thai) on serum levels of Malondialdehyde (MDA), Glutathione Peroxidase (GPX) and Superoxide Dismutase (SOD) following one session of exhaustive exercise. Methods: This quasi-experimental study was conducted on 48 female futsal players aged 17-22 years in Zahedan, Iran who were selected using a purposive sampling method, and randomly divided into four groups of Swedish massage (Long strokes with pressing and tapping using hands), Russian massage (Medium to high pressure), Thai massage (Pressure to certain parts of the body) and Control. The exercise program was based on Bruce protocol. Serum levels of MDA, GPX and SOD were measured by before and immediately after exercise and after massage. Data analysis was performed using repeated measures ANOVA, considering a significance level of P≤0.05. Results: In all three types of massage, there was a significant decrease in serum level of MDA (0.22±0.08), and a significant increase in GPX (1.84±0.46) and SOD (10.02±2.86) levels after exhaustive (P=0.001). No significant difference was observed in the control group. Conclusion: It seems that Russian, Thai, and Swedish types of massage can affect the serum levels of the MDA (as an oxidative stress marker) and the antioxidant enzymes of GPX and SOD during the post-exercise recovery period.

scholarly journals Influence of melatonin on the kidneys of rats with experimental diabetes mellitus type 2

2020 ◽  
Vol 11 (3) ◽  
pp. 384-391
Author(s):  
A. V. Semenko ◽  
Y. V. Murdasov ◽  
S. V. Kirichenko ◽  
V. I. Zhyliuk ◽  
G. A. Ushakovа
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Molecular Weight ◽  
Superoxide Dismutase ◽  
Experimental Studies ◽  
The Body ◽  
Control Group ◽  
Negative Consequences ◽  
Oxidized Products

Diabetes mellitus is characterized by numerous pathological changes in the body. Under conditions of diabetes, hyperglycemic intoxication of the organism rapidly develops, which in turn leads to an increase of oxidative stress with subsequent disturbance of the anatomical and functional integrity of the components of organisms. Today, the search for the substances that would contribute to the multi-vectoral effect on the negative consequences of diabetes is actively being pursued. Melatonin is one of such substances. In this work, we studied the effect of melatonin on oxidative stress markers (oxidized products content, activities of superoxide dismutase and catalase), the concentration of metabolism end products (creatinine and urea), main ions concentration (potassium and chlorine), and protein content (total protein and electropherogram in polyacrylamide gel), enzymatic activity of gamma-glutamyltrasferase in the cytosolic fraction of rat kidneys under condition of type 2 diabetes mellitus (EDM2). Experimental studies were performed on 18 white adult Wistar rats divided into three groups (control, group with EDM2 and group with EDM2, which were treated with melatonin). The increase of concentration of oxidized products, the activity of catalase and gamma-glutamyltrasferase, creatinine, urea, K+ and Cl– and the decrease of concentration of superoxide dismutase in the rats’ kidneys was noted after development of EDM2. The electrophoretic proteinogram of the cytosolic proteins obtained from the rats’ kidneys showed an increase of content of high-molecular-weight and a decrease of low-molecular-weight proteins. Administration of melatonin in a dose of 10 mg/kg of body weight for 7 days after development of EDM2 restored the studied parameters almost to the control group values. Therefore, the influence of melatonin can prevent chronic development of oxidative stress in kidneys under hyperglycemic intoxication, and lead to normalization of kidney function and the restoration of homeostasis.

Activity of erythrocyte antioxidant enzymes in healthy women depends on age, BMI, physical activity and diet

2020 ◽  
Author(s):  
Elżbieta Cecerska-Heryć ◽  
Klaudia Krauze ◽  
Angelika Szczęśniak ◽  
Aleksandra Goryniak Mikołajczyk ◽  
Natalia Serwin ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Physical Activity ◽  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Antioxidant Enzymes ◽  
Catalase Activity ◽  
Smoking Status ◽  
Superoxide Dismutase Activity ◽  
Antioxidant Systems ◽  
Healthy Women

Abstract Introduction Antioxidant enzymes protect the human body against the harmful effects of oxidative stress. The activity of antioxidant enzymes changes with age, and depends on dietary nutrients such as fats and vitamins, which can have a significant impact on minimizing or exacerbating oxidative stress. Aim Examine the effect of age, BMI, diet, physical activity and smoking status on the activity of erythrocyte antioxidant enzymes catalase, glutathione reductase, glutathione peroxidase glutathione S-transferase, superoxide dismutase and glutathione concentrations in healthy women. Material and methods This study included 98 healthy women aged between 20 and 65 years. All women underwent anthropometric tests: body weight, height, hip and waist circumference. Antioxidant activity in erythrocytes was measured by spectrophotometric methods. Results Catalase activity increased significantly with age (p<0.001), while superoxide dismutase activities and glutathione decreased with age (p =0.008, p =0.023, respectively). Women with a lower BMI (emaciation) had higher superoxide dismutase activity than those in the first degree of obesity (p = 0.009 Conclusions 1. Increased catalase activity with age may be a sign of a large amount of hydrogen peroxide, resulting from poorly functioning antioxidant systems in older age. 2. Decreased superoxide dismutase activity with age may indicate inactivation of this enzyme by excessive hydrogen peroxide, as well as glycation of superoxide dismutase molecules or reactions with lipid peroxidation products, the intensity of which increases with age. 3. The negative correlation between superoxide dismutase activity and BMI index indicates reduced enzymatic activity in obese subjects, despite increased ROS production by adipose tissue.

Sign in / Sign up

Export Citation Format

Share Document