Comparison of fluorescent antibody, bacitracin susceptibility, latex agglutination, coagglutination, and API 20S for identifying group A streptococci

1985 ◽  
Vol 31 (4) ◽  
pp. 335-338
Author(s):  
R. J. Lesher ◽  
A. E. Casiano-Colon
Keyword(s):  
Fluorescent Antibody ◽  
Latex Agglutination ◽  
False Positives ◽  
Fluorescent Antibody Technique ◽  
Group A Streptococci ◽  
Antibody Technique ◽  
Clinical Specimens ◽  
Group A ◽  
Precipitin Test ◽  
Better Than

A total of 200 beta-hemolytic streptococci, isolated from clinical specimens submitted to our laboratory, were identified as group A versus non-A using the fluorescent antibody technique (FA), bacitracin susceptibility (BBL, Difco, and Raven disks), SeroSTAT, Streptex, Phadebact, and the API 20S system. Of the 122 group A isolates, all methods except SeroSTAT and Phadebact yielded 92–99% agreement when compared with the Lancefield precipitin test. Phadebact yielded an 84% agreement and SeroSTAT changed from 83 to 98% after trypsinization. Numerous false positives were obtained and only FA (91%) and API 20S (96%) yielded better than 90% agreement on non-A identification when compared with the Lancefield test. The most false positives were obtained (45%) using the SeroSTAT reagents. Considering accuracy, our data suggests the FA technique to be the method of choice for identifying group A streptococci.

scholarly journals STUDIES ON THE MECHANISM OF THE LONG CHAIN PHENOMENON OF GROUP A STREPTOCOCCI

1963 ◽  
Vol 117 (4) ◽  
pp. 583-594 ◽  
Author(s):  
Jerome J. Hahn ◽  
Roger M. Cole
Keyword(s):  
Specific Antibody ◽  
Fluorescent Antibody ◽  
Antibody Fragments ◽  
Fluorescent Antibody Technique ◽  
Group A Streptococci ◽  
Antibody Technique ◽  
Free Antibody ◽  
Group A ◽  
Long Chain ◽  
Long Chains

The formation and destruction of long chains by growth of Group A streptococci in the presence of type-specific antibody have been studied with the fluorescent antibody technique. Long chain formation has been shown to depend on the presence of free antibody during the growth of the bacteria. Destruction of long chains has been shown to depend on the continued growth and division of the bacteria in the absence of free antibody. Univalent antibody fragments formed by proteolytic digestion of antibody globulin have been shown to have the combining properties of untreated antibody but do not result in the production of long chains. A model involving end-to-end agglutination during growth of Group A streptococci has been presented to explain the mechanism of production of long chains by growth of Group A streptococci in the presence of type-specific antibody.

scholarly journals Rapid grouping of beta-hemolytic streptococci by latex agglutination

1978 ◽  
Vol 8 (3) ◽  
pp. 326-328
Author(s):  
Y A Lue ◽  
I P Howit ◽  
P D Ellner
Keyword(s):  
Fluorescent Antibody ◽  
Latex Agglutination ◽  
Group A Streptococci ◽  
Cross Reactions ◽  
Antibody Staining ◽  
Group A ◽  
Group B ◽  
Fluorescent Antibody Staining

Latex agglutination was compared with fluorescent-antibody staining with group A conjugate and Lancefield precipitation for grouping of beta-hemolytic streptococci. Latex agglutination correctly grouped 98.8% of 82 group A streptococci and more than 95% of 187 group B, C, or G streptococci. Occasional cross-reactions occurred between groups A and C and groups B and G.

scholarly journals GROUP A STREPTOCOCCUS POLYSACCHARIDE: STUDIES ON ITS PREPARATION, CHEMICAL COMPOSITION, AND CELLULAR LOCALIZATION AFTER INTRAVENOUS INJECTION INTO MICE

1952 ◽  
Vol 95 (1) ◽  
pp. 105-118 ◽  
Author(s):  
Willard C. Schmidt
Keyword(s):  
Chemical Composition ◽  
Intravenous Injection ◽  
Cellular Localization ◽  
Group A Streptococcus ◽  
Fluorescent Antibody ◽  
Chemical Properties ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Group A ◽  
Renal Tubular

A method was developed for the extraction of the group A streptococcus polysaccharide employing pepsin digestion of ground streptococcal cells. This method did not result in the isolation of polysaccharide with chemical and physical chemical properties different from those exhibited by preparations extracted with hot formamide. Studies of the chemical composition of this polysaccharide demonstrated it to be composed chiefly of rhamnose and glucosamine monosaccharide units in the approximate ratio of five moles of rhamnose to two moles of glucosamine. The fate of the polysaccharide after intravenous injection into mice was studied using the fluorescent antibody technique. It was found to be rapidly eliminated by the kidney. The presence of the polysaccharide in the renal tubular epithelial cells during the excretory phase was the only evidence of its cellular localization that could be detected under the conditions of these experiments.

Accelerated detection of lymphocytic choriomeningitis virus in diagnostic specimens

1976 ◽  
Vol 22 (11) ◽  
pp. 1662-1663
Author(s):  
Vester J. Lewis ◽  
W. Lanier Thacker ◽  
Shannon H. Mitchell
Keyword(s):  
Fluorescent Antibody ◽  
Lymphocytic Choriomeningitis ◽  
Lymphocytic Choriomeningitis Virus ◽  
Fluorescent Antibody Technique ◽  
Optimal Route ◽  
Antibody Technique ◽  
Clinical Specimens ◽  
The Brain

Lymphocytic choriomeningitis virus could be demonstrated earlier in mice inoculated with clinical specimens if the mice were sacrificed before they appeared sick for examination by the fluorescent antibody technique. In the procedure, the optimal route for inoculation was intracerebral and the best organ tested for staining was the brain.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection
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