scholarly journals Field Application of Fluorescent Antibody Technique for Identification of Group A Streptococcus

1961 ◽  
Vol 76 (8) ◽  
pp. 651 ◽  
Author(s):  
William J. Peeples ◽  
D. W. Spielman ◽  
Max D. Moody
Keyword(s):  
Group A Streptococcus ◽  
Fluorescent Antibody ◽  
Field Application ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Group A

scholarly journals GROUP A STREPTOCOCCUS POLYSACCHARIDE: STUDIES ON ITS PREPARATION, CHEMICAL COMPOSITION, AND CELLULAR LOCALIZATION AFTER INTRAVENOUS INJECTION INTO MICE

1952 ◽  
Vol 95 (1) ◽  
pp. 105-118 ◽  
Author(s):  
Willard C. Schmidt
Keyword(s):  
Chemical Composition ◽  
Intravenous Injection ◽  
Cellular Localization ◽  
Group A Streptococcus ◽  
Fluorescent Antibody ◽  
Chemical Properties ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Group A ◽  
Renal Tubular

A method was developed for the extraction of the group A streptococcus polysaccharide employing pepsin digestion of ground streptococcal cells. This method did not result in the isolation of polysaccharide with chemical and physical chemical properties different from those exhibited by preparations extracted with hot formamide. Studies of the chemical composition of this polysaccharide demonstrated it to be composed chiefly of rhamnose and glucosamine monosaccharide units in the approximate ratio of five moles of rhamnose to two moles of glucosamine. The fate of the polysaccharide after intravenous injection into mice was studied using the fluorescent antibody technique. It was found to be rapidly eliminated by the kidney. The presence of the polysaccharide in the renal tubular epithelial cells during the excretory phase was the only evidence of its cellular localization that could be detected under the conditions of these experiments.

scholarly journals A conglutination test for rapid detection of antibodies against Babesia bigemina

2000 ◽  
Vol 20 (4) ◽  
pp. 161-166 ◽  
Author(s):  
Claudio R. Madruga ◽  
Raul H. Kessler ◽  
Maria A. M. Schenk ◽  
Midori Miguita
Keyword(s):  
Rapid Detection ◽  
Serological Test ◽  
Fluorescent Antibody ◽  
Field Application ◽  
Control Measures ◽  
Babesia Bovis ◽  
Fluorescent Antibody Technique ◽  
Babesia Bigemina ◽  
Antibody Technique ◽  
Epidemiological Surveys

A rapid conglutination test (RCT) with performance comparable to the indirect fluorescent antibody technique (IFAT) was developed to detect antibodies against Babesia bigemina (B. bigemina-RCT). The B. bigemina-RCT is a sensitive, specific, economical, and rapidly performed serological test suitable for field application or minimally equipped laboratories. This test had a sensitivity of 90.9%, and specificity of 97.6%, compared to IFAT, which showed for the same parameters respectively, 98.3% and 99.7%. The early detection of anti- B. bigemina immunoglobulins by RCT in experimental infections was nearly parallel to that of IFAT. Cross reactions were observed with sera from calves experimentally infected with Babesia bovis (1.8%) and with Anaplasma marginale (1.2%). RCT antigen prepared with non parasitized erythrocytes (negative antigen) showed 1.5%, 3.5% and 2.2% of positive reactions with sera from animals experimentally infected with B. bigemina, B. bovis and A. marginale. However, none of the sera from animals of endemic areas for babesia infection resulted in positive reactions with the negative antigen. Considering these results and shelf life over six months, the B. bigemina-RCT could be used for epidemiological surveys and evaluation of control measures against this species of Babesia.

Comparison of fluorescent antibody, bacitracin susceptibility, latex agglutination, coagglutination, and API 20S for identifying group A streptococci

1985 ◽  
Vol 31 (4) ◽  
pp. 335-338
Author(s):  
R. J. Lesher ◽  
A. E. Casiano-Colon
Keyword(s):  
Fluorescent Antibody ◽  
Latex Agglutination ◽  
False Positives ◽  
Fluorescent Antibody Technique ◽  
Group A Streptococci ◽  
Antibody Technique ◽  
Clinical Specimens ◽  
Group A ◽  
Precipitin Test ◽  
Better Than

A total of 200 beta-hemolytic streptococci, isolated from clinical specimens submitted to our laboratory, were identified as group A versus non-A using the fluorescent antibody technique (FA), bacitracin susceptibility (BBL, Difco, and Raven disks), SeroSTAT, Streptex, Phadebact, and the API 20S system. Of the 122 group A isolates, all methods except SeroSTAT and Phadebact yielded 92–99% agreement when compared with the Lancefield precipitin test. Phadebact yielded an 84% agreement and SeroSTAT changed from 83 to 98% after trypsinization. Numerous false positives were obtained and only FA (91%) and API 20S (96%) yielded better than 90% agreement on non-A identification when compared with the Lancefield test. The most false positives were obtained (45%) using the SeroSTAT reagents. Considering accuracy, our data suggests the FA technique to be the method of choice for identifying group A streptococci.

scholarly journals STUDIES ON THE MECHANISM OF THE LONG CHAIN PHENOMENON OF GROUP A STREPTOCOCCI

1963 ◽  
Vol 117 (4) ◽  
pp. 583-594 ◽  
Author(s):  
Jerome J. Hahn ◽  
Roger M. Cole
Keyword(s):  
Specific Antibody ◽  
Fluorescent Antibody ◽  
Antibody Fragments ◽  
Fluorescent Antibody Technique ◽  
Group A Streptococci ◽  
Antibody Technique ◽  
Free Antibody ◽  
Group A ◽  
Long Chain ◽  
Long Chains

The formation and destruction of long chains by growth of Group A streptococci in the presence of type-specific antibody have been studied with the fluorescent antibody technique. Long chain formation has been shown to depend on the presence of free antibody during the growth of the bacteria. Destruction of long chains has been shown to depend on the continued growth and division of the bacteria in the absence of free antibody. Univalent antibody fragments formed by proteolytic digestion of antibody globulin have been shown to have the combining properties of untreated antibody but do not result in the production of long chains. A model involving end-to-end agglutination during growth of Group A streptococci has been presented to explain the mechanism of production of long chains by growth of Group A streptococci in the presence of type-specific antibody.

Rapid quantification and in situ detection of nitrifying bacteria in biofilms by monoclonal antibody method

2000 ◽  
Vol 41 (4-5) ◽  
pp. 301-308 ◽  
Author(s):  
N. Noda ◽  
H. Ikuta ◽  
Y. Ebie ◽  
A. Hirata ◽  
S. Tsuneda ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Fluorescent Antibody ◽  
Nitrifying Bacteria ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Antibody Method ◽  
In Situ Detection ◽  
Rapid Quantification

Fluorescent antibody technique by the monoclonal antibody method is very useful and helpful for the rapid quantification and in situ detection of the specific bacteria like nitrifiers in a mixed baxterial habitat such as a biofilm. In this study, twelve monoclonal antibodies against Nitrosomonas europaea (IFO14298) and sixteen against Nitrobacter winogradskyi (IFO14297) were raised from splenocytes of mice (BALB/c). It was found that these antibodies exhibited little cross reactivity against various kinds of heterotrophic bacteria. The direct cell count method using monoclonal antibodies could exactly detect and rapidly quantify N. europaea and N. winogradskyi. Moreover, the distribution of N. europaea and N. winogradskyi in a biofilm could be examined by in situ fluorescent antibody technique. It was shown that most of N. winogradskyi existed near the surface part and most of N. europaea existed at the inner part of the polyethylene glycol (PEG) gel pellet, which had entrapped activated sludge and used in a landfill leachate treatment reactor. It was suggested that this monoclonal antibody method was utilized for estimating and controlling the population of nitrifying bacteria as a quick and favorable tool.

Rubella Antibodies in Human Serum: Detection by the Indirect Fluorescent-Antibody Technique

Science ◽  
1964 ◽  
Vol 145 (3635) ◽  
pp. 943-945 ◽  
Author(s):  
G. C. Brown ◽  
H. F. Maassab ◽  
J. A. Veronelli ◽  
T. J. Francis
Keyword(s):  
Human Serum ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Serum Detection

scholarly journals LOCALIZATION OF UROMUCOID IN HUMAN KIDNEY AND IN SECTIONS OF HUMAN KIDNEY STONE WITH THE FLUORESCENT ANTIBODY TECHNIQUE

1965 ◽  
Vol 13 (3) ◽  
pp. 155-160 ◽  
Author(s):  
H. J. KEUTEL
Keyword(s):  
Kidney Stones ◽  
Kidney Stone ◽  
Fluorescent Antibody ◽  
Human Kidney ◽  
Fluorescent Antibody Technique ◽  
Renal Tubules ◽  
Antibody Technique ◽  
The Matrix ◽  
Normal Human ◽  
The Common

Fluorescent labeled antibodies were used for the demonstration of uromucoid. This urine specific mucoprotein is demonstrably present only in the epithelial cells of the proximal segments of the normal human renal tubules and in the matrix of human kidney stones of all the common crystalline compositions.

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