Sclerotia of the Gasteromycete Pisolithus tinctorius

1980 ◽  
Vol 26 (12) ◽  
pp. 1505-1507 ◽  
Author(s):  
John J. Dennis
Keyword(s):  
Mycorrhizal Fungus ◽  
Root Systems ◽  
Pisolithus Tinctorius ◽  
Pine Seedlings

Hyphal masses, morphologically identified as the sclerotia of Pisolithus tinctorius, were found associated with root systems of containerized pine seedlings inoculated with this mycorrhizal fungus. The sclerotia are described and the results and method used for isolation are reported.

Influence of Fertilization and Ectomycorrhizae on Loblolly Pine Growth and Susceptibility to Fusiform Rust

1983 ◽  
Vol 7 (2) ◽  
pp. 101-103 ◽  
Author(s):  
H. R. Powers ◽  
S. J. Rowan
Keyword(s):  
Loblolly Pine ◽  
Cultural Practices ◽  
Mycorrhizal Fungus ◽  
Height Growth ◽  
Pisolithus Tinctorius ◽  
Fusiform Rust ◽  
Infected Tree ◽  
Pine Seedlings ◽  
Susceptible Control ◽  
Rust Severity

Abstract The application of fertilizer and the mycorrhizal fungus, Pisolithus tinctorius, did not increase the height growth of loblolly pine seedlings during their first four years of growth. Fertilization, however, did increase the overall number of fusiform rust galls per infected tree. This increase was highly significant on the susceptible control seedlings, but was not significant on resistant Livingston Parish seedlings. This indicates that cultural practices such as fertilization can be applied to rust-resistant seedlings, such as the Livingston Parish seed source, without significantly increasing rust severity.

scholarly journals The effect of ectomycorrhizal fungi and bacteria on pine seedlings

2014 ◽  
Vol 33 (1) ◽  
pp. 25-36 ◽  
Author(s):  
Hanna Dahm ◽  
Edward Strzelczyk ◽  
Agnieszka Ciesielska ◽  
Katarzyna Redlak
Keyword(s):  
Ectomycorrhizal Fungi ◽  
Fungal Biomass ◽  
Azospirillum Brasilense ◽  
Mycorrhizal Fungus ◽  
Pisolithus Tinctorius ◽  
Shoot Ratio ◽  
Root Shoot Ratio ◽  
Pine Seedlings ◽  
Hebeloma Crustuliniforme ◽  
The Relationship

The effect of ecomycorrhizal fungi (<i>Hebelon crustuliniforme</i>(Bull.: Fr.) Quél. 5392 and <i> Pisolithus tinctorius</i> (Pers.) Coker et Couch 5335) and bacteria (<i>Bacillus polymyxa</i> and <i>Azospirillum brasilense</i>). associated with mycorrhizas on the growth of pine seedligs was investigated. In addition the influence of bacteria on fungal biomass production and the relationship between ectomycorrhizal fungi and fungi pathogenic to root of pine seedlings were determined. In general, the shoot/root ratio was higher in plants inoculated with <i>Hebeloma crustuliniforme</i> and bacteria than in the control seedlings (grown only under sterile conditions). In non-sterile substrate the root/shoot ratio of the mycorrhizal seedlings was lower as compared to the control. Similar phenomenon was noted in plants inoculated with the mycorrhizal fungus <i>Pisolithus tinetorius</i>. The bacteria used as well as the time of introduction of these organisms into the cultures of mycorrhiza fungi affected the production of fungal biomass. <i>Hebeloma crustuliniforme</i> and <i>Pisolithus tinctorius</i> inhibited the growth of <i>Rizoctonia solani</i> and <i>Fusarium oxysporum</i> fungi pathogenic to pine seedlings.

Influence of Tylenchorhynchus ewingi on growth of loblolly pine seedlings, and host suitability of legumes and small grains

Nematology ◽  
2012 ◽  
Vol 14 (4) ◽  
pp. 417-425 ◽  
Author(s):  
Stephen W. Fraedrich ◽  
Michelle M. Cram ◽  
Zafar A. Handoo ◽  
Stanley J. Zarnoch
Keyword(s):  
Pearl Millet ◽  
Loblolly Pine ◽  
Cover Crops ◽  
Root Systems ◽  
Forest Tree ◽  
Initial Population ◽  
Severe Damage ◽  
Pine Seedlings ◽  
Small Grains ◽  
Production Losses

Tylenchorhynchus ewingi, a stunt nematode, causes severe injury to slash pine seedlings and has been recently associated with stunting and chlorosis of loblolly pine seedlings at some forest tree nurseries in southern USA. Experiments confirmed that loblolly pine is a host for T. ewingi, and that the nematode is capable of causing severe damage to root systems. Initial population densities as low as 60 nematodes (100 cm3 soil)−1 were sufficient to damage the root systems of loblolly pine seedlings. Populations of T. ewingi increased on pine from two- to 16-fold, depending on the initial population density. Evaluations of various cover crops used in southern forest tree nurseries indicated that legumes, rye and several varieties of sorghum were excellent hosts for T. ewingi. Other small grains such as ryegrass, oats and wheat were poorer hosts. A cultivar of pearl millet was a non-host for T. ewingi, and a cultivar of brown top millet appeared to be either a very poor host or a non-host. Nurseries that have seedling production losses caused by T. ewingi should consider rotating with non-host cover crops such as pearl millet or leaving fields fallow as part of their pest management programme.

Inoculation of Nursery Seedbeds with Pisolithus Tinctorius Spores Mixed with Hydromulch Increases Ectomycorrhizae and Growth of Loblolly Pines

1979 ◽  
Vol 3 (4) ◽  
pp. 175-178 ◽  
Author(s):  
Donald H. Marx ◽  
John G. Mexal ◽  
William G. Morris
Keyword(s):  
Loblolly Pine ◽  
Pisolithus Tinctorius ◽  
Pine Seedlings ◽  
Loblolly Pines

Abstract Different methods of introducing basidiospores (4/5 oz. spores/100 linear ft. of nursery bed) of Pisolithus tinctorius into fumigated soil at Weyerhaeuser's nursery in Oklahoma were tested to determine their effectiveness in forming ectomycorrhizae on loblolly pine seedlings. Two of five methods proved significantly effective. Nearly three-fourths of seedlings treated with spores mixed in hydromulch and applied after sowing formed Pisolithus ectomycorrhizae. The result was 25 percent larger seedlings and 15 percent fewer culls. In plots where spores were dusted onto the soil at sowing, one-third of the seedlings formed Pisolithus ectomycorrhizae, resulting in 12 percent larger seedlings and 13 percent fewer culls.

Nitrogen source effects on Al toxicity in nonmycorrhizal and mycorrhizal pitch pine (Pinus rigida) seedlings. II. Nitrate reduction and NO3− uptake

1990 ◽  
Vol 68 (12) ◽  
pp. 2653-2659 ◽  
Author(s):  
Jonathan R. Cumming
Keyword(s):  
Nitrogen Source ◽  
Nitrate Reduction ◽  
Al Toxicity ◽  
Pisolithus Tinctorius ◽  
Sand Culture ◽  
Root Tips ◽  
Pinus Rigida ◽  
Pine Seedlings ◽  
Pitch Pine ◽  
Nr Activity

Nitrogen source utilization, mediated by the ectomycorrhizal symbiont Pisolithus tinctorius, may modulate Al toxicity in pitch pine (Pinus rigida) seedlings. Nitrate reduction, occurring primarily in the roots of pitch pine seedlings, represents a critical metabolic pathway that may be directly sensitive to Al or indirectly affected by changes in NO3− availability associated with Al exposure or mycorrhizal infection. To investigate these possibilities, pitch pine seedlings were grown in sand culture with NO3−, NH4NO3, or NH4+ and exposed to 0 or 200 μM Al for 6 weeks. Foliar N concentrations, root nitrate reductase (NR) activity, and Al inhibition of NR activity were highly dependent on the proportion of NO3− of the nutrient solution. The association of Pisolithus tinctorius with seedling roots reduced both root and foliar NR activity compared with non-inoculated controls, suggesting that NO3− uptake and translocation to foliage was reduced by the symbiont. This was confirmed by using 36ClO3− to measure unidirectional plasma membrane NO3− fluxes. Mycorrhizal root tips absorbed 50% less NO3− than nonmycorrhizal root tips. Preferential use of NH4+ by ectomycorrhizal roots may thus result in reduced movement of Al into root tissue and amelioration of Al toxicity. Key words: Pinus rigida, ectomycorrhizae, aluminum toxicity, nitrogen source.

TRANSLOCATION OF THE PRODUCTS OF PHOTOSYNTHESIS TO ROOTS OF PINE SEEDLINGS

1962 ◽  
Vol 40 (8) ◽  
pp. 1125-1135 ◽  
Author(s):  
T. Shiroya ◽  
G. R. Lister ◽  
G. Krotkov ◽  
C. D. Nelson ◽  
V. Slankis
Keyword(s):  
Amino Acid ◽  
Pinus Strobus ◽  
Root Systems ◽  
Insoluble Residue ◽  
Artificial Light ◽  
Closed Chamber ◽  
Full Sunlight ◽  
Pine Seedlings ◽  
Artificial Illumination ◽  
Soluble Fractions

Pinus strobus or P. resinosa seedlings, 2 or 3 years old, were illuminated in a closed chamber for 1 hour in the presence of C14O2. This was followed by various periods up to 24 hours under different conditions of light and darkness. Then each seedling was divided into its shoot, stem, and roots, and these were extracted separately with 80% ethanol. The extracts were resolved first on resins into sugar, amino acid, and organic acid fractions and then resolved further by paper chromatography. The C14 content of various fractions and of the eluted compounds was determined by plating and counting their aliquots. Ethanol-insoluble residue was oxidized and counted as BaC14O3.Eight hours after administration of the C14O2, 91 to 94% of the total C14 was found in the ethanol-soluble fractions of shoot, stem, or root. In shoots sugars were found to represent more than 95% of the ethanol-soluble photosynthate, with sucrose forming three-quarters of it. In stem and roots sucrose represented from 75 to 94% of the translocated photosynthate. Raffinose, glucose, and fructose were present in both stem and root.Seedlings with poorly developed root systems translocated less photosynthate to roots than those with good roots. Seedlings, which prior to the experiments were grown in full sunlight or 2500 ft-c artificial illumination translocated more photosynthate to roots than those grown in 6% of full sunlight or 250 ft-c artificial light. Stronger light during translocation itself also had a slight stimulatory effect.Seedlings, which were illuminated in the presence of C14O2 for 1 hour and then retained in a closed chamber for a further period of 7 hours, translocated a larger fraction of absorbed C14 to their roots than comparable seedlings transferred to air after feeding C14O2.

scholarly journals Productivity of seedlings of Scots pine on alluvial sands of natural and man-made origin

2021 ◽  
pp. 88-94
Author(s):  
F.M Brovko ◽  
D.F Brovko ◽  
O.F Brovko ◽  
V.Yu Yukhnovskyi
Keyword(s):  
Water Content ◽  
Scots Pine ◽  
Root Systems ◽  
Pine Plantations ◽  
Individual Growth ◽  
Aboveground Phytomass ◽  
Water Properties ◽  
Pine Seedlings ◽  
Small Roots ◽  
Quantitative Indicators

Purpose. To find out quantitative physical and water indicators for which there are significant changes in forest vegetation properties in alluvial sands, as well as to trace their impact on the formation of pine seedling root systems and the accumulation of aboveground phytomass in their plantations. Methodology. The chemical properties of sandy soils were determined taking into account the current requirements of ISO, and their physical and water properties using volumetric cylinders, followed by the calculation of their density, porosity, as well as the coefficients of water content and aeration. The root population of the upper meter layer of sands was determined by the method of monoliths, and plant productivity was assessed by phytomass of medium model trees (7-year-old seedlings, plots 14) and by biometric indicators (22-year-old seedlings, plots 59). Findings. It was found that on alluvial sands with a density of 1.501.66 g × cm-3 in their upper meter thickness, 7-year-old seedlings of Scots pine form a superficial root system (1341.8 g × m-2), which provides accumulation of 2558 kg × ha-1 of aboveground phytomass in seedlings. As the density of sands increases, the production of seedling phytomass decreases. In the case of an increase in density by 14% (1.521.72 g × cm-3), there is a decrease in the mass of roots, in a meter-thick layer of sand (by 53.4%) and aboveground phytomass (by 36%). An increase in the density of sands by 510% with its maximum values (1.741.79 g × cm-3) in a 1030 cm layer causes a decrease in the mass of pine roots by 64.1%. The roots of pine seedlings, for such a density of sand, are not able to inhabit the inter-row space, as indicated by their content in the upper 20-cm layer of sand (2% of the mass of small roots recorded in a one-meter thickness). The phytomass of aboveground organs decreased by 81%, and the seedlings themselves were marked by dwarf growth (were grown by V class of productivity). On sands covered with humus mass of zonal soils, the one meter thickness contained fewer (by 51.4%) pine roots (482.8 g × m-2) than on the control. The share of small roots was smaller (by 61.5%) and that of coarse roots was higher (by 21.5%). Losses of aboveground phytomass per unit area in pine seedlings growing under such conditions can reach 31%, due to the compaction of sands at a depth of 2550 cm (1.671.72 g × cm-3) when they are covered by humus mass and row spacing are overgrown with herbaceous plants (root mass in 60-cm profile 3147 g × m-2) in the phase of their individual growth. Originality. Quantitative indicators of density, porosity and coefficients of water content and aeration of alluvial sands of natural and man-made origin are shown for which the seedlings of Scots pine feature delay in the formation of full-fledged root systems of the surface type, which is reflected in a decrease in the productivity of pine plantations cultivated on the sands, up to the visual manifestation of their dwarf growth. Practical value. The quantitative indicators of their physical and water properties obtained for alluvial sands explain the changes occurring in the structure of the root systems of Scots pine seedlings and the productivity of their aboveground organs. Maintaining the density of sands in the range of 1.501.66 g × cm-3 will allow growing pine seedlings on sands without covering their surface with humus mass, and no-till pre-planting loosening of sands in the rows of future crops allows ensuring the cultivation of multifunctional pine plantations.

The Effects of Benomyl and Pisolithus Tinctorius Ectomycorrhizae on Survival and Growth of Longleaf Pine Seedlings

1981 ◽  
Vol 5 (4) ◽  
pp. 189-195 ◽  
Author(s):  
Albert G. Kais ◽  
Glenn A. Snow ◽  
Donald H. Marx
Keyword(s):  
Longleaf Pine ◽  
Pinus Palustris ◽  
Height Growth ◽  
Pisolithus Tinctorius ◽  
Brown Spot ◽  
Spot Disease ◽  
Brown Spot Disease ◽  
Pine Seedlings ◽  
Collar Diameter ◽  
Survival And Growth

Abstract Benomyl applied to roots of longleaf pine (Pinus palustris Mill.) seedlings at planting significantly reduced brown-spot disease and increased survival, root collar diameter, and early height growth on two sites in Mississippi. Seedlings with half or more of all ectomycorrhizae formed by Pisolithus tinctorius (Pers.) Coker and Couch in the nursery had significantly better survival and growth; Pisolithus ectomycorrhizae did not appreciably affect brown-spot disease. The benefits of benomyl and Pisolithus ectomycorrhizae were most obvious when combined. More than 75 percent of seedlings treated with benomyl and with more than half of all ectomycorrhizae formed by Pisolithus initiated height growth after 3 years. Forty-seven percent of seedlings with only Thelephora terrestris ectomycorrhizae and without benomyl exhibited height growth. The combined use of benomyl to control brown-spot disease and Pisolithus ectomycorrhizae to stimulate early height growth may overcome the major handicaps that have limited artificial regeneration of longleaf pine in the South.

Converting Rhododendron-Laurel Thickets to White Pine with Picloram and Mycorrhizae-Inoculated Seedlings

1984 ◽  
Vol 8 (3) ◽  
pp. 163-168 ◽  
Author(s):  
Daniel G. Neary ◽  
James E. Douglass ◽  
John L. Ruehle ◽  
Walter Fox
Keyword(s):  
North Carolina ◽  
Seedling Survival ◽  
Mycorrhizal Fungus ◽  
Height Growth ◽  
White Pine ◽  
Basal Diameter ◽  
Kalmia Latifolia ◽  
Pine Seedlings ◽  
Rhododendron Maximum ◽  
Pellet Formulation

Abstract A ridge site in the Appalachian highlands of North Carolina was prepared for planting container-grown white pine (Pinus strobus L.) seedlings by treatment with herbicide. A pellet formulation of picloram (4-amino-3,5,6-trichloropicolinic acid) containing 10% acid equivalent was applied in May 1978. Control of rhododendron (Rhododendron maximum L.), laurel (Kalmia latifolia L.), and other hardwood vegetation was sufficient to allow pine establishment. White pine survival was 96% 18 months after planting. Inoculation of seedlings while still in the greenhouse with the mycorrhizal fungus Pisolithus tinctorius (Pers.) Coker and Couch did not significantly affect seedling survival, total height, seasonal height growth, or basal diameter in the field. Height growth the second growing season after planting 6-month, container-grown stock averaged 5.1 to 7.5 inches. Eighteen months after planting, total seedling height averaged 13.3 inches, with the tallest exceeding 29.1 inches. Both height and diameter growth of white pine seedlings were inversely related to the degree of shading from remaining vegetation.

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