Composition of Methanospirillum hungatii GP1 during growth on different media

1980 ◽  
Vol 26 (5) ◽  
pp. 577-582 ◽  
Author(s):  
C. Breuil ◽  
G. B. Patel
Keyword(s):  
Protein Content ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Synthetic Medium ◽  
Density Measurement ◽  
Dry Weight ◽  
Weight Basis ◽  
Total Carbohydrate ◽  
Mineral Salts

Growth of Methanospirillum hungatii GP1 as determined by optical density measurement was comparable to growth assessed by cell dry weight, ribonucleic acid content, and deoxyribonucleic acid content. Cultivation of M. hungatii on synthetic medium containing mineral salts, vitamins, and acetic acid indicated that, on a dry weight basis, cell constituents such as protein (71%), ribonucleic acid (15.8%), deoxyribonucleic acid (1.6%), and total carbohydrate (3.2%) did not vary significantly with the growth phase. Cells grown in the synthetic medium supplemented with yeast extract and tryptone had slightly higher protein content (76%), but the concentrations of the other cell constituents were similar and did not fluctuate much during growth. Nitrogen limiting growth resulted in somewhat lower ribonucleic acid content as well as slightly higher protein content than that in cells grown in nonlimiting medium. Methanospirillum hungatii did not accumulate any of the commonly known reserve materials under nitrogen or carbon and hydrogen limiting growth.

Aspects of metabolic development in an illuminated synchronous culture of Scenedesmus obtusiusculus

1973 ◽  
Vol 51 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Gaurangakumar Das
Keyword(s):  
Growth Phase ◽  
Ribonucleic Acid ◽  
Culture Medium ◽  
Deoxyribonucleic Acid ◽  
Dry Weight ◽  
Light Period ◽  
Weight Basis ◽  
Lipid Phosphorus ◽  
Acid Phosphate ◽  
Cellular Development

Developing synchronous cells of Scenedesmus obtusiusculus Chod. were investigated during the 15-h light period of the 24-h life cycle. Measurements were made of photosynthesis and of the chlorophyll, carotene, nucleic acid, and protein content, including intracellular phosphorus.Photosynthetic oxygen evolution (μmol/mg chlorophyll) increased during the early growing stage and declined gradually in the older cells. Chlorophyll, carotene, protein, ribonucleic acid, and ribonucleic acid phosphate of these cells increased during the growth phase (dry weight basis) and declined in the sporulating phase, whereas the deoxyribonucleic acid and deoxyribonucleic phosphate decreased during the growth phase. Deoxyribonucleic acid increased slightly in the sporulating phase, but deoxyribonucleic acid phosphate remained relatively constant during this period. When calculated on a per cell basis, ribonucleic acid and ribonucleic acid phosphate increased steadily throughout the period of observation, whereas deoxyribonucleic acid and deoxyribonucleic acid phosphate remained constant during the first 5 h and then increased gradually. Analysis of the intracellular distribution of phosphorus(dry weight basis) revealed that methanol-soluble (lipid) phosphorus decreased steadily with cell growth, whereas the content of acid-soluble phosphorus and ethanol–ether-soluble (lipid) phosphorus fluctuated markedly. The pH of the culture medium increased concomitantly with cellular development during the light period.

SPECIES DIFFERENCES IN THE DEOXYRIBONUCLEIC AND RIBONUCLEIC ACID CONTENTS OF LIVERS OF NON-PREGNANT AND PREGNANT MICE, GUINEA-PIGS AND CATS

1953 ◽  
Vol 9 (1) ◽  
pp. 45-51 ◽  
Author(s):  
ROSA M. CAMPBELL ◽  
H. W. KOSTERLITZ
Keyword(s):  
Guinea Pig ◽  
Protein Content ◽  
Liver Cell ◽  
Ribonucleic Acid ◽  
Guinea Pigs ◽  
Deoxyribonucleic Acid ◽  
Species Differences ◽  
Liver Cells ◽  
Maternal Liver ◽  
Pregnant Mice

1. The protein content of liver cells is almost independent of the size of the animal (mice, cats and previous results on rats, Campbell & Kosterlitz [1949]), and varies with the amount of protein eaten. 2. As has already been shown for rats, the ribonucleic acid ('RNA') content of the liver cells of non-pregnant mice, guinea-pigs and cats varies directly with the protein content of the cells. For a given protein content the mouse and rat have more RNA than the guinea-pig and cat. 3. During pregnancy there is a rise of the deoxyribonucleic acid ('DNA') content of the livers and in the protein content of the liver cells of mice (and rats), but not of guinea-pigs. 4. An excess of RNA over that predicted from the protein content of the liver cell has previously been found for the rat during pregnancy, and ascribed to the action of a placental factor on the maternal liver. A similar excess of RNA has now been observed in the mouse and, to a less extent, in the guinea-pig. It appears to be absent in the cat. 5. Possible causes of some of these species differences are considered.

Changes in the nucleic acid content of the uterus of sheep during involution following ovariectomy and following oestrogenic stimulation

1976 ◽  
Vol 27 (5) ◽  
pp. 669 ◽  
Author(s):  
DA Little ◽  
LJ Lambourne
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Total Content ◽  
Mean Value ◽  
Nucleic Acid Content ◽  
Uterine Tissue ◽  
Rna Concentration

The concentrations of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), in ovine uterine tissue were studied to determine whether the ratio RNA/DNA might be used in the bioassay of oestrogenic activity. As the uterus decreased in size with time after ovariectomy, the concentration of DNA increased markedly, but the total content of DNA in the uterus remained constant (mean value 342 ? 8 (SE) mg). The RNA concentration of the tissue decreased during involution, with the result that a highly significant reduction of 33% in the RNA/DNA ratio occurred within 2 weeks of ovariectomy. The ratio decreased further with time. Other ewes were treated for 3 days with diethylstilboestrol dipropionate (10 µg/day), commencing 2 weeks after ovariectomy; the stage of the oestrous cycle at which the ovaries were removed had no effect on the response to the oestrogen. Changes in the nucleic acid concentrations in the uteri of these ewes, and the results obtained during involution, indicate that the RNA/DNA ratio of uterine tissue in ovariectomized ewes has potential as a basis for the bioassay of materials of low oestrogenic potency.

THE EFFECT OF LARGE DOSES OF THIOUREA ON THE COMPOSITION OF THE LIVER AND URINE OF RATS

1956 ◽  
Vol 13 (2) ◽  
pp. 141-149 ◽  
Author(s):  
F. DOLJANSKI ◽  
Z. ESHKOL ◽  
D. GIVOL ◽  
E. KAUFMANN ◽  
E. MARGOLIASH
Keyword(s):  
Uric Acid ◽  
Urinary Excretion ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Catalase Activity ◽  
Deoxyribonucleic Acid ◽  
Liver Weight ◽  
Liver Glycogen ◽  
Chemical Changes ◽  
Thiourea Treatment

SUMMARY Following the observation that large daily doses of thiourea induced numerous mitoses in the liver of rats [Rachmilewitz, Rosin & Doljanski, 1950], the concomitant chemical changes in the liver and urine of thiourea-treated rats were investigated. It was found that: (1) The mitogenetic reaction, which occurred on the 3rd day of treatment with the drug, was preceded by (a) a decrease in liver catalase activity to 30–50% of the normal within 12 hr; (b) an increase in total liver weight and concentration of liver ribonucleic acid within 2 days. The enlarged liver was normal with respect to protein, lipid, water and deoxyribonucleic acid content. (2) The concentration of liver glycogen decreased markedly on the 1st day of thiourea treatment but returned to normal by the 2nd or 3rd day. (3) During the period of treatment with thiourea there was an increase in the urinary excretion of uric acid, allantoin, and phosphate, as well as glucosuria and polyuria. There was no change in the excretion of urea and total sulphate. (4) All these changes, both in the liver and the urine, were fully reversible on withdrawing the drug.

scholarly journals The Effect of Lighted Incubation on Ribonucleic Acid, Deoxyribonucleic Acid, and Protein Content of Chick Embryos

1981 ◽  
Vol 60 (5) ◽  
pp. 1089-1091 ◽  
Author(s):  
MARILYN A. COLEMAN ◽  
ROBERT C. SMITH ◽  
GAYNER R. McDANIEL
Keyword(s):  
Protein Content ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Chick Embryos

scholarly journals THE DEVELOPMENT IN VITRO OF PARTICLES FROM CYTOPLASM

1958 ◽  
Vol 107 (5) ◽  
pp. 769-782 ◽  
Author(s):  
Eric L. Nelson
Keyword(s):  
Deoxyribonucleic Acid ◽  
Dry Weight ◽  
Weight Basis ◽  
Spherical Particles ◽  
Red Cell ◽  
Practical Applications ◽  
Unknown Factor ◽  
Development In Vitro ◽  
Ph Range

Spherical particles, 1 to 10 microns in diameter, resulted from the incubation at 37°C. of distilled water lysates of erythrocytes with deoxyribonucleic acid (DNA). The particles consisted of 88 per cent hemoglobin and 12 per cent DNA (dry weight basis). An unknown factor, presumably an enzyme, present only in fresh red cell lysates, was required for particle development. Particle size was a function of the pH of the reaction mixture. The pH range was 4.8–5.8. It was possible to trap extraneous proteins and polysaccharides in pockets within the hemoglobin particles during their development to the exclusion of some of the hemoglobin. The amount of any one substance so trapped was proportional to its concentration in the reaction mixture. Some practical applications of the particles, as a means for making particulate various soluble substances (enzymes, antigens, antibiotics), are suggested.

The physiology of growth in Subterranean clover. II. The dynamics of leaf growth

1970 ◽  
Vol 18 (2) ◽  
pp. 149 ◽  
Author(s):  
RF Williams ◽  
. Rijven.A.H.G.C
Keyword(s):  
Cell Wall ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Vascular System ◽  
Leaf Growth ◽  
Subterranean Clover ◽  
Dry Weight ◽  
Protein Nitrogen ◽  
Wall Materials ◽  
Cell Wall Materials

This study extends the account of the development of the fourth leaf of subterranean clover. In particular, the four leaf constituents deoxyribonucleic acid, ribonucleic acid, proteins, and cell wall materials are expressed on whole leaf and cell bases. Form change in the leaf is illustrated with perspective elevations for some early stages. Cell number increases exponentially until day 13, with a mean generation time of 18 hr. The final number per leaf is c. 6.5 million, and deoxyribonucleic acid phosphorus is estimated as 0.197 pg per cell. The leaf is highly succulent, with a maximum water content of 478 % dry weight. Soluble constituents are also high, with a maximum of 39 % of the dry matter. The absolute amounts of ribonucleic acid phosphorus and protein nitrogen are maximal on days 19 and 25 respectively, with losses of about 60% by day 36. Cell wall materials increase from 8 to 57 % of the residual dry weight during a period of 4 days prior to emergence. This is shown to be due to the concurrent differentiation of the vascular system and the growth of 3000 leaf hairs. Changes on a per cell basis are recorded, and the concept of relative growth rate is used to extend the analysis of the data. Rates of production of one constituent per unit of another are also presented and discussed.

scholarly journals Adaptación y calidad tecnológica de la variedad de frijol Negro Tacana.

2016 ◽  
Vol 7 (1) ◽  
pp. 26 ◽  
Author(s):  
Ernesto López ◽  
Noé Becerra ◽  
Octavio Cano ◽  
Dora Zaleta ◽  
Jorge Acosta
Keyword(s):  
Protein Content ◽  
Dry Weight ◽  
Humid Tropics ◽  
Weight Basis ◽  
Fiber Content ◽  
Experimental Conditions ◽  
Yield Trial ◽  
Wide Adaptation ◽  
Stable Yield

In this paper the results obtained with a uniform yield trial including 22 bean genotypes grown in three states of Mexico are presented. Under experimental conditions, bred line DOR 390 resulted outstanding for its wide adaptation, high and stable yield (1041 kg/ha, bi=1.0 Sd2i=0) Under farmer’s conditions, DOR 390 (1214 kg/ha) was superior to commercial cvs, Negro Cotaxtla 91 (1142 kg/ha) and Jamapa (867 kg/ha); at neight locations of the humid tropics in the States of Tamaulipas, Veracruz and Chiapas, DOR 390 proved to be highly resistant in comparison to Negro Huasteco 81. Comparing Dor 390 to bred cvs. and landraces of the same commercial class, tropical black, Dor 390 showed and average protein content of 25%, on a dry weight basis, and the highest fiber content of the seven genotypes evaluated. DOR 390 was registered as a new cultivar in 1993, and made available to the farmers of the humid tropics in 1994, under the name of “Negro Tacana”.

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