Changes in the Dry Weight and the Deoxyribonucleic Acid Content of Proteus vulgaris caused by Penicillin

Nature ◽  
1958 ◽  
Vol 181 (4625) ◽  
pp. 1740-1742 ◽  
Author(s):  
M. V. NERMUT ◽  
V. DRÁŠIL
1980 ◽  
Vol 26 (5) ◽  
pp. 577-582 ◽  
Author(s):  
C. Breuil ◽  
G. B. Patel

Growth of Methanospirillum hungatii GP1 as determined by optical density measurement was comparable to growth assessed by cell dry weight, ribonucleic acid content, and deoxyribonucleic acid content. Cultivation of M. hungatii on synthetic medium containing mineral salts, vitamins, and acetic acid indicated that, on a dry weight basis, cell constituents such as protein (71%), ribonucleic acid (15.8%), deoxyribonucleic acid (1.6%), and total carbohydrate (3.2%) did not vary significantly with the growth phase. Cells grown in the synthetic medium supplemented with yeast extract and tryptone had slightly higher protein content (76%), but the concentrations of the other cell constituents were similar and did not fluctuate much during growth. Nitrogen limiting growth resulted in somewhat lower ribonucleic acid content as well as slightly higher protein content than that in cells grown in nonlimiting medium. Methanospirillum hungatii did not accumulate any of the commonly known reserve materials under nitrogen or carbon and hydrogen limiting growth.


1972 ◽  
Vol 127 (2) ◽  
pp. 40P-40P ◽  
Author(s):  
M J Blackburn ◽  
T M Andrews ◽  
R W E Watts

1962 ◽  
Vol s3-103 (61) ◽  
pp. 25-35
Author(s):  
H. WALLACE

A larval lethal mutant of Xenopus laevis lacks true nucleoli but possesses analogous intranuclear organelles, here termed blobs, which are smaller and more numerous than nucleoli. Cytochemical tests reveal that blobs (like nucleoli) contain ribonucleic acid (RNA), arginine, and alkaline phosphatase, but probably no Feulgen-positive material. Anucleolate larvae are deficient in cytoplasmic RNA. By biochemical methods the nucleic acid content of anucleolate embryos is found to be normal at the tail-bud stage, but does not increase after this. By the time they hatch, anucleolate larvae are deficient in both RNA and deoxyribonucleic acid (DNA). The implications of this and related mutations on the formation and function of the nucleolus are considered. The term ‘blob’ is justified in that it would be misleading to regard such organelles as nucleoli produced by normally latent organizers.


Nature ◽  
1955 ◽  
Vol 176 (4472) ◽  
pp. 116-117 ◽  
Author(s):  
JEROME J. FREED ◽  
ELIZABETH UFFORD GREEN

1931 ◽  
Vol 8 (2) ◽  
pp. 133-150
Author(s):  
F. C. STOTT

1. The spring inshore migration of Echinus at Port Erin in 1930 started in early February and reached its maximum in the middle of March. Mature gonads were found at the end of February and throughout March and early April. At the end of June all gonads examined were spent. The sea temperature throughout this period was observed. It is probable that May was the chief month in which natural spawning took place. 2. A cycle of changes in the composition of the gonad are recorded from November 1929 to July 1930. The chief of these are: (a) In the males an increase in percentage dry weight as the gonads mature ; the opposite taking place in the females. (b) A large and simultaneous decrease in percentage glycogen in both male and female gonads prior to spawning followed by a great post-spawning increase. The fatty acid content does not alter with the maturation of the gonad, but an indication is given that an increase in the percentage of carbohydrates other than glycogen occurs. Hence it is suggested that glycogen is transformed in the maturing gonad into carbohydrate food reserves for the ripe eggs and sperm.


1994 ◽  
Vol 26 (2) ◽  
pp. 149-152 ◽  
Author(s):  
Kazuto Kunimi ◽  
Tadao Uchibayashi ◽  
Torn Hasegawa ◽  
Soo-Woong Lee ◽  
Mitsuo Ohkawa

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