Effect of freezing and thawing on survival of three bacterial isolates from an arctic soil

1978 ◽  
Vol 24 (12) ◽  
pp. 1468-1474 ◽  
Author(s):  
Louise M. Nelson ◽  
D. Parkinson
Keyword(s):  
Storage Time ◽  
Soil Extract ◽  
High Rate ◽  
Pseudomonas Sp ◽  
Meadow Soil ◽  
Bacterial Isolates ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Devon Island ◽  
Arctic Soil

Three isolates, a Pseudomonas sp., Bacillus sp., and Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to freezing and thawing at low rates under various conditions. When cells were frozen in sand or soil, survival was dependent on moisture level, storage time, and thaw rate. Pseudomonas M216 was most susceptible to freeze–thaw damage under these conditions. Arthrobacter M51 was the most resistant of the three isolates when frozen in sand or soil and when frozen at a high rate after growth at varing rates at 5 and 15 °C in carbon- or nitrogen-limited media. Pseudomonas M216 was more sensitive to freeze-thaw damage when NaCl was present in the freezing menstruum, even at low freezing rates. Survival of cells frozen in growth medium, water, saline, and soil extract was not affected by the freezing rate when it was less than 1 °C min−1. Soil extract did not protect cells from freeze–thaw damage any more than water and for Arthrobacter M51 survival was decreased when it was frozen in soil extract.

Effect of starvation on survival of three bacterial isolates from an arctic soil

1978 ◽  
Vol 24 (12) ◽  
pp. 1460-1467 ◽  
Author(s):  
Louise M. Nelson ◽  
D. Parkinson
Keyword(s):  
Survival Rates ◽  
Starvation Period ◽  
Bacterial Isolates ◽  
Starvation Stress ◽  
Gradual Decline ◽  
Carbon And Nitrogen ◽  
Devon Island ◽  
Endogenous Metabolism ◽  
Arctic Soil ◽  
Endogenous Substrates

Three isolates, a Pseudomonas sp., a Bacillus sp. and an Arthrobacter sp., which had been isolated from a meadow soil at Devon Island, Canada, were subjected to starvation under varying conditions. The viabilities of the three isolates during starvation for 30 days in a carbon-free medium was assessed after the organisms had been grown continuously at varying rates in carbon- and nitrogen-limited media at 5 and 15 °C. Pseudomonas M216 was the most resistant to starvation stress. Bacillus M153 the least, and Arthrobacter M51 was intermediate in its response. Cells grown and starved at 5 °C survived longer than those at 15 °C. Carbon-limited Bacillus and Arthrobacter cells grown at high rates prior to starvation survived longer than those grown slowly, while in nitrogen-limited Arthrobacter the reverse was observed. The pattern of endogenous metabolism of the three isolates during starvation at 15 °C for 10 days was similar to that observed in other organisms. Levels of endogenous substrates such as carbohydrate and protein showed a rapid decrease in the initial 20 h of starvation, followed by a gradual decline over the remainder of the starvation period. The rates of endogenous metabolism of the isolates were positively correlated with their survival rates during starvation.

Growth characteristics of three bacterial isolates from an arctic soil

1978 ◽  
Vol 24 (8) ◽  
pp. 909-914 ◽  
Author(s):  
Louise M. Nelson ◽  
D. Parkinson
Keyword(s):  
Low Temperatures ◽  
High Growth ◽  
Bacterial Isolates ◽  
Tundra Soils ◽  
Devon Island ◽  
Arctic Soil ◽  
Temperature Ranges ◽  
Effects Of Temperature ◽  
Taxonomic Characterization ◽  
Substrate Concentrations

Three bacterial isolates, a Pseudomonas sp., a Bacillus sp., and an Arthrobacter sp., commonly isolated from a hummocky sedge–moss meadow at Devon Island, N.W.T., Canada, were selected for further taxonomic characterization and for a study of the effects of temperature and limiting carbon source on growth. Pseudomonas M216 resembled P. putida and Bacillus M 153, B. carotarum. Arthrobacter M51 had growth-factor requirements which were more complex than those of any named species of that genus. The temperature ranges of growth indicated that Pseudomonas M216 and Arthrobacter M51 were psychrotrophic while Bacillus M153 was mesophilic. Growth in batch culture at limiting glucose concentrations enabled the calculation of Ks and Y values for each isolate. These were similar to those obtained for other organisms and Pseudomonas M216 and Bacillus M153 showed a high affinity for glucose. The nutritional versatility of Arthrobacter M51 and its ability to grow at low temperatures and the high growth rates and affinity of Pseudomonas M216 for low substrate concentrations may account for their competitive abilities in the natural environment, while the inability of Bacillus M153 to grow at low temperatures may limit its activity in tundra soils.

scholarly journals Strength and Microscopic Damage Mechanism of Yellow Sandstone with Holes under Freezing and Thawing

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
Huren Rong ◽  
Jingyu Gu ◽  
Miren Rong ◽  
Hong Liu ◽  
Jiayao Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Pore Size ◽  
Power Function ◽  
Damage Mechanism ◽  
Uniaxial Compression Test ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle ◽  
Microscopic Damage

In order to study the damage characteristics of the yellow sandstone containing pores under the freeze-thaw cycle, the uniaxial compression test of saturated water-stained yellow sandstones with different freeze-thaw cycles was carried out by rock servo press, the microstructure was qualitatively analyzed by Zeiss 508 stereo microscope, and the microdamage mechanism was quantitatively studied by using specific surface area and pore size analyzer. The mechanism of weakening mechanical properties of single-hole yellow sandstone was expounded from the perspective of microstructure. The results show the following. (1) The number of freeze-thaw cycles and single-pore diameter have significant effects on the strength and elastic modulus of the yellow sandstone; the more the freeze-thaw cycles and the larger the pore size, the lower the strength of the yellow sandstone. (2) The damage modes of the yellow sandstone containing pores under the freeze-thaw cycle are divided into five types, and the yellow sandstone with pores is divided into two areas: the periphery of the hole and the distance from the hole; as the number of freeze-thaw cycles increases, different regions show different microscopic damage patterns. (3) The damage degree of yellow sandstone is different with freeze-thaw cycle and pore size. Freeze-thaw not only affects the mechanical properties of yellow sandstone but also accelerates the damage process of pores. (4) The damage of the yellow sandstone by freeze-thaw is logarithmic function, and the damage of the yellow sandstone is a power function. The damage equation of the yellow sandstone with pores under the freezing and thawing is a log-power function nonlinear change law and presents a good correlation.

Review of Strength and Failure Criteria for Concrete After Freeze-Thaw Damage

2012 ◽  
Vol 253-255 ◽  
pp. 456-461
Author(s):  
Yan Fu Qin ◽  
Bin Tian ◽  
Gang Xu ◽  
Xiao Chun Lu
Keyword(s):  
Normal State ◽  
Concrete Strength ◽  
Failure Criteria ◽  
Concrete Durability ◽  
Freezing And Thawing ◽  
Normal Concrete ◽  
Strength Criteria ◽  
Freeze Thaw ◽  
Ordinary Concrete ◽  
Concrete Failure

Frost resistance research is one of the important subject of concrete durability, however strength criteria is an important part of the study of mechanical behavior of concrete. So far, about concrete failure criteria are almost for normal concrete, which the domestic and overseas scholars have comparative detailed research in every respect to it, and to freeze-thaw damage of concrete but few research. Based on the summary of the existing ordinary concrete strength and failure criteria in normal state and after freeze-thaw damage，this paper have a brief comment of failure criteria on concrete after freeze-thaw damage. For later research about concrete strength and failure criteria under freezing and thawing cycle provide the reference.

scholarly journals Supplementation of freezing and thawing media with brain-derived neurotrophic factor protects human sperm from freeze-thaw-induced damage

2016 ◽  
Vol 106 (7) ◽  
pp. 1658-1665.e4 ◽  
Author(s):  
Atefeh Najafi ◽  
Ebrahim Asadi ◽  
Adel R. Moawad ◽  
Saideh Mikaeili ◽  
Fardin Amidi ◽  
...  
Keyword(s):  
Neurotrophic Factor ◽  
Human Sperm ◽  
Brain Derived Neurotrophic Factor ◽  
Freezing And Thawing ◽  
Freeze Thaw

THE INFLUENCE OF SOIL AND ROOT EXTRACTS ON THE ASSOCIATIVE GROWTH OF SELECTED SOIL BACTERIA

1963 ◽  
Vol 9 (2) ◽  
pp. 187-197 ◽  
Author(s):  
E. C. S. Chan ◽  
H. Katznelson ◽  
J. W. Rouatt
Keyword(s):  
Mixed Culture ◽  
Soil Bacteria ◽  
Agar Medium ◽  
Soil Extract ◽  
Antagonistic Effect ◽  
Pseudomonas Sp ◽  
Population Changes ◽  
Agrobacterium Radiobacter ◽  
Root Extracts ◽  
Wheat Population

These studies are concerned with the growth interrelationships of mixed cultures of five soil organisms in soil extract and root extracts of 2-, 4-, and 8-week-old oats, soybeans, and wheat. Population changes of Agrobacterium radiobacter, Arthrobacter citreus, Azotobacter chroococcum, Bacillus cereus, and a Pseudomonas sp. in pure and mixed culture were followed by plating on selective media. B. cereus and A. chroococcum grew poorly alone or in mixed culture in the extracts. In soil extract, A. citreus predominated over, or was nearly equal in number to, the Gram-negative forms (Pseudomonas and Agrobacterium). In root extracts, Pseudomonas sp. always predominated over A. citreus in mixed culture. A. radiobacter was inhibited in mature root extracts (8-week-old plants) although in pure culture it recovered after a period. An antagonistic effect of Pseudomonas sp. on A. chroococcum plated on nitrogen-free agar medium was found to be related to the kind of agar used.

scholarly journals USING SMOS PASSIVE MICROWAVE DATA TO DEVELOP SMAP FREEZE/THAW ALGORITHMS ADAPTED FOR THE CANADIAN SUBARCTIC

2015 ◽  
Vol XL-1-W5 ◽  
pp. 365-368 ◽  
Author(s):  
P. Kalantari ◽  
M. Bernier ◽  
K. C. McDonal ◽  
J. Poulin
Keyword(s):  
Land Cover ◽  
Time Series Data ◽  
Passive Microwave ◽  
Soil Freezing ◽  
Series Data ◽  
Freezing And Thawing ◽  
Freeze Thaw ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle ◽  
The Impact

Seasonal terrestrial Freeze/Thaw cycle in Northern Quebec Tundra (Nunavik) was determined and evaluated with passive microwave observations. SMOS time series data were analyzed to examine seasonal variations of soil freezing, and to assess the impact of land cover on the Freeze/Thaw cycle. Furthermore, the soil freezing maps derived from SMOS observations were compared to field survey data in the region near Umiujaq. The objective is to develop algorithms to follow the seasonal cycle of freezing and thawing of the soil adapted to Canadian subarctic, a territory with a high complexity of land cover (vegetation, soil, and water bodies). Field data shows that soil freezing and thawing dates vary much spatially at the local scale in the Boreal Forest and the Tundra. The results showed a satisfactory pixel by pixel mapping for the daily soil state monitoring with a > 80% success rate with in situ data for the HH and VV polarizations, and for different land cover. The average accuracies are 80% and 84% for the soil freeze period, and soil thaw period respectively. The comparison is limited because of the small number of validation pixels.

scholarly journals Exploitation of endophytic Pseudomonas sp. for plant growth promotion and colonization in rice

2017 ◽  
Vol 9 (3) ◽  
pp. 1310-1316
Author(s):  
Gurjot Kaur ◽  
Poonam Sharma ◽  
Deepika Chhabra ◽  
Kailash Chand ◽  
Gurjit Singh Mangat
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Growth Promotion ◽  
Acc Deaminase ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Pgp Traits ◽  
P Solubilization ◽  
Fluorescent Pigment

The present investigation was carried out to exploit bacterial endophytes associated with root and leaf tissue of rice plant for plant growth promotion (PGP) and colonization study in vitro. Total 10 endophytic bacterial isolates (Pseudomonas sp.) were evaluate for PGP traits like P solubilization, production of Indole acetic acid (IAA), siderophore, ACC deaminase, protease, cellulase, fluorescent pigment, urease and denitrification activity. Out of 10 endophytic bacteria 30 %, 60 %, 20 %, 70 %, 10 % and 10 % were positive for siderophore, protease, cellulase, fluorescent pigment, urease and denitrification respectively. Maximum IAA production was recorded with isolate LRBLE7 (18.8 μgml-1) followed by LRBRE4 (16.0 μgml-1) and maximum P-solubilization was recorded with isolate LRBRE4 (5.8 mg 100 ml-1) followed by LRBLE7 (4.4 mg 100 ml-1). ACC deaminase production was recorded with isolate LRBLE6 (O.D=0.352 nm) followed by LRBRE5 (O.D=0.324nm). Three potential isolates (LRBRE4, LRBRE6 and LRBLE7) were selected on the basis of multiple PGP traits and were subjected to colonization study of rice seedling in vitro. Potential bacterial isolates can be exploited for improving growth and productivity in rice under sustainable management system.

Durability of concrete containing chloride-based accelerating admixtures

1990 ◽  
Vol 17 (1) ◽  
pp. 102-112
Author(s):  
T. Rezansoff ◽  
D. Stott
Keyword(s):  
Calcium Chloride ◽  
Dynamic Modulus ◽  
Linseed Oil ◽  
Standard Test ◽  
Test Method ◽  
Concrete Durability ◽  
Freezing And Thawing ◽  
Cement Ratio ◽  
Freeze Thaw ◽  
Water To Cement Ratio

The influence of CaCl2 or a chloride-based accelerating admixture on the freeze–thaw resistance of concrete was evaluated. Three air entrained mix designs were investigated using ASTM C666-84, Standard Test Method for Resistance of Concrete to Rapid Freezing and Thawing. All mix designs were similar, using cement contents of 340–357 kg/m3 of concrete, except for the addition of either 2% calcium chloride or 2% High Early Pozzolith, while no accelerating admixture was added to the control mix. The entire test program was repeated four times with water-to-cement ratio of 0.46 and three times with the ratio of 0.43. For the Pozzolith-accelerated concrete, half the samples were coated with boiled linseed oil in all seven series. For the control (unaccelerated) concrete, half the samples were coated with boiled linseed oil in one series for each water-to-cement ratio. Performance was monitored using the dynamic modulus of elasticity as obtained from transverse resonant frequency measurements. Weight loss of the specimens was also measured. Only the control samples (no accelerators) showed sufficient durability to satisfy the standard of maintaining at least 60% of the original dynamic modulus after 300 cycles of alternate freezing and thawing. Sealing with linseed oil showed inconsistent improvement in the durability in the various test series when defined in terms of the dynamic modulus; however, weight losses were the lowest of all categories and surface scaling was minimal. Key words: concrete, durability, freeze–thaw testing, calcium chloride, admixtures, sealants, air void system.

scholarly journals A PZT-Based Electromechanical Impedance Method for Monitoring the Soil Freeze–Thaw Process

Sensors ◽  
2019 ◽  
Vol 19 (5) ◽  
pp. 1107 ◽  
Author(s):  
Jicheng Zhang ◽  
Chuan Zhang ◽  
Jiahao Xiao ◽  
Jinwei Jiang
Keyword(s):  
Stress Wave ◽  
Lead Zirconate Titanate ◽  
Soil Freezing ◽  
Impedance Method ◽  
Active Sensing ◽  
Freezing And Thawing ◽  
Engineering Structures ◽  
Electromechanical Impedance ◽  
Freeze Thaw ◽  
Wave Signal

It is important to conduct research on the soil freeze–thaw process because concurrent adverse effects always occur during this process and can cause serious damage to engineering structures. In this paper, the variation of the impedance signature and the stress wave signal at different temperatures was monitored by using Lead Zirconate Titanate (PZT) transducers through the electromechanical impedance (EMI) method and the active sensing method. Three piezoceramic-based smart aggregates were used in this research. Among them, two smart aggregates were used for the active sensing method, through which one works as an actuator to emit the stress wave signal and the other one works as a sensor to receive the signal. In addition, another smart aggregate was employed for the EMI testing, in which it serves as both an actuator and a receiver to monitor the impedance signature. The trend of the impedance signature with variation of the temperature during the soil freeze–thaw process was obtained. Moreover, the relationship between the energy index of the stress wave signal and the soil temperature was established based on wavelet packet energy analysis. The results demonstrate that the piezoceramic-based electromechanical impedance method is reliable for monitoring the soil freezing and thawing process.

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