The lipids of four unusual non-pathogenic host-associated spirochetes

1975 ◽  
Vol 21 (11) ◽  
pp. 1877-1880 ◽  
Author(s):  
Brian P. Livermore ◽  
Russell C. Johnson
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
The Other ◽  
Long Chain Fatty Acids ◽  
Phosphatidyl Glycerol ◽  
Free Living ◽  
Complex Lipids ◽  
Monogalactosyl Diglyceride ◽  
Chain Lengths ◽  
Long Chain

The lipid compositions of two spirochetes isolated from the human oral cavity and two isolated from pig feces were examined. These isolates were unusual in that they did not require long-chain fatty acids for growth, as do the other host-associated spirochetes, but rather required isobutyric and valeric acids. Therefore, they could be cultured in a medium free of serum or fatty acid –albumin supplements. The major fatty acids synthesized were normal and iso fatty acids with 14 and 16 carbons. No unsaturated fatty acids were detected, nor were chain lengths longer than 16 carbons. The major complex lipids found were monogalactosyl diglyceride, phosphatidyl glycerol, and bis-phosphatidyl glycerol. Nitrogenous phospholipids, present in Treponema and Leptospira, were not synthesized by these novel strains. The data indicate an intermediate position of these isolates between Treponema and free-living Spirochaeta.

scholarly journals The Sinorhizobium meliloti LpxXL and AcpXL Proteins Play Important Roles in Bacteroid Development within Alfalfa

2009 ◽  
Vol 191 (14) ◽  
pp. 4681-4686 ◽  
Author(s):  
Andreas F. Haag ◽  
Silvia Wehmeier ◽  
Sebastian Beck ◽  
Victoria L. Marlow ◽  
Vivien Fletcher ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Sinorhizobium Meliloti ◽  
Lipid A ◽  
Long Chain Fatty Acids ◽  
Free Living ◽  
Long Chain ◽  
Chain Fatty Acids

ABSTRACT Free-living Sinorhizobium meliloti lpxXL and acpXL mutants lack lipid A very-long-chain fatty acids (VLCFAs) and have reduced competitiveness in alfalfa. We demonstrate that LpxXL and AcpXL play important but distinct roles in bacteroid development and that LpxXL is essential for the modification of S. meliloti bacteroid lipid A with VLCFAs.

scholarly journals The occurrence of polyenoic very long chain fatty acids with greater than 32 carbon atoms in molecular species of phosphatidylcholine in normal and peroxisome-deficient (Zellweger's syndrome) brain

1988 ◽  
Vol 253 (3) ◽  
pp. 645-650 ◽  
Author(s):  
A Poulos ◽  
P Sharp ◽  
D Johnson ◽  
C Easton
Keyword(s):  
Fatty Acids ◽  
Molecular Species ◽  
Carbon Chain ◽  
Normal Brain ◽  
Long Chain Fatty Acids ◽  
Brain Physiology ◽  
Chain Lengths ◽  
The Brain ◽  
Long Chain ◽  
Chain Fatty Acids

The n-6 tetra- and pentaenoic fatty acids with carbon chain lengths greater than 32 found in normal brain are located predominantly in a separable species of phosphatidylcholine. A similar phospholipid is found in increased amounts in the brain of peroxisome-deficient (Zellweger's syndrome) patients, but the fatty acid composition differs in that penta- and hexaenoic derivatives predominate. Our data strongly suggest that the polyenoic very long chain fatty acids are confined to the sn-1 position of the glycerol moiety, while the sn-2 position is enriched in saturated, monounsaturated and polyunsaturated fatty acids with less than 24 carbon atoms. It is postulated that these unusual molecular species of phosphatidylcholine may play some, as yet undefined, role in brain physiology.

The major fatty acids in whole milk fat and in a fraction obtained by crystallization from acetone

1969 ◽  
Vol 36 (2) ◽  
pp. 169-175 ◽  
Author(s):  
Sonja Mattsson ◽  
P. Swartling ◽  
R. Nilsson
Keyword(s):  
Fatty Acids ◽  
Milk Fat ◽  
Unsaturated Fatty Acids ◽  
Short Chain Fatty Acids ◽  
Insoluble Fraction ◽  
Long Chain Fatty Acids ◽  
Acetone Insoluble ◽  
Whole Milk ◽  
Long Chain ◽  
Chain Fatty Acids

SummarySummer and winter milk-fat samples from 14 dairies in Sweden were fractionated by crystallization from acetone solution (1:8) at 15 °C. The composition of the major fatty acids of the parent milk fat and of the acetone insoluble fraction were examined by GLC, and the gross triglyceride pattern by TLC on plates of silicic acid treated with silver nitrate.The fatty acid composition of the milk fat was similar to that of milk fat from other countries and varied according to season and also, to a smaller extent, from region to region. Four fractions, representing 33–45, 41–34, 18–14 and 7–6 % of the fat and which contained progressively smaller proportions of saturated acids, were obtained by TLC.The acetone insoluble glyceride (AIG) fraction was characterized by a smaller content of short-chain fatty acids and unsaturated fatty acids, and a larger content of saturated long-chain fatty acids, than the parent milk fat. AIGs from summer milk fat contained a larger proportion of C18 acids and a smaller proportion of C6–C16 acids than AIGs from winter milk fat.Four fractions representing 62–70, 15–8, 16–15 and 7 % of the AIG fraction were obtained by TLC. The distribution of the triglycerides in the AIG fraction differed from that in the parent milk fat, mostly in the relative amounts of glycerides in the 2 most saturated TLC fractions. The seasonal variation was largely confined to these 2 fractions.

scholarly journals THE EFFECT OF LIPIDS AND SERUM ALBUMIN ON BACTERIAL GROWTH

1947 ◽  
Vol 85 (1) ◽  
pp. 9-22 ◽  
Author(s):  
René J. Dubos
Keyword(s):  
Fatty Acids ◽  
Serum Albumin ◽  
Culture Medium ◽  
Unsaturated Fatty Acids ◽  
Sole Source ◽  
Water Soluble ◽  
Long Chain Fatty Acids ◽  
Microbial Species ◽  
Long Chain ◽  
Chain Fatty Acids

Long chain fatty acids have been found to exhibit both inhibitory and stimulatory effects on the growth of tubercle bacilli and of a certain unidentified micrococcus culture. The toxicity of the fatty acids was much reduced or abolished by (a) esterification, even when the resulting product was a water-soluble ester, and (b) addition of crystalline serum albumin to the culture medium; other proteins tested were inactive in this respect. Marked growth stimulation of the microorganisms studied was obtained when certain long chain fatty acids were added to the culture medium in the form of their water-soluble esters, or in admixture with adequate amounts of serum albumin. Abundant growth of the micrococcus resulted from the addition of oleic, linoleic, linolenic, or arachidonic acid (0.0001 to 0.001 per cent) to a mineral medium containing glucose as sole source of carbon; in the case of this microbial species, none of the other substances tested could substitute for these unsaturated fatty acids. Enhancement of growth of tubercle bacilli was obtained by adding to the medium 0.001 to 0.01 per cent of a variety of fatty acids (saturated or unsaturated) even in the absence of glucose or of any other readily available carbon compound. These results suggest that long chain fatty acids can affect the growth of different microbial species through different metabolic channels and that, in order to study the mechanism of these metabolic and growth reactions, it is essential to use the fatty acids under conditions where they cannot manifest their toxic properties.

scholarly journals Unsaturated Fatty Acids Control Biofilm Formation of Staphylococcus aureus and Other Gram-Positive Bacteria

Antibiotics ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 788
Author(s):  
Kamila Tomoko Yuyama ◽  
Manfred Rohde ◽  
Gabriella Molinari ◽  
Marc Stadler ◽  
Wolf-Rainer Abraham
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Linoleic Acid ◽  
Unsaturated Fatty Acids ◽  
Long Chain Fatty Acids ◽  
Gram Positive ◽  
Biofilm Inhibition ◽  
Gram Positive Bacteria ◽  
Long Chain ◽  
Chain Fatty Acids

Infections involving biofilms are difficult to treat due to increased resistances against antibiotics and the immune system. Hence, there is an urgent demand for novel drugs against biofilm infections. During our search for novel biofilm inhibitors from fungi, we isolated linoleic acid from the ascomycete Hypoxylon fragiforme which showed biofilm inhibition of several bacteria at sub-MIC concentrations. Many fatty acids possess antimicrobial activities, but their minimum inhibitory concentrations (MIC) are high and reports on biofilm interferences are scarce. We demonstrated that not only linoleic acid but several unsaturated long-chain fatty acids inhibited biofilms at sub-MIC concentrations. The antibiofilm activity exerted by long-chain fatty acids was mainly against Gram-positive bacteria, especially against Staphylococcus aureus. Micrographs of treated S. aureus biofilms revealed a reduction in the extracellular polymeric substances, pointing to a possible mode of action of fatty acids on S. aureus biofilms. The fatty acids had a strong species specificity. Poly-unsaturated fatty acids had higher activities than saturated ones, but no obvious rule could be found for the optimal length and desaturation for maximal activity. As free fatty acids are non-toxic and ubiquitous in food, they may offer a novel tool, especially in combination with antibiotics, for the control of biofilm infections.

Influencia de la clarificación por sedimentación en el contenido de ácidos grasos en mostos garnacha y Viura y su evolución durante la fermentación / Influence of clarification by sedimentation on the content of fatty acids in garnacha and Viura musts and their changes during the fermentation

1999 ◽  
Vol 5 (6) ◽  
pp. 471-478
Author(s):  
D. Torrea Goñi ◽  
A. García Sotro ◽  
C. Ancín Azpilicueta
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Control Sample ◽  
High Rate ◽  
Long Chain Fatty Acids ◽  
Sugar Consumption ◽  
Long Chain ◽  
Rate Of Consumption ◽  
Chain Fatty Acids

The influence of static sedimentation of garnacha and Viura musts on the concentration of fatty acids and their changes during fermentation is evaluated. A non-sedimented must was used as a control sample. The sedimentation of garnacha must reduced the concentration of saturated fatty acids (mainly stearic acid), although it did not totally eliminate any single one. During the first half of the fermen tation (up to 50% of sugar consumption) the medium-chain fatty acids excreted their highest concen tration in the sample being clarified. The long-chain fatty acids, both saturated and unsaturated, were consumed in both samples, although the saturated acids showed a higher rate of consumption in the contrul sample (57%) than in the sedimented sample (10%). The polyunsaturated fatty acids were consumed at a high rate in both samples. During the second half of the fermentation (from 50% sugar consumption until the end of the fermentation) the consumption of long-chain fatty acids, both saturated and unsaturated, was similar in the control sample and in the sedimented sample. The clarification of the Viura must reduced all the above unsaturated fatty acids, particularly linoleic (84%). In the first half of the fermentation, the consumption of long-chain fatty acids, both saturated and unsaturated, was greater in the control sample than in the sedimented sample; the same oc curred during the second half of the fermentation.

scholarly journals Adipocyte-specific Inactivation of Acyl-CoA Synthetase Fatty Acid Transport Protein 4 (Fatp4) in Mice Causes Adipose Hypertrophy and Alterations in Metabolism of Complex Lipids under High Fat Diet

2011 ◽  
Vol 286 (41) ◽  
pp. 35578-35587 ◽  
Author(s):  
Lena-Solveig Lenz ◽  
Jana Marx ◽  
Walee Chamulitrat ◽  
Iris Kaiser ◽  
Hermann-Josef Gröne ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
High Fat Diet ◽  
Fatty Acid Uptake ◽  
Acid Uptake ◽  
Long Chain Fatty Acids ◽  
High Fat ◽  
Complex Lipids ◽  
Long Chain ◽  
Chain Fatty Acids

Fatp4 exhibits acyl-CoA synthetase activity and is thereby able to catalyze the activation of fatty acids for further metabolism. However, its actual function in most tissues remains unresolved, and its role in cellular fatty acid uptake is still controversial. To characterize Fatp4 functions in adipocytes in vivo, we generated a mouse line with adipocyte-specific inactivation of the Fatp4 gene (Fatp4A−/−). Under standard conditions mutant mice showed no phenotypical aberrance. Uptake of radiolabeled palmitic and lignoceric acid into adipose tissue of Fatp4A−/− mice was unchanged. When exposed to a diet enriched in long chain fatty acids, Fatp4A−/− mice gained more body weight compared with control mice, although they were not consuming more food. Pronounced obesity was accompanied by a thicker layer of subcutaneous fat and greater adipocyte circumference, although expression of genes involved in de novo lipogenesis was not changed. However, the increase in total fat mass was contrasted by a significant decrease in various phospholipids, sphingomyelin, and cholesteryl esters in adipocytes. Livers of Fatp4-deficient animals under a high fat diet exhibited a higher degree of fatty degeneration. Nonetheless, no evidence for changes in insulin sensitivity and adipose inflammation was found. In summary, the results of this study confirm that Fatp4 is not crucial for fatty acid uptake into adipocytes. Instead, under the condition of a diet enriched in long chain fatty acids, adipocyte-specific Fatp4 deficiency results in adipose hypertrophy and profound alterations in the metabolism of complex lipids.

scholarly journals Mechanisms involved in the cytotoxic and cytoprotective actions of saturated versus monounsaturated long-chain fatty acids in pancreatic β-cells

2007 ◽  
Vol 194 (2) ◽  
pp. 283-291 ◽  
Author(s):  
Eleftheria Diakogiannaki ◽  
Shalinee Dhayal ◽  
Caroline E Childs ◽  
Philip C Calder ◽  
Hannah J Welters ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Formation Control ◽  
Unsaturated Fatty Acids ◽  
Lipid Synthesis ◽  
Monounsaturated Fatty Acids ◽  
Long Chain Fatty Acids ◽  
Β Cells ◽  
Β Cell ◽  
Long Chain

Long-chain saturated and monounsaturated fatty acids differ in their propensity to induce β-cell death in vitro with palmitate (C16:0) being cytotoxic, whereas palmitoleate (C16:1n-7) is cytoprotective. We now show that this cytoprotective capacity extends to a poorly metabolised C16:1n-7 derivative, methyl-palmitoleate (0.25 mM palmitate alone: 92 ± 4% death after 18 h; palmitate plus 0.25 mM methyl-palmitoleate: 12 ± 2%; P < 0.001). Palmitoleate and its methylated derivative also acted as mitogens in cultured β-cells (5-bromo-2-deoxyuridine incorporation – control: 0.15 ± 0.01 units; 0.25 mM palmitoleate: 0.22 ± 0.01 units; P < 0.05). It has been proposed that alterations in neutral lipid synthesis (particularly triacylglycerol (TAG) formation) might mediate the differential responses to saturated and unsaturated fatty acids and we have examined this proposition. Palmitate and palmitoleate both promoted β-cell phospholipid remodelling and increased TAG formation (control: 0.9 ± 0.1 nmol TAG/106 cells; 0.25 mM palmitate: 1.55 ± 0.07; 0.25 mM palmitoleate: 1.4 ± 0.05; palmitate plus palmitoleate: 2.3 ± 0.1). By contrast, methyl-palmitoleate failed to influence TAG levels (0.25 mM methyl-palmitoleate alone: 0.95 ± 0.06 nmol TAG/106 cells; methyl-palmitoleate plus palmitate: 1.5 ± 0.05) or its fatty acid composition in β-cells exposed to palmitate. The results suggest that monounsaturated fatty acids can promote cell viability and mitogenesis by a mechanism that does not require their metabolism and is independent of alterations in TAG formation.

scholarly journals The inhibitory effect of intestinal infusions of unsaturated long-chain fatty acids on forestomach motility of sheep

1983 ◽  
Vol 50 (1) ◽  
pp. 141-149 ◽  
Author(s):  
T. Nicholson ◽  
Shadia A. Omer
Keyword(s):  
Fatty Acids ◽  
Pancreatic Juice ◽  
Unsaturated Fatty Acids ◽  
Inhibitory Effect ◽  
Long Chain Fatty Acids ◽  
Inhibitory Potency ◽  
Significant Depression ◽  
Conscious Sheep ◽  
Long Chain

1. Emulsions of C18unsaturated fatty acids (FA) were infused for 2 h into the intestine of conscious sheep while motility of the reticulum and rumen was recorded.2. Statistically-significant depression of motility was found when 3 or 5 g FA/h were infused. At 1 g/h there were minor disruptions of motility.3. The inhibitory potency was not related to the extent of unsaturation of the FA.4. Receptors mediating the inhibitory effect were postulated to be distal to the iliac flexure of the duodenum, but the possible role of bile and pancreatic juice is discussed in relation to this problem.5. The probability that the effect was hormonally mediated is discussed.

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