Immunological investigations of Penicillium.: I. Serological reactivities of exocellular polysaccharides produced by six Penicillium species

1970 ◽  
Vol 16 (8) ◽  
pp. 687-694 ◽  
Author(s):  
J. F. Preston III ◽  
Erlinda Lapis ◽  
J. E. Gander
Keyword(s):  
Acid Hydrolysis ◽  
Acid Treatment ◽  
Cross Reactivity ◽  
Antigenic Specificity ◽  
Mild Acid Hydrolysis ◽  
Penicillium Species ◽  
Whole Cells ◽  
Exocellular Polysaccharides ◽  
Antigenic Reactivity ◽  
Mild Acid

The exocellular polysaccharides of Penicillium charlesii, P. chrysogenum, P. raistrickii, P. claviforme, and P. patulum contain galactofuranosyl residues since galactose is released by mild acid hydrolysis. Antisera from rabbits injected with whole cells of P. charlesii cross reacted with the exocellular polysaccharides from each species. The exocellular polymer from Penicillium various contained no galactose labile to mild acid treatment, and showed no cross reactivity with antisera to P. charlesii. No antigenic reactivity was observed in exocellular polysaccharides modified by mild acid hydrolysis. Oligosaccharides produced by mild acid hydrolysis which contained galactofuranosyl moieties as well as methyl α-D- and methyl β-D-galactofuranosides inhibited precipitate formation when the antigen was incubated with antisera. Treatment of the polysaccharides with 1 N NaOH did not alter its reactivity toward the antisera. It was concluded that several species of Penicillium elaborate exocellular polysaccharides containing galactofuranosyl residues and that these residues contribute to the antigenic specificity of the organism.

scholarly journals Presence of 3-O-methyl-l-xylose in the lipopolysaccharide of Pseudomonas maltophilia N.C.T.C. 10257

1977 ◽  
Vol 163 (1) ◽  
pp. 173-175 ◽  
Author(s):  
F Brown ◽  
D J Neal ◽  
S G Wilkinson
Keyword(s):  
Acid Hydrolysis ◽  
Mild Acid Hydrolysis ◽  
Whole Cells ◽  
Pseudomonas Maltophilia ◽  
Mild Acid

3-O-Methyl-L-xylose was isolated from whole cells of Pseudomonas maltophilia N.C.T.C. 10257. The sugar is a component of lipopolysaccharide from which a polysaccharide also containing L-rhamnose and L-xylose was released by mild acid hydrolysis. 3-O-Methyl-L-xylose was absent from five other strains of Ps. maltophilia and one strain of Pseudomonas geniculata.

scholarly journals Identification of N-acetyl-4–O-acetylneuraminyl-lactose in echidna milk

1974 ◽  
Vol 139 (2) ◽  
pp. 415-420 ◽  
Author(s):  
Michael Messer
Keyword(s):  
Sialic Acid ◽  
Acid Hydrolysis ◽  
Acid Treatment ◽  
Periodate Oxidation ◽  
Thiobarbituric Acid ◽  
Chromatographic Mobility ◽  
Mild Acid Hydrolysis ◽  
Acetylneuraminic Acid ◽  
Acid Reagent ◽  
Mild Acid

The identity of a novel form of sialyl-lactose found in milk of the echidna (Tachyglossus aculeatus) was investigated. The sialyl-lactose yielded equimolar amounts of N-acetylneuraminic acid and lactose during mild acid hydrolysis but was resistant to the action of a bacterial neuraminidase. A viral neuraminidase hydrolysed it to lactose plus a form of sialic acid that reacted positively with thiobarbituric acid reagent but whose chromatographic mobility was greater than that of N-acetylneuraminic acid. Treatment with alkali converted the sialyl-lactose into a substance with the same chromatographic mobility as N-acetylneuraminyl-(2→3)-lactose and made it susceptible to the action of bacterial neuraminidase. The sialyl-lactose contained one mol of ester (identified as acetyl), and released one mol of formaldehyde during periodate oxidation, per mol of sialic acid. It did not contain N-glycollylneuraminic acid. These results indicate that the sialyl-lactose is N-acetyl-4-O-acetylneuraminyl-(2→3)-lactose. Echidna milk contained, in addition, a small amount of N-acetylneuraminyl-(2→3)-lactose.

Amino acid sequence of cyanogen bromide fragment CB5 of human hemopexin

1989 ◽  
Vol 54 (3) ◽  
pp. 803-810 ◽  
Author(s):  
Ivan Kluh ◽  
Ladislav Morávek ◽  
Manfred Pavlík
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Acid Hydrolysis ◽  
Cyanogen Bromide ◽  
Polypeptide Chain ◽  
Amino Acid Residues ◽  
Mild Acid Hydrolysis ◽  
Methionine Residue ◽  
Cyanogen Bromide Fragment ◽  
Mild Acid

Cyanogen bromide fragment CB5 represents the region of the polypeptide chain of hemopexin between the fourth and fifth methionine residue (residues 232-352). It contains 120 amino acid residues in the following sequence: Arg-Cys-Ser-Pro-His-Leu-Val-Leu-Ser-Ala-Leu-Thr-Ser-Asp-Asn-His-Gly-Ala-Thr-Tyr-Ala-Phe-Ser-Gly-Thr-His-Tyr-Trp-Arg-Leu-Asp-Thr-Ser-Arg-Asp-Gly-Trp-His-Ser-Trp-Pro-Ile-Ala-His-Gln-Trp-Pro-Gln-Gly-Pro-Ser-Ala-Val-Asp-Ala-Ala-Phe-Ser-Trp-Glu-Glu-Lys-Leu-Tyr-Leu-Val-Gln-Gly-Thr-Gln-Val-Tyr-Val-Phe-Leu-Thr-Lys-Gly-Gly-Tyr-Thr-Leu-Val-Ser-Gly-Tyr-Pro-Lys-Arg-Leu-Glu-Lys-Glu-Val-Gly-Thr-Pro-His-Gly-Ile-Ile-Leu-Asp-Ser-Val-Asp-Ala-Ala-Phe-Ile-Cys-Pro-Gly-Ser-Ser-Arg-Leu-His-Ile-Met. The sequence was derived from the data on peptides prepared by cleavage of fragment CB5 by mild acid hydrolysis, by trypsin and chymotrypsin.

scholarly journals A mannosyl-carrier lipid of bovine adrenal meddulla and rat parotid

1975 ◽  
Vol 146 (3) ◽  
pp. 645-651 ◽  
Author(s):  
D A White ◽  
C J Waechter
Keyword(s):  
Acid Hydrolysis ◽  
Alkaline Hydrolysis ◽  
Deae Cellulose ◽  
Rapid Loss ◽  
Mild Acid Hydrolysis ◽  
Pig Liver ◽  
High Concentrations ◽  
Rat Parotid ◽  
Chloroform Methanol ◽  
Mild Acid

1. The transfer of mannose from GDP-(U-14-C)mannose into endogenous acceptors of bovine adrenal medullla and rat parotid was studied. The rapidly labelled product, a glycolipid, was partially purified and characterized. 2. It was stable to mild alkaline hydrolysis but yielded (14-C)mannose on mild acid hydrolysis. It co-chromatographed with mannosyl phosphoryl dolichol in four t.l.c. systems and on DEAE-cellulose acetate. Addition of dolichol phosphate or a dolichol phosphate-enriched fraction prepared from pig liver stimulated mannolipid synthesis. 3. The formation of mammolipid appeared reversible, since addition of GDP to a system synthesizing the mannolipid caused a rapid loss of label from the mannolipid. UDP-N-acetylglucosamine did not inhibit mannolipid synthesis except at high concentrations (2 mM), even though in the absence of GDP-mannose, N-acetylglucosamine was incorporated into a lipid having the properties of a glycosylated polyprenyl phosphate. 4. Mannose from GDP-mannose was also incorporated into two other acceptors, (2y being insoluble in chloroform-methanol (2:1, v/v) but soluble in choloroform-methanol-water (10:10:3, by vol.) and (ii) protein. These are formed much more slowly than the mannolipid. 5. Exogenous mannolipid served as a mannose donor for acceptors (i) and (ii), and it is suggested that transfer of mannose from GDP-mannose to mannosylated protein occurs via two intermediates, the mannolipid and acceptor (i).

Sequential Enzymatic and Mild-Acid Hydrolysis of By-Product of Carrageenan Process from Kappaphycus alvarezii

2019 ◽  
Vol 12 (2) ◽  
pp. 419-432 ◽  
Author(s):  
Fernando Roberto Paz-Cedeno ◽  
Eddyn Gabriel Solórzano-Chávez ◽  
Levi Ezequiel de Oliveira ◽  
Valéria Cress Gelli ◽  
Rubens Monti ◽  
...  
Keyword(s):  
Acid Hydrolysis ◽  
Kappaphycus Alvarezii ◽  
Mild Acid Hydrolysis ◽  
Hydrolysis Of ◽  
Mild Acid
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