Preservation of viable flexibacteria at low temperatures

1970 ◽  
Vol 16 (6) ◽  
pp. 441-444 ◽  
Author(s):  
Annika Sanfilippo ◽  
Ralph A. Lewin
Keyword(s):  
Liquid Nitrogen ◽  
Low Temperatures ◽  
Extreme Temperature ◽  
Dimethyl Sulphoxide ◽  
The Other

Most of the 28 species (91 strains) of flexibacteria tested without additives survived after freezing in liquid nitrogen (−196 °C): they included all six Microscilla spp., all three Cytophaga spp., both of the Herpetosiphon spp. tested, Flexithrix dorotheae, and seven (most strains) of the eight species of Flexibacter spp. All but 2 of the 19 strains of Saprospira grandis, but neither of the two other tested species of Saprospira, survived freezing to −196° without additives.Incorporation of 10% glycerol before freezing permitted survival of three of the sensitive strains of Flexibacter (F. aurantiacus copepodarum, F. flexilis iolanthae, and F. giganteus), but adversely affected two other species (F. elegans and F. ruber). Incorporation of 10% dimethyl sulphoxide (DMSO) had some similar effects. Freezing to −196° with glycerol conserved viable cultures of all 19 S. grandis strains, of S. toviformis, and of 2 of the 3 strains of S. thermalis.A less extreme temperature was less satisfactory. Stored at −22°, some or all strains of 6 species died out in less than 1 week; most strains of 12 others died after a few weeks; and 7 species survived for at least 21 weeks. (The other three were not tested.)

scholarly journals A NEW ABSOLUTE NOISE THERMOMETER AT LOW TEMPERATURES

1959 ◽  
Vol 37 (12) ◽  
pp. 1397-1406 ◽  
Author(s):  
H. J. Fink
Keyword(s):  
Liquid Nitrogen ◽  
Low Temperatures ◽  
Thermal Noise ◽  
Noise Source ◽  
The Other ◽  
Liquid Oxygen ◽  
Noise Thermometer ◽  
Boiling Points ◽  
Different Temperatures

If three resistors, which are kept at different temperatures, are arranged in form of a π network and if two of the thermal noise voltages appearing across the π network are multiplied together and averaged with respect to time, then under certain conditions the correlation between those voltages can be made zero. This condition is used to calculate the temperature of one noise source provided all the resistance values and the other temperatures are known. A noise thermometer of this kind was constructed which is capable of measuring temperatures below approximately 140° K. The boiling points of liquid oxygen and liquid nitrogen were determined absolutely within 0.2%. Between 1.3° K and 4.2° K the thermometer had to be calibrated due to errors arising in the equipment and the measured temperatures were then accurate within ± 1%.

scholarly journals Catalytic Conversion of n-C7 Asphaltenes and Resins II into Hydrogen Using CeO2-Based Nanocatalysts

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1301
Author(s):  
Oscar E. Medina ◽  
Jaime Gallego ◽  
Sócrates Acevedo ◽  
Masoud Riazi ◽  
Raúl Ocampo-Pérez ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Low Temperatures ◽  
Gaseous Mixture ◽  
H2 Production ◽  
The Other ◽  
Hydrogen Release ◽  
Catalytic Gasification ◽  
Three Samples ◽  
Main Decomposition ◽  
Catalytic Capacity

This study focuses on evaluating the volumetric hydrogen content in the gaseous mixture released from the steam catalytic gasification of n-C7 asphaltenes and resins II at low temperatures (<230 °C). For this purpose, four nanocatalysts were selected: CeO2, CeO2 functionalized with Ni-Pd, Fe-Pd, and Co-Pd. The catalytic capacity was measured by non-isothermal (from 100 to 600 °C) and isothermal (220 °C) thermogravimetric analyses. The samples show the main decomposition peak between 200 and 230 °C for bi-elemental nanocatalysts and 300 °C for the CeO2 support, leading to reductions up to 50% in comparison with the samples in the absence of nanoparticles. At 220 °C, the conversion of both fractions increases in the order CeO2 < Fe-Pd < Co-Pd < Ni-Pd. Hydrogen release was quantified for the isothermal tests. The hydrogen production agrees with each material’s catalytic activity for decomposing both fractions at the evaluated conditions. CeNi1Pd1 showed the highest performance among the other three samples and led to the highest hydrogen production in the effluent gas with values of ~44 vol%. When the samples were heated at higher temperatures (i.e., 230 °C), H2 production increased up to 55 vol% during catalyzed n-C7 asphaltene and resin conversion, indicating an increase of up to 70% in comparison with the non-catalyzed systems at the same temperature conditions.

Interaction Between Low Temperatures Spacers and Source Drain Extensions and Pockets for Both NMOS and PMOS of the 65 nm Node Technology

2006 ◽  
Vol 912 ◽  
Author(s):  
Nathalie Cagnat ◽  
Cyrille Laviron ◽  
Daniel Mathiot ◽  
Pierre Morin ◽  
Frédéric Salvetti ◽  
...  
Keyword(s):  
Oxide Layer ◽  
Hydrogen Content ◽  
Low Temperatures ◽  
Direct Contact ◽  
Fabrication Process ◽  
Experimental Results ◽  
The Other ◽  
Mos Transistors ◽  
Phosphorus Source ◽  
Activation Annealing

AbstractDuring the MOS transistors fabrication process, the source-drain extension areas are directly in contact with the oxide liner of the spacers stack. In previous works [1, 2, 3] it has been established that boron can diffuse from the source-drain extensions into the spacer oxide liner during the subsequent annealing steps, and that the amount of boron loss depends on the hydrogen content in the oxide, because it enhances B diffusivity in SiO2.In order to characterize and quantify the above phenomena, we performed test experiments on full sheet samples, which mimic either BF2 source-drain extensions over arsenic pockets implants, or BF2 pockets under arsenic or phosphorus source-drain extensions implants. Following the corresponding implants, the wafers were covered with different spacer stacks (oxide + nitride) deposited either by LPCVD, or PECVD. After appropriate activation annealing steps, SIMS measurements were used to characterize the profiles of the various dopants, and the corresponding dose loss was evaluated for each species.Our experimental results clearly evidence that LPCVD or PECVD spacer stacks have no influence on the arsenic profiles. On the other hand, phosphorus and boron profiles are affected. For boron profiles, each spacer type has a different influence. It is also shown that boron out-diffuses not only from the B doped source-drain extension in direct contact with the oxide layer, but also from the "buried" B pockets lying under n-doped source drain extension areas. All these results are discussed in term of the possible relevant mechanism.

scholarly journals The principal magnetic susceptibilities of neodymium sulphate octahydrate at low temperatures

1939 ◽  
Vol 170 (941) ◽  
pp. 266-271 ◽  
Keyword(s):  
Low Temperatures ◽  
Room Temperature ◽  
Energy Levels ◽  
Constant Factor ◽  
The Other ◽  
Crystalline Field ◽  
Cubic Symmetry ◽  
The Subject ◽  
The Difference ◽  
The One

The magnetic and other related properties of neodymium sulphate have been the subject of numerous investigations in recent years, but there is still a remarkable conflict of evidence on all the essential points. The two available determinations of the susceptibility of the powdered salt at low temperatures, those of Gorter and de Haas (1931) from 290 to 14° K and of Selwood (1933) from 343 to 83° K both fit the expression X ( T + 45) = constant over the range of temperature common to both, but the constants are not the same and the susceptibilities at room temperature differ by 11%. The fact that the two sets of results can be converted the one into the other by multiplying throughout by a constant factor suggested that the difference in the observed susceptibilities was due to some error of calibration. It could, however, also be due to the different purity of the samples examined though the explanation of the occurrence of the constant factor is then by no means obvious. From their analysis of the absorption spectrum of crystals of neodymium sulphate octahydrate Spedding and others (1937) conclude that the crystalline field around the Nd+++ ion is predominantly cubic in character since they find three energy levels at 0, 77 and 260 cm. -1 .* Calculations of the susceptibility from these levels reproduce Selwood’s value at room temperature but give no agreement with the observations-at other temperatures. On the other hand, Penney and Schlapp (1932) have shown that Gorter and de Haas’s results fit well on the curve calculated for a crystalline field of cubic symmetry and such a strength that the resultant three levels lie at 0, 238 and 834 cm. -1 , an overall spacing almost three times as great as Spedding’s.

Oxygen Stoichiometry and the High Tc Superconducting Oxides

MRS Bulletin ◽  
1989 ◽  
Vol 14 (1) ◽  
pp. 53-57 ◽  
Author(s):  
J-M. Tarascon ◽  
B.G. Bagley
Keyword(s):  
Optical Properties ◽  
Liquid Nitrogen ◽  
Broad Spectrum ◽  
Low Temperatures ◽  
Large Family ◽  
Oxygen Stoichiometry ◽  
High Tc ◽  
Metallic Conductivity ◽  
Superconducting Oxides ◽  
Technological Interest

Oxide compounds have been extensively studied through the years because they exhibit a broad spectrum of electrical, magnetic, and optical properties providing both scientific and technological interest. Most oxides are insulators, but a few of them (e.g., LiTi2O4 or BaPb1−x BixO3 show metallic conductivity and even superconductivity at low temperatures. The discovery of superconductivity at 35 K by Bednorz and Müller in the cuprate La-Ba-Cu-O system prompted the search for other high Tc compounds among this oxide family. Superconductivity above liquid nitrogen was then rapidly achieved with the Y-Ba-Cu-O system (Tc=90 K) and subsequently, with the Bi-Sr-Ca-Bu-O and Tl-Ba-Ca-Cu-O systems, Tc was raised to 110 K and then 125 K.A common feature of these new high Tc cuprates is that they belong to the large family of materials, termed perovskites, which have been studied over the years because of their ability to absorb or lose oxygen reversibly (i.e., for their nonstoichiometry in oxygen). It had been previously established in the field of superconductivity that Tc is extremely sensitive to compositional stoichiometry.

Effects of Dopants on the Nucleation and Growth of NiSi2 on Silicon

1987 ◽  
Vol 94 ◽  
Author(s):  
S. W. Lu ◽  
C. W. Nieh ◽  
J. J. Chu ◽  
L. J. Chen
Keyword(s):  
Electron Microscopy ◽  
Thin Films ◽  
Transmission Electron Microscopy ◽  
Low Temperatures ◽  
Nucleation And Growth ◽  
The Other ◽  
Size Factor ◽  
Atomic Size ◽  
Nickel Thin Films ◽  
Transmission Electron

ABSTRACTThe influences of implantation impurities, including BF2, B, F, As and P on the formation of epitaxial NiSi2 in nickel thin films on ion-implanted silicon have been investigated by transmission electron microscopy.The presence of BF2, B, and F atoms was observed to promote the epitaxial growth of NiSi2 at low temperatures. Little or no effect on the formation of NiSi2 was found in samples implanted with As or P ions.The results indicated that the influences of the implantation impurities are not likely to be electronic in origin. Good correlation, on the other hand, was found between the atomic size factor and resulting stress and NiSi2 epitaxy at low temperatures.

scholarly journals Systems with Size and Energy Polydispersity: From Glasses to Mosaic Crystals

Entropy ◽  
2020 ◽  
Vol 22 (5) ◽  
pp. 570
Author(s):  
Itay Azizi ◽  
Yitzhak Rabin
Keyword(s):  
Low Temperatures ◽  
The Other ◽  
Interaction Parameters ◽  
2D Systems ◽  
Small Particles ◽  
Strength Of Interaction ◽  
High Interaction ◽  
Mosaic Crystals ◽  
Dynamics Simulations ◽  
Entropic Effects

We use Langevin dynamics simulations to study dense 2d systems of particles with both size and energy polydispersity. We compare two types of bidisperse systems which differ in the correlation between particle size and interaction parameters: in one system big particles have high interaction parameters and small particles have low interaction parameters, while in the other system the situation is reversed. We study the different phases of the two systems and compare them to those of a system with size but not energy bidispersity. We show that, depending on the strength of interaction between big and small particles, cooling to low temperatures yields either homogeneous glasses or mosaic crystals. We find that systems with low mixing interaction, undergo partial freezing of one of the components at intermediate temperatures, and that while this phenomenon is energy-driven in both size and energy bidisperse systems, it is controlled by entropic effects in systems with size bidispersity only.

Ozonation of bromide-containing drinking waters:a delicate balance between disinfection and bromate formation

2000 ◽  
Vol 41 (7) ◽  
pp. 53-59 ◽  
Author(s):  
U. von Gunten ◽  
U. Pinkernell
Keyword(s):  
Temperature Dependence ◽  
Low Temperatures ◽  
The Other ◽  
Ozone Exposure ◽  
Oh Radicals ◽  
Strong Temperature Dependence ◽  
Control Options ◽  
The One ◽  
Kinetics Of ◽  
Ozonation Process

The occurrence of Cryptosporidium in raw waters and bromate formation during ozonation of bromide-containing waters leads to a difficult optimisation of ozonation processes. On the one hand inactivation of Cryptosporidium requires high ozone exposures, on the other hand under these conditions bromate formation is favored. In order to overcome this problem we need information about (i) the oxidant concentrations (ozone and OH radicals) during an ozonation process, (ii) kinetics of the inactivation of Cryptosporidium, (iii) kinetics and mechanism of bromate formation, and (iv) the reactor hydraulics. The strong temperature dependence of the inactivation of Cryptosporidium which results in a higher ozone exposure (time-integrated action of ozone) at low temperatures makes it more difficult to fulfil disinfection and bromate standards at low temperatures. Underthese conditions control options for bromate formation can be applied. Depressionof pH and addition of ammonia have been selected to be the best options. For a given ozone exposure both measures lead to a reduction of bromate formation in the order of 50%.

scholarly journals The specific heats of air, steam, and carbon dioxide

1923 ◽  
Vol 103 (720) ◽  
pp. 183-184 ◽  
Keyword(s):  
Carbon Dioxide ◽  
Title I ◽  
Low Temperatures ◽  
Close Agreement ◽  
The Other ◽  
British Association ◽  
Specific Heats

In a recent number is a criticism under the above title by Sir R. T. Glazebrook, of some figures given by me in a paper with the same title. I am accused of not giving Holborn and Henning's own figures, because I State that part of my curve is filled in from the researches of Swann, and of Holborn and Henning. As Swann and Holborn and Henning are not in agreement, it seems evident that one set figures cannot be used without some adjustment to the other set. These discrepancies were dealt with by the British Association Committee (of which Sir R. T. Glazebrook later became a member) in their 1908 Report, and the figures I give are in fairly close agreement with theirs for the low temperatures. I State in my paper (p. 492) that I find Holborn and Henning about 7½ percent. too low at 800°C. for air and steam, and I assume that the same error applies to the carbon dioxide. (Prof. Callendar suggests their error may be as much as 10 per cent. at 1400°C.) I have, therefore, distributed this error over the range for which I have used Holborn and Henning's figures. On this account it may be anticipated "that the figures at the higher temperatures are higher than the corresponding figures due to Holborn." I believe it is usual, when quoting the results of other workers, to attach their names to the figures quoted; this I have not done.

scholarly journals Interaction of solvents with membranal and soluble potassium ion-dependent enzymes

1970 ◽  
Vol 116 (1) ◽  
pp. 49-54 ◽  
Author(s):  
M. Mayer ◽  
Y. Avi-Dor
Keyword(s):  
Enzyme Activity ◽  
Pyruvate Kinase ◽  
Solvent Effects ◽  
Adenosine Triphosphatase ◽  
The State ◽  
Dimethyl Sulphoxide ◽  
The Other ◽  
Enzyme Structure ◽  
Wide Range ◽  
Muscle Pyruvate Kinase

The effects of dimethyl sulphoxide and glycerol on ox brain microsomal Na++K+-stimulated adenosine triphosphatase (EC 3.6.1.3), K+-stimulated p-nitrophenyl phosphatase and K+-dependent muscle pyruvate kinase (EC 2.7.1.40) were studied. Dimethyl sulphoxide at concentrations below 20% (v/v) was found to stimulate the p-nitrophenyl phosphatase and pyruvate kinase by increasing their affinity for K+ but to inhibit the Na++K+-stimulated adenosine triphosphatase. The latter enzyme activity was also inhibited by glycerol, which like dimethyl sulphoxide, stimulated the K+-activated p-nitrophenyl phosphatase at a wide range of concentrations. The solvent effects were promptly reversed by dilution. Similarity was found between glycerol and dimethyl sulphoxide, on one hand, and ATP, on the other, in their stimulatory effect and their ability to increase the ouabain- and oligomycin-sensitivity of the K+-stimulated p-nitrophenyl phosphatase. However, only the solvents, not the ATP, increased the binding of K+ by the microsomes. From the above findings it is suggested that solvents may act on K+-dependent enzymes by altering the state of solvation of the activating cation as well as by changing the enzyme structure.

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