LIPIDS OF CANDIDA UTILIS: CHANGES WITH GROWTH

1966 ◽  
Vol 12 (4) ◽  
pp. 775-785 ◽  
Author(s):  
P. S. S. Dawson ◽  
B. M. Craig
Keyword(s):  
Growth Rate ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Amino Acid ◽  
Candida Utilis ◽  
Gas Liquid Chromatography ◽  
Total Lipids ◽  
Quantitative Changes ◽  
Layer Chromatography ◽  
Qualitative Changes

Total lipids were extracted from cells of Candida utilis grown in batch, chemostat, and phased culture. Thin-layer chromatography of the extracts showed qualitative changes in the different lipid classes with growth. Gas–liquid chromatography was used to follow quantitative changes in the fatty acid constituents. Changes were found to be analogous to those previously reported for the amino acid pool, i.e., characteristic of the medium, of growth rate, and of the environment. The significance of the changes, and of the methods used to detect them, are discussed in relation to the growth and metabolism of the cell and of the culture.

Isolation and Identification of Egg Yolk Cholesteryl Esters

1972 ◽  
Vol 50 (12) ◽  
pp. 1414-1416 ◽  
Author(s):  
N. H. Tattrie
Keyword(s):  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Egg Yolk ◽  
Gas Liquid Chromatography ◽  
Cholesteryl Esters ◽  
Isolation And Identification ◽  
Layer Chromatography

Cholesteryl esters and cholesterol were isolated from egg yolk lipids by thin-layer chromatography. The cholesteryl esters and cholesterol contents of egg yolk lipids were 0.34% and 3.64%, respectively, as determined by gas–liquid chromatography. The fatty acid composition of the cholesteryl esters was 16:0, 24.7%; 16:1, 4.6%; 18:0, 6.8%; 18:1, 53.3%; and 18:2, 10.7%.

scholarly journals Identification of amino acid thiohydantoins directly by thin-layer chromatography and indirectly by gas-liquid chromatography after hydrolysis

1975 ◽  
Vol 147 (3) ◽  
pp. 435-438 ◽  
Author(s):  
M Rangarajan ◽  
A Darbre
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Indirect Method ◽  
Gas Liquid Chromatography ◽  
Two Dimensional ◽  
Layer Chromatography

A method is described for the identification of amino acid thiohydantoins by two-dimensional t.l.c. An indirect method for the determination of amino acid thiohydantoins is described which, after hydrolysis, the corresponding amino acids are determined by g.l.c.

Isolation of Cerebroside from Alfalfa Leaves

1973 ◽  
Vol 51 (6) ◽  
pp. 957-961 ◽  
Author(s):  
Seisuke Ito ◽  
Yasuhiko Fujino
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Solvent Extraction ◽  
Gas Liquid Chromatography ◽  
Long Chain Base ◽  
Silicic Acid Chromatography ◽  
Major Species ◽  
Layer Chromatography ◽  
Long Chain

Cerebroside was isolated from alfalfa (Medicago sativa) leaves by solvent extraction, mild alkaline hydrolysis, and silicic acid chromatography. The purified material was identified as cerebroside by thin-layer chromatography, infrared spectrum, and chemical analysis. Hydrolysates of the cerebroside were divided into fractions of fatty acid, sugar, and long-chain base, and analyzed for the respective composition mainly by gas–liquid chromatography. The major component fatty acids were hydroxyhexadecanoic, hydroxydocosanoic, and hydroxytetracosanoic acids. Dehydrophytosphingosine and sphingosine were the predominant constituent long-chain bases. The component sugar was only glucose. Based on the results, one of the major species of alfalfa cerebroside is suggested to be N-hydroxyhexadecanoyl-glucopyranosyl-dehydrophytosphingosine.

scholarly journals Chitinous fibrils in the lorica of the flagellate chrysophyte Poteriochromonas stipitata (syn. Ochromonas malhamensis).

1977 ◽  
Vol 73 (2) ◽  
pp. 311-321 ◽  
Author(s):  
W Herth ◽  
A Kuppel ◽  
E Schnepf
Keyword(s):  
Liquid Chromatography ◽  
Electron Microscope ◽  
Amino Acid ◽  
Thin Layer ◽  
Amino Acid Analysis ◽  
Gas Liquid Chromatography ◽  
X Ray Diffraction ◽  
X Ray ◽  
Diffraction Patterns ◽  
Layer Chromatography

Ordered microfibrils are formed on the membrane of the cytoplasmic tail of the alga Poteriochromonas after attachment to a substrate. The ultrastructure of native and extracted stalk fibrils was studied with electron microscope methods. In addition, the structural polysaccharide was characterized by hydrolyses, separation of the monomers by thin-layer chromatography, gas-liquid chromatography and amino acid analysis, and by X-ray diffraction. The alkali-resistant fibrils yielded mostly glucosamine upon extensive hydrolysis, and showed X-ray diffraction patterns similar to those of fugal chitin. It is concluded that the resistant core of the fibrils is chitinous.

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