Isolation and Identification of Egg Yolk Cholesteryl Esters

1972 ◽  
Vol 50 (12) ◽  
pp. 1414-1416 ◽  
Author(s):  
N. H. Tattrie
Keyword(s):  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Egg Yolk ◽  
Gas Liquid Chromatography ◽  
Cholesteryl Esters ◽  
Isolation And Identification ◽  
Layer Chromatography

Cholesteryl esters and cholesterol were isolated from egg yolk lipids by thin-layer chromatography. The cholesteryl esters and cholesterol contents of egg yolk lipids were 0.34% and 3.64%, respectively, as determined by gas–liquid chromatography. The fatty acid composition of the cholesteryl esters was 16:0, 24.7%; 16:1, 4.6%; 18:0, 6.8%; 18:1, 53.3%; and 18:2, 10.7%.

ANALYSIS OF CHLORORGANIC COMPOUNDS IN FODDER FOR BROILERCHICKENS BY THE METHOD OF GAS-LIQUID CHROMATOGRAPHY WITH MASSSPECTROMETER DETECTOR

2020 ◽  
Vol 1 (2) ◽  
pp. 205-208
Author(s):  
A.A. Parshutina ◽  
◽  
A.A. Solovyova ◽  
L.P. Satyukova ◽  
E.G. Shubina ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Organochlorine Pesticides ◽  
Mass Spectrometer ◽  
Broiler Chickens ◽  
Gas Liquid Chromatography ◽  
Layer Chromatography

The article shows the importance of the study of feed for broiler chickens on the content of organochlorine pesticides. These substances in significant concentrations can disrupt the development of birds and cause mass poisoning. The study of formula feed for broiler chickens «prestart» and «start» by two methodswas conducted: a certified method for the determination of organochlorine pesticides in feed and formula feed (thin layer chromatography) and a method for detecting pesticides not certified for feed (gas-liquid chromatography with mass spectrometer detector). During the experiment, the presence of organochlorine pesticides in several formulafeed samples was revealed.

Additional Cleanup of Samples on GLC Column for TLC Determination of Pesticide Residues

1967 ◽  
Vol 50 (3) ◽  
pp. 615-623
Author(s):  
Kenneth T Hartman
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Pesticide Residues ◽  
Gas Liquid Chromatography ◽  
Rf Values ◽  
Layer Chromatography

Abstract Gas-liquid chromatography (GLC) following conventional isolation procedures has been used to clean up pesticide residues for confirmation by thin layer chromatography (TLC). This procedure is more rapid and efficient than present cleanup procedures and permits the determination of pesticide residues that do not survive these rigorous acid or alkali treatments. The method also permits TLC confirmation of pesticide residues that have similar Rf values but different GLC retention times. Recoveries ranged from 85 to 105% for 25 of 28 pesticides tested

Thin Layer and Gas-Liquid Chromatography of Cholesterol in Fats and Oils. I. Development of Method

1969 ◽  
Vol 52 (4) ◽  
pp. 774-778
Author(s):  
C W Thorpe ◽  
Linda Pohland ◽  
D Firestone
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Unsaponifiable Matter ◽  
Fats And Oils ◽  
Cholesterol Content ◽  
Gas Liquid Chromatography ◽  
Animal Fat ◽  
Low Levels ◽  
Layer Chromatography

Abstract A inethod is described for analysis of sterols by thin layer chromatography-gas liquid chromatography (TLC-GLC). Sterols are isolated from other components of unsaponifiable matter by preparative TLC. The sterols are quantitatively removed from the TLC plate, extracted from the silica gel, and analyzed by GLC. This method has been used to detect low levels (2–3%) of animal fat in vegetable oil by measuring the cholesterol content of the animal fatvegetable oil admixtures.

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