Isolation of Cerebroside from Alfalfa Leaves

1973 ◽  
Vol 51 (6) ◽  
pp. 957-961 ◽  
Author(s):  
Seisuke Ito ◽  
Yasuhiko Fujino
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Solvent Extraction ◽  
Gas Liquid Chromatography ◽  
Long Chain Base ◽  
Silicic Acid Chromatography ◽  
Major Species ◽  
Layer Chromatography ◽  
Long Chain

Cerebroside was isolated from alfalfa (Medicago sativa) leaves by solvent extraction, mild alkaline hydrolysis, and silicic acid chromatography. The purified material was identified as cerebroside by thin-layer chromatography, infrared spectrum, and chemical analysis. Hydrolysates of the cerebroside were divided into fractions of fatty acid, sugar, and long-chain base, and analyzed for the respective composition mainly by gas–liquid chromatography. The major component fatty acids were hydroxyhexadecanoic, hydroxydocosanoic, and hydroxytetracosanoic acids. Dehydrophytosphingosine and sphingosine were the predominant constituent long-chain bases. The component sugar was only glucose. Based on the results, one of the major species of alfalfa cerebroside is suggested to be N-hydroxyhexadecanoyl-glucopyranosyl-dehydrophytosphingosine.

THE INFLUENCE OF DIETARY FAT ON THE FATTY ACID COMPOSITION OF LIVER, CARCASS, AND MILK OF RATS

1961 ◽  
Vol 39 (12) ◽  
pp. 1855-1863 ◽  
Author(s):  
Joyce L. Beare
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Dietary Fat ◽  
Rapeseed Oil ◽  
Corn Oil ◽  
Gas Liquid Chromatography ◽  
Long Chain

Fatty acids of liver, carcass, and milk of rats fed corn oil, rapeseed oil, partially hydrogenated herring oil, or margarine were examined by gas–liquid chromatography. Appreciable quantities of linoleic acid were maintained in the tissues and milk, even when the hydrogenated herring oil with a low level of linoleic acid was fed. The proportion of C20and C22acids deposited or secreted was related to that of the diet, and was highest with rapeseed oil. In the livers of rats fed each diet, long-chain, polyunsaturated acids were observed. The fatty acids of milk more closely reflected the dietary pattern than did those of the tissues.

Lipids and Component Fatty Acids of the Newfoundland Squid, Illex illecebrosus (Le Sueur)

1965 ◽  
Vol 22 (1) ◽  
pp. 131-137 ◽  
Author(s):  
Peter M. Jangaard ◽  
R. G. Ackman
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Acid Composition ◽  
Silicic Acid ◽  
Marine Species ◽  
Gas Liquid Chromatography ◽  
Silicic Acid Chromatography

The component fatty acids of the muscle, liver, and viscera lipids from the squid, Illex illecebrosus (Le Sueur), caught in Newfoundland waters have been determined by gas–liquid chromatography (GLC). Silicic acid chromatography was used to segregate the main groups of lipids and the fatty acid composition of each fraction was determined on two organosilicone polyester columns.The lipids from the muscle consisted mainly of phospholipids with three fatty acids, palmitic (16:0), eicosapentaenoic (20:5), and docosahexaneoic (22:6) acids making up 80% of the total. The liver contained mainly triglycerides with a fatty acid composition similar to liver oils in other marine species.

STEROL AND FATTY ACID COMPONENTS OF MYCELIUM OF FUSARIUM OXYSPORUM

1967 ◽  
Vol 13 (10) ◽  
pp. 1351-1355 ◽  
Author(s):  
A. N. Starratt ◽  
C. Madhosingh
Keyword(s):  
Fatty Acids ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Fusarium Oxysporum ◽  
Culture Medium ◽  
Chloroform Extract ◽  
Gas Liquid Chromatography ◽  
Ergosterol Peroxide ◽  
Oleic And Linoleic Acids

A chloroform extract of the mycelium of Fusarium oxysporum Schl. em. Sny. et Hans, was analyzed for sterols; three major components were identified as ergosterol, ergosterol peroxide, and cerevisterol. The ratio of ergosterol to ergosterol peroxide formed was variable. Palmitic, stearic, oleic, and linoleic acids were identified by gas–liquid chromatography as the principal fatty acids of this organism.Fusaric acid, a phytotoxin previously obtained by other investigators from a number of forms of F. oxysporum, was isolated from the culture medium.

scholarly journals Studies on Fatty Acid Composition and Proximate Analyses of Anethum Sowa L. (Dill) Seed

1970 ◽  
Vol 42 (4) ◽  
pp. 455-464 ◽  
Author(s):  
Md Moshfekus Saleh-E-In ◽  
Sudhangshu Kumar Roy
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Gas Liquid Chromatography ◽  
Good Source ◽  
Anethum Sowa ◽  
Proximate Analyses

Anethum sowa L. (Dill) seeds were investigated to determine the fatty acid composition and proximate analyses. The seeds contain 9.36 % fatty oil. The saturated and unsaturated fatty acids contributed 6.22% and 93.78% respectively of the oil. The per cent composition of the extracted oil was identified by Gas Liquid Chromatography (GLC). Among the six fatty acids identified from this study oleic acid contributed the highest proportion (87.10%), where as, linolenic, palmitic, stearic, behenic and arachidic all together contributed the rest (12.90%). Proximate analyses showed that A. sowa. seeds are good source of dietary fibre. Overall Dill seeds oil can be considered as a good source of oleic acid. Key words: Anethum sowa, dill seed oil, fatty acid composition, oleic acid, linolenic acid, Gas liquid chromatography. Bangladesh J. Sci. Ind. Res. 42(4), 455-464, 2007

FATTY ACID COMPOSITION OF NORMAL MINK TISSUES

1966 ◽  
Vol 44 (2) ◽  
pp. 179-185 ◽  
Author(s):  
B. L. Walker ◽  
V. F. Lishchenko
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Essential Fatty Acids ◽  
Methyl Esters ◽  
Dietary Factors ◽  
Gas Liquid Chromatography ◽  
Healthy Female ◽  
Kidney Liver ◽  
Layer Chromatography ◽  
Fatty Acid Compositions

Lipids, extracted from the adrenals, brain, erythrocytes, heart, kidney, liver, plasma, and spleen of normal healthy female mink, were transesterified with 1% sulphuric acid in methanol, and the resulting methyl esters were analyzed by gas–liquid chromatography after purification by thin-layer chromatography. All of the tissues examined contained higher concentrations of unsaturated than of saturated acids, the highest levels of unsaturated acids occurring in the lipids of heart, adrenals, and plasma, and of the essential fatty acids (ω6 series, with six carbon atoms after last double bond) in plasma, erythrocyte, and kidney lipids. The fatty acid compositions of mink tissues resemble those reported in the literature for the rat; detailed comparisons are not possible because of the known influence of dietary factors on tissue fatty acids.

Comparison of fatty acid composition of stable L-phase variants of Staphylococcus aureus induced by three different mechanisms

1977 ◽  
Vol 23 (10) ◽  
pp. 1478-1481 ◽  
Author(s):  
Joseph MacGee ◽  
Cheryl Glotzbecker ◽  
Chatrchai Watanakunakorn
Keyword(s):  
Fatty Acids ◽  
Staphylococcus Aureus ◽  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Acid Composition ◽  
Gas Liquid Chromatography ◽  
Fatty Acid Patterns ◽  
Phase Variants

The total saponifiable fatty acids of three stable L-phase variants of Staphylococcus aureus induced by cycloserine, methicillin, and lysostaphin were examined by gas-liquid chromatography. Five separate preparations of each of the three variants were examined. Twenty-nine fatty acids were identified. The fatty acid patterns of the three variants were very similar.

Fatty Acid Composition of Some Marine Animals from Various Depths

1967 ◽  
Vol 24 (5) ◽  
pp. 1101-1115 ◽  
Author(s):  
R. W. Lewis
Keyword(s):  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Acid Composition ◽  
Gas Liquid Chromatography ◽  
Marine Animals ◽  
Pelagic Species ◽  
Medium Chain ◽  
Long Chain

The fatty acid composition of 20 species of marine animals, primarily fishes and crustaceans, was determined by gas–liquid chromatography. The species came from various depths down to 4400 m. The results showed that the medium-chain saturated and the long-chain polyunsaturated acids decreased with increasing depth, while oleic acid increased. It was thought that this indicated the presence of large amounts of wax esters. A benthic, mud-feeding holothurian Scoloplanes theeli was shown to have a fatty acid composition markedly different from that of pelagic species. This was attributed in part to the bacterial components of its diet.

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