A SIMPLE PLAQUE ASSAY FOR MEASLES VIRUS IN MONOLAYERS OF THE FL CELL LINE

1963 ◽  
Vol 9 (3) ◽  
pp. 417-420 ◽  
Author(s):  
W. Yarosh ◽  
R. E. Armstrong
Keyword(s):  
Cell Line ◽  
Measles Virus ◽  
Plaque Assay

PLAQUE FORMATION BY POLIO AND MEASLES VIRUSES IN BS-C-1 (HOPPS) CELLS DERIVED FROM THE AFRICAN GREEN MONKEY

1965 ◽  
Vol 11 (3) ◽  
pp. 435-439 ◽  
Author(s):  
John Furesz ◽  
Pierre Moreau
Keyword(s):  
Cell Line ◽  
Measles Virus ◽  
Plaque Assay ◽  
Plaque Formation ◽  
African Green Monkey ◽  
High Passage ◽  
Green Monkey ◽  
Simple Medium ◽  
Low Passage ◽  
Passage Cells

BS-C-1 cells, cultivated in a simple medium designated M-E, were found to be suitable for a plaque assay of polio and measles viruses. In the plaque assay both low- and high-passage levels of the BS-C-1 cell line were highly susceptible to measles virus but titers of polioviruses were lower in the high-passage cells. Low-passage cells, preserved and stored at −196 °C for prolonged periods, are recommended for the titration of polio and measles viruses.

scholarly journals The effectiveness of antiviral agents with broad-spectrum activity against chikungunya virus varies between host cell lines

2018 ◽  
Vol 26 ◽  
pp. 204020661880758 ◽  
Author(s):  
Evelyn J Franco ◽  
Jaime L Rodriquez ◽  
Justin J Pomeroy ◽  
Kaley C Hanrahan ◽  
Ashley N Brown
Keyword(s):  
Antiviral Activity ◽  
Host Cell ◽  
Cell Line ◽  
Cell Lines ◽  
Broad Spectrum ◽  
Chikungunya Virus ◽  
Plaque Assay ◽  
A549 Cells ◽  
Western Hemisphere ◽  
Viral Burden

Chikungunya virus (CHIKV) is a mosquito-borne virus that has recently emerged in the Western Hemisphere. Approved antiviral therapies or vaccines for the treatment or prevention of CHIKV infections are not available. This study aims to evaluate the antiviral activity of commercially available broad-spectrum antivirals against CHIKV. Due to host cell-specific variability in uptake and intracellular processing of drug, we evaluated the antiviral effects of each agent in three cell lines. Antiviral activities of ribavirin (RBV), interferon-alfa (IFN-α) and favipiravir (FAV) were assessed in CHIKV-infected Vero, HUH-7, and A549 cells. CHIKV-infected cells were treated with increasing concentrations of each agent for three days and viral burden was quantified by plaque assay on Vero cells. Cytotoxic effects of RBV, FAV and IFN-α were also evaluated. Antiviral activity differed depending on the cell line used for evaluation. RBV had the greatest antiviral effect in HUH-7 cells (EC50 = 2.575 µg/mL); IFN-α was most effective in A549 cells (EC50 = 4.235 IU/mL); and FAV in HUH-7 cells (EC50 = 20.00 μg/mL). The results of our study show FAV and IFN-α are the most promising candidates, as their use led to substantial reductions in viral burden at clinically achievable concentrations in two human-derived cell lines. FAV is an especially attractive candidate for further investigation due to its oral bioavailability. These findings also highlight the importance of cell line selection for preclinical drug trials.

scholarly journals Evaluation of four cell lines for assay of infectious adenoviruses in water samples

2009 ◽  
Vol 7 (4) ◽  
pp. 650-656 ◽  
Author(s):  
Sunny C. Jiang ◽  
Jijun Han ◽  
Jian-Wen He ◽  
Weiping Chu
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Aquatic Environment ◽  
Plaque Assay ◽  
Environmental Water ◽  
Recreational Waters ◽  
293A Cell ◽  
Human Lung Carcinoma ◽  
Serotype 5 ◽  
Human Lung Carcinoma Cell

Human viral contamination in drinking and recreational waters poses health risks. The application of PCR-based molecular technology has advanced our knowledge of the occurrence and prevalence of human viruses in water; however, it has provided no information on viral viability and infectivity. Four human cell lines were compared for their sensitivity to different serotypes of human adenoviruses using the TCID50 test. The sensitivity of each cell line varied with different serotypes of adenovirus. Human embryonic kidney cell line 293A and human lung carcinoma cell line A549 were the most sensitive, especially to enteric adenovirus 40 and 41. Plaque assay of primary sewage samples showed 293A can detect viral plaques in 7 of 13 primary sewage samples tested. Adenoviruses were also isolated using 293A from environmental water concentrates. Cloning and sequencing of environmental adenoviral isolates indentified them to be aligned with adenoviruses serotype 40 and serotype 5. The result of this study suggests that plaque assay with 293A cell line is suitable for detection of adenovirus in the aquatic environment. Combining this cell culture with molecular methods for viral assay in the aquatic environment will provide critical information for risk assessment.

scholarly journals Defective translation of measles virus matrix protein in a subacute sclerosing panencephalitis cell line

Nature ◽  
1983 ◽  
Vol 305 (5930) ◽  
pp. 153-155 ◽  
Author(s):  
Michael J. Carter ◽  
Margaret M. Willcocks ◽  
Volker ter Meulen
Keyword(s):  
Cell Line ◽  
Measles Virus ◽  
Matrix Protein ◽  
Subacute Sclerosing Panencephalitis

scholarly journals Growth and plaque assay of parainfluenza viruses in an established canine kidney cell line

1980 ◽  
Vol 8 (4) ◽  
pp. 265-267
Author(s):  
Michael J. Browne ◽  
Gillian M. Sperrin ◽  
Malcolm R. Boyd
Keyword(s):  
Cell Line ◽  
Kidney Cell ◽  
Plaque Assay ◽  
Kidney Cell Line ◽  
Parainfluenza Viruses ◽  
Canine Kidney

Replication of measles virus in the human plasma cell leukemia-derived LICR-LON-HMy2 cell line

1989 ◽  
Vol 24 (3) ◽  
pp. 313-320
Author(s):  
Barry Ziola ◽  
Michael Morhart ◽  
Lynn Gilbert ◽  
Brenda Karvonen ◽  
Xiao Ping Chen
Keyword(s):  
Cell Line ◽  
Human Plasma ◽  
Plasma Cell ◽  
Measles Virus ◽  
Plasma Cell Leukemia ◽  
Cell Leukemia

scholarly journals Volume and surface changes in vero cell and its nucleus after infection with measles virus: a stereological study

2011 ◽  
Vol 2 (1) ◽  
pp. 18
Author(s):  
Ali Noorafshan ◽  
Mohammad Motamedifar ◽  
Saied Karbalay-Doust
Keyword(s):  
Vero Cell ◽  
Post Infection ◽  
Measles Virus ◽  
Plaque Assay ◽  
Vero Cells ◽  
Uninfected Control ◽  
Mean Cell Volume ◽  
Significant Difference ◽  
Infected Cells ◽  
The Mean

Measles virus has no or indistinctive cytopathic effects (CPE) in cell couture system. Employment of some detecting methods like plaque assay or stereologic experiments, as a method of detecting of viral infection in the cells would be applicable. The aim of this study was investigating the early changes in quantitative parameters of measles virus infected Vero cells. Stereological methods using invariator, were applied for the first time to estimate cell and nucleus volume and cell surface of the infected Vero cell line with the measles virus.This method can be applied on other cultured cells.Vero cells grown in tissue culture plates for 48 hours at 36˚C were infected with 100TCID50 of AiK strain of measles virus. Volume and surface of the infected Vero cells were studied at 4, 9 and 25 hours post infection along with uninfected control cells. The mean cell volume and surface of the cells infected with measles virus, increased ~87% and ~50%, respectively, 4 hours post-infection, as compared with the uninfected control. The nuclei did not show any differences. The mean parameters of infected cells in other time intervals showed no significant difference comparing with the control cells. Although there are other specific methods, stereology may be used as an integrated protocol to detect cytophatic changes of the measles virus infected cells early in the permissive cell culture system.

A sensitive plaque assay for bovine rotavirus in cultures of the bovine cell line GBK

1987 ◽  
Vol 13 (4) ◽  
pp. 383-387 ◽  
Author(s):  
Norio Hirano ◽  
Fumitoshi Sato ◽  
Katsuhiko Ono ◽  
Toshiaki Murakami ◽  
Minoru Matumoto
Keyword(s):  
Cell Line ◽  
Plaque Assay ◽  
Bovine Rotavirus ◽  
Bovine Cell
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