PROPAGATION OF RABIES VIRUS IN CULTURES OF HAMSTER KIDNEY CELLS

1960 ◽  
Vol 6 (5) ◽  
pp. 479-484 ◽  
Author(s):  
Paul Fenje
Keyword(s):  
Continuous Culture ◽  
Rabies Virus ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Culture Tube ◽  
High Infectivity

A strain of rabies fixed virus has been successfully cultivated in tissue cultures of hamster kidney cells. This confirms an earlier report by Kissling. In the experiments here recorded a special culture tube incorporating a dialyzing membrane made it possible to maintain the cells in continuous culture for many weeks. By using this technique it was possible to obtain culture fluids of high infectivity.

A RABIES VACCINE FROM HAMSTER KIDNEY TISSUE CULTURES: PREPARATION AND EVALUATION IN ANIMALS

1960 ◽  
Vol 6 (6) ◽  
pp. 605-609 ◽  
Author(s):  
Paul Fenje
Keyword(s):  
Salivary Glands ◽  
Rabies Virus ◽  
High Titer ◽  
Kidney Tissue ◽  
Rabies Vaccine ◽  
Tissue Cultures ◽  
Intramuscular Inoculation ◽  
Tissue Cells ◽  
Preparation And Evaluation ◽  
High Infectivity

A strain of fixed rabies virus adapted to hamster kidney tissue cells has produced culture fluids of high infectivity for rabbits and mice. These culture fluids were rendered non-infective by treatment with formaldehyde at a concentration of 8 m M. Rabbits immunized with this material produced autirabies antibody to a high titer and were subsequently proved to be resistant to intramuscular inoculation of rabies virus from the salivary glands of a naturally infected fox.

Some optimal conditions in freezing rabbit kidney cells for tissue cultures

Cryobiology ◽  
1968 ◽  
Vol 4 (4) ◽  
pp. 197-199 ◽  
Author(s):  
André Chagnon ◽  
Bertrand Couture ◽  
Vytautas Pavilanis
Keyword(s):  
Rabbit Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Optimal Conditions

scholarly journals THE ROLE OF METHIONINE DEFICIENCY IN POLIOVIRUS REPLICATION IN TISSUE CULTURES

1966 ◽  
Vol 123 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Soussan Mohajer ◽  
Janis Gabliks
Keyword(s):  
Amino Acids ◽  
Hela Cells ◽  
Inhibitory Effect ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Experimental Conditions ◽  
Methionine Deficiency ◽  
Intracellular Amino Acids

The role of methionine in poliovirus infection in HeLa and monkey kidney cells was investigated by using the methionine analogue l-ethionine. In the presence of 2.0 x 10–3 and 4.0 x 10–3 moles ethionine, the growth of HeLa and monkey kidney cells was significantly inhibited. Under the same experimental conditions, ethionine had no significant effect on the biosynthesis of two strains of poliovirus (Mahoney and Lansing) in HeLa cells, whereas in primary monkey kidney cells, it markedly inhibited the biosynthesis of the Lansing strain of poliovirus. HeLa cells partly depleted of their intracellular amino acids did not change the rate of viral biosynthesis. The inhibitory effect of ethionine on cell growth and viral biosynthesis was reversed by addition of an excess of l-methionine.

CHANGED RESPONSE OF EXCISED TOMATO ROOTS TO PYRIDOXIN DEFICIENCY FOLLOWING PROLONGED STERILE CULTURE

1962 ◽  
Vol 40 (8) ◽  
pp. 1107-1113 ◽  
Author(s):  
Claude Willemot ◽  
W. G. Boll
Keyword(s):  
Acetic Acid ◽  
Continuous Culture ◽  
Indole Acetic Acid ◽  
Vitamin B ◽  
Tissue Cultures ◽  
Free Medium ◽  
Sterile Culture ◽  
Tomato Roots ◽  
Cellular Control

A clone of excised tomato roots, selected originally for its marked requirement for vitamin B6, has been maintained in continuous culture in pyridoxin-containing medium since 1956. Growth in pyridoxin-free medium is now considerably greater than when the clone was originally isolated. A subclone of this clone was isolated in 1957 and maintained in medium in which pyridoxin was replaced with ethanolamine. This subclone does not show the change in response to pyridoxin deficiency. Because of this and other evidence it is concluded that the change occurs because of a change in the clone and is not an artefact due to faulty technique.The result is discussed in relation to (a) other reported changes in tissue cultures including the phenomenon of "adaptation" (accoutumance) to indole acetic acid, (b) the cellular control of metabolism.

NEW VIRAL AGENTS RECOVERED FROM TISSUE CULTURES OF MONKEY KIDNEY CELLS

1956 ◽  
Vol 63 (2) ◽  
pp. 204-215 ◽  
Author(s):  
ROBERT N. HULL ◽  
JAMES R. MINNER ◽  
JAMES W. SMITH
Keyword(s):  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures

Freezing and storing of monkey and rabbit kidney cells for tissue cultures

Cryobiology ◽  
1966 ◽  
Vol 3 (2) ◽  
pp. 81-84 ◽  
Author(s):  
André Chagnon ◽  
Vytautas Pavilanis
Keyword(s):  
Rabbit Kidney ◽  
Kidney Cells ◽  
Tissue Cultures

scholarly journals Interference Induced in GL-V3 Monkey Kidney Cells by Rabies Virus Strains

1981 ◽  
Vol 53 (2) ◽  
pp. 347-351 ◽  
Author(s):  
K. G. Nicholson ◽  
S. P. Bauer ◽  
P. Harrison
Keyword(s):  
Rabies Virus ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Virus Strains
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