CHANGED RESPONSE OF EXCISED TOMATO ROOTS TO PYRIDOXIN DEFICIENCY FOLLOWING PROLONGED STERILE CULTURE

1962 ◽  
Vol 40 (8) ◽  
pp. 1107-1113 ◽  
Author(s):  
Claude Willemot ◽  
W. G. Boll
Keyword(s):  
Acetic Acid ◽  
Continuous Culture ◽  
Indole Acetic Acid ◽  
Vitamin B ◽  
Tissue Cultures ◽  
Free Medium ◽  
Sterile Culture ◽  
Tomato Roots ◽  
Cellular Control

A clone of excised tomato roots, selected originally for its marked requirement for vitamin B6, has been maintained in continuous culture in pyridoxin-containing medium since 1956. Growth in pyridoxin-free medium is now considerably greater than when the clone was originally isolated. A subclone of this clone was isolated in 1957 and maintained in medium in which pyridoxin was replaced with ethanolamine. This subclone does not show the change in response to pyridoxin deficiency. Because of this and other evidence it is concluded that the change occurs because of a change in the clone and is not an artefact due to faulty technique.The result is discussed in relation to (a) other reported changes in tissue cultures including the phenomenon of "adaptation" (accoutumance) to indole acetic acid, (b) the cellular control of metabolism.

INCORPORATION OF SERINE-14C AND ETHANOLAMINE-14C INTO NITROGEN-CONTAINING PHOSPHATIDES AND EFFECTS OF MEDIUM CONTAINING ETHANOLAMINE ON PHOSPHATIDE BIOSYNTHESIS IN EXCISED TOMATO ROOTS

1967 ◽  
Vol 45 (10) ◽  
pp. 1863-1876 ◽  
Author(s):  
Claude Willemot ◽  
W. G. Boll
Keyword(s):  
Molecular Species ◽  
Phosphatidyl Choline ◽  
Phosphatidyl Ethanolamine ◽  
Phosphatidyl Serine ◽  
Vitamin B ◽  
Tomato Root ◽  
Sterile Culture ◽  
Working Hypothesis ◽  
Tomato Roots ◽  
Presence And Absence

Work was designed to study the decarboxylation of serine and biosynthesis of ethanolamine as part of an investigation into the replacement of vitamin B6by ethanolamine in the nutrition of excised tomato roots in sterile culture. A working hypothesis to explain the replacement involves repression, by ethanolamine, of a postulated vitamin B6dependent serine decarboxylase yielding ethanolamine.Evidence is presented, from studies using uniformly labelled serine-14C, serine-1-14C, serine-3-14C, and ethanolamine-1,2-14C (i) for the incorporation of serine and ethanolamine into the corresponding phosphatides, (ii) for the occurrence of a pathway whereby phosphatidyl serine is decarboxylated to phosphatidyl ethanolamine, which is further methylated to phosphatidyl choline, and (iii) for the existence of more than one molecular species of phosphatidyl ethanolamine.Experiments are reported in which uniformly labelled serine-14C was supplied in the presence and absence of the inhibitors ethionine, p-chloromercuribenzoate, or diethylethanolamine. The results suggest that in the excised tomato root a pathway of choline biosynthesis other than the phosphatide pathway may exist.Some evidence is given that the replacement of vitamin B6by ethanolamine decreases the decarboxylation of phosphatidyl serine.

Isolation and identification of IAA producing endosymbiotic bacteria from Gracillaria corticata (J. Agardh)

2016 ◽  
Vol 5 (12) ◽  
pp. 5179
Author(s):  
Ilahi Shaik* ◽  
P. Janakiram ◽  
Sujatha L. ◽  
Sushma Chandra
Keyword(s):  
Acetic Acid ◽  
Culture Filtrate ◽  
Indole Acetic Acid ◽  
Shoot Growth ◽  
High Salinity ◽  
Saline Soils ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Endosymbiotic Bacteria ◽  
Root And Shoot Growth

Indole acetic acid is a natural phytohormone which influence the root and shoot growth of the plants. Six (GM1-GM6) endosymbiotic bacteria are isolated from Gracilaria corticata and screened for the production of IAA out of six, three bacterial strains GM3, GM5 and GM6 produced significant amount of IAA 102.4 µg/ml 89.40 µg/ml 109.43 µg/ml respectively. Presence of IAA in culture filtrate of the above strains is further analyzed and confirmed by TLC. As these bacterial strains, able to tolerate the high salinity these can be effectively used as PGR to increase the crop yield in saline soils.

scholarly journals Characterization of Plant Growth-Promoting Traits and Inoculation Effects on Triticum durum of Actinomycetes Isolates under Salt Stress Conditions

Soil Systems ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 26
Author(s):  
Rihab Djebaili ◽  
Marika Pellegrini ◽  
Massimiliano Rossi ◽  
Cinzia Forni ◽  
Maria Smati ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Salt Stress ◽  
Plant Growth ◽  
Durum Wheat ◽  
Growth And Development ◽  
Indole Acetic Acid ◽  
Greenhouse Experiment ◽  
Hydrocyanic Acid ◽  
In Vitro Assays

This study aimed to characterize the halotolerant capability, in vitro, of selected actinomycetes strains and to evaluate their competence in promoting halo stress tolerance in durum wheat in a greenhouse experiment. Fourteen isolates were tested for phosphate solubilization, indole acetic acid, hydrocyanic acid, and ammonia production under different salt concentrations (i.e., 0, 0.25, 0.5, 0.75, 1, 1.25, and 1.5 M NaCl). The presence of 1-aminocyclopropane-1-carboxylate deaminase activity was also investigated. Salinity tolerance was evaluated in durum wheat through plant growth and development parameters: shoot and root length, dry and ash-free dry weight, and the total chlorophyll content, as well as proline accumulation. In vitro assays have shown that the strains can solubilize inorganic phosphate and produce indole acetic acid, hydrocyanic acid, and ammonia under different salt concentrations. Most of the strains (86%) had 1-aminocyclopropane-1-carboxylate deaminase activity, with significant amounts of α-ketobutyric acid. In the greenhouse experiment, inoculation with actinomycetes strains improved the morpho-biochemical parameters of durum wheat plants, which also recorded significantly higher content of chlorophylls and proline than those uninoculated, both under normal and stressed conditions. Our results suggest that inoculation of halotolerant actinomycetes can mitigate the negative effects of salt stress and allow normal growth and development of durum wheat plants.

Specificity of Phospholipid Binding to Indole Acetic Acid and Other Auxins

1974 ◽  
Vol 29 (1-2) ◽  
pp. 39-41 ◽  
Author(s):  
H. Veen
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
Phospholipid Binding ◽  
Primary Action

Abstract Studies in vitro on the binding of phospholipids with IAA, α-NAA and some of their biologically inactive analogues demonstrate that the observed interaction between IA A and lecithin is not related to the primary action of the hormone in plant growth.

Isolation, Culture and Plant Regeneration From Protoplasts of Nicotiana debneyi

1980 ◽  
Vol 7 (6) ◽  
pp. 635 ◽  
Author(s):  
WR Scowcroft ◽  
PJ Larkin
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Indole Acetic Acid ◽  
High Efficiency ◽  
Protoplast Culture ◽  
Callus Cultures ◽  
Dna Uptake ◽  
Mesophyll Protoplasts ◽  
Nicotiana Debneyi ◽  
Protoplast Division

Mesophyll protoplasts of two genetically distinct genotypes of N. debneyi were cultured with sustained division following a plating efficiency in excess of 50%. Fully fertile mature plants were regenerated from callus cultures derived from protoplasts. Shoots were induced in medium containing 1 mg/l 6-benzylaminopurine and 0.5 mg/I indole acetic acid. The repeatably high efficiency of protoplast culture was used to evaluate the quantitative effects of two drugs, kanamycin and trimethoprim, which effectively inhibited colony formation at concentrations of 100 and 50 �g/ml, respectively. An enhancer of DNA uptake, poly-L-ornithine, had virtually no effect on sustained protoplast division at a concentration of 7.5 �g/ml or less.

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