The influence of Mg and Ca ions on the maintenance of avian kidney cells in tissue cultures

1969 ◽  
Vol 85 (10) ◽  
pp. 292-293
Author(s):  
B. Sinkovic
Keyword(s):  
Kidney Cells ◽  
Tissue Cultures ◽  
Ca Ions ◽  
Avian Kidney

Some optimal conditions in freezing rabbit kidney cells for tissue cultures

Cryobiology ◽  
1968 ◽  
Vol 4 (4) ◽  
pp. 197-199 ◽  
Author(s):  
André Chagnon ◽  
Bertrand Couture ◽  
Vytautas Pavilanis
Keyword(s):  
Rabbit Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Optimal Conditions

scholarly journals THE ROLE OF METHIONINE DEFICIENCY IN POLIOVIRUS REPLICATION IN TISSUE CULTURES

1966 ◽  
Vol 123 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Soussan Mohajer ◽  
Janis Gabliks
Keyword(s):  
Amino Acids ◽  
Hela Cells ◽  
Inhibitory Effect ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Experimental Conditions ◽  
Methionine Deficiency ◽  
Intracellular Amino Acids

The role of methionine in poliovirus infection in HeLa and monkey kidney cells was investigated by using the methionine analogue l-ethionine. In the presence of 2.0 x 10–3 and 4.0 x 10–3 moles ethionine, the growth of HeLa and monkey kidney cells was significantly inhibited. Under the same experimental conditions, ethionine had no significant effect on the biosynthesis of two strains of poliovirus (Mahoney and Lansing) in HeLa cells, whereas in primary monkey kidney cells, it markedly inhibited the biosynthesis of the Lansing strain of poliovirus. HeLa cells partly depleted of their intracellular amino acids did not change the rate of viral biosynthesis. The inhibitory effect of ethionine on cell growth and viral biosynthesis was reversed by addition of an excess of l-methionine.

PROPAGATION OF RABIES VIRUS IN CULTURES OF HAMSTER KIDNEY CELLS

1960 ◽  
Vol 6 (5) ◽  
pp. 479-484 ◽  
Author(s):  
Paul Fenje
Keyword(s):  
Continuous Culture ◽  
Rabies Virus ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Culture Tube ◽  
High Infectivity

A strain of rabies fixed virus has been successfully cultivated in tissue cultures of hamster kidney cells. This confirms an earlier report by Kissling. In the experiments here recorded a special culture tube incorporating a dialyzing membrane made it possible to maintain the cells in continuous culture for many weeks. By using this technique it was possible to obtain culture fluids of high infectivity.

NEW VIRAL AGENTS RECOVERED FROM TISSUE CULTURES OF MONKEY KIDNEY CELLS

1956 ◽  
Vol 63 (2) ◽  
pp. 204-215 ◽  
Author(s):  
ROBERT N. HULL ◽  
JAMES R. MINNER ◽  
JAMES W. SMITH
Keyword(s):  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures

Freezing and storing of monkey and rabbit kidney cells for tissue cultures

Cryobiology ◽  
1966 ◽  
Vol 3 (2) ◽  
pp. 81-84 ◽  
Author(s):  
André Chagnon ◽  
Vytautas Pavilanis
Keyword(s):  
Rabbit Kidney ◽  
Kidney Cells ◽  
Tissue Cultures

NEW VIRAL AGENTS RECOVERED FROM TISSUE CULTURES OF MONKEY KIDNEY CELLS

1958 ◽  
Vol 68 (1) ◽  
pp. 31-44 ◽  
Author(s):  
ROBERT N. HULL ◽  
JAMES R. MINNER ◽  
CARMINE C. MASCOLI
Keyword(s):  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures

Morphogenesis of a Virus Isolated from a Human with Myelomonocytic Leukemia

1972 ◽  
Vol 30 ◽  
pp. 84-85
Author(s):  
Richard M. Jamison
Keyword(s):  
Electron Microscopy ◽  
Cell Line ◽  
Actinomycin D ◽  
Human Cell Line ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
Tissue Cultures ◽  
Peripheral Leukocytes ◽  
Myelomonocytic Leukemia

A virus has been isolated in tissue culture by co-cultivation in vitro of peripheral leukocytes from a leukemic donor with monkey kidney cells. This virus contains ribonucleic acid, its density is similar to that of members of the oncorna virus group, and its replication is inhibited by Actinomycin D; it is presumably a human oncornavirus. The virus has been detected by electron microscopy both in the peripheral leukocytes of the leukemic donor and in tissue cultures of the continuous monkey kidney cell line originally co-cultivated with these peripheral leukocytes. Plasma from the leukemic donor reacts in indirect immunofluorescence tests with the infected tissue cultures but not with non-infected controls. More recently, infection of a continuous human cell line (FL) has been detected by electron microscopy. Appropriate cell controls are negative.

scholarly journals A COMPARISON OF THE GROWTH OF SELECTED MYCOBACTERIA IN HELA, MONKEY KIDNEY, AND HUMAN AMNION CELLS IN TISSUE CULTURE

1958 ◽  
Vol 107 (2) ◽  
pp. 237-246 ◽  
Author(s):  
Charles C. Shepard
Keyword(s):  
Tissue Culture ◽  
Growth Rates ◽  
Monkey Kidney ◽  
Mycobacterium Fortuitum ◽  
Kidney Cells ◽  
Tubercle Bacillus ◽  
Tissue Cultures ◽  
Pathogenic Species ◽  
Subsequent Growth ◽  
Human Amnion

HeLa, monkey kidney, and human amnion cells in tissue cultures were compared as sites for the multiplication of strains of tubercle bacilli or original and reduced pathogenicity, and for several other species of mycobacteria capable of causing disease in humans. The arrangement of the pathogenic species inorder of their growth rates in HeLa cells was Mycobacterium fortuitum, Mycobacterium balnei, and the "yellow bacillus," followed closely by the tubercle bacillus. This order was also correct for these species in monkey kidney and human amnion cells, and is the same as that seen in bacteriological media. The arrangement of the strains of tubercle bacilli in order of their growth rates in all three types of cells was: H37Rv, then R1Rv, and lastly H37Ra, which multiplied about as slowly as BCG. An INH-resistant strain grew about as rapidly as H37Rv. Growth of the pathogenic species occurred at about the same rates in HeLa and monkey kidney cells, but was distinctly slower in human amnion cells, which are less active metabolically. Irradiation of the cells in doses up to 5000 r did not affect the subsequent growth of mycobacteria in them. Preliminary experiments with human leprosy bacilli indicate that they can be introduced into these cells in high numbers and that the bacilli then persist for the life of the cells.

In Vitro Regulation of 25-Hydroxyvitamin D3 Metabolism by Avian Kidney Cells in Serum-Free Medium

1979 ◽  
pp. 617-620
Author(s):  
R. Turner ◽  
G. A. Howard ◽  
J. I. Rader ◽  
B. L. Bottemiller ◽  
D. J. Baylink
Keyword(s):  
Kidney Cells ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Avian Kidney
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