SCREENING OF CARBOHYDRATES FOR SPORULATION OF BACILLI IN FLUID MEDIUM

1957 ◽  
Vol 3 (4) ◽  
pp. 639-642 ◽  
Author(s):  
S. K. Majumder ◽  
M. C. Padma
Keyword(s):  
Bacillus Cereus ◽  
Nutrient Broth ◽  
Shake Culture ◽  
Culture Experiment ◽  
Fluid Medium ◽  
Test Organisms ◽  
Active Multiplication ◽  
Fermentation Media

Carbohydrates that are used in fermentation media were screened for sporogenic activities on Bacillus cereus, B. subtilis, and B. megaterium. Out of the 15 carbohydrates tested, d-xylose (0.5%) in nutrient broth exerted maximum sporogenic stimulation on the test organisms. In shake culture experiment with B. subtilis the sporogenic action of xylose was evident even during the active multiplication of the organism in the medium.

A microbiological control of Melampsora medusae

1969 ◽  
Vol 47 (5) ◽  
pp. 711-715 ◽  
Author(s):  
Richard P. McBride
Keyword(s):  
Bacillus Cereus ◽  
Pseudotsuga Menziesii ◽  
Nutrient Broth ◽  
Effective Control ◽  
Bacillus Mycoides ◽  
Greenhouse Experiments ◽  
Aqueous Suspensions ◽  
Melampsora Medusae ◽  
Microbiological Control ◽  
Unidentified Species

Bacillus cereus Frankland and Frankland, Bacillus mycoides Flugge, and a third unidentified species of Bacillus occurred commonly as part of the saprophytic microflora on healthy foliage of Pseudotsuga menziesii (Mirb.) Franco. In greenhouse experiments, application of a mixture of nutrient broth cultures of these bacteria on P. menziesii seedlings resulted in control of the needle rust Melampsora medusae Thüm. Less effective control occurred when aqueous suspensions of the bacteria and cell-free (Millipore-filtered) filtrates of the nutrient broth cultures were applied to the seedlings, before inoculation.

scholarly journals Phytochemical and Antibacterial Properties of Ethanolic Seed Extracts of Chrysophyllum albidum (African Star Apple)

2016 ◽  
Vol 1 (1-2) ◽  
pp. 05-09 ◽  
Author(s):  
Samuel L. Oputah ◽  
Kolawole O. Ajanaku ◽  
Raphael C. Mordi ◽  
Joseph A. O. Olugbuyiro ◽  
Shade J. Olorunshola ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Test Organisms ◽  
Seed Extracts ◽  
African Star Apple

Phytochemical and antibacterial properties of ethanolic extract of the seeds of African Star Apple (Chrysophyllum albidum) were investigated. The phytochemical result revealed the presence of saponins, carbohydrates, flavonoids, quinones, cardiac glycosides, fatty acids and terpenoids. The antibacterial activity was studied using agar well diffusion method at different concentrations against six pathogenic bacterial strains, three Gram-positive (Staphylococcus aureus, Micrococcus varians and Bacillus cereus) and three Gram-negative (Escherichia coli, Pseudomonas aeruginosa and Proteus vulgaris). Significant inhibitory activities were exhibited by the ethanolic seed extracts for all test organisms except Bacillus cereus. Zone of inhibition of the crude ethanolic extract was correlated with that of a standard antibiotic Gentamicin, for antibacterial activity. The results indicated a notable inhibition of the bacterial growth.

Turbidimetric Evaluation of Bacterial Culture Resistance in Disinfectant Testing

1972 ◽  
Vol 55 (1) ◽  
pp. 219-223
Author(s):  
John H S Chen ◽  
L F Ortenzio
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacterial Growth ◽  
Bacterial Culture ◽  
Nutrient Broth ◽  
Turbidimetric Method ◽  
Salmonella Choleraesuis ◽  
Test Organisms ◽  
Test Cultures

Abstract A simple turbidimetric method has been developed for determining the degree of phenol resistance of cultures used for disinfectant testing. Net transmission values are used to assess t h e degree of resistance; these are based on t h e measurement of the total amount of light scattered by the bacterial growth i n the AOAC broth medium without interfering turbidity of the dissolved ingredients. A decrease in the net transmission value corresponds to a decrease in phenol resistance; the converse is also true . The transmission values obtained in a 10 month study of a total of 77 weekly r and om samples of AOAC nutrient broth media blanks showed no substantial differences among the broth media tested by the turbidimetric method. Net transmission values were obtained for 71 broth cultures of Salmonella choleraesuis, 74 of Staphylococcus aureus, and 78 of Pseudomonas aeruginosa having the same required resistance against phenol. These values, which fell within a wider range than would be expected, are given. The effect of the number of 10-carrier-soakings on the net transmission value and equivalent phenol resistance of the cultures was determined for 60 broth cultures of the 3 test organisms named above. Soaking wet cylinder carriers in a given volume of test cultures used for the AOAC use-dilution test directly reduced the net transmission value and equivalently decreased the phenol resistance of the cultures. The turbidimetric method has proven experimentally satisfactory i n several hundred determinations and in selecting test cultures of uniform quality for disinfectant testing.

Antibacterial activities and phytochemical properties of extracts of Dioscorea bulbifera Linn (Air Potatoe) tubers and peels against some pathogenic bacteria

2016 ◽  
Vol 5 (1) ◽  
pp. 20-26
Author(s):  
Omolade Mary Adeosun ◽  
◽  
Daniel Juwon Arotupin ◽  
Odeyemi Adebowale Toba ◽  
Alaba Adewole Adebayo ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Micrococcus Luteus ◽  
Antibacterial Properties ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Bioactive Constituents ◽  
Test Organisms ◽  
Health Promoting ◽  
Bioactive Ingredients

The antibacterial activities and bioactive constituents of ethanolic and aqueous extract of parts of the tuber against ten (10) clinical pathogens were determined, using agar well diffusion and standard techniques respectively. The proximate profile of this tuber included moisture content, ash, crude protein, fat, fibre and carbohydrate with varying percentages. The tuber was recorded to contain higher amount of saponin with the average of 24 mg/g, followed by cardiac glycosides with 13.13 mg/g, terpenoid with 8.48 mg/g, flavonoids followed with 5.36 mg/g and tannin with 4.21 mg/g was the least among the bioactive ingredients. Except for Proteus vulgaris, Serratia liquefaciens, Micrococcus luteus, Bacillus cereus and Citrobacter freundii, other test isolates were susceptible to the effect of the ethanolic extract of the peel of D. bulbifera at 500 µg/ml. High inhibition zones (between 17 and 22 mm) were recorded against 80% of the test organisms at 1000 µg/ml, except for 15 mm zone recorded against Bacillus cereus. The MIC and MBC of extract of D. bulbifera ranged in respect to the parts from 125 µg/ml to 500 µg/ml; and 250 µg/ml to 1000 µg/ml for peels and bulbils respectively. Antibacterial activity of the ethanolic and aqueous extracts of the bulbils of D. bulbifera was however, not profound in this present study compared to that of the peel. This study therefore, affirmed that D. bulbifera is a novel source of bioactive compounds which do not only enhance the antibacterial properties, but also ascertain its health promoting qualities.

Enzymatic reduction of mercurous and mercuric ions in Bacillus cereus

1981 ◽  
Vol 27 (2) ◽  
pp. 192-197 ◽  
Author(s):  
K. Izaki
Keyword(s):  
Escherichia Coli ◽  
Bacillus Cereus ◽  
Heavy Metal Ions ◽  
Heat Stability ◽  
Nutrient Broth ◽  
R Factor ◽  
Mercuric Ion ◽  
Enzymatic Reduction ◽  
Mercuric Ions ◽  
Sulfhydryl Compounds

A strain of Bacillus cereus, which can grow in nutrient broth containing 50 μM HgCl2, was isolated from soil. Mercurous or mercuric ion dependent oxidation of reduced NADPH was demonstrated in crude extracts of cells grown in nutrient broth containing 10 μM HgCl2. The properties of this mercuric reductase were similar to those of the enzymes from R factor bearing Escherichia coli in substrate specificity, heat stability, requirement of sulfhydryl compounds, sensitivity to some heavy metal ions, and molelcular weight.

scholarly journals Detection and Characterization of β-Lactam Resistance inBacillus cereusPTCC 1015

2004 ◽  
Vol 4 ◽  
pp. 622-627 ◽  
Author(s):  
Javad Behravan ◽  
Zeinab Rangsaaz
Keyword(s):  
Enzymatic Activity ◽  
Bacillus Cereus ◽  
Spectrophotometric Method ◽  
Nutrient Broth ◽  
Isolation And Characterization ◽  
Activity Measurements

In the present study, detection, isolation, and characterization of β-lactamases fromBacillus cereusPTCC 1015 were investigated.B. cereuswas inoculated in nutrient broth containing ampicillin (50 μg.ml−1) for 24 h (35°C, 200 rpm). Activity measurements were carried out against ampicillin (0.1 mg.ml−1) and cephalexin (0.08 mg.ml−1) by a spectrophotometric method at different conditions (pH 6–10, temperatures 25–45°C).Maximum penicillinase and cephalosporinase activity was observed at pH 7. The optimized temperatures for penicillinase and cephalosporinase activity were 30 and 40°C, respectively. At the above conditions, maximum enzymatic activity was calculated as 0.89 ± 0.014 and 0.037 ± 0.001 units against ampicillin and cephalexin.

The role of ER stress for the pathogenesis of SCA3 in primary cell culture experiment

2007 ◽  
Vol 34 (S 2) ◽  
Author(s):  
C Funke ◽  
J Hübener ◽  
H Wolburg ◽  
T Schmidt ◽  
H Toresson ◽  
...  
Keyword(s):  
Cell Culture ◽  
Er Stress ◽  
Primary Cell Culture ◽  
Primary Cell ◽  
Culture Experiment ◽  
Cell Culture Experiment
Sign in / Sign up

Export Citation Format

Share Document