scholarly journals Detection and Characterization of β-Lactam Resistance inBacillus cereusPTCC 1015

2004 ◽  
Vol 4 ◽  
pp. 622-627 ◽  
Author(s):  
Javad Behravan ◽  
Zeinab Rangsaaz
Keyword(s):  
Enzymatic Activity ◽  
Bacillus Cereus ◽  
Spectrophotometric Method ◽  
Nutrient Broth ◽  
Isolation And Characterization ◽  
Activity Measurements

In the present study, detection, isolation, and characterization of β-lactamases fromBacillus cereusPTCC 1015 were investigated.B. cereuswas inoculated in nutrient broth containing ampicillin (50 μg.ml−1) for 24 h (35°C, 200 rpm). Activity measurements were carried out against ampicillin (0.1 mg.ml−1) and cephalexin (0.08 mg.ml−1) by a spectrophotometric method at different conditions (pH 6–10, temperatures 25–45°C).Maximum penicillinase and cephalosporinase activity was observed at pH 7. The optimized temperatures for penicillinase and cephalosporinase activity were 30 and 40°C, respectively. At the above conditions, maximum enzymatic activity was calculated as 0.89 ± 0.014 and 0.037 ± 0.001 units against ampicillin and cephalexin.

scholarly journals Isolation and Characterization of Lactic Acid Bacteria in Philippine Fermented Milkfish Chanos chanos-Rice Mixture (Burong Bangus)

2018 ◽  
Vol 6 (2) ◽  
pp. 500-508
Author(s):  
Julie Ann A. Arcales ◽  
Garner Algo L.Alolod
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Enzymatic Activity ◽  
Antimicrobial Property ◽  
Food Products ◽  
The Philippines ◽  
Lactobacillus Delbrueckii ◽  
Bacterial Strains ◽  
Isolation And Characterization

Isolation and characterization of bacteria in food products are important to determine and distinguish the beneficial or harmful effects of microbiota in certain samples. Lactic acid bacteria in food products had long been associated to good factors as food preservatives and with added fermentation metabolites. This study isolated and characterized lactic acid bacteria from burong bangus. The culture and purification process of bacteria isolation resulted to 4 strains of lactic acid bacteria namely Enterococcus faecalis, Tetragenococcus muriaticus, Lactobacillus delbrueckii subp. delbrueckii and Carnobacterium divergens. High enzymatic activity were observed with E. faecalis particularly on lipase and protease assay. While C. divergens have no enzymatic activity against lipase, protease, amylase and cellulase. The antimicrobial property of L. delbrueckii is only susceptible to amoxicillin unlike the other three bacteria isolates. No antagonistic activity were observed with the four bacterial strains against Bacillus subtilis, Staphylococcus aureus and Escherichia coli. The result of this study showed promising benefits to the industry especially in developing countries like the Philippines because population are not yet so aware of this organisms and the benefits that can be derived through their consumption.

scholarly journals Inactivation of blasticidin S by Bacillus cereus. II. Isolation and characterization of a plasmid, pBSR8, from Bacillus cereus.

1988 ◽  
Vol 41 (2) ◽  
pp. 271-273 ◽  
Author(s):  
TOYOSHIGE ENDO ◽  
KAORI KOBAYASHI ◽  
NAOHIRO NAKAYAMA ◽  
TERUO TANAKA ◽  
TAKASHI KAMAKURA ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Isolation And Characterization ◽  
Blasticidin S

scholarly journals Isolation and Characterization of an RIP (Ribosome-Inactivating Protein)-Like Protein from Tobacco with Dual Enzymatic Activity

2003 ◽  
Vol 134 (1) ◽  
pp. 171-181 ◽  
Author(s):  
Neelam Sharma ◽  
Sang-Wook Park ◽  
Ramarao Vepachedu ◽  
Luigi Barbieri ◽  
Marialibera Ciani ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Ribosome Inactivating Protein ◽  
Isolation And Characterization

Enumeration, isolation and characterization of Bacillus cereus strains from Spanish raw rice

2002 ◽  
Vol 19 (6) ◽  
pp. 589-595 ◽  
Author(s):  
J.A. Sarrı́as ◽  
M. Valero ◽  
M.C. Salmerón
Keyword(s):  
Bacillus Cereus ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Bacillus cereus Phage vB_BceP-DLc1 Reveals the Largest Member of the Φ29-Like Phages

2020 ◽  
Vol 8 (11) ◽  
pp. 1750
Author(s):  
Chun Li ◽  
Xiaoming Yuan ◽  
Na Li ◽  
Juan Wang ◽  
Shubo Yu ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Dna Packaging ◽  
Model Organisms ◽  
Experimental Conditions ◽  
Isolation And Characterization ◽  
Bacillus Phage ◽  
Pharmaceutical Industries ◽  
High Concentrations ◽  
Ph Range

Bacillus phage φ29 and its relatives have been considered as one of the most important model organisms for DNA replication, transcription, morphogenesis, DNA packaging studies, and nanotechnology applications. Here, we isolated and characterized a new member of the φ29-like phage, named Bacillus cereus phage vB_BceP-DLc1. This phage, with a unique inserted gene cluster, has the largest genome among known φ29-like phages. DLc1 can use the surface carbohydrate structures of the host cell as receptors and only infects the most related B. cereus strains, showing high host-specificity. The adsorption rate constant and life cycle of DLc1 under experimental conditions were also determined. Not only stable under temperatures below 55 °C and pH range from 5 to 11, the new phage also showed tolerance to high concentrations of NaCl, 75% ethanol, chloroform, and mechanical vortex, which is preferable for practical use in the food and pharmaceutical industries.

Isolation and Characterization of Bacillus cereus Bacteriophages from Foods and Soil

2017 ◽  
Vol 9 (3) ◽  
pp. 260-269 ◽  
Author(s):  
Hyejin Oh ◽  
Dong Joo Seo ◽  
Su Been Jeon ◽  
Hyunkyung Park ◽  
Suntak Jeong ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Mannanase-producing Bacillus cereus Isolated from the Hindgut of Termites

2018 ◽  
Vol 17 (3) ◽  
pp. 116-123 ◽  
Author(s):  
Mairizal . ◽  
Yetti Marlida ◽  
Mirzah . ◽  
Fahmida Manin
Keyword(s):  
Bacillus Cereus ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of Exopolysaccharide producing Bacillus cereus from Brown Seaweed- Sargassum wightii

2019 ◽  
Vol 8 (09) ◽  
pp. 1302-1311
Author(s):  
V. A. Minimol ◽  
Pankaj Kishore ◽  
Ranjit K. Nadella ◽  
K. R. Sreelakshmi ◽  
S.S. Greeshma ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Brown Seaweed ◽  
Sargassum Wightii ◽  
Isolation And Characterization
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