EXPERIMENTAL INFECTION OF CHICK EMBRYOS WITH ENTAMOEBA INVADENS

1956 ◽  
Vol 2 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Eugene Meerovitch
Keyword(s):  
Experimental Infection ◽  
Chick Embryos ◽  
Fluid Medium ◽  
Entamoeba Invadens

Entamoeba invadens in chick embryos, incubated at 30 °C, invaded and produced changes in the liver. The embryos did not survive more than six days at 30 °C. after inoculation of the fluid medium, with or without E. invadens, and the inoculated amoebae did not survive the death of the embryo.

A PRELIMINARY NOTE ON THE EXPERIMENTAL INFECTION OF CHICK EMBRYOS WITH ENTAMOEBA INVADENS

1955 ◽  
Vol 1 (4) ◽  
pp. 284-287 ◽  
Author(s):  
Eugene Meerovitch
Keyword(s):  
Experimental Infection ◽  
Chick Embryos ◽  
Preliminary Note ◽  
Chicken Embryos ◽  
Entamoeba Invadens

Developing chicken embryos were infected intravenously with bacteria-free Entamoeba invadens. The parasites invaded the livers of the embryos, and caused the production of typical amoebic abscesses.

scholarly journals CULTURES OF ORGANIZED TISSUES

1922 ◽  
Vol 36 (4) ◽  
pp. 393-397 ◽  
Author(s):  
Albert Fischer
Keyword(s):  
Small Body ◽  
Chick Embryos ◽  
Malignant Cells ◽  
Intestinal Tissue ◽  
Fluid Medium ◽  
Long Period ◽  
Muscle Tissues ◽  
Pure Cultures ◽  
Cultivation In Vitro

An artificial organism, if one may so term it, composed of a complex of tissues, was cultivated for a long period of time. Small fragments of intestine from chick embryos 20 to 21 days old were placed in a suitable medium. The epithelium proliferated and completely covered the fragment of intestine after 4 to 6 days. A small body was thus formed, round or oblong in shape, surrounded by cylindrical epithelium and containing epithelial, connective, and muscle tissues, endothelium, and ameboid cells. After a month's cultivation in vitro, no necrosis had occurred. Therefore, it may be assumed that, through the intestinal epithelium, the medium supplied the intestinal tissue with sufficient nourishment. No uncontrolled proliferation took place after the epithelium bad surrounded the entire fragment. The cultivation of complex tissues will facilitate the study of the interactions of the different cells under various conditions. In some experiments, pure cultures of epithelial cells were grafted into such an "organism" without difficulty. The growth of malignant cells could be studied in the same way. When the "organism" was placed in a fluid medium, the epithelium remained normal but the stroma disappeared. It seems that plasma played an important rôle in the maintenance of the tissues in their normal condition.

GROWTH OF ENTAMOEBA INVADENS IN ORGANOTYPIC CULTURES OF EMBRYONIC CHICK INTESTINE

1961 ◽  
Vol 7 (5) ◽  
pp. 685-695 ◽  
Author(s):  
E. Meerovitch
Keyword(s):  
Metabolic Activity ◽  
Embryonic Chick ◽  
Mucous Secretion ◽  
Organotypic Cultures ◽  
Fluid Medium ◽  
Intact Tissue ◽  
Entamoeba Invadens

Entamoeba invadens from axenic and monoxenic cultures was inoculated into expiants of embryonic chick intestine, which were then cultured in perfusion chambers at 30 °C. The growth and metabolic activity of the explants in cultures were evaluated in terms of fibroblastic outgrowth and extent of liquefaction of the plasma clots in which they were embedded. The effects of several media used to fill the perfusion chambers on the survival of the explants were studied. It was found that amoebae developed best in those explants which themselves showed most vitality; this was in turn related to the kind of fluid medium used in the culture. Amoebae in the explants fed on mucous secretion and on dead cells and penetrated into intact tissue without apparent histolytic activity. It is suggested that the living explants provided the amoebae with certain enzymes which the latter were unable to produce at the temperature of incubation. Approximately 40% of all cultures made became positive for amoebae. This is attributed to the fact that not all explants retained the amoebae injected into them, before they were placed in culture.

scholarly journals A STUDY OF HUMAN SKIN GRAFTED UPON THE CHORIO-ALLANTOIS OF CHICK EMBRYOS

1938 ◽  
Vol 68 (6) ◽  
pp. 891-904 ◽  
Author(s):  
Ernest W. Goodpasture ◽  
Beverly Douglas ◽  
Katherine Anderson
Keyword(s):  
Blood Vessels ◽  
Human Skin ◽  
Experimental Infection ◽  
Collagen Fibers ◽  
Chick Embryos ◽  
Skin Grafts

Human skin grafted upon the chorio-allantoic membrane of chick embryos adheres and becomes nourished for as long as 10 days. Occasionally regrafts upon a second egg have succeeded and thus prolonged the vitality of the graft to 14 days. In successful experiments the epithelium of the chorio-allantois fuses with that of the graft, the collagen fibers of the corium interlace with those of the membrane after the separation or disappearance of the ectodermal layer, and the blood vessels of the chick anastomose, and unite by intervening pools of extravasated blood, with those of the graft. This vascular communication between the two tissues is largely responsible for the nourishment of the graft by affording a plasmatic circulation. Gradually there is a partial revascularization of the graft by an ingrowth of blood vessels from the chick membrane. Human skin grafts were susceptible to experimental infection by several viruses.

scholarly journals Isolation of Pigeonpox Virus from Severe Infection of Pigeons in Diyala province: Virological and Histopathological Study

2015 ◽  
Vol 39 (2) ◽  
pp. 72-78
Author(s):  
Karim S. Al-Ajeeli
Keyword(s):  
Experimental Infection ◽  
Chorioallantoic Membrane ◽  
Severe Infection ◽  
Immune Serum ◽  
Infected Tissue ◽  
Histopathological Study ◽  
Chick Embryos ◽  
Tissue Samples

     Pigeonpox virus was isolated from severe cases of avipoxvirus infection affecting 64 pigeons in Ba'aquba of Diyala governarate. The virus grew well on chorioallantoic membrane of chick embryos of 11-12 days old, and produced typical pock lesions. Histopathological sections of infected tissue samples revealed typical pox lesions. The virus was identified as Pigeonpox virus using of specific hyper immune serum and indirect immuno-flourescent and indirect immuno-peroxidase tests. The virus agglutinated RBCs of pigeon, fowl, turkey and duck. Experimental infection in pigeons produced moderate infection as compared to the diseased birds, while in chicken the virus produced mild infection.

Dexamethasone Effect on Embryonic Chick Respiratory Epithelium

1982 ◽  
Vol 40 ◽  
pp. 248-249
Author(s):  
M.R. Richter ◽  
R.V. Blystone
Keyword(s):  
Lung Development ◽  
Critical Role ◽  
Respiratory Epithelium ◽  
Chick Embryos ◽  
Embryonic Chick ◽  
White Leghorn ◽  
Lung Maturation ◽  
Synthetic Analogs ◽  
Lung Physiology ◽  
Avian Lung

Dexamethasone and other synthetic analogs of corticosteroids have been employed clinically as enhancers of lung development. The mechanism(s) by which this steroid induction of later lung maturation operates is not clear. This study reports the effect on lung epithelia of dexamethasone administered at different intervals during development. White Leghorn chick embryos were used so as to remove possible maternal and placental influences on the exogenously applied steroid. Avian lung architecture does vary from mammals; however, respiratory surfactant produced by the lung epithelia serves an equally critical role in avian lung physiology.

Labelling of non-A, non-B hepatitis infected chimpanzee hepatocytes with nuclease-gold conjugates

1984 ◽  
Vol 42 ◽  
pp. 250-251
Author(s):  
G. D. Gagne ◽  
M. F. Miller ◽  
D. A. Peterson
Keyword(s):  
Endoplasmic Reticulum ◽  
Experimental Infection ◽  
Uranyl Acetate ◽  
Type I ◽  
Cellular Injury ◽  
Type Ii ◽  
Tubular Structures ◽  
Type Iii ◽  
Type Iv ◽  
Infected Chimpanzee

Experimental infection of chimpanzees with non-A, non-B hepatitis (NANB) or with delta agent hepatitis results in the appearance of characteristic cytoplasmic alterations in the hepatocytes. These alterations include spongelike inclusions (Type I), attached convoluted membranes (Type II), tubular structures (Type III), and microtubular aggregates (Type IV) (Fig. 1). Type I, II and III structures are, by association, believed to be derived from endoplasmic reticulum and may be morphogenetically related. Type IV structures are generally observed free in the cytoplasm but sometimes in the vicinity of type III structures. It is not known whether these structures are somehow involved in the replication and/or assembly of the putative NANB virus or whether they are simply nonspecific responses to cellular injury. When treated with uranyl acetate, type I, II and III structures stain intensely as if they might contain nucleic acids. If these structures do correspond to intermediates in the replication of a virus, one might expect them to contain DNA or RNA and the present study was undertaken to explore this possibility.

Development of the Foramen Secundum in the Chick as Observed by Scanning Electron Microscopy

1976 ◽  
Vol 34 ◽  
pp. 212-213
Author(s):  
M.J.C. Hendrix ◽  
D.E. Morse
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Procaine Hydrochloride ◽  
Cardiac Anomaly ◽  
Chick Embryos ◽  
Congenital Cardiac Anomaly ◽  
Septal Defects ◽  
Critical Point Drying ◽  
Scanning Electron

Atrial septal defects are considered the most common congenital cardiac anomaly occurring in humans. In studying the normal sequential development of the atrial septum, chick embryos of the White Leghorn strain were prepared for scanning electron microscopy and the results were then extrapolated to the human heart. One-hundred-eighty chick embryos from 2 to 21 days of age were removed from their shells and immersed in cold cacodylate-buffered aldehyde fixative . Twenty-four embryos through the first week post-hatching were perfused in vivo using cold cacodylate-buffered aldehyde fixative with procaine hydrochloride. The hearts were immediately dissected free and remained in the fixative a minimum of 2 hours. In most cases, the lateral atrial walls were removed during this period. The tissues were then dehydrated using a series of ascending grades of ethanol; final dehydration of the tissues was achieved via the critical point drying method followed by sputter-coating with goldpalladium.

Immunocytochemical localization of desmin and vimentin in chick embryonic myocardial cells

1990 ◽  
Vol 48 (3) ◽  
pp. 448-449
Author(s):  
Yukiko Sugi
Keyword(s):  
Sodium Methoxide ◽  
Intermediate Filament Protein ◽  
Chick Embryos ◽  
Immunocytochemical Localization ◽  
Myocardial Cells ◽  
Immunofluorescence Study ◽  
Immunofluorescent Localization ◽  
Rabbit Igg ◽  
Semithin Sections ◽  
Filament Protein

In cultured skeletal muscle cells of chick, one intermediate filament protein, vimentin, is primarily formed and then synthesis of desmin follows. Coexistence of vimentin and desmin has been immunocytochemically confirmed in chick embryonic skeletal musclecells. Immunofluorescent localization of vimentin and desmin has been described in developing myocardial cells of hamster. However, initial localization of desmin and vimentin in early embryonic heart has not been reported in detail. By quick-freeze deep-etch method a loose network of intermediate filaments was revealed to exist surrounding myofibrils. In this report, immunocytochemical localization of desmin and vimentin is visualized in early stages of chick embryonic my ocardium.Chick embryos, Hamburger-Hamilton (H-H) stage 8 to hatch, and 1 day old postnatal chicks were used in this study. For immunofluorescence study, each embryo was fixed with 4% paraformaldehyde and embedded in Epon 812. De-epoxinized with sodium methoxide, semithin sections were stained with primary antibodies (rabbit anti-desmin antibody and anti-vimentin antibody)and secondary antibody (RITC conjugated goat-anti rabbit IgG).

Experimental infection of TCF αβdeficient mice with Citrobacterrodentium

2001 ◽  
Vol 120 (5) ◽  
pp. A690-A690
Author(s):  
J HART ◽  
E CHIN ◽  
C DANGLER ◽  
B SHEPPARD ◽  
D SCHAUER
Keyword(s):  
Experimental Infection
Sign in / Sign up

Export Citation Format

Share Document