A PRELIMINARY NOTE ON THE EXPERIMENTAL INFECTION OF CHICK EMBRYOS WITH ENTAMOEBA INVADENS

1955 ◽  
Vol 1 (4) ◽  
pp. 284-287 ◽  
Author(s):  
Eugene Meerovitch
Keyword(s):  
Experimental Infection ◽  
Chick Embryos ◽  
Preliminary Note ◽  
Chicken Embryos ◽  
Entamoeba Invadens

Developing chicken embryos were infected intravenously with bacteria-free Entamoeba invadens. The parasites invaded the livers of the embryos, and caused the production of typical amoebic abscesses.

EXPERIMENTAL INFECTION OF CHICK EMBRYOS WITH ENTAMOEBA INVADENS

1956 ◽  
Vol 2 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Eugene Meerovitch
Keyword(s):  
Experimental Infection ◽  
Chick Embryos ◽  
Fluid Medium ◽  
Entamoeba Invadens

Entamoeba invadens in chick embryos, incubated at 30 °C, invaded and produced changes in the liver. The embryos did not survive more than six days at 30 °C. after inoculation of the fluid medium, with or without E. invadens, and the inoculated amoebae did not survive the death of the embryo.

scholarly journals Susceptibility of Avian Species to Brucella Infection: A Hypothesis-Driven Study

Pathogens ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 77 ◽  
Author(s):  
Gamal Wareth ◽  
Ahmed Kheimar ◽  
Heinrich Neubauer ◽  
Falk Melzer
Keyword(s):  
Experimental Infection ◽  
Current Knowledge ◽  
Empirical Studies ◽  
Avian Species ◽  
Diagnostic Tools ◽  
Bacterial Disease ◽  
Chicken Embryos ◽  
Brucella Infection ◽  
Wide Range

Brucellosis is a highly contagious bacterial disease affecting a wide range of animals, as well as humans. The existence of the clinically diagnosed brucellosis in avian species is controversially discussed. In the current study, we set to summarize the current knowledge on the presence of brucellae in avian species. Anti-Brucella antibodies were monitored in different avian species using classical diagnostic tools. Experimental infection of chicken embryos induced the disease and resulted in the development of specific lesions. Few empirical studies have been performed in adult poultry. However, the isolation of brucellae from naturally-infected chickens has not been possible yet.

Experimental infection of chicken embryos with recently described Brucella microti: Pathogenicity and pathological findings

2015 ◽  
Vol 41 ◽  
pp. 28-34 ◽  
Author(s):  
Gamal Wareth ◽  
Denny Böttcher ◽  
Falk Melzer ◽  
Awad Ali Shehata ◽  
Uwe Roesler ◽  
...  
Keyword(s):  
Experimental Infection ◽  
Pathological Findings ◽  
Chicken Embryos

scholarly journals A STUDY OF HUMAN SKIN GRAFTED UPON THE CHORIO-ALLANTOIS OF CHICK EMBRYOS

1938 ◽  
Vol 68 (6) ◽  
pp. 891-904 ◽  
Author(s):  
Ernest W. Goodpasture ◽  
Beverly Douglas ◽  
Katherine Anderson
Keyword(s):  
Blood Vessels ◽  
Human Skin ◽  
Experimental Infection ◽  
Collagen Fibers ◽  
Chick Embryos ◽  
Skin Grafts

Human skin grafted upon the chorio-allantoic membrane of chick embryos adheres and becomes nourished for as long as 10 days. Occasionally regrafts upon a second egg have succeeded and thus prolonged the vitality of the graft to 14 days. In successful experiments the epithelium of the chorio-allantois fuses with that of the graft, the collagen fibers of the corium interlace with those of the membrane after the separation or disappearance of the ectodermal layer, and the blood vessels of the chick anastomose, and unite by intervening pools of extravasated blood, with those of the graft. This vascular communication between the two tissues is largely responsible for the nourishment of the graft by affording a plasmatic circulation. Gradually there is a partial revascularization of the graft by an ingrowth of blood vessels from the chick membrane. Human skin grafts were susceptible to experimental infection by several viruses.

scholarly journals Isolation and cultivation of bovine ephemeral fever virus in chickens and chicken embryos

1978 ◽  
Vol 81 (1) ◽  
pp. 1-7 ◽  
Author(s):  
M. A. Gaffar Elamin ◽  
P. B. Spradbrow
Keyword(s):  
Cell Culture ◽  
Vero Cell ◽  
Neutralizing Antibodies ◽  
Chick Embryos ◽  
Bovine Ephemeral Fever Virus ◽  
Immune Mouse ◽  
Chicken Embryos ◽  
Bovine Ephemeral Fever ◽  
Cattle Blood ◽  
Intravenous Inoculation

SUMMARYUnadapted bovine ephemeral fever (BEF) virus was isolated from cattle blood after intravenous inoculation into chicken embryos. Infected embryos died or hatched as abnormal chickens. The chick embryo was slightly less sensitive to unadapted BEF virus than were Vero cell cultures, but the use of embryos avoids the several blind passages that are required to isolate BEF virus in unweaned mice. Chick embryos were considerably less efficient than Vero cell culture or unweaned mice in detecting Vero cell-adapted and mouse-adapted BEF virus respectively.Viraemia was demonstrated in chicken embryos at 1–4 days and in one-day-old chickens at 1–3 days after intravenous inoculation of BEF virus. BEF virus was demonstrated by isolation and by immunofluoresence in heart, brain, lung and liver of chicken embryos at 1–5 days and in lung and liver of one-day-old chickens at 1–2 days, after intravenous inoculation. The isolated viruses were confirmed as BEF virus by neutralization with immune mouse ascitic fluid. BEF neutralizing antibodies were produced in 4-week-old and adult chickens after intravenous inoculation with BEF virus.

scholarly journals Isolation of Pigeonpox Virus from Severe Infection of Pigeons in Diyala province: Virological and Histopathological Study

2015 ◽  
Vol 39 (2) ◽  
pp. 72-78
Author(s):  
Karim S. Al-Ajeeli
Keyword(s):  
Experimental Infection ◽  
Chorioallantoic Membrane ◽  
Severe Infection ◽  
Immune Serum ◽  
Infected Tissue ◽  
Histopathological Study ◽  
Chick Embryos ◽  
Tissue Samples

     Pigeonpox virus was isolated from severe cases of avipoxvirus infection affecting 64 pigeons in Ba'aquba of Diyala governarate. The virus grew well on chorioallantoic membrane of chick embryos of 11-12 days old, and produced typical pock lesions. Histopathological sections of infected tissue samples revealed typical pox lesions. The virus was identified as Pigeonpox virus using of specific hyper immune serum and indirect immuno-flourescent and indirect immuno-peroxidase tests. The virus agglutinated RBCs of pigeon, fowl, turkey and duck. Experimental infection in pigeons produced moderate infection as compared to the diseased birds, while in chicken the virus produced mild infection.

150 THE DISTRIBUTION PATTERN OF PRIMORDIAL GERM CELLS IN EARLY CHICK EMBRYOS

2007 ◽  
Vol 19 (1) ◽  
pp. 192 ◽  
Author(s):  
Y. Nakamura ◽  
Y. Yamamoto ◽  
F. Usui ◽  
T. Ono ◽  
K. Takeda ◽  
...  
Keyword(s):  
Blood Vessels ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Central Zone ◽  
Staging System ◽  
Vascular Network ◽  
Chick Embryos ◽  
Chicken Embryos ◽  
The Future ◽  
The Mean

In all vertebrates, primordial germ cells (PGCs) appear during early stages of development in extragonadal sites, then they migrate to the gonad and give rise to ova or spermatozoa. Unlike in other species, however, in avian and reptile embryos, PGCs use the vascular system as a vehicle to transport them to the future gonadal region where they leave the blood vessels. The present study was carried out to know the details of this unique migration pathway and the proliferation of endogenous PGCs in chicken embryos. Whole of the chicken embryos during stages X [Roman numerals refer to the staging system of Eyal-Giladi and Kochav (1976 Dev. Biol. 49, 321–327) to 17 (Arabic numerals refer to the staging system of Hamburger and Hamilton (1951 J. Morphol. 88, 49-82))] or embryonic blood during stages 12 to 17 were immunohistochemical stained using specific antibody raised against chicken vasa homolog (CVH), which could be recognized as a marker for chicken PGCs. The distribution patterns and populations of PGCs in embryos were observed under a stereomicroscope. The numbers of PGCs were presented mean and standard deviation (mean � SD). Anti-CVH staining revealed the distribution and population of chicken PGCs in early chick embryos. PGCs existed mainly in the area pellucida and concentrated in the central zone at stage X. The mean number of PGCs per embryo at this stage was 130.4 � 31.9. With the formation of primitive streak, PGCs were carried anteriorly to the edge of the blastoderm. The PGCs scattered anteriorly began to concentrate to the anterior point of the head on the dorsal side of stage 10 embryos. The average number of PGCs per embryo at stage 10 was 439.3 � 93.6. The mean numbers of PGCs per embryo during stages X to 10 increased gradually as development progressed to stage 10. We found the entrance point of PGCs from anterior edge of the blastoderm to the vascular network during stages 10 to 11. In the blood, PGCs could be detected from all of the samples during stages 12 to 17. In contrast, no PGC was recognized in the future gonadal region before stage 14, and then they began to appear in the same region at stage 15. The mean numbers of PGCs that located in the future gonadal region during stages 15 to 17 increased intensively and were 97.3 � 57.3, 200.3 � 113.5, and 327.6 � 102.4, respectively. Interestingly, the numbers of PGCs within future gonadal region during stages 15 to 17 were consistently and significantly different (P < 0.05) between the left and right side of the region. The results suggest that chicken PGCs move from extraembryonic area to the vascular network during stages 10 to 11, circulate in the blood stream, and finally, they begin to leave the blood vessels actively and migrate to the future gonadal region at stage 15.

Dexamethasone Effect on Embryonic Chick Respiratory Epithelium

1982 ◽  
Vol 40 ◽  
pp. 248-249
Author(s):  
M.R. Richter ◽  
R.V. Blystone
Keyword(s):  
Lung Development ◽  
Critical Role ◽  
Respiratory Epithelium ◽  
Chick Embryos ◽  
Embryonic Chick ◽  
White Leghorn ◽  
Lung Maturation ◽  
Synthetic Analogs ◽  
Lung Physiology ◽  
Avian Lung

Dexamethasone and other synthetic analogs of corticosteroids have been employed clinically as enhancers of lung development. The mechanism(s) by which this steroid induction of later lung maturation operates is not clear. This study reports the effect on lung epithelia of dexamethasone administered at different intervals during development. White Leghorn chick embryos were used so as to remove possible maternal and placental influences on the exogenously applied steroid. Avian lung architecture does vary from mammals; however, respiratory surfactant produced by the lung epithelia serves an equally critical role in avian lung physiology.

Sign in / Sign up

Export Citation Format

Share Document