EXPERIENCES WITH A NEW ANTIGEN FOR A COMPLEMENT FIXATION TEST IN TUBERCULOSIS

1955 ◽  
Vol 1 (9) ◽  
pp. 794-798
Author(s):  
N. A. Labzoffsky
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Clinical Improvement ◽  
Ultrasonic Vibration ◽  
Antibody Titer ◽  
Guinea Pigs ◽  
Clinical Cure ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
New Method

A new method for the preparation of complement fixing antigen from Mycobacterium tuberculosis is outlined. The procedure consists of treatment of tubercle bacilli with pyridine, washing the sediment, resuspending it in saline, and finally exposing it to ultrasonic vibration. The antigen so obtained is nonviable, not anticomplementary, and appears to be specific. The results so far obtained with sera from infected guinea pigs and from human tuberculous patients indicate that complement fixing antibodies appear regularly in both. There is a suggestion that in human patients fall in antibody titer is associated with clinical improvement and disappearance with clinical cure.

scholarly journals A COMPLEMENT FIXATION TEST FOR POLIOMYELITIS

1955 ◽  
Vol 102 (2) ◽  
pp. 133-150 ◽  
Author(s):  
Nathalie J. Schmidt ◽  
Edwin H. Lennette ◽  
Keyword(s):  
Tissue Culture ◽  
Antibody Response ◽  
Clinical Diagnosis ◽  
Antibody Titer ◽  
Virus Type ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Laboratory Diagnosis ◽  
Fixation Test ◽  
Dual Infections

A macroscopic (tube) complement fixation test for poliomyelitis, using infected tissue culture fluids, is described. The test was applied to 27 individuals with a clinical diagnosis of poliomyelitis. In 18 patients it was possible to make a laboratory diagnosis of poliomyelitis on the basis of a rise in complement-fixing antibody titer and in 4 others on the basis of a high stationary antibody titer. One individual gave a high and equal antibody response to two virus types, 3 others had no detectable antibody, and 1 appeared not to have poliomyelitis. Heterotypic reactions were encountered, but gave little difficulty in interpreting homologous responses. In those patients from whom a virus had been recovered, the serologic findings corresponded to the virus type recovered. The possible occurrence of dual infections with the viruses of poliomyelitis and Western equine and St. Louis encephalitis is discussed.

NEW COMPLEMENT FIXING ANTIGEN FOR SERODIAGNOSIS OF GONORRHOEA

1961 ◽  
Vol 7 (5) ◽  
pp. 715-723
Author(s):  
N. A. Labzoffsky ◽  
A. E. Kelen
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Laboratory Diagnosis ◽  
Fixation Test ◽  
New Method ◽  
Strain Selection ◽  
The Past

A new method of preparing gonococcal antigen for use in the complement fixation test is presented. Briefly, the procedure consists of extracting Neisseria gonorrhoeae cells with pyridine and then exposing the washed sediment to ultrasonic treatment.The new method is superior to that of Price by yielding highly potent, genus-specific and stable antigens, which are free of anticomplementary properties and possess a broader antigenic valence and a longer range of working antigenic power. It is simpler and more practical than Torrey's modification of the Price method since the lengthy and laborious procedure of strain selection is eliminated.In routine use for the past several years, the new antigen has proved to be specific and reliable in the complement fixation test. The test itself provides a useful supplementary aid to the laboratory diagnosis of gonorrhoea.

A STUDY OF VARIOUS FRACTIONS OF BRUCELLA ABORTUS: II. COMPLEMENT FIXATION AND INTRADERMAL TESTS WITH ALCOHOLIC PRECIPITATE

1935 ◽  
Vol 12 (1) ◽  
pp. 125-132
Author(s):  
Ronald Gwatkin
Keyword(s):  
Skin Test ◽  
Brucella Abortus ◽  
Guinea Pigs ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Skin Reactions ◽  
Three Samples ◽  
Intradermal Tests

Alcoholic precipitates of Brucella abortus showed high antigenic qualities in the complement fixation test. Dialysis and filtration did not lower this activity. The precipitates gave rise to reactions in infected guinea pigs, in dilutions which produced no change in normal animals. Filtration did not modify the results. In two out of three samples dialysis did not lower the activity of the suspensions as skin test antigens. The addition of formol did not modify the skin reactions.

AGAR DIFFUSION PRECIPITATION AND COMPLEMENT FIXATION TESTS APPLIED TO THE STUDY OF THE ANTIGENIC RELATIONSHIPS BETWEEN CHROMOGENIC ACID-FAST BACTERIA AND MYCOBACTERIUM TUBERCULOSIS

1958 ◽  
Vol 4 (6) ◽  
pp. 565-570 ◽  
Author(s):  
Edith Mankiewicz
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Precipitation Reaction ◽  
Acid Fast Bacilli ◽  
Agar Diffusion ◽  
Antigenic Relationships ◽  
Diffusion Precipitation

The place of two serological techniques, the agar diffusion precipitation reaction and the complement fixation test in the classification of chromogenic acid-fast bacilli, is described. Both techniques reveal evidence of overlapping of antigens as between mycobacteria belonging to pathogenic and to saprophytic strains.

A STUDY OF VARIOUS FRACTIONS OF BRUCELLA ABORTUS: VI. EXTRACT OF DRIED AND GROUND ORGANISMS

1935 ◽  
Vol 12 (2) ◽  
pp. 161-164
Author(s):  
Ronald Gwatkin
Keyword(s):  
Brucella Abortus ◽  
Guinea Pigs ◽  
Human Subject ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Bacterial Suspension ◽  
Intraperitoneal Route

Filtrates prepared from dry, ground Brucella abortus (Huddleson) caused a fall in temperature and death in some cases in guinea pigs injected by the intraperitoneal route. The filtrates were not as active as bacterial suspension antigen in the complement fixation test. They produced reactions in the skin of infected guinea pigs. Intraperitoneal injections failed to protect guinea pigs against infection by eye with Br. abortus. The preparation of this material gave rise to marked symptoms in a hypersensitive human subject.

A STUDY OF VARIOUS FRACTIONS OF BRUCELLA ABORTUS: V. ALCOHOLIC PRECIPITATES PREPARED FROM A DISSOCIATED STRAIN OF BR. ABORTUS AND FROM E. COLI AND B. SUBTILIS

1935 ◽  
Vol 12 (2) ◽  
pp. 156-160
Author(s):  
Ronald Gwatkin
Keyword(s):  
Guinea Pig ◽  
Brucella Abortus ◽  
Guinea Pigs ◽  
Intraperitoneal Injection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
E Coli

An alcoholic precipitate from an R strain of Brucella abortus resembled the organism from which it was obtained. Toxicity was low, it produced only a slight reaction in the skin of an infected guinea pig and it had no antigenic power in the complement fixation test. An alcoholic precipitate of E. coli was more toxic than any obtained from Br. abortus. The effects of intraperitoneal injections of colon precipitate were modified by anti-colon serum. Intraperitoneal injection of an alcoholic precipitate of B. subtilis produced no change in guinea pigs other than a slight fall in temperature.

Evaluation of the Complement Fixation Test in Amebiasis

1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S113-S133 ◽  
Author(s):  
Sam Brody
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Research Work ◽  
Fixation Test ◽  
Years Of Experience ◽  
Standard Practice ◽  
Clinical Observations ◽  
Technical Procedure ◽  
Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

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