A STUDY OF VARIOUS FRACTIONS OF BRUCELLA ABORTUS: V. ALCOHOLIC PRECIPITATES PREPARED FROM A DISSOCIATED STRAIN OF BR. ABORTUS AND FROM E. COLI AND B. SUBTILIS

1935 ◽  
Vol 12 (2) ◽  
pp. 156-160
Author(s):  
Ronald Gwatkin
Keyword(s):  
Guinea Pig ◽  
Brucella Abortus ◽  
Guinea Pigs ◽  
Intraperitoneal Injection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
E Coli

An alcoholic precipitate from an R strain of Brucella abortus resembled the organism from which it was obtained. Toxicity was low, it produced only a slight reaction in the skin of an infected guinea pig and it had no antigenic power in the complement fixation test. An alcoholic precipitate of E. coli was more toxic than any obtained from Br. abortus. The effects of intraperitoneal injections of colon precipitate were modified by anti-colon serum. Intraperitoneal injection of an alcoholic precipitate of B. subtilis produced no change in guinea pigs other than a slight fall in temperature.

A STUDY OF VARIOUS FRACTIONS OF BRUCELLA ABORTUS: II. COMPLEMENT FIXATION AND INTRADERMAL TESTS WITH ALCOHOLIC PRECIPITATE

1935 ◽  
Vol 12 (1) ◽  
pp. 125-132
Author(s):  
Ronald Gwatkin
Keyword(s):  
Skin Test ◽  
Brucella Abortus ◽  
Guinea Pigs ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Skin Reactions ◽  
Three Samples ◽  
Intradermal Tests

Alcoholic precipitates of Brucella abortus showed high antigenic qualities in the complement fixation test. Dialysis and filtration did not lower this activity. The precipitates gave rise to reactions in infected guinea pigs, in dilutions which produced no change in normal animals. Filtration did not modify the results. In two out of three samples dialysis did not lower the activity of the suspensions as skin test antigens. The addition of formol did not modify the skin reactions.

A STUDY OF VARIOUS FRACTIONS OF BRUCELLA ABORTUS: VI. EXTRACT OF DRIED AND GROUND ORGANISMS

1935 ◽  
Vol 12 (2) ◽  
pp. 161-164
Author(s):  
Ronald Gwatkin
Keyword(s):  
Brucella Abortus ◽  
Guinea Pigs ◽  
Human Subject ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Bacterial Suspension ◽  
Intraperitoneal Route

Filtrates prepared from dry, ground Brucella abortus (Huddleson) caused a fall in temperature and death in some cases in guinea pigs injected by the intraperitoneal route. The filtrates were not as active as bacterial suspension antigen in the complement fixation test. They produced reactions in the skin of infected guinea pigs. Intraperitoneal injections failed to protect guinea pigs against infection by eye with Br. abortus. The preparation of this material gave rise to marked symptoms in a hypersensitive human subject.

scholarly journals Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Immune Responses ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Infected Sheep ◽  
Fixation Test ◽  
Anticomplementary Activity ◽  
Serum Samples ◽  
Saline Extract ◽  
Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

EXPERIENCES WITH A NEW ANTIGEN FOR A COMPLEMENT FIXATION TEST IN TUBERCULOSIS

1955 ◽  
Vol 1 (9) ◽  
pp. 794-798
Author(s):  
N. A. Labzoffsky
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Clinical Improvement ◽  
Ultrasonic Vibration ◽  
Antibody Titer ◽  
Guinea Pigs ◽  
Clinical Cure ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
New Method

A new method for the preparation of complement fixing antigen from Mycobacterium tuberculosis is outlined. The procedure consists of treatment of tubercle bacilli with pyridine, washing the sediment, resuspending it in saline, and finally exposing it to ultrasonic vibration. The antigen so obtained is nonviable, not anticomplementary, and appears to be specific. The results so far obtained with sera from infected guinea pigs and from human tuberculous patients indicate that complement fixing antibodies appear regularly in both. There is a suggestion that in human patients fall in antibody titer is associated with clinical improvement and disappearance with clinical cure.

scholarly journals Detection of Antibodies Brucella abortus in dairy cattle in Puspo District, Pasuruan using Rose Bengal Test and Complement Fixation Test

2021 ◽  
Vol 4 (2) ◽  
pp. 199
Author(s):  
Agung Jati Kusuma ◽  
Erma Safitri ◽  
Ratih Novita Praja ◽  
Wiwiek Tyasningsih ◽  
Maya Nurwartanti Yunita ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Adult Female ◽  
Rose Bengal ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Descriptive Analysis ◽  
Fixation Test ◽  
Analysis Method ◽  
Rose Bengal Test

Brucellosis was an infectious disease caused by the genus brucella. Brucellosis in dairy cattle was caused by Brucella abortus that impaction an abortion. The aim of this study was to determine the presence of antibodies of the Brucella abortus in adult female dairy cattle in the Puspo district Pasuruan using Rose Bengal Test (RBT) and Complement Fixation Test (CFT). A descriptive analysis method with quantitative approach was used in this study. Meanwhile, interviews were conducted to get supporting information from the farmer. Used 100 samples. The results of the study showed 2% samples considered positive brucellosis tested by RBT. To confidence false-positive result, RBT test was followed by CFT. After tested by CFT, in this study there were no brucella antibodies in adult female dairy cattle in Puspo district, Pasuruan.

scholarly journals Seroprevalensi Brucellosis pada Sapi Potong di Papua Barat

2021 ◽  
Vol 2 (1) ◽  
pp. 128-133
Author(s):  
Sulaxono Hadi ◽  
Ratna Loventa Sulaxono
Keyword(s):  
Rose Bengal ◽  
Brucella Abortus ◽  
Screening Test ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Rose Bengal Test ◽  
Papua Barat

Brucellosis merupakan penyakit bakterial yang disebabkan oleh Brucella abortus, yang menga-kibatkan kerugian ekonomi akibat terjadinya keguguran pada sapi betina yang bunting pada triwulan ketiga kebuntingan. Surveilans brucellosis dalam mendukung pengembangan peternakan sapi di Propinsi Papua Barat telah dilakukan oleh Balai Besar Veteriner Maros. Tujuan pelaksanaan surveilans ini adalah untuk mengetahui seroprevalensi brucellosis pada sapi potong di Papua Barat. Surveilans dilakukan di 4 kabupaten/kota, 14 kecamatan dan 33 desa. Sebanyak 684 sampel serum sapi telah diambil guna pengujian terhadap brucellosis. Sampling dilakukan pada lokasi kabupaten, kecamatan dan desa terpilih yang padat ternak. Metode pengujian dilakukan secara seri, dengan melakukan screening test menggunakan Rose Bengal Test, yang bila hasilnya positif diuji lebih lanjut dengan Complement Fixation Test. Hasil pengujian menunjukkan adanya reaktor brucellosis pada 5 ekor dari 684 ekor sapi yang diambil dan diuji serumnya. Seroprevalensi brucellosis pada sapi sebesar 0,73% di desa Remu Utara, kecamatan Sorong, kota Sorong sebanyak 1 ekor serta di desa KaliMerah, kecamatan Masni, kabupaten Manokwari. Untuk mencegah penularan dan penyebaran brucellosis pada sapi maka sapi reaktor brucellosis segera dipotong dengan pengawasan dari petugas.

scholarly journals The conglutination phenomenon: IV. The importance of the choice of complement when examining antisera for the presence of complement-fixing or complement-absorbing antibodies

1948 ◽  
Vol 46 (3) ◽  
pp. 296-310 ◽  
Author(s):  
R. R. A. Coombs ◽  
N. H. Hole
Keyword(s):  
Guinea Pig ◽  
Animal Species ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Serological Diagnosis ◽  
Absorption Test ◽  
Early Results

1. Anti-mallein and anti-typhoid sera produced in various animal species, including man, have been examined for complement-absorbing antibodies using the complements of the pig, horse, cat, man, dog, and guinea-pig, which is possible if use is made of the conglutinating complement-absorption test as well as the haemolytic complement-fixation test.2. Complement-fixing antibodies which are not demonstrable when some complements are used may be detected when other complements are used; for example, antibodies to mallein in human antisera were only detected when the sera were tested with horse and cat complements.3. These early results are published at this stage because of the obvious possible application of these methods in laboratory serological diagnosis. Further work is in progress to elucidate the underlying reasons for these observations.4. The implications of the results are discussed from the practical and theoretical aspects.

scholarly journals Deteksi Brucellosis pada Babi secara Serologis dan Molekuler di Rumah Potong Hewan Kapuk, Jakarta dan Ciroyom, Bandung

2018 ◽  
Vol 5 (2) ◽  
pp. 66-73
Author(s):  
Dina Kartini ◽  
Susan Maphilindawati Noor ◽  
Fachriyan Hasmi Pasaribu
Keyword(s):  
Polymerase Chain Reaction ◽  
Rose Bengal ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Chain Reaction ◽  
Rose Bengal Test ◽  
Brucella Suis ◽  
Polymerase Chain

Brucellosis pada babi merupakan penyakit zoonosis yang disebabkan oleh bakteri Brucella suis. Penyakit ini telah terdeteksi di Indonesia, namun belum banyak diteliti. Pada penelitian ini dilakukan deteksi brucellosis pada babi secara serologis menggunakan metode Rose Bengal Test (RBT) dan Complement Fixation Test (CFT) serta secara molekuler menggunakan teknik Polymerase Chain Reaction (PCR) AMOS untuk mengetahui spesies Brucella yang menginfeksi babi. Sampel penelitian berupa serum dan limfonodus dikoleksi dari 2 Rumah Potong Hewan Kapuk, Jakarta dan Ciroyom, Bandung. Hasil  pengujian secara serologis menunjukkan bahwa brucellosis tidak ditemukan pada sampel babi dari Rumah Potong Hewan Kapuk, Jakarta. Sementara hasil pengujian secara serologis dari Rumah Potong Hewan Ciroyom, Bandung menunjukkan 2,5% (1/40) positif dan 7,5%(3/40) positif PCR. Hasil PCR positif menunjukkan terdeteksi 2 spesies Brucella (B. abortus dan B. suis) pada 2 sampel organ limfonodus dan Brucella suis pada satu sampel limfonodus, sedangkan dari organ limpa terdektesi dua sampel positif Brucella suis. Hal ini menunjukkan bahwa selain Brucella suis, Brucella abortus juga menginfeksi babi pada penelitian ini.

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