A new method for preparing an antigen from kedrowsky's bacillus for the complement fixation test for kala-azar

1962 ◽  
Vol 83 (1) ◽  
pp. 284-287 ◽  
Author(s):  
K. A. Khaleque
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
New Method ◽  
Kala Azar

NEW COMPLEMENT FIXING ANTIGEN FOR SERODIAGNOSIS OF GONORRHOEA

1961 ◽  
Vol 7 (5) ◽  
pp. 715-723
Author(s):  
N. A. Labzoffsky ◽  
A. E. Kelen
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Laboratory Diagnosis ◽  
Fixation Test ◽  
New Method ◽  
Strain Selection ◽  
The Past

A new method of preparing gonococcal antigen for use in the complement fixation test is presented. Briefly, the procedure consists of extracting Neisseria gonorrhoeae cells with pyridine and then exposing the washed sediment to ultrasonic treatment.The new method is superior to that of Price by yielding highly potent, genus-specific and stable antigens, which are free of anticomplementary properties and possess a broader antigenic valence and a longer range of working antigenic power. It is simpler and more practical than Torrey's modification of the Price method since the lengthy and laborious procedure of strain selection is eliminated.In routine use for the past several years, the new antigen has proved to be specific and reliable in the complement fixation test. The test itself provides a useful supplementary aid to the laboratory diagnosis of gonorrhoea.

EXPERIENCES WITH A NEW ANTIGEN FOR A COMPLEMENT FIXATION TEST IN TUBERCULOSIS

1955 ◽  
Vol 1 (9) ◽  
pp. 794-798
Author(s):  
N. A. Labzoffsky
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Clinical Improvement ◽  
Ultrasonic Vibration ◽  
Antibody Titer ◽  
Guinea Pigs ◽  
Clinical Cure ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
New Method

A new method for the preparation of complement fixing antigen from Mycobacterium tuberculosis is outlined. The procedure consists of treatment of tubercle bacilli with pyridine, washing the sediment, resuspending it in saline, and finally exposing it to ultrasonic vibration. The antigen so obtained is nonviable, not anticomplementary, and appears to be specific. The results so far obtained with sera from infected guinea pigs and from human tuberculous patients indicate that complement fixing antibodies appear regularly in both. There is a suggestion that in human patients fall in antibody titer is associated with clinical improvement and disappearance with clinical cure.

Complement Fixation Test in Kala-Azar Using Mycobacterium butyricum Antigen

1958 ◽  
Vol 44 (6) ◽  
pp. 645 ◽  
Author(s):  
J. Pellegrino ◽  
Z. Brener ◽  
Ulysses M. Santos
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Kala Azar

Evaluation of the Complement Fixation Test in Amebiasis

1952 ◽  
Vol 21 (3) ◽  
pp. 391-399 ◽  
Author(s):  
Elwood Buchman ◽  
Harold J. Kullman ◽  
George F. Margonis
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test

IMMUNOASSAY OF GONADOTROPHINS USING COMPLEMENT FIXATION

1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S113-S133 ◽  
Author(s):  
Sam Brody
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Research Work ◽  
Fixation Test ◽  
Years Of Experience ◽  
Standard Practice ◽  
Clinical Observations ◽  
Technical Procedure ◽  
Methodological Problems

ABSTRACT This report is a summary of 10 years of experience with the complement fixation test as adopted for the immunoassay of HCG in serum. It is based on published as well as unpublished material. The discussion centers mainly around methodological problems, criteria of reliability, and clinical observations. It is our impression that the complement fixation test is a reasonably rapid and simple technical procedure. It is standard practice in every bacteriological and virological laboratory. The precision of the HCG assay is high. Its accuracy is good. The complement fixation assay, as reported here, fulfils the criteria of specificity. It has been evaluated by means of serological techniques and through comparison between biopotency and immunopotency of HCG in serum with reference to a common standard. Its application for routine as well as research work is illustrated.

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

Assessment of a complement fixation test to detect Mycoplasma hyopneumoniae infection in pigs

1984 ◽  
Vol 61 (7) ◽  
pp. 216-218
Author(s):  
L. C. LLOYD ◽  
R. T. BADMAN ◽  
J. R. ETHERIDGE ◽  
K. McKECHNIE ◽  
H. IYER
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Mycoplasma Hyopneumoniae ◽  
Fixation Test

Effect of Calcium Ion on the Kolmer Complement-Fixation Test

1954 ◽  
Vol 24 (8) ◽  
pp. 934-945 ◽  
Author(s):  
Alcor S. Browne ◽  
Martha M. Michelbacher ◽  
Edith M. Coffey
Keyword(s):  
Calcium Ion ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test
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