Genome evolution of intermediate wheatgrass as revealed by EST-SSR markers developed from its three progenitor diploid species

Genome ◽  
2015 ◽  
Vol 58 (2) ◽  
pp. 63-70 ◽  
Author(s):  
Richard R.-C. Wang ◽  
Steve R. Larson ◽  
Kevin B. Jensen ◽  
B. Shaun Bushman ◽  
Lee R. DeHaan ◽  
...  
Keyword(s):  
Scientific Literature ◽  
Diploid Species ◽  
Triticum Aestivum L ◽  
Thinopyrum Intermedium ◽  
Forage Crop ◽  
Intermediate Wheatgrass ◽  
Ssr Primers ◽  
Thinopyrum Bessarabicum ◽  
Thinopyrum Elongatum ◽  
Available Information

Intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D.R. Dewey), a segmental autoallohexaploid (2n = 6x = 42), is not only an important forage crop but also a valuable gene reservoir for wheat (Triticum aestivum L.) improvement. Throughout the scientific literature, there continues to be disagreement as to the origin of the different genomes in intermediate wheatgrass. Genotypic data obtained from newly developed EST-SSR primers derived from the putative progenitor diploid species Pseudoroegneria spicata (Pursh) Á. Löve (St genome), Thinopyrum bessarabicum (Savul. & Rayss) Á. Löve (J = Jb = Eb), and Thinopyrum elongatum (Host) D. Dewey (E = Je = Ee) indicate that the V genome of Dasypyrum (Coss. & Durieu) T. Durand is not one of the three genomes in intermediate wheatgrass. Based on all available information in the literature and findings in this study, the genomic designation of intermediate wheatgrass should be changed to JvsJrSt, where Jvs and Jr represent ancestral genomes of present-day Jb of Th. bessarabicum and Je of Th. elongatum, with Jvs being more ancient. Furthermore, the information suggests that the St genome in intermediate wheatgrass is most similar to the present-day St found in diploid species of Pseudoroegneria from Eurasia.

Differential transferability of EST-SSR primers developed from the diploid species Pseudoroegneria spicata, Thinopyrum bessarabicum, and Thinopyrum elongatum

Genome ◽  
2017 ◽  
Vol 60 (6) ◽  
pp. 530-536 ◽  
Author(s):  
Richard R.-C. Wang ◽  
Steve R. Larson ◽  
Kevin B. Jensen
Keyword(s):  
Expressed Sequence Tag ◽  
Genome Mapping ◽  
Diploid Species ◽  
Genetic Maps ◽  
Pseudoroegneria Spicata ◽  
Ssr Primers ◽  
Polymorphic Species ◽  
Thinopyrum Bessarabicum ◽  
Species Specific ◽  
Thinopyrum Elongatum

Simple sequence repeat technology based on expressed sequence tag (EST-SSR) is a useful genomic tool for genome mapping, characterizing plant species relationships, elucidating genome evolution, and tracing genes on alien chromosome segments. EST-SSR primers developed from three perennial diploid species of Triticeae, Pseudoroegneria spicata (Pursh) Á. Löve (having St genome), Thinopyrum bessarabicum (Savul. & Rayss) Á. Löve (Jb = Eb = J), and Thinopyrum elongatum (Host) D.R. Dewey (Je = Ee = E), were used to produce amplicons in these three species to (i) assess relative transferability, (ii) identify polymorphic species-specific markers, and (iii) determine genome relationships among the three species. Because of the close relationship between Jb and Je genomes, EST-SSR primers derived from Th. bessarabicum and Th. elongatum had greater transferability to each other than those derived from the St-genome P. spicata. A large number of polymorphic species- and genome-specific EST-SSR amplicons were identified that will be used for construction of genetic maps of these diploid species, and tracing economically useful genes in breeding or gene transfer programs in various species of Triticeae.

Dilemma of genome relationship in the diploid species Thinopyrum bessarabicum and Thinopyrum elongatum (Triticeae: Poaceae)

Genome ◽  
1990 ◽  
Vol 33 (6) ◽  
pp. 944-946 ◽  
Author(s):  
Prem P. Jauhar
Keyword(s):  
Chromosome Pairing ◽  
Constitutive Heterochromatin ◽  
Diploid Species ◽  
Total Content ◽  
Ploidy Levels ◽  
5S Dna ◽  
Thinopyrum Bessarabicum ◽  
Thinopyrum Elongatum ◽  
Relationship Of ◽  
The Relationship

Evidence on the relationship of the J genome of diploid Thinopyrum bessarabicum and the E genome of diploid Thinopyrum elongatum (= Lophopyrum elongatum) is discussed. Low chromosome pairing between J and E at different ploidy levels, suppression of J–E pairing by the Ph1 pairing regulator that inhibits homoeologous pairing, complete sterility of the diploid hybrids (JE), karyotypic differentiation of the two genomes and differences in their biochemical organization as reflected in total content and distribution of constitutive heterochromatin, and marked differences in isozymes, 5S DNA, and rDNA indicate that J and E are distinct genomes. These genomes are homoeologous and not homologous. There is no justification for the merger of J and E genomes.Key words: chromosome pairing, Ph1 pairing regulator, C-banding, isozymes, 5S DNA, rDNA.

scholarly journals Evaluating Wheat Microsatellite Markers for the Use in Genetic Analysis of Thinopyrum, Dasypyrum, and Pseudoroegneria Species

2013 ◽  
Vol 2013 ◽  
pp. 1-3 ◽  
Author(s):  
Pavel Yu. Kroupin ◽  
Mikhail G. Divashuk ◽  
Igor A. Fesenko ◽  
Gennady I. Karlov
Keyword(s):  
Genetic Analysis ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Wild Relatives ◽  
Wheat Genome ◽  
Dasypyrum Villosum ◽  
Thinopyrum Intermedium ◽  
Phylogenetic Studies ◽  
Thinopyrum Bessarabicum ◽  
Thinopyrum Elongatum

A set of 42 SSRs of wheat were evaluated for their cross-amplification on the DNA of Thinopyrum ponticum, Thinopyrum intermedium, Thinopyrum elongatum, Thinopyrum bessarabicum, Pseudoroegneria stipifolia, and Dasypyrum villosum. The number of the wheat SSR markers that amplified DNA fragments with determined size for Th. ponticum was 33 (78.6%); for Th. intermedium, 28 (66.7%); for Th. elongatum, 24 (57.1%); for Th. bessarabicum, 24 (57.1%); for P. stipifolia, 26 (69.1%); and for D. villosum, 29 (69.0%). Twenty-four primer pairs of wheat SSR markers were successfully amplified from all investigated species. The dataset can be used for phylogenetic studies of wild relatives of wheat, for the estimation of their diversity, and for the introgression of agronomically valuable genes into wheat genome.

Characterization of six wheat × Thinopyrum intermedium derivatives by GISH, RFLP, and multicolor GISH

Genome ◽  
2003 ◽  
Vol 46 (3) ◽  
pp. 490-495 ◽  
Author(s):  
F P Han ◽  
G Fedak ◽  
A Benabdelmouna ◽  
K Armstrong ◽  
T Ouellet
Keyword(s):  
Genomic Dna ◽  
Aegilops Tauschii ◽  
Rflp Analysis ◽  
Aegilops Speltoides ◽  
Thinopyrum Intermedium ◽  
B Genome ◽  
A Genome ◽  
Genetic Stocks ◽  
Disomic Addition Lines ◽  
Thinopyrum Elongatum

Restriction fragment length polymorphism (RFLP) analysis and multicolor genomic in situ hybridization (GISH) are useful tools to precisely characterize genetic stocks derived from crosses of wheat (Triticum aestivum) with Thinopyrum intermedium and Thinopyrum elongatum. The wheat × Th. intermedium derived stocks designated Z1, Z2, Z3, Z4, Z5, and Z6 were initially screened by multicolor GISH using Aegilops speltoides genomic DNA for blocking and various combinations of genomic DNA from Th. intermedium, Triticum urartu, and Aegilops tauschii for probes. The probing (GISH) results indicated that lines Z1 and Z3 were alien disomic addition lines with chromosome numbers of 2n = 44. Z2 was a substitution line in which chromosome 2D was substituted by a pair of Th. intermedium chromosomes; this was confirmed by RFLP and muticolour GISH. Z4 (2n = 44) contained two pairs of wheat – Th. intermedium translocated chromosomes; one pair involved A-genome chromosomes, the other involved D- and A-genome chromosomes. Z5 (2n = 44) contained one pair of wheat – Th. intermedium translocated chromosomes involving the D- and A-genome chromosomes of wheat. Z6 (2n = 44) contained one pair of chromosomes derived from Th. intermedium plus another pair of translocated chromosomes involving B-genome chromosomes of wheat. Line Z2 was of special interest because it has some resistance to infection by Fusarium graminearum.Key words: wheat, Thinopyrum intermedium, addition, substitution, and translocation lines, GISH, multicolor GISH, RFLP.

AN EVALUATION OF ALTAI WILD RYE, ELYMUS ANGUSTUS TRIN., AS A FORAGE CROP

1960 ◽  
Vol 40 (2) ◽  
pp. 295-305 ◽  
Author(s):  
T. Lawrence ◽  
D. H. Heinrichs ◽  
R. B. Carson
Keyword(s):  
Crude Protein ◽  
Nutritive Value ◽  
The Other ◽  
Crude Protein Content ◽  
Forage Crops ◽  
Crested Wheatgrass ◽  
Forage Crop ◽  
Intermediate Wheatgrass ◽  
Russian Wild Rye ◽  
Better Than

A study was conducted with Altai wild rye (Elymus angustus Trin.), Russian wild rye (Elymus junceus), crested wheatgrass (Agropyron cristatum), and intermediate wheatgrass (Agropyron intermedium) on their relative merits as forage crops in the arid climate of Saskatchewan. The data show that Altai wild rye produced as much forage as Russian wild rye but less than crested wheatgrass and intermediate wheatgrass. However, it appeared to be less competitive with alfalfa than the other grasses in the test. The nutritive value of Altai wild rye was as good as or better than that of the other grasses; it excelled all of them in crude protein content throughout the season and contained about the same amount of fibre and fat at most stages of development; its ash content was higher than that of the other three grasses in 1954, a wet year, but only higher than the two wheatgrasses in 1957, a very dry year. Comparisons regarding palatability and digestibility suggest that Altai wild rye equals the better grasses in this respect. The study also indicates that nutritive value of grasses throughout the growing season varies much more in wet years when normal plant development occurs, than in dry years when growth is slow and sporadic.

Structural characterization of intermediate wheatgrass ( Thinopyrum intermedium ) starch

2019 ◽  
Vol 96 (5) ◽  
pp. 927-936
Author(s):  
Yingxin Zhong ◽  
Juan Mogoginta ◽  
Joseph Gayin ◽  
George Amponsah Annor
Keyword(s):  
Structural Characterization ◽  
Thinopyrum Intermedium ◽  
Intermediate Wheatgrass

scholarly journals Utilization of Natural History Information in Evidence based Herpetoculture: A Proposed Protocol and Case Study with Hydrodynastes gigas (False Water Cobra)

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2021
Author(s):  
Zachary J. Loughman
Keyword(s):  
Natural History ◽  
Captive Breeding ◽  
Scientific Literature ◽  
Evidence Based ◽  
Scientific Inference ◽  
New Information ◽  
History Information ◽  
History Of ◽  
Available Information

Herpetocultural practices are based on norms driven by economy of space and time for keepers, with little scientific inference backing their practice. In recent years, a subset of herpetoculturalists have promoted evidence-based husbandry that relies on science and experimental design to generate husbandry practice. A theoretical framework and protocol are proposed herein that enables any individual who has access to the internet the ability to use various outlets of natural history information (scientific literature databases, social media sources, and weather websites) and previously published husbandry reports as evidence to drive the creation of novel herpetocultural practice. A case study is provided which compares readily available information on the care of Hydrodynastes gigas (false water cobra), such as online care sheets for the species, with the proposed evidence based herpetocultural protocol founded on natural history information and published care and captive breeding reports. Results were assessed for protocol efficacy and determined that the natural history informed evidence-based approach increased animal welfare and generated new information specific to the natural history of H. gigas.

scholarly journals Effect of Radio Frequency Cold Plasma Treatment on Intermediate Wheatgrass (Thinopyrum intermedium) Flour and Dough Properties in Comparison to Hard and Soft Wheat (Triticum aestivum L.)

2019 ◽  
Vol 2019 ◽  
pp. 1-8 ◽  
Author(s):  
Sophie Held ◽  
Catrin E. Tyl ◽  
George A. Annor
Keyword(s):  
Plasma Treatment ◽  
Cold Plasma ◽  
Soft Wheat ◽  
Thinopyrum Intermedium ◽  
Retention Capacity ◽  
Intermediate Wheatgrass ◽  
Hard Wheat ◽  
Protein Secondary Structures ◽  
Solvent Retention ◽  
Solvent Retention Capacity

Cold plasma is an emerging technology to improve microbiological safety as well as functionality of foods. This study compared the effect of radio frequency cold plasma on flour and dough properties of three members of the Triticeae tribe, soft as well as hard wheat (Triticum aestivum L.) and intermediate wheatgrass (Thinopyrum intermedium, IWG). These three flour types differ in their protein content and composition and were evaluated for their solubility, solvent retention capacity, starch damage, GlutoPeak and Farinograph profiles, and protein secondary structures. Plasma treatment resulted in dehydration of flours but did not change protein content or solubility. Farinograph water absorption increased for all flours after plasma treatment (from 56.5–61.1 before to 71.0–81.6%) and coincided with higher solvent retention capacity for water and sodium carbonate. Plasma treatment under our conditions was found to cause starch damage to the extent of 3.46–6.62% in all samples, explaining the higher solvent retention capacity for sodium carbonate. However, Farinograph properties were changed differently in each flour type: dough development time and stability time decreased for hard wheat and increased for soft wheat but remained unchanged in intermediate wheatgrass. GlutoPeak parameters were also affected differently: peak torque for intermediate wheatgrass increased from 32 to 39.5 GlutoPeak units but was not different for the other two flours. Soft wheat did not always aggregate after plasma treatment, i.e., did not aggregate within the measurement time. It was also the only flour where protein secondary structures were changed after plasma treatment, exhibiting an increase from 15.2 to 27.9% in β-turns and a decrease from 59.4 to 47.9% in β-sheets. While this could be indicative of a better hydrated gluten network, plasma-treated soft wheat was the only flour where viscoelastic properties were changed and extensibility decreased. Further research is warranted to elucidate molecular changes underlying these effects.

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