Combined AFLP and RFLP mapping in two hexaploid oat recombinant inbred populations

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 94-101 ◽  
Author(s):  
Hua Jin ◽  
Leslie L Domier ◽  
Xuejen Shen ◽  
Frederic L Kolb
Keyword(s):  
Linkage Map ◽  
Recombinant Inbred ◽  
Barley Yellow Dwarf Virus ◽  
Aflp Markers ◽  
Rflp Markers ◽  
Linkage Groups ◽  
Data Set ◽  
Rflp Map ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

A combined RFLP and AFLP map was constructed for hexaploid oat (Avena spp.). The segregation of AFLP markers was scored in two hexaploid oat recombinant inbred line (RIL) populations, the 'Kanota' × 'Ogle' RFLP population, and a population derived from 'Clintland64' and 'IL86-5698', barley yellow dwarf virus (BYDV)-sensitive and BYDV-tolerant lines, respectively. More than 300 AFLP markers were scored in each population, of which 97 could be scored in both populations. AFLP markers were linked to RFLP markers in 32 of 36 'Kanota' × 'Ogle' RFLP linkage groups. The addition of the AFLP markers to the 'Kanota' × 'Ogle' RFLP data set combined markers from four pairs of linkage groups and increased the size of the map from 1402 cM to 2351 cM. Thirty linkage groups were observed in the 'Clintland64' × 'IL86-5698' population, two of which could be consolidated by comparing the maps from both populations. The AFLP and RFLP markers showed very similar distributions in the 'Kanota' × 'Ogle' population with a tendency of each type of marker to cluster with markers of the same type. The placement of a set of AFLP markers on the 'Kanota' × 'Ogle' linkage map will enrich the RFLP map and allow others to relate AFLP markers for agronomically important genes to the reference 'Kanota' × 'Ogle' linkage map. Key words: amplified fragment length polymorphism, Avena, comparative mapping.

Two genetic linkage maps of mungbean using RFLP and RAPD markers

2000 ◽  
Vol 51 (4) ◽  
pp. 415 ◽  
Author(s):  
C. J. Lambrides ◽  
R. J. Lawn ◽  
I. D. Godwin ◽  
J. Manners ◽  
B. C. Imrie
Keyword(s):  
Linkage Map ◽  
Segregation Distortion ◽  
Recombinant Inbred ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Rflp Markers ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Genetic Linkage Maps ◽  
Map Distance

Two genetic linkage maps of mungbean derived from the cross Berken ACC 41 are reported. The F2 map constructed from 67 individuals consisted of 110 markers (52 RFLP and 56 RAPD) that grouped into 12 linkage groups. The linked markers spanned a total map distance of 758.3 cM. A recombinant inbred (RI) population derived from the 67 F2 individuals was used for the generation of an additional linkage map. The RI map, composed entirely of RAPD markers, consisted of 115 markers in 12 linkage groups. The linked markers spanned a total map distance of 691.7 cM. Using a framework set of RFLP markers, the F2 map was compared with another F2 mungbean map constructed in Minnesota. In general, the order of these markers was consistent between maps. Segregation distortion was observed for some markers. 14.5% (16/110) of mapped F2 markers and 24% (28/115) of mapped RI markers segregated with distorted ratios. Segregation distortion occurred in each successive generation after the F2 . The regions of distortion identified in the Australian maps did not coincide with regions of the Minnesota map.

RFLP linkage map and genome analysis of Saccharum spontaneum

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 782-791 ◽  
Author(s):  
Jorge A. G. da Silva ◽  
Mark E. Sorrells ◽  
William L Burnquist ◽  
Steven D. Tanksley
Keyword(s):  
Linkage Map ◽  
Genome Size ◽  
Genome Analysis ◽  
Dna Probes ◽  
Rflp Markers ◽  
Saccharum Spontaneum ◽  
Linkage Groups ◽  
Rflp Map ◽  
Dna Libraries ◽  
Rice Cdna

An RFLP linkage map of the wild sugarcane species Saccharum spontaneum L. (2n = 8x = 40–128) was constructed, comprising 216 loci, detected by 116 DNA probes, and distributed over 44 linkage groups. At a density of at least one marker every 25-cM interval, the coverage of the genome was estimated as 86%. For the generation of RFLP markers, probes were surveyed from seven DNA libraries: three sugarcane cDNA, one oat cDNA, one rice cDNA, and one barley cDNA, as well as one sugarcane genomic. Sixty-two maize genomic clones that were previously mapped on maize were used to initiate a comparative map between the sugarcane, sorghum, and maize genomes. Based on the RFLP segregation data, we conclude that this species is an autopolyploid, with an estimated genome size of 2107 cM.Key words: sugarcane, polyploid, RFLP, map, genome.

scholarly journals Identification of Quantitative Loci for Tolerance to Barley Yellow Dwarf Virus in Oat

1998 ◽  
Vol 88 (5) ◽  
pp. 410-415 ◽  
Author(s):  
Hua Jin ◽  
Leslie L. Domier ◽  
Frederic L. Kolb ◽  
Charles M. Brown
Keyword(s):  
Length Polymorphism ◽  
Fragment Length ◽  
Fragment Length Polymorphism ◽  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Linkage Groups ◽  
Barley Yellow Dwarf ◽  
Ril Population ◽  
Yellow Dwarf Virus

Molecular markers linked to quantitative trait loci conditioning tolerance to barley yellow dwarf virus (BYDV) were identified in oat (Avena sativa) using amplified fragment length polymorphism (AFLP) analysis. Near-isogenic and recombinant inbred lines (NILs and RILs, respectively) derived from a cross of Clintland64 (BYDV-sensitive) and IL86-5698 (BYDV-tolerant) were evaluated for their responses to an Illinois isolate of the PAV strain of BYDV. Individual markers identified in the analysis of the NILs explained up to 35% of the variability seen in the tolerance response. Single-point analysis of the marker data from the RIL population identified 24 markers in three linkage groups that were associated with tolerance to BYDV infection at P ≤ 0.001. These markers defined three major loci, A, C, and E, that were contributed by the tolerant parent (IL86-5698) and explained 35.0, 20.6, and 17.0% of the variability, respectively. Three minor loci G, H1, and R) were identified at P ≤ 0.01. These loci were contributed by the sensitive parent (Clintland64) and explained 5.8, 5.6, and 5.6% of the variability, respectively. Interval analysis showed that only the loci A, C, and E are associated significantly with BYDV tolerance at log of the likelihood ratio ≥ 3.0. These loci explained about 50% total of the variation in BYDV tolerance in multimarker regression analysis in both years. The BYDV tolerance loci A, C, E, and R were mapped to hexaploid oat restriction fragment length polymorphism linkage groups 2, 8, 36, and 5, respectively, by analyzing the segregation of the AFLP markers in the Kanota × Ogle RIL population.

Molecular characterization of a Thinopyrum intermedium Group 2 chromosome (2Ai-2) conferring resistance to barley yellow dwarf virus

Genome ◽  
2001 ◽  
Vol 44 (6) ◽  
pp. 1129-1135 ◽  
Author(s):  
Z Y Zhang ◽  
Z Y Xin ◽  
P J Larkin
Keyword(s):  
Barley Yellow Dwarf Virus ◽  
Rapd Marker ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Rflp Markers ◽  
Thinopyrum Intermedium ◽  
Barley Yellow Dwarf ◽  
Wheat Group ◽  
Group 2 ◽  
Yellow Dwarf Virus

The wheat – Thinopyrum intermedium addition lines Z1 and Z2 carry 21 pairs of wheat chromosomes and one pair of Th. intermedium chromosomes (2Ai-2) conferring resistance to barley yellow dwarf virus (BYDV). GISH results using the genomic DNA of Pseudoroegneria strigosa (S genome) as the probe indicated that the 2Ai-2 chromosome in Z1 and Z2 is an S–J intercalary translocation. Most of the 2Ai-2 chromosome belongs to the S genome, except for about one third in the middle region of the long arm that belongs to the J genome. The results of detailed RFLP analyses confirmed that the 2Ai-2 chromosome is extensively homoeologous to wheat group 2 chromosomes. Some new RFLP markers specific to the 2Ai-2 chromosome were identified. A RAPD marker, OP-R16340, specific to the 2Ai-2 chromosome, was screened. We converted the RAPD marker into a sequence-characterized amplified region (SCAR) marker (designated SC-R16). The study establishes the basis for selecting translocation lines with small segments of the 2Ai-2 chromosome and localizing the BYDV resistance gene when introgressed into a wheat background.Key words: Thinopyrum intermedium, barley yellow dwarf virus (BYDV), genomic in situ hybridization (GISH), RFLP, SCAR, homoeologous group 2.

scholarly journals 071 A Saturated Linkage Map for the Garden Pea

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 453D-453
Author(s):  
Norman Weeden ◽  
Gail Timmerman-Vaughan
Keyword(s):  
Linkage Map ◽  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Inbred Lines ◽  
Aflp Markers ◽  
Linkage Groups ◽  
Garden Pea ◽  
Molecular Studies ◽  
Firm Basis ◽  
Translocation Heterozygosity

A linkage map for a set of 51 F2-derived recombinant inbred lines has been constructed from the segregation data of ≈850 morphological, isozyme, RFLP, STS, RAPD, and AFLP markers. The final map consists of seven clear linkage groups with a total length of nearly 900 cM. The wide variety of loci placed on this map permits its comparison with partial maps that have been developed in other programs. For the most part, the arrangement of loci agrees with that in previous maps, and no evidence for translocation heterozygosity in this cross is apparent. Although some clustering of markers is observed, for the most part the markers are well-distributed, and few gaps greater than 5 cM are found in the coverage. The availability of this first “complete” and highly saturated map for pea should permit more efficient comparison of the partial maps that have been generated in a number of different crosses, as well as provide a firm basis for future mapping and molecular studies in this species.

Construction of a sorghum RFLP linkage map using sorghum and maize DNA probes

Genome ◽  
1994 ◽  
Vol 37 (4) ◽  
pp. 590-594 ◽  
Author(s):  
R. A. Ragab ◽  
S. Dronavalli ◽  
M. A. Saghai Maroof ◽  
Y. G. Yu
Keyword(s):  
Linkage Map ◽  
Fragment Length Polymorphism ◽  
Genomic Library ◽  
Restriction Enzymes ◽  
Rflp Markers ◽  
Linkage Groups ◽  
Rflp Map ◽  
Hybridization Probes ◽  
Low Copy Number ◽  
Marker Distance

Previous reports on sorghum restriction fragment length polymorphism (RFLP) mapping have been limited to the use of heterologous maize clones. In this study, both sorghum and maize probes were used to construct an RFLP map based on an F2 population from a cross between sorghum lines BSC 35 and BTX 631. A set of single-or low-copy number clones from a sorghum genomic library was preselected to use as hybridization probes. Forty-nine of the 101 clones (49%) tested were polymorphic between the two parental lines. In comparison, 53 of the 135 maize probes (39%) detected polymorphism with the same restriction enzymes. In total, 71 RFLP markers (38 sorghum and 33 maize) were placed into 15 linkage groups spanning 633 cM with an average marker distance of 8.9 cM. Comparison of our linkage map with other published sorghum maps, based on maize probes, showed resemblance for several linkage groups, indicating that these maps can be integrated. Homologous sorghum probes are useful in improving the coverage and resolution of RFLP linkage map in sorghum.Key words: RFLP, Sorghum bicolor, chromosomes, Zea mays.

Tolerance to Barley Yellow Dwarf Virus in Triticale

Crop Science ◽  
1990 ◽  
Vol 30 (5) ◽  
pp. 1008 ◽  
Author(s):  
J. Collin ◽  
A. Comeau ◽  
C.A. St-Pierre
Keyword(s):  
Barley Yellow Dwarf Virus ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus
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