Construction of a sorghum RFLP linkage map using sorghum and maize DNA probes

Genome ◽  
1994 ◽  
Vol 37 (4) ◽  
pp. 590-594 ◽  
Author(s):  
R. A. Ragab ◽  
S. Dronavalli ◽  
M. A. Saghai Maroof ◽  
Y. G. Yu
Keyword(s):  
Linkage Map ◽  
Fragment Length Polymorphism ◽  
Genomic Library ◽  
Restriction Enzymes ◽  
Rflp Markers ◽  
Linkage Groups ◽  
Rflp Map ◽  
Hybridization Probes ◽  
Low Copy Number ◽  
Marker Distance

Previous reports on sorghum restriction fragment length polymorphism (RFLP) mapping have been limited to the use of heterologous maize clones. In this study, both sorghum and maize probes were used to construct an RFLP map based on an F2 population from a cross between sorghum lines BSC 35 and BTX 631. A set of single-or low-copy number clones from a sorghum genomic library was preselected to use as hybridization probes. Forty-nine of the 101 clones (49%) tested were polymorphic between the two parental lines. In comparison, 53 of the 135 maize probes (39%) detected polymorphism with the same restriction enzymes. In total, 71 RFLP markers (38 sorghum and 33 maize) were placed into 15 linkage groups spanning 633 cM with an average marker distance of 8.9 cM. Comparison of our linkage map with other published sorghum maps, based on maize probes, showed resemblance for several linkage groups, indicating that these maps can be integrated. Homologous sorghum probes are useful in improving the coverage and resolution of RFLP linkage map in sorghum.Key words: RFLP, Sorghum bicolor, chromosomes, Zea mays.

RFLP linkage map and genome analysis of Saccharum spontaneum

Genome ◽  
1993 ◽  
Vol 36 (4) ◽  
pp. 782-791 ◽  
Author(s):  
Jorge A. G. da Silva ◽  
Mark E. Sorrells ◽  
William L Burnquist ◽  
Steven D. Tanksley
Keyword(s):  
Linkage Map ◽  
Genome Size ◽  
Genome Analysis ◽  
Dna Probes ◽  
Rflp Markers ◽  
Saccharum Spontaneum ◽  
Linkage Groups ◽  
Rflp Map ◽  
Dna Libraries ◽  
Rice Cdna

An RFLP linkage map of the wild sugarcane species Saccharum spontaneum L. (2n = 8x = 40–128) was constructed, comprising 216 loci, detected by 116 DNA probes, and distributed over 44 linkage groups. At a density of at least one marker every 25-cM interval, the coverage of the genome was estimated as 86%. For the generation of RFLP markers, probes were surveyed from seven DNA libraries: three sugarcane cDNA, one oat cDNA, one rice cDNA, and one barley cDNA, as well as one sugarcane genomic. Sixty-two maize genomic clones that were previously mapped on maize were used to initiate a comparative map between the sugarcane, sorghum, and maize genomes. Based on the RFLP segregation data, we conclude that this species is an autopolyploid, with an estimated genome size of 2107 cM.Key words: sugarcane, polyploid, RFLP, map, genome.

A core linkage map of the bumblebee Bombus terrestris

Genome ◽  
2006 ◽  
Vol 49 (10) ◽  
pp. 1215-1226 ◽  
Author(s):  
Lena Wilfert ◽  
Jürgen Gadau ◽  
Paul Schmid-Hempel
Keyword(s):  
Linkage Map ◽  
Fragment Length Polymorphism ◽  
Bombus Terrestris ◽  
Linkage Groups ◽  
Haploid Chromosome Number ◽  
Genetic Linkage Maps ◽  
Marker Distance ◽  
Core Linkage Map ◽  
Mapping Populations ◽  
Reference Tool

The bumblebee Bombus terrestris is an economically important pollinator and an emerging model species in quantitative and population genetics. We generated genetic linkage maps for 3 independent mapping populations of B. terrestris. The linkage map with the highest resolution had 21 linkage groups, which adequately represents the haploid chromosome number of B. terrestris (n = 18). This map can be considered saturated, with an average marker distance of 10.3 cM and an estimated genome coverage of 81%. Using flow cytometry, we have estimated the genome size of this species to be 625 Mb. With an estimated total recombination genome length of 2760 cM, this results in a ratio of 226 kb/cM between the physical and genetic genome sizes. A recurring set of microsatellites and amplified fragment length polymorphism (AFLP) markers allowed the alignment of 14 linkage groups between the 3 maps. We propose to adopt this core map as a reference tool for future genetic and molecular work in B. terrestris.

Combined AFLP and RFLP mapping in two hexaploid oat recombinant inbred populations

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 94-101 ◽  
Author(s):  
Hua Jin ◽  
Leslie L Domier ◽  
Xuejen Shen ◽  
Frederic L Kolb
Keyword(s):  
Linkage Map ◽  
Recombinant Inbred ◽  
Barley Yellow Dwarf Virus ◽  
Aflp Markers ◽  
Rflp Markers ◽  
Linkage Groups ◽  
Data Set ◽  
Rflp Map ◽  
Barley Yellow Dwarf ◽  
Yellow Dwarf Virus

A combined RFLP and AFLP map was constructed for hexaploid oat (Avena spp.). The segregation of AFLP markers was scored in two hexaploid oat recombinant inbred line (RIL) populations, the 'Kanota' × 'Ogle' RFLP population, and a population derived from 'Clintland64' and 'IL86-5698', barley yellow dwarf virus (BYDV)-sensitive and BYDV-tolerant lines, respectively. More than 300 AFLP markers were scored in each population, of which 97 could be scored in both populations. AFLP markers were linked to RFLP markers in 32 of 36 'Kanota' × 'Ogle' RFLP linkage groups. The addition of the AFLP markers to the 'Kanota' × 'Ogle' RFLP data set combined markers from four pairs of linkage groups and increased the size of the map from 1402 cM to 2351 cM. Thirty linkage groups were observed in the 'Clintland64' × 'IL86-5698' population, two of which could be consolidated by comparing the maps from both populations. The AFLP and RFLP markers showed very similar distributions in the 'Kanota' × 'Ogle' population with a tendency of each type of marker to cluster with markers of the same type. The placement of a set of AFLP markers on the 'Kanota' × 'Ogle' linkage map will enrich the RFLP map and allow others to relate AFLP markers for agronomically important genes to the reference 'Kanota' × 'Ogle' linkage map. Key words: amplified fragment length polymorphism, Avena, comparative mapping.

scholarly journals 674 PB 154 DEVELOPMENT OF AN RFLP MAP FOR WALNUT

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 529d-529
Author(s):  
Robert G. Fjellstrom ◽  
Dan E. Parfitt
Keyword(s):  
Copy Number ◽  
Genomic Dna ◽  
Genomic Library ◽  
Backcross Progeny ◽  
The Other ◽  
Probability Method ◽  
Genome Length ◽  
Linkage Groups ◽  
Rflp Map ◽  
Low Copy Number

32 cloned probes from a walnut (Juglans sp.) PstI random genomic library were used to develop a linkage map for walnut. Low copy number walnut random genomic DNA probes were constructed and hybridized to restriction endonuclease digested DNA from parent walnut trees from a backcross of (J. hindsii × J. regia) with J. regia to identify parental polymorphism. 63 backcross progeny were analyzed to determine the inheritance and linkage of 48 RFLP loci. 66% of the probes detected duplicated, but unlinked loci. 42 of the RFLP loci could be placed on 12 linkage groups. The other 6 loci could not be placed on common linkage groups. (Theoretical maximum number of linkage groups is 16.) A Poisson probability method for estimating genome size was utilized to calculate the approximate walnut genome length as 1660 cm and to estimate that 138 markers would be needed to cover 95% the walnut genome within 20 cM of each marker.

scholarly journals A Genetic Map of Gibberella fujikuroi Mating Population A (Fusarium moniliforme)

Genetics ◽  
1996 ◽  
Vol 143 (1) ◽  
pp. 175-189 ◽  
Author(s):  
Jin-rong Xu ◽  
John F Leslie
Keyword(s):  
Fusarium Moniliforme ◽  
Fragment Length Polymorphism ◽  
Fumonisin B1 ◽  
Gibberella Fujikuroi ◽  
Female Sterility ◽  
Rflp Markers ◽  
Linkage Groups ◽  
Mating Population ◽  
Spore Killer ◽  
Chiasma Interference

Abstract We constructed a recombination-based map of the fungal plant pathogen Gibberella fujikuroi mating population A (asexual stage Fusarium moniliforme). The map is based on the segregation of 142 restriction fragment length polymorphism (RFLP) markers, two auxotrophic genes (arg1, nic1), mating type (matA+ / matA−), female sterility (ste1), spore-killer (Sk), and a gene governing the production of the mycotoxin fumonisin B1 (fum1) among 121 random ascospore progeny from a single cross. We identified 12 linkage groups corresponding to the 12 chromosome-sized DNAs previously observed in contour-clamped homogeneous electric field (CHEF) gels. Linkage groups and chromosomes were correlated via Southern blots between appropriate RFLP markers and the CHEF gels. Eleven of the 12 chromosomes are meiotically stable, but the 12th (and smallest) is subject to deletions in 3% (4/121) of the progeny. Positive chiasma interference occurred on five of the 12 chromosomes, and nine of the 12 chromosomes averaged more than one crossover per chromosome. The average kb/cM ratio in this cross is ~32.

Two genetic linkage maps of mungbean using RFLP and RAPD markers

2000 ◽  
Vol 51 (4) ◽  
pp. 415 ◽  
Author(s):  
C. J. Lambrides ◽  
R. J. Lawn ◽  
I. D. Godwin ◽  
J. Manners ◽  
B. C. Imrie
Keyword(s):  
Linkage Map ◽  
Segregation Distortion ◽  
Recombinant Inbred ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Rflp Markers ◽  
Linkage Maps ◽  
Linkage Groups ◽  
Genetic Linkage Maps ◽  
Map Distance

Two genetic linkage maps of mungbean derived from the cross Berken ACC 41 are reported. The F2 map constructed from 67 individuals consisted of 110 markers (52 RFLP and 56 RAPD) that grouped into 12 linkage groups. The linked markers spanned a total map distance of 758.3 cM. A recombinant inbred (RI) population derived from the 67 F2 individuals was used for the generation of an additional linkage map. The RI map, composed entirely of RAPD markers, consisted of 115 markers in 12 linkage groups. The linked markers spanned a total map distance of 691.7 cM. Using a framework set of RFLP markers, the F2 map was compared with another F2 mungbean map constructed in Minnesota. In general, the order of these markers was consistent between maps. Segregation distortion was observed for some markers. 14.5% (16/110) of mapped F2 markers and 24% (28/115) of mapped RI markers segregated with distorted ratios. Segregation distortion occurred in each successive generation after the F2 . The regions of distortion identified in the Australian maps did not coincide with regions of the Minnesota map.

scholarly journals Genetic Linkage Map of the Edible BasidiomycetePleurotus ostreatus

2000 ◽  
Vol 66 (12) ◽  
pp. 5290-5300 ◽  
Author(s):  
Luis M. Larraya ◽  
G�mer P�rez ◽  
Enrique Ritter ◽  
Antonio G. Pisabarro ◽  
Lucı́a Ramı́rez
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Gene Sequence ◽  
Genetic Linkage Map ◽  
Fragment Length Polymorphism ◽  
Individual Cell ◽  
Random Amplified Polymorphic Dna ◽  
Rrna Gene ◽  
Linkage Groups ◽  
Rrna Gene Sequence

ABSTRACT We have constructed a genetic linkage map of the edible basidiomycete Pleurotus ostreatus (var. Florida). The map is based on the segregation of 178 random amplified polymorphic DNA and 23 restriction fragment length polymorphism markers; four hydrophobin, two laccase, and two manganese peroxidase genes; both mating type loci; one isozyme locus (est1); the rRNA gene sequence; and a repetitive DNA sequence in a population of 80 sibling monokaryons. The map identifies 11 linkage groups corresponding to the chromosomes ofP. ostreatus, and it has a total length of 1,000.7 centimorgans (cM) with an average of 35.1 kbp/cM. The map shows a high correlation (0.76) between physical and genetic chromosome sizes. The number of crossovers observed per chromosome per individual cell is 0.89. This map covers nearly the whole genome of P. ostreatus.

scholarly journals RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN GENETICALLY RELATED ROSE CULTIVARS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 574d-574
Author(s):  
Sriyani Rajapakse ◽  
Albert Abbott ◽  
John Kelly ◽  
Robert Ballard
Keyword(s):  
Escherichia Coli ◽  
Restriction Fragment Length Polymorphism ◽  
Dna Fingerprinting ◽  
Genomic Dna ◽  
Genetic Relatedness ◽  
Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
Rflp Markers ◽  
High Degree ◽  
Restriction Fragment Length

The feasibility of using RFLP to distinguish genetically related Hybrid Tea rose cultivars for DNA `fingerprinting' was examined with a group of cultivars related to `Peace'. The following cultivars used in this study, `Chicago Peace', `Flaming Peace', `Climbing Peace' and `Lucky Piece', were derived from bud mutations (sports) of `Peace'. We also investigated two additional cultivars, `Perfume Delight' and `Garden Party', in which one of the parents for each was `Peace'. Genomic rose DNA probes, cloned in pUC8 plasmid of Escherichia coli, were hybridized with genomic DNA of these cultivars digested with different restriction enzymes. Although polymorphisms were observed among these related cultivars, only a few probe/enzyme combinations screened produced RFLPs due to the high degree of genetic relatedness of these cultivars. We have identified probes that can distinguish all of these related rose cultivars. This study demonstrates that RFLP markers can be used effectively in DNA `fingerprinting' of genetically related rose cultivars, eventhough the level of detectable polymorphism is quite low.

scholarly journals Generation of a Highly Saturated Linkage Map in Peach [Prunus persica (L.) Batsch] Using AFLP and RFLP Markers

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 592b-592
Author(s):  
Bryon Sosinski ◽  
W.V. Baird ◽  
S. Rajapakse ◽  
R.E. Ballard ◽  
A.G. Abbott
Keyword(s):  
Linkage Map ◽  
Genetic Linkage Map ◽  
Prunus Persica ◽  
Quality Characteristics ◽  
Soluble Solids ◽  
Rflp Markers ◽  
Linkage Groups ◽  
Flesh Color ◽  
Peach Genome ◽  
The Cross

We have developed a highly saturated genetic linkage map in peach (diploid, 2n = 16) using two separate crosses. The first population consists of 48 randomly selected F2 individuals which were generated by selfing an F1 from the cross of `New Jersey Pillar' x KV 77119. This progeny set exhibits segregation for gross morphological traits including: canopy shape, fruit flesh color, and flower petal color, size, and number. The second population contains 48 F2 progeny derived from the cross of `Suncrest' x `Bailey'. These progeny segregate for quality traits such as fruit diameter, weight, flesh color, cling vs. free stone, soluble solids, pH of juice extract, and fruit developmental period. Nine linkage groups were identified in the first cross, which cover 590 cM of the genome. In the second cross, eight linkage groups were found that contain several significant chromosomal intervals contributing to fruit quality characteristics by QTL analysis. Anchor loci present in both maps were used to join the linkage groups to create a single combined map of the peach genome. Physical mapping is currently underway to assign the each linkage group to the appropriate chromosome.

Integrated maps of the chromosomes in Dictyostelium discoideum.

Genetics ◽  
1995 ◽  
Vol 141 (1) ◽  
pp. 147-157 ◽  
Author(s):  
W F Loomis ◽  
D Welker ◽  
J Hughes ◽  
D Maghakian ◽  
A Kuspa
Keyword(s):  
Molecular Weight ◽  
Dictyostelium Discoideum ◽  
Fragment Length Polymorphism ◽  
Restriction Enzymes ◽  
Restriction Map ◽  
Linkage Groups ◽  
High Molecular Weight Dna ◽  
Restriction Sites ◽  
Insertion Sites ◽  
Restriction Fragment Length

Abstract Detailed maps of the six chromosomes that carry the genes of Dictyostelium discoideum were constructed by correlating physically mapped regions with parasexually determined linkage groups. Chromosomally assigned regions were ordered and positioned by the pattern of altered fragment sizes seen in a set of restriction enzyme mediated integration-restriction fragment length polymorphism (REMI-RFLP) strains each harboring an inserted plasmid that carries sites recognized by NotI, SstI, SmaI, BglI and ApaI. These restriction enzymes were used to digest high molecular weight DNA prepared from more than 100 REMI-RFLP strains and the resulting fragments were separated and sized by pulsed-field gels. More than 150 gene probes were hybridized to blots of these gels and used to map the insertion sites relative to flanking restriction sites. In this way, we have been able to restriction map the 35 mb genome as well as determine the map position of more than 150 genes to with approximately 40 kb resolution. These maps provide a framework for subsequent refinement.

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