Detection of a variable number of ribosomal DNA loci by fluorescent in situ hybridization in Populus species

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 1020-1026 ◽  
Author(s):  
E. A. Prado ◽  
P. Faivre-Rampant ◽  
C. Schneider ◽  
M. A. Darmency
Keyword(s):  
Image Analysis ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Populus Deltoides ◽  
5S Rdna ◽  
Rflp Analysis ◽  
Variable Number ◽  
Populus Balsamifera ◽  
Populus Species

Fluorescent in situ hybridization (FISH) was applied to related Populus species (2n = 19) in order to detect rDNA loci. An interspecific variability in the number of hybridization sites was revealed using as probe an homologous 25S clone from Populus deltoides. The application of image analysis methods to measure fluorescence intensity of the hybridization signals has enabled us to characterize major and minor loci in the 18S–5.8S–25S rDNA. We identified one pair of such rDNA clusters in Populus alba; two pairs, one major and one minor, in both Populus nigra and P. deltoides; and three pairs in Populus balsamifera, (two major and one minor) and Populus euroamericana (one major and two minor). FISH results are in agreement with those based on RFLP analysis. The pBG13 probe containing 5S sequence from flax detected two separate clusters corresponding to the two size classes of units that coexist within 5S rDNA of most Populus species. Key words : Populus spp., fluorescent in situ hybridization, FISH, rDNA variability, image analysis.

scholarly journals Fluorescent in situ hybridization of 18S and 5S rDNA in papaya (Carica papaya l.) and wild relatives

Caryologia ◽  
2008 ◽  
Vol 61 (4) ◽  
pp. 411-416 ◽  
Author(s):  
Costa Fabiane R. ◽  
Telma N. S. Pereira ◽  
George L. Hodnett ◽  
Messias G. Pereira ◽  
David M. Stelly
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Carica Papaya ◽  
5S Rdna ◽  
Wild Relatives

Identification of alien chromosomes through GISH and RFLP analysis and the potential for establishing potato lines with monosomic additions of tomato chromosomes

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 666-673 ◽  
Author(s):  
F. Garriga-Calderé ◽  
D. J. Huigen ◽  
F. Filotico ◽  
E. Jacobsen ◽  
M. S. Ramanna
Keyword(s):  
In Situ Hybridization ◽  
Somatic Hybrids ◽  
Rflp Analysis ◽  
Chromosome Elimination ◽  
Variable Number ◽  
Substitution Lines ◽  
Chromosome Addition ◽  
Tomato Chromosome ◽  
Complete Series

To increase the potential for establishing a complete series of tomato chromosome addition–sbstitution lines in a potato background, six new BC1 progeny were produced. All of them originated from crosses between three different hexaploid potato (+) tomato fusion hybrids. Three different somatic hybrids, viz., C31-17-5, C31-17-24, and C31-17-51, were used as female parents, and four different tetraploids, viz., Katahdin, Frieslander, 6704-1, and AM66.42 were used as male parents. A characterisation of the genomes of the three fusion hybrids and the six BC1 progenies (6739, 2001, 2002, 2003, 2004, and 2005) through genomic in situ hybridization and restriction fragment length polymorphism (RFLP) analysis indicated that there was preferential tomato chromosome elimination in the fusion hybrids. Similar analyses of the six BC1 progeny indicated that a variable number of the alien tomato chromosomes (6–11) were present in individual plants. RFLP analysis using chromosome specific DNA probes indicated that BC1 progenies had retained all 12 tomato chromosomes, albeit in different individual plants. This means that the available BC1 progenies have the potential for establishing a complete series of tomato chromosome addition–substitution lines in a potato background.Key words: protoplast fusion hybrids, Solanum tuberosum, Lycopersicon esculentum, BC1 progeny, in situ hybridization, RFLP analysis.

Fluorescent in situ hybridization with rDNA probes on chromosomes of Acipenser ruthenus and Acipenser naccarii (Osteichthyes Acipenseriformes)

Genome ◽  
1999 ◽  
Vol 42 (5) ◽  
pp. 1008-1012 ◽  
Author(s):  
F Fontana ◽  
M Lanfredi ◽  
M Chicca ◽  
L Congiu ◽  
J Tagliavini ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
5S Rdna ◽  
28S Rdna ◽  
Detailed Characterization ◽  
Acipenser Ruthenus ◽  
Sturgeon Species ◽  
Sterlet Acipenser Ruthenus

The genes for 28S and 5S rDNA were physically mapped on the chromosomes of two sturgeon species, the sterlet (Acipenser ruthenus, 2n = 118 ± 4) and the Adriatic sturgeon (Acipenser naccarii, 2n = 248 ± 4) by fluorescent in situ hybridization. In the sterlet, the 28S rDNA was located on six chromosomes, four of which actively transcribed, while in the Adriatic sturgeon the 28S rDNA was located on a chromosome number ranging from 10 to 12, eight of which actively transcribed. The 5S rDNA was physically mapped on two chromosomes in the sterlet and on four in the Adriatic sturgeon. A more detailed characterization of the latter karyotype was obtained during this study. All these data are discussed in connection with the ploidy relationships among sturgeon species.Key words: karyotype, ploidy, FISH, 28S and 5S rDNA.

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