Recent advances in image analysis of chromosomes subjected to HG-banding and fluorescent in situ hybridization (FISH)

2002 ◽  
Author(s):  
A. Novak ◽  
A. Jovanovic
Keyword(s):  
Image Analysis ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Recent Advances

Detection of a variable number of ribosomal DNA loci by fluorescent in situ hybridization in Populus species

Genome ◽  
1996 ◽  
Vol 39 (5) ◽  
pp. 1020-1026 ◽  
Author(s):  
E. A. Prado ◽  
P. Faivre-Rampant ◽  
C. Schneider ◽  
M. A. Darmency
Keyword(s):  
Image Analysis ◽  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Populus Deltoides ◽  
5S Rdna ◽  
Rflp Analysis ◽  
Variable Number ◽  
Populus Balsamifera ◽  
Populus Species

Fluorescent in situ hybridization (FISH) was applied to related Populus species (2n = 19) in order to detect rDNA loci. An interspecific variability in the number of hybridization sites was revealed using as probe an homologous 25S clone from Populus deltoides. The application of image analysis methods to measure fluorescence intensity of the hybridization signals has enabled us to characterize major and minor loci in the 18S–5.8S–25S rDNA. We identified one pair of such rDNA clusters in Populus alba; two pairs, one major and one minor, in both Populus nigra and P. deltoides; and three pairs in Populus balsamifera, (two major and one minor) and Populus euroamericana (one major and two minor). FISH results are in agreement with those based on RFLP analysis. The pBG13 probe containing 5S sequence from flax detected two separate clusters corresponding to the two size classes of units that coexist within 5S rDNA of most Populus species. Key words : Populus spp., fluorescent in situ hybridization, FISH, rDNA variability, image analysis.

1796: Analysis of Chromosomal Aneuploidy in Patients with Oligoasthenoteratozoospermia(OAT) Using Fluorescent in-situ Hybridization

2007 ◽  
Vol 177 (4S) ◽  
pp. 596-597
Author(s):  
Joseph P. Alukal ◽  
Bobby B. Najari ◽  
Wilson Chuang ◽  
Lata Murthy ◽  
Monica Lopez-Perdomo ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Chromosomal Aneuploidy
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