Linkage relationships of allozyme-encoding loci in shiitake, Lentinula edodes

Genome ◽  
1991 ◽  
Vol 34 (4) ◽  
pp. 652-657 ◽  
Author(s):  
Christine G. Bowden ◽  
Daniel J. Royse ◽  
Bernie May
Keyword(s):  
Linkage Map ◽  
Lentinula Edodes ◽  
Recombination Frequency ◽  
Single Locus ◽  
Edible Fungi ◽  
Single Spore ◽  
Joint Segregation ◽  
The Individual ◽  
Hybrid Lines ◽  
Linkage Relationships

Single spore derived isolates from two parental and seven hybrid lines of Lentinula edodes were analyzed for single locus and joint segregation of 21 allozyme-encoding loci. The two alleles at the individual loci departed significantly from a 1:1 Mendelian ratio in 11% of the tests. With the exception of the highly significant χ2 value for Aat-1 in line WC131 (χ2 = 41.78), aberrant ratios were marginally significant and probably were due to chance alone. Eight linkages were identified: Ada ~ Gda, recombination frequency (r) = 0.15; Ada ~ Est, r = 0.12; Est ~ Gpi, r = 0.29; Ada ~ Gpi, r = 0.35; Gdh ~ Sod-1, r = 0.18; Mpi ~ PepG1-2, r = 0.13; Mpi ~ Sod-2, r = 0.04; and Sod-2 ~ PepG1-2, r = 0.14. Twelve loci were not shown to be linked to any other loci (Aat-1, Ak, Cat, Dia, Gk, β-Glu-1, β -Glu-2, Mdh, PepLgg-2, Pgd, Pgm, and Np). The first linkage map of 10 allozyme-encoding loci for the L. edodes genome is presented.Key words: shiitake, Lentinula edodes, allozymes, linkage map, edible fungi.

Linkage relationships of 19 allozyme encoding loci within the commercial mushroom genus Pleurotus

Genome ◽  
1988 ◽  
Vol 30 (6) ◽  
pp. 888-895 ◽  
Author(s):  
Bernie May ◽  
Kathrine J. Henley ◽  
Christine G. Fisher ◽  
Daniel J. Royse
Keyword(s):  
Linkage Map ◽  
Interspecific Hybrids ◽  
Single Locus ◽  
Linkage Groups ◽  
Edible Fungi ◽  
Single Spore ◽  
Joint Segregation ◽  
The Individual ◽  
Duplicate Loci ◽  
Linkage Relationships

Single spore derived Pleurotus spp. isolates from four commercial lines (two P. sapidus, one P. florida, and one P. ostreatus) and from two interspecific hybrids (P. sajor-caju × P. sapidus) were analyzed for single locus and joint segregation of 25 allozyme encoding loci. The two alleles at the individual loci departed significantly in their segregation from a 1:1 Mendelian ratio in 26% of the intraspecific and 29% of the interspecific tests. Six linkage groups were identified as follows: Dia-1 ~ Est-5; Tpi ~ Pgd-2 ~ Skdh; (Fum) ~ Pgm-2 ~ Pgd-1 ~ PepLgg-1 ~ Gr-2; Ndh ~ Gr-1; Np ~ PepGl-1 ~ Aat-2 ~ Pgk ~ Mup; and Gr-4 ~ Mdh-1. The duplicate loci coding for GR, PEP-LGG, PGM, and PGD were both not linked to each other and not part of duplicate linkage groups. Six loci were not shown to be linked to any other loci (Lap, Pgm-1, Ha, Gpi, PepPap, and PepLgg-2), although the latter two loci were only tested against four and five loci, respectively. The first linkage map of 19 allozyme encoding loci for the Pleurotus genome is presented.Key words: Pleurotus, allozymes, linkage map, inheritance, edible fungi.

scholarly journals NEW MUTATIONS AND A 7-CHROMOSOME LINKAGE MAP OF SCHIZOPHYLLUM COMMUNE

Genetics ◽  
1977 ◽  
Vol 85 (3) ◽  
pp. 417-425
Author(s):  
Carl Frankel ◽  
Albert H Ellingboe
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Group Analysis ◽  
Schizophyllum Commune ◽  
Somatic Recombination ◽  
Linkage Relationships ◽  
New Mutations

ABSTRACT Forty-eight useful new mutations of S. commune were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Their requirements and meiotic linkage relationships to each other and previously mapped areas were investigated. Several of these new mutations were incorporated into diploid strains so that the diploids contained at least one marker on every linkage group. Analysis of somatic recombination in these diploids indicated that each meiotic linkage group corresponded to an independent chromosome.

scholarly journals Construction of a Genetic Linkage Map in Tetraploid Species Using Molecular Markers

Genetics ◽  
2001 ◽  
Vol 157 (3) ◽  
pp. 1369-1385 ◽  
Author(s):  
Z W Luo ◽  
C A Hackett ◽  
J E Bradshaw ◽  
J W McNicol ◽  
D Milbourne
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Recombination Frequency ◽  
Simulated Data ◽  
Likelihood Estimation ◽  
Lod Score ◽  
Data Set ◽  
Independent Segregation ◽  
The Em Algorithm ◽  
Small Set

Abstract This article presents methodology for the construction of a linkage map in an autotetraploid species, using either codominant or dominant molecular markers scored on two parents and their full-sib progeny. The steps of the analysis are as follows: identification of parental genotypes from the parental and offspring phenotypes; testing for independent segregation of markers; partition of markers into linkage groups using cluster analysis; maximum-likelihood estimation of the phase, recombination frequency, and LOD score for all pairs of markers in the same linkage group using the EM algorithm; ordering the markers and estimating distances between them; and reconstructing their linkage phases. The information from different marker configurations about the recombination frequency is examined and found to vary considerably, depending on the number of different alleles, the number of alleles shared by the parents, and the phase of the markers. The methods are applied to a simulated data set and to a small set of SSR and AFLP markers scored in a full-sib population of tetraploid potato.

scholarly journals Inheritance and Linkage Relationships of Isozymes in Easter Cactus

1998 ◽  
Vol 123 (1) ◽  
pp. 98-103
Author(s):  
Maureen C. O'Leary ◽  
Thomas H. Boyle
Keyword(s):  
Interspecific Hybrids ◽  
Triosephosphate Isomerase ◽  
Recombination Frequency ◽  
Polyacrylamide Gel Electrophoresis ◽  
Interspecific Crosses ◽  
Germplasm Collections ◽  
Segregation Ratios ◽  
Isozyme Loci ◽  
Linkage Relationships ◽  
Aberrant Segregation

Polyacrylamide gel electrophoresis was used to study inheritance and linkage of isozymes in Easter cactus (Hatiora species and interspecific hybrids). Five isozyme systems were analyzed: aspartate aminotransferase (AAT), glucose-6-phosphate isomerase (GPI), malate dehydrogenase (MDH), phosphoglucomutase (PGM), and triosephosphate isomerase (TPI). F1, F2, BC1, and S1 progeny were used for inheritance studies. Six polymorphic loci (Aat-1, Gpi-1, Mdh-1, Pgm-1, Pgm-2, and Tpi-2) were identified. Aat-1 and Pgm-1 were linked (recombination frequency = 26% ± 7%), but the other isozyme loci assorted independently. Aberrant segregation ratios were observed in at least one segregating family for all six isozyme loci. We hypothesize that segregation distortion was due to linkage between isozyme loci and other genes subject to pre- or postzygotic selection. The existence of five additional isozyme loci (Aat-2, Gpi-2, Mdh-2, Mdh-3, and Tpi-1) was inferred from segregation patterns and by comparison of isozyme profiles from phylloclades and pollen. These isozyme loci may prove useful for confirming hybridity in intra- and interspecific crosses, determining parentage of cultivars, and assessing genetic diversity in germplasm collections.

scholarly journals Linkage relationships of markers on chromosome 17 of the house mouse

1975 ◽  
Vol 26 (2) ◽  
pp. 203-211 ◽  
Author(s):  
Graig Hammerberg ◽  
Jan Klein
Keyword(s):  
House Mouse ◽  
Chromosomal Region ◽  
Recombination Frequency ◽  
Genetic Distances ◽  
Chromosome 17 ◽  
Linkage Data ◽  
Crossing Over ◽  
The Right ◽  
Linkage Relationships ◽  
Important Marker

SUMMARYLinkage data for the following markers on chromosome 17 of the house mouse were obtained: centromere (marked by translocation R67), Brachyury (T), tufted (tf), H-2, and thin fur (thf). The markers were found to be arranged in that order in the genetic map and the combined genetic distances between individual markers were found to be as follows: Rb7…T, 4·5 cM; T…tf, 5·8 cM; tf…H-2, 5·0 cM; H-2…thf, 15·1 cM. The localization of the thf locus on the non-centromeric side of the H-2 complex provides an important marker for this arm of chromosome 17. The map distances in the centromeric portion of chromosome 17 changed drastically in the presence of various t factors. These factors strongly reduce the recombination frequency in the T…tf and tf…H-2 intervals and this crossing-over suppression is most likely responsible for the linkage disequilibrium between t and H-2 reported earlier. Recombinants involving a t chromosome but occurring to the right of the H-2 complex do not change the properties of t factors suggesting that all determinants responsible for the t phenotype are located in the chromosomal region between T and tf (H-2).

Variation in size and shape of physogastry of Dolichocybe perniciosa (Acari: Dolichocybidae)

2019 ◽  
Vol 24 (8) ◽  
pp. 1526-1532
Author(s):  
Qingxiu Lan ◽  
Bingrong Ke ◽  
Jianhua Liao ◽  
Zhenghui Lu ◽  
Qing-Hai Fan
Keyword(s):  
Agaricus Bisporus ◽  
Lentinula Edodes ◽  
Pleurotus Eryngii ◽  
Width Ratio ◽  
Strong Variation ◽  
Edible Fungi ◽  
Auricularia Auricula ◽  
Suitable Temperature ◽  
Length Width ◽  
Shape And Size

The formation of physogastry of the mushroom mite Dolichocybe perniciosa (Acari: Prostigmata: Dolichocybidae) has not been well understood. The shape and size of this mite vary dramatically. To evaluate the effects of environmental factors on the formation of physogastry we tested eight species of edible fungi hyphae, five temperatures and five humidity levels on the shape and size of physogastry. Dolichocybe perniciosa only fed on six species of edible fungi, Agaricus bisporus, Auricularia auricula-judae, Auricularia polytricha, Flammulina velutipes, Ganoderma lucidum and Lentinula edodes but failed to develop on the hyphae of Pleurotus geesteranus and Pleurotus eryngii. However, the six species of edible fungi had different effects on the formation and development of physogastry in D. perniciosa. The suitable temperature for the physogastric formation was from 18°C to 28°C at 75±5% RH, and the suitable humidity was from 22% to 92% at 25°C. The width of physogastries was less than 0.85 mm at five temperatures. The majority of physogastries were oblong or cylindrical under ideal temperature (25°C), but globular at a low temperature (13°C). The largest length and the highest length/width ratio were recorded at 25°C. The length and width of the physogastry were less than 4.0 mm and 0.8 mm, respectively, under tested humidity levels. The highest length/width ratio was 8.19 at 65% RH.

Construction of a Genetic Linkage Map Using RAPD, ISSR, SRAP and SSR Markers for Lentinula Edodes

2013 ◽  
Vol 1 (2) ◽  
pp. 142-150
Author(s):  
Shuiming Cheng ◽  
Fanxue Lin ◽  
Junren Zhao ◽  
Hanbing Han
Keyword(s):  
Linkage Map ◽  
Ssr Markers ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Lentinula Edodes

scholarly journals Chromosome-Wide Characterization of Intragenic Crossover in Shiitake Mushroom, Lentinula edodes

2021 ◽  
Vol 7 (12) ◽  
pp. 1076
Author(s):  
Wenbing Gong ◽  
Nan Shen ◽  
Lin Zhang ◽  
Yinbing Bian ◽  
Yang Xiao
Keyword(s):  
Lentinula Edodes ◽  
Critical Role ◽  
Central Component ◽  
Nucleotide Polymorphisms ◽  
Single Spore ◽  
Single Nucleotide ◽  
Genome Wide ◽  
Cis And Trans ◽  
Qtls Mapping

Meiotic crossover plays a critical role in generating genetic variations and is a central component of breeding. However, our understanding of crossover in mushroom-forming fungi is limited. Here, in Lentinula edodes, we characterized the chromosome-wide intragenic crossovers, by utilizing the single-nucleotide polymorphisms (SNPs) datasets of an F1 haploid progeny. A total of 884 intragenic crossovers were identified in 110 single-spore isolates, the majority of which were closer to transcript start sites. About 71.5% of the intragenic crossovers were clustered into 65 crossover hotspots. A 10 bp motif (GCTCTCGAAA) was significantly enriched in the hotspot regions. Crossover frequencies around mating-type A (MAT-A) loci were enhanced and formed a hotspot in L. edodes. Genome-wide quantitative trait loci (QTLs) mapping identified sixteen crossover-QTLs, contributing 8.5–29.1% of variations. Most of the detected crossover-QTLs were co-located with crossover hotspots. Both cis- and trans-QTLs contributed to the nonuniformity of crossover along chromosomes. On chr2, we identified a QTL hotspot that regulated local, global crossover variation and crossover hotspot in L. edodes. These findings and observations provide a comprehensive view of the crossover landscape in L. edodes, and advance our understandings of conservation and diversity of meiotic recombination in mushroom-forming fungi.

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