In situ localization of a mammalian protamine gene: parameters affecting specificity of hybridization

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 459-463 ◽  
Author(s):  
Stephen A. Krawetz ◽  
Manfred H. Herfort ◽  
Gordon H. Dixon
Keyword(s):  
Genomic Dna ◽  
Southern Hybridization ◽  
Chromosomal Localization ◽  
Chromosomal Location ◽  
Cdna Probe ◽  
Chromosome 1 ◽  
Telomeric Region ◽  
Southern Hybridization Analysis ◽  
Indian Muntjac

Southern hybridization analysis of Indian muntjac genomic DNA with the Eco-Taq bovine genomic protamine probe revealed a simple banding pattern. The pattern of hybridization was identical with that previously observed in the genus Bos. This suggested that the bovine probe specifically hybridized to the Indian muntjac protamine gene. The opportunity was thus provided to assign the chromosomal location of the protamine gene in a comparatively simple system. Accordingly, this probe and the corresponding cDNA probe were used for in situ chromosome hybridization and localization. Various parameters affecting specificity and the resolution of hybridization were examined. Subsequent to optimization, the Indian muntjac gene was shown to be autosomal and distally located in the telomeric region of the p arm of chromosome 1.Key words: chromosomal localization, muntjac, protamine, specificity, hybridization.

scholarly journals Interindividual size heteromorphism of NOR and chromosomal location of 5S rRNA genes in Iheringichthys labrosus

2007 ◽  
Vol 50 (1) ◽  
pp. 141-146 ◽  
Author(s):  
Rafael Augusto de Carvalho ◽  
Ana Lúcia Dias
Keyword(s):  
Chromosomal Location ◽  
5S Rdna ◽  
Rrna Genes ◽  
Telomeric Region ◽  
Fluorescent Staining ◽  
Homologous Chromosomes ◽  
Rdna Probe ◽  
5S Rrna Genes ◽  
Iheringichthys Labrosus

Twenty-five specimens of Iheringichthys labrosus from the Capivara Reservoir were analysed cytogenetically. AgNORs were detected in a pair of ST chromosomes, in the telomeric region of the long arm. Some individuals showed size heteromorphism of this region between homologous chromosomes. Treatment with CMA3 displayed GC-rich regions corresponding to the AgNOR pair, plus other fluorescent staining. In situ hybridization by fluorescence (FISH) with the 18S rDNA probe showed only one pair of stained chromosomes, confirming the heteromorphism observed with AgNO3 and CMA3 in some individuals. The 5S rDNA probe revealed telomeric staining on the long arm of a pair of chromosomes of the ST-A group, probably different from the NOR pair.

scholarly journals Assignment of the mouse desmin gene to chromosome 1 band C3

1990 ◽  
Vol 55 (2) ◽  
pp. 101-105 ◽  
Author(s):  
Zhenlin Li ◽  
Marie-Geneviève Mattei ◽  
Jean-François Mattei ◽  
Denise Paulin
Keyword(s):  
Human Chromosome ◽  
Gene Cluster ◽  
Intermediate Filament ◽  
Chromosomal Localization ◽  
Chromosome 1 ◽  
Chromosome 2 ◽  
Gene Coding ◽  
Partial Homology ◽  
Conserved Gene

SummaryThe chromosomal localization of the mouse gene coding for desmin, one of the muscle-specific intermediate filament subunits, was determined by in situ hybridization using a specific 3H-labelled DNA probe. There is only one copy of the desmin gene and it is located on chromosome 1 in the band C3. This result adds an eleventh locus to a conserved gene cluster and confirms the partial homology that exists between the long arm of human chromosome 2 and chromosome 1 of the mouse.

scholarly journals Recognition of adenovirus types in faecal samples by Southern hybridization in South Australia

1994 ◽  
Vol 112 (3) ◽  
pp. 603-613 ◽  
Author(s):  
L. D. Mickan ◽  
T.-W. Kok
Keyword(s):  
Enzyme Immunoassay ◽  
Genomic Dna ◽  
Southern Hybridization ◽  
South Australia ◽  
Late Winter ◽  
Viral Gastroenteritis ◽  
Southern Hybridization Analysis ◽  
Restriction Patterns ◽  
Faecal Samples ◽  
Enteric Adenovirus

SUMMARYThe distribution of adenovirus types in faecal samples of patients with suspected viral gastroenteritis from South Australia was determined during the 12-month period, July 1991–June 1992. There were 3299 samples tested and 226 (6·9%) were positive for adenovirus by enzyme immunoassay. Of these 226 samples. 154 (68%) were typed directly using virus DNA extracted from the faecal samples according to theSmaI,HindIII andBstEII restriction patterns and Southern hybridization analysis with pooled viral genomic DNA probes. In this group, 86% of the samples were from patients who were < 3 years of age. Enteric adenovirus types 40 and 41 accounted for 20 and 40% respectively, of these samples, and types 1, 2, 3, 5, 6, 7 and 31 comprised the remainder. Type 40 was detected mainly in the winter and spring periods, and type 41 predominated in the autumn period. The majority of the non-enteric types were found during the late winter and spring periods.

Cloning, characterization, and chromosomal localization of human liver form cytochrome c oxidase subunit VIa related genes

Genome ◽  
1997 ◽  
Vol 40 (3) ◽  
pp. 325-331 ◽  
Author(s):  
Frank Merante ◽  
Mingfu Ling ◽  
Catherine Duff ◽  
Brian H. Robinson ◽  
Alessandra M. V. Duncan
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Human Liver ◽  
Somatic Cell Hybrid ◽  
Chromosomal Localization ◽  
Chromosomal Location ◽  
Chromosome 6 ◽  
Human Cytochrome ◽  
Human Cytochrome C

The chromosomal location of human cytochrome c oxidase (COX) subunit VIa Liver (VIa-L) isoform related sequences has been determined by a combination of in situ hybridization and analysis of human–hamster somatic cell hybrid panels. COX VIa-L related sequences were present on chromosomes 6 and 12. It has been verified that at least two COX VIa-L genes are on chromosome 6, one of which is a pseudogene. In total, four COX VIa-L related sequences have been cloned and their nucleotide sequences analyzed. At least three of these sequences represent pseudogenes; their relatedness to the COX VIa-L cDNA is discussed.Key words: human, cytochrome c oxidase, chromosomal localization, COX VIa, cloning.

scholarly journals Chromosomal localization of the mouse gene coding for vimentin

1989 ◽  
Vol 53 (3) ◽  
pp. 183-185 ◽  
Author(s):  
Marie-Genevieve Mattei ◽  
Alain Lilienbaum ◽  
Li Zhen Lin ◽  
Jean-François Mattei ◽  
Denise Paulin
Keyword(s):  
Intermediate Filament ◽  
Chromosomal Localization ◽  
Chromosomal Location ◽  
Dna Probes ◽  
Mouse Gene ◽  
Chromosome 2 ◽  
Gene Coding ◽  
Vimentin Gene

SummaryThe chromosomal location of the mouse gene coding for vimentin, one of the intermediate filament subunits, was determined by in situ hybridization using specific H3-labelled DNA probes. There is only one copy of the vimentin gene and it is located on chromosome 2 region A2.

scholarly journals Molecular cloning of cDNAs for the human granulocyte colony-stimulating factor receptor from HL-60 and mapping of the gene to chromosome region 1p32-34

Blood ◽  
1992 ◽  
Vol 79 (5) ◽  
pp. 1148-1154 ◽  
Author(s):  
DJ Tweardy ◽  
K Anderson ◽  
LA Cannizzaro ◽  
RA Steinman ◽  
CM Croce ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Cdna Library ◽  
Cell Lines ◽  
Cdna Probe ◽  
Chromosome 1 ◽  
Human Leukemia ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Stimulating Factor

Abstract Early studies examining the effects of purified or recombinant granulocyte colony-stimulating factor (G-CSF) on human leukemia cell lines demonstrated that some cell lines, such as HL-60, could be induced to differentiate in response to G-CSF. In two recent studies reporting the cloning of the human G-CSF receptor (hGCSFR), four classes of receptor cDNA were identified and, surprisingly, the message for this receptor was reportedly expressed by HL-60 at either very low levels or not at all. Using a mouse G-CSF receptor probe, we cloned and sequenced a cDNA for hGCSFR from an HL-60 cDNA library in plasmid and used it to identify 31 additional clones from an HL-60 cDNA library in phage. Polymerase chain reaction analysis of the 31 phage clones established that 29 were derived from class I hGCSFR mRNA, one was derived from class III mRNA, and one was derived from class IV mRNA. In addition, the hGCSFR gene was chromosomally localized by Southern blot analysis of its segregation pattern in a panel of rodent-human hybrid DNAs using the radiolabeled cDNA probe. The hGCSFR locus was present in hybrids retaining the distal short arm of human chromosome 1 and absent in hybrids that did not retain this region. Chromosomal in situ hybridization refined the localization of the hGCSFR gene to region 1p32-p34. The combination of hybrid DNA analysis and in situ hybridization places the hGCSFR gene telomeric to the CSF1, JUN, and TCL-5 loci.

Isolation, characterization, and analysis of Leymus-specific DNA sequences

Genome ◽  
2003 ◽  
Vol 46 (4) ◽  
pp. 673-682 ◽  
Author(s):  
Sigridur Klara Bödvarsdóttir ◽  
Kesara Anamthawat-Jónsson
Keyword(s):  
Dna Sequences ◽  
Genomic Dna ◽  
Southern Hybridization ◽  
Genetic Relatedness ◽  
Repetitive Sequences ◽  
Nucleolar Organiser Regions ◽  
Basic Genome ◽  
Specific Sequences ◽  
Leymus Mollis

Genomic Southern hybridization using labeled total genomic DNA of Leymus mollis as probe showed intense hybridization signals on all restriction enzyme digested DNA from five species of Leymus Hochst., and four species of Psathyrostachys Nevski. Experiments using the same L. mollis probe, but with unlabeled blocking DNA from Psathyrostachys, showed no hybridization at all. These two genera evidently had the same genomic content. Southern hybridization without blocking allowed identification of DNA fragments abundant in Leymus and Psathyrostachys. Fragments potentially specific to Leymus were cloned. Five repetitive DNA clones from L. mollis and L. arenarius were characterized: pLmIs1, pLmIs44, pLmIs51, pLmIs53, and pLaIs56. These clones hybridized to both Leymus and Psathyrostachys on Southern blots — no clone hybridized to only one of these genera. Both Southern blot and fluorescence in situ hybridization (FISH) experiments showed that all the clones contained dispersed repetitive sequences. They painted all and whole chromosomes uniformly except at centromeres, telomeres, and nucleolar organiser regions. Three of these clones, i.e., pLmIs1, pLmIs44, and pLmIs53, were essentially specific to Leymus and Psathyrostachys — little or no hybridization was detected in other genera such as Triticum, Hordeum, Thinopyrum, or Elymus. Sequence analysis further revealed that the clones were part of retroelements. In particular, the clone pLmIs44 produced hybridization profiles suitable for analysis of genetic relatedness among species. The present study shows that Leymus and Psathyrostachys share the same basic genome, Ns, and therefore provides strong evidence for combining these two genera.Key words: Triticeae, Leymus, Psathyrostachys, genome-specific sequences, retrotransposons.

scholarly journals Molecular cloning of cDNAs for the human granulocyte colony-stimulating factor receptor from HL-60 and mapping of the gene to chromosome region 1p32-34

Blood ◽  
1992 ◽  
Vol 79 (5) ◽  
pp. 1148-1154
Author(s):  
DJ Tweardy ◽  
K Anderson ◽  
LA Cannizzaro ◽  
RA Steinman ◽  
CM Croce ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Cdna Library ◽  
Cell Lines ◽  
Cdna Probe ◽  
Chromosome 1 ◽  
Human Leukemia ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Stimulating Factor

Early studies examining the effects of purified or recombinant granulocyte colony-stimulating factor (G-CSF) on human leukemia cell lines demonstrated that some cell lines, such as HL-60, could be induced to differentiate in response to G-CSF. In two recent studies reporting the cloning of the human G-CSF receptor (hGCSFR), four classes of receptor cDNA were identified and, surprisingly, the message for this receptor was reportedly expressed by HL-60 at either very low levels or not at all. Using a mouse G-CSF receptor probe, we cloned and sequenced a cDNA for hGCSFR from an HL-60 cDNA library in plasmid and used it to identify 31 additional clones from an HL-60 cDNA library in phage. Polymerase chain reaction analysis of the 31 phage clones established that 29 were derived from class I hGCSFR mRNA, one was derived from class III mRNA, and one was derived from class IV mRNA. In addition, the hGCSFR gene was chromosomally localized by Southern blot analysis of its segregation pattern in a panel of rodent-human hybrid DNAs using the radiolabeled cDNA probe. The hGCSFR locus was present in hybrids retaining the distal short arm of human chromosome 1 and absent in hybrids that did not retain this region. Chromosomal in situ hybridization refined the localization of the hGCSFR gene to region 1p32-p34. The combination of hybrid DNA analysis and in situ hybridization places the hGCSFR gene telomeric to the CSF1, JUN, and TCL-5 loci.

scholarly journals Associations between Amplification (1q) and Prior Cancer in a Real-World De Novo Myeloma Cohort

2021 ◽  
Author(s):  
Elizabeth B Lamont ◽  
Andrew J Yee ◽  
Stuart L Goldberg ◽  
David S Siegel ◽  
Andrew D Norden
Keyword(s):  
Real World ◽  
Fluorescent In Situ Hybridization ◽  
De Novo ◽  
Chromosome 1 ◽  
Second Malignancies ◽  
Cancer History ◽  
Screening Strategies ◽  
Cytogenetic Testing

Abstract Genomic biomarkers inform treatment in multiple myeloma (MM) making patient clinical data a potential window into MM biology. We evaluated de novo MM patients for associations between specific MM cytogenetic patterns and prior cancer history. Analyzing a MM real-world dataset (RWD), we identified a cohort of 1,769 patients with fluorescent in-situ hybridization (FISH) cytogenetic testing at diagnosis. Fully 241 patients (0.14) had histories of prior cancer(s). Amplification of the long arm of chromosome 1 [amp(1q)] varied by prior cancer history (0.31 with prior cancer vs 0.24 without; p = .02). No other MM translocations, amplifications, or deletions were associated with prior cancers. Amp(1q) and cancer history remained strongly associated in a logistic regression adjusting for patient demographic and disease attributes. The results merit follow-up regarding carcinogenic treatment effects and screening strategies for second malignancies. Broadly the findings suggest analyses of patient-level phenotypic-genomic RWD may accelerate cancer research through hypothesis generating studies.

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