Localization of the 68 000-Da human neurofilament gene (NF68) using a murine cDNA probe

Genome ◽  
1988 ◽  
Vol 30 (4) ◽  
pp. 499-500 ◽  
Author(s):  
Martin J. Somerville ◽  
Donald R. McLachlan ◽  
Maire E. Percy
Keyword(s):  
Centromeric Region ◽  
Human Gene ◽  
Cdna Probe ◽  
Chromosome 8 ◽  
Chromosome 7 ◽  
Gene Localization ◽  
Chromosome 2 ◽  
Genomic Probe ◽  
Human Genomic

A recent investigation, using a human genomic probe, has indicated that the 68 000 dalton neurofilament gene (NF68) is on the short arm of chromosome 8. We have used a murine cDNA probe on 65 metaphase spreads in situ to localize the human NF68 gene to 8p21 (20/370 grains; p < 0.0001). In addition, we have found secondary hybridization sites at the centromeric region of chromosome 2 and the long arm of chromosome 7, which are putative loci for other intermediate filaments.Key words: neurofilament, human, gene localization, murine cDNA.

scholarly journals The human interleukin-1 alpha gene is located on the long arm of chromosome 2 at band q13

Blood ◽  
1989 ◽  
Vol 73 (1) ◽  
pp. 104-107 ◽  
Author(s):  
M Lafage ◽  
N Maroc ◽  
P Dubreuil ◽  
R de Waal Malefijt ◽  
MJ Pebusque ◽  
...  
Keyword(s):  
Interleukin 1 ◽  
Cdna Probe ◽  
Proximal Part ◽  
Chromosome 2 ◽  
Interleukin 1 Beta ◽  
Hematopoietic Growth Factors ◽  
Alpha Gene ◽  
Immunologic Reactions ◽  
Beta Gene

Abstract Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which was described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. In this report we discuss our use of in situ hybridization on human prometaphase cells with a human IL-1 alpha cDNA probe to localize the human IL-1 alpha gene on the proximal part of the long arm of chromosome 2 at band q13, in the same chromosomal region as the IL-1 beta gene.

scholarly journals Single and joint effect of the basal region of chromosome 2 and centromeric region of chromosome 8 on morphological and fruit quality traits in tomato

Euphytica ◽  
2016 ◽  
Vol 210 (3) ◽  
pp. 327-339 ◽  
Author(s):  
Gisela Yael Green ◽  
Javier Hernán Pereira da Costa ◽  
Vladimir Cambiaso ◽  
Guillermo Raúl Pratta ◽  
Roxana Zorzoli ◽  
...  
Keyword(s):  
Fruit Quality ◽  
Centromeric Region ◽  
Joint Effect ◽  
Chromosome 8 ◽  
Basal Region ◽  
Quality Traits ◽  
Chromosome 2 ◽  
Fruit Quality Traits

scholarly journals Gene amplification accompanies low level increases in the activity of dihydrofolate reductase in antifolate-resistant Chinese hamster lung cells containing abnormally banding chromosomes.

1982 ◽  
Vol 94 (2) ◽  
pp. 418-424 ◽  
Author(s):  
J A Lewis ◽  
J L Biedler ◽  
P W Melera
Keyword(s):  
Dihydrofolate Reductase ◽  
Cdna Probe ◽  
Chinese Hamster ◽  
Hybridization Technique ◽  
Chromosome 2 ◽  
Dhfr Gene ◽  
Low Level ◽  
Amplification Process ◽  
Staining Techniques

Three independently-derived, antifolate-resistant Chinese hamster lung cell lines that exhibit low level increases in dihydrofolate reductase (DHFR) activity, i.e., three- to fivefold vs. controls, have been compared with drug-sensitive cells to determine relative DHFR gene content. With a solution hybridization technique that makes use of genomic DNA and a cloned double-stranded Chinese hamster DHFR cDNA probe, it has been found that the enzyme activity increases are associated with an approximately proportionate amplification of DHFR genes. Trypsin-Giemsa staining techniques and hybridizations in situ further show that the amplified DHFR genes are located within abnormally banding regions along chromosome 2q and also suggest that, in each subline, only one chromosome 2 homolog is initially involved in the amplification process.

Cytological evidence of terminal deficiencies produced by the r-X1 deletion in maize

Genome ◽  
1987 ◽  
Vol 29 (5) ◽  
pp. 718-721 ◽  
Author(s):  
Bor-yaw Lin
Keyword(s):  
Key Words ◽  
Centromeric Region ◽  
Chromosome 8 ◽  
Chromosome 2 ◽  
Cytological Evidence ◽  
Chromosome Breaks ◽  
Key Words Maize ◽  
The Cross

In addition to monosomes and trisomes, the r-X1 deletion generates chromosome deficiencies of parts of an arm; these deficiencies now have been identified cytologically. Of eight japonica plants produced from the cross of r-X1/R-r as female with the Mangelsdorf's tester, five were complete and three were partial monosomes. The isolated partial monosomes had chromosome breaks in the region between the centromere and the japonica locus. Their breakpoints were at the 0.33, 0.30, and 0.25 positions, respectively, on the long arm of chromosome 8. Additionally, a liguleless plant produced from the same cross carried a partial monosome with a breakpoint at the 0.09 position on the short arm of chromosome 2. Although partial monosomes associated with other chromosome arms have not been investigated, it is evident that the action of the r-X1 deletion is not restricted to the centromeric region of maize chromosomes. Key words: maize, r-X1 deletion, terminal deficiencies, monosomes.

scholarly journals The human interleukin-1 alpha gene is located on the long arm of chromosome 2 at band q13

Blood ◽  
1989 ◽  
Vol 73 (1) ◽  
pp. 104-107
Author(s):  
M Lafage ◽  
N Maroc ◽  
P Dubreuil ◽  
R de Waal Malefijt ◽  
MJ Pebusque ◽  
...  
Keyword(s):  
Interleukin 1 ◽  
Cdna Probe ◽  
Proximal Part ◽  
Chromosome 2 ◽  
Interleukin 1 Beta ◽  
Hematopoietic Growth Factors ◽  
Alpha Gene ◽  
Immunologic Reactions ◽  
Beta Gene

Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which was described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. In this report we discuss our use of in situ hybridization on human prometaphase cells with a human IL-1 alpha cDNA probe to localize the human IL-1 alpha gene on the proximal part of the long arm of chromosome 2 at band q13, in the same chromosomal region as the IL-1 beta gene.

scholarly journals Interphase cytogenetic analysis of in vivo differentiation in the myelodysplasia of Down syndrome

Blood ◽  
1994 ◽  
Vol 84 (7) ◽  
pp. 2278-2282 ◽  
Author(s):  
A Zipursky ◽  
H Wang ◽  
EJ Brown ◽  
J Squire
Keyword(s):  
Down Syndrome ◽  
Chromosomal Abnormalities ◽  
Chromosome 8 ◽  
Chromosome 7 ◽  
Leukemic Cells ◽  
Trisomy 8 ◽  
Monosomy 7 ◽  
Megakaryoblastic Leukemia

Abstract In Down syndrome, acute megakaryoblastic leukemia (AMKL) occurs frequently during the first 4 years of life and is usually preceded by a period of myelodysplasia (MDS), often associated with chromosomal abnormalities. Archival peripheral blood and/or bone marrow films of six patients with Down syndrome and MDS whose leukemic cells contained monosomy 7 or trisomy 8 were studied to determine whether the abnormal precursors produce mature cells in vivo. Using fluorescence in situ hybridization (FISH) of interphase nuclei with chromosome-specific centromere probes for either chromosome 7 or 8, we were able to determine which cells had one, two, or three signals indicative of one, two, or three no. 7 or 8 chromosomes. In five patients with trisomy 8, 80% to 100% (94.5% +/- 6.2%) of the megakaryoblasts had three signals using a chromosome 8 probe; in one patient with monosomy 7, 96.5% of the megakaryoblasts had one signal using a chromosome 7 probe. In all six patients, the myeloid and lymphoid series did not have evidence of the chromosomal abnormality present in the blasts. In three of five patients with trisomy 8, three signals were observed in 27%, 33%, and 41% of normoblasts, respectively. These data are evidence that the abnormal cell in MDS is a progenitor cell with the potential of forming cells of megakaryocyte and erythroid lineages.

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